• Title/Summary/Keyword: DsbA

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Conserved Virulence Factors of Pseudomonas aeruginosa are Required for Killing Bacillus subtilis

  • Park Shin-Young;Heo Yun-Jeong;Choi Young-Seok;Deziel Eric;Cho You-Hee
    • Journal of Microbiology
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    • v.43 no.5
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    • pp.443-450
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    • 2005
  • The multi-host pathogen, Pseudomonas aeruginosa, possesses an extraordinary versatility which makes it capable of surviving the adverse conditions provided by environmental, host, and, presumably, competing microbial factors in its natural habitats. Here, we investigated the P. aeruginosa-Bacillus subtilis interaction in laboratory conditions and found that some P. aeruginosa strains can outcompete B. subtilis in mixed planktonic cultures. This is accompanied by the loss of B. subtilis viability. The bactericidal activity of P. aeruginosa is measured on B. subtilis plate cultures. The bactericidal activity is attenuated in pqsA, mvfR, lasR, pilB, gacA, dsbA, rpoS, and phnAB mutants. These results suggest that P. aeruginosa utilizes a subset of conserved virulence pathways in order to survive the conditions provided by its bacterial neighbors.

Study on the WTO Disputes over the Korean Shipbuilding Industry in Relation to Export Credit (수출신용과 관련하여 우리나라 조선산업에 대한 WTO 무역분쟁 연구)

  • Lee, Koung-Rae
    • Korea Trade Review
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    • v.44 no.1
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    • pp.129-142
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    • 2019
  • This paper draws implications on the ship finance of the Korean ECAs for shipbuilding industry from the perspective of WTO ASCM through studying the trade disputes on export credits. In consideration of the underwriting practice on the case-by-case basis, the ECAs' law regimes and their ship finance programs as such would be judged not conferring a benefit. The ship finance of international commercial banks could be treated as a market benchmark for the purpose of determining the existence of benefit in the ECA ship finance. The ECAs share securities with international commercial banks for the same exposure to the risks in a syndicate. Therefore, WTO DSB would rule that the ECA ship finance confers no benefit for individual transactions. The items (j) and (k-1) of ASCM Annex I are not allowed to interpret a contrario.

The design of 85GHz-115Ghz band SIS mixer for the observing cosmic radio waves (85GHz-115Ghz 대 우주전파 관측용 초전도체 믹서 설계)

  • 한석태;김효령;이창훈;박종애;정현수;김광동;김태성;박동철
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.6
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    • pp.90-98
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    • 1996
  • We have evaluated the theoretical conversion loss and noise temperature of mixer using the quantum mixer theory and the method to determine the embedding impedance of waveguide-type mixer mount. At fixed backshort position of the mixer, the calculated SSB mixer conversion loss and mixer noise temperature are 5 dB and 10K within frequency range form 85 GHz to 115 GHz, respectively. The SIS mixer has been developed by using through on the calculated rsutls to observe cosmic radio waves. SIS junction of mixer is Nb/Al-AlOx/Nb and it consists of four series array. Area of each of junction is about 2.5${\mu}m^{2}$. The average receiver noise temperature of manufactured receiver with this mixer is about 30 K(DSB). The receiver noise temperature is much lower than that of receiver with a mixer using mechanical tuning backshort.

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A Correlation between Emile Sound and Other Waves (에밀레의 맥놀이와 다른 파동과의 상관관계)

  • 안정근;진용옥
    • The Journal of the Acoustical Society of Korea
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    • v.20 no.1
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    • pp.30-35
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    • 2001
  • The most important characteristic of Emile Bell's sound is a beating. It is modulation phenomenon which appears as a result of interference multiplication in time domain. This modulation phenomenon can be modeled as DSB-SC which suppress carrier and signals distributed both sides. The beatiog wave is observed in Laman distribution signal for polyvinyl speech signal, water vein wave, tide wave. The beating wave is caused by asymmetry Property of the bell.

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Status of ASTE Focal Plane Array Development

  • Lee, Jung-Won;Je, Do-Heung;Lee, Bangwon;Kang, Hyunwoo;Wagner, Jan;Kim, Jongsoo;Han, Seog-Tae;Asayama, Shin'ichiro;Kojima, Takafumi;Gonzalez, Alvaro;Kroug, Matthias;Shan, Wenrei;Iguchi, Satoru;Iono, Daisuke
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.59.2-59.2
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    • 2016
  • As an enhancement to increase mapping speed of the current ALMA TP array, development of a focal plane array system working at ultra wide frequency range of 275-500 GHz with GPU-based software spectrometers has been carried out since 2015. Major progresses on such component development as wideband DSB mixers, a profiled corrugated horn, receiver optics, LO system and GPU-based spectrometer are reviewed with brief introduction to implication of ALMA 2030 for technical implementation.

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Dielectric properties of ZnO-based varistor(2) (ZnO 바리스터의 유전특성(2))

  • Kang, Dae-Ha;Rho, Il-Soo;Park, Sang-Ho;Hur, Jin-Seuk;Choi, Pil-Soo
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.1267-1268
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    • 2007
  • In this study a low-signal equivalent circuit based on DSB model is proposed for ZnO-based varistor. As a equivalent circuit simulation result dielectric loss factor $tan{\delta}$ shows 2 peaks in low frequency and high frequency regions. The low-frequency peak means the relaxation by deep defects and the high-frequency peak means the relaxation by shallow defects.

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Study on Characteristics of the Forward Link Signal for the UHF RFID Reader (UHF 대역 RFID 리더의 순방향 링크 신호 특성에 관한 연구)

  • Kim, Do-Yun;Jang, Byung-Jun;Yoon, Hyun-Goo;Park, Jun-Seok;Yook, Jong-Gwan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.18 no.6 s.121
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    • pp.602-611
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    • 2007
  • In this paper, the forward link of UHF RFID system is modeled in accordance with the EPCglobal class 1 generation 2(EPCglobal C1G2) UHF Radio-Frequency Identity protocol specification at $860{\sim}960MHz$. Based on the constructed model, characteristics on the forward link signal for the EPCglobal C1G2 RFID reader are simulated with the help of a MATLAB softwarein order to extract the design parameters of a transmit digital filter which meets the Korean RFID regulations. Herein, the forward link model is consisted of PIE source coding, transmit digital filter, modulation, local oscillator, and antenna. From the simulation results, the ranges of three design parameters(roll-off factor, cutoff frequency, the number of tabs) for transmit digital filter are obtained with different modulation techniques and the Tari(type a reference interval) values. Finally, DSB/SSB-ASK modulation technique can not satisfy the EPCglobal C1G2 specification when Tari equals to $6.25{\mu}sec$ in a multiple-reader environment. Consequently this paper can provide a guideline for design parameters of a RFID reader as well as the basic scheme of analyzing frequency interference problems in RFID environments, including multiple-reader and dense-reader environments.

Text Mining Analysis Technique on ECDIS Accident Report (텍스트 마이닝 기법을 활용한 ECDIS 사고보고서 분석)

  • Lee, Jeong-Seok;Lee, Bo-Kyeong;Cho, Ik-Soon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.4
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    • pp.405-412
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    • 2019
  • SOLAS requires that ECDIS be installed on ships of more than 500 gross tonnage engaged in international navigation until the first inspection arriving after July 1, 2018. Several accidents related to the use of ECDIS have occurred with its installation as a new major navigation instrument. The 12 incident reports issued by MAIB, BSU, BEAmer, DMAIB, and DSB were analyzed, and the cause of accident was determined to be related to the operation of the navigator and the ECDIS system. The text was analyzed using the R-program to quantitatively analyze words related to the cause of the accident. We used text mining techniques such as Wordcloud, Wordnetwork and Wordweight to represent the importance of words according to their frequency of derivation. Wordcloud uses the N-gram model as a way of expressing the frequency of used words in cloud form. As a result of the uni-gram analysis of the N-gram model, ECDIS words were obtained the most, and the bi-gram analysis results showed that the word "Safety Contour" was used most frequently. Based on the bi-gram analysis, the causative words are classified into the officer and the ECDIS system, and the related words are represented by Wordnetwork. Finally, the related words with the of icer and the ECDIS system were composed of word corpus, and Wordweight was applied to analyze the change in corpus frequency by year. As a result of analyzing the tendency of corpus variation with the trend line graph, more recently, the corpus of the officer has decreased, and conversely, the corpus of the ECDIS system is gradually increasing.

Shear Bond Strength of a 3-in-1 Flowable Composite Resin to Primary Teeth (유치에 대한 3-in-1 유동성 복합레진의 전단결합강도)

  • Lee, Hyeongjik;Shin, Jonghyun;Kim, Jiyeon;Jeong, Taesung;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.45 no.4
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    • pp.436-444
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    • 2018
  • The purpose of this study was to compare the shear bond strengths of 3-in-1 flowable composite to the enamel and dentin of primary teeth to previous adhesive systems. 110 primary incisors were prepared and divided into two groups: Dentin group included 5 groups: 1) Scotch bond Multi-purpose plus(DSM), 2) Single-bond 2(DSB), 3) Clearfil SE bond(DSE), 4) All bond universal(DAB), 5) Constic(DC), and Enamel group included 6 groups: 1) Scotch bond Multi-purpose plus(ESM), 2) Single bond 2(ESB), 3) Clearfil SE bond(ESE), 4) All bond universal(EAB), 5) Constic(EC), 6) Constic with additional etching(ECE). A cylinder of composite was bonded to the prepared surface, and the shear bond strength was measured. In the dentin groups, group DC had significantly lower shear bond strength than group DSE. No significant difference was found between group DC, group DSM, group DSB and group DAB. In the enamel groups, there was no significant difference between group EC, group ESE, and group EAB. This material showed lowest shear bond strength among all tested materials in both enamel and dentin groups, showing insignificant difference with some adhesive systems. Therefore, 3-in-1 flowable composite can be used for primary teeth restoration but further studies are needed.

Involvement of Brca1 in DNA Interstrand Cross-link Repair Through Homologous Recombination-independent Process (재조합 비의존적 경로를 통한 DNA 사슬간 교차결합 복구에의 Brca1단백질의 기능)

  • Yun, Jean-Ho
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.542-547
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    • 2005
  • Hypersensitivity of cells lacking Brcal to DNA interstrand .ross-link (ICL) agents such as cisplatin and mitomycin C(MMC) implicates the important role of Brcal in cellular response following ICL treatment. Brca1 plays an essential role in DNA double-strand break (DSB) repair through homologous recombination (HR)-dependent and -independent process. Recently, our group has been reported that Brca1 involves in cellular ICL response through HR-dependent repair process (Yun J. et at., Oncogene 2005). In this report, the involvement of Brca1 protein in HR-independent repair process is examined using isogenic $p53^{-/-}\;and\;p53^{-/-}\;Brcal^{-/-}$ mouse embryonic fibroblast (MEF) and psoralen cross-linked reporter reactivation assay. Brcal-deficient MEFs showed significantly low HR-independent repair activity compare to Brca1-proficient MEFs. Hypersensitivity to MMC and ICL reporter repair activity were restored by the reconstitution of Brca1 expression. Interestingly, MEFs expressing exon 11-deleted isoform of Brca1 $(Brca1^{\Delta11/\Delta11})$ showed high resistance to MMC and ICL reporter repair activity comparable to Brca1-reconstituted MEFs. Taken together, these results suggest that Brca1 involves in ICL repair through not only HR-dependent process but also HR-independent process using N-terminal RINC finger domain or C-terminal BRCT domain rather than exon 11 region which mediate interaction with Rad50.