• Asian-Australasian Journal of Animal Sciences
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• v.30 no.12
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• pp.1702-1710
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• 2017

Objective: The study examined the effect of condensed tannins (CT) containing Ficus infectoria and Psidium guajava leaf meal mixture (LMM) supplementation on nutrient metabolism, methane emission and performance of lambs. Methods: Twenty four lambs of ~6 months age (average body weight $10.1{\pm}0.60kg$) were randomly divided into 4 dietary treatments (CT-0, CT-1, CT-1.5, and CT-2 containing 0, 1.0, 1.5, and 2.0 percent CT through LMM, respectively) consisting of 6 lambs each in a completely randomized design. All the lambs were offered a basal diet of wheat straw ad libitum, oat hay (100 g/d) along with required amount of concentrate mixture to meet their nutrient requirements for a period of 6 months. After 3 months of experimental feeding, a metabolism trial of 6 days duration was conducted on all 24 lambs to determine nutrient digestibility and nitrogen balance. Urinary excretion of purine derivatives and microbial protein synthesis were determined using high performance liquid chromatography. Respiration chamber study was started at the mid of 5th month of experimental feeding trial. Whole energy balance trials were conducted on individual lamb one after the other, in an open circuit respiration calorimeter. Results: Intake of dry matter and organic matter (g/d) was significantly (p<0.05) higher in CT-1.5 than control. Digestibility of various nutrients did not differ irrespective of treatments. Nitrogen retention and microbial nitrogen synthesis (g/d) was significantly (p<0.01) higher in CT-1.5 and CT-2 groups relative to CT-0.Total body weight gain (kg) and average daily gain (g) were significantly (linear, p<0.01) higher in CT-1.5 followed by CT-1 and CT-0, respectively. Feed conversion ratio (FCR) by lambs was significantly (linear, p<0.01) better in CT-1.5 followed by CT-2 and CT-0, respectively. Total wool yield (g; g/d) was linearly (p<0.05) higher for CT-1.5 than CT-0. Methane emission was linearly decreased (p<0.05) in CT groups and reduction was highest (p<0.01) in CT-2 followed by CT-1.5 and CT-1. Methane energy (kcal/d) was linearly decreased (p<0.05) in CT groups. Conclusion: The CT supplementation at 1% to 2% of the diet through Ficus infectoria and Psidium guajava LMM significantly improved nitrogen metabolism, growth performance, wool yield, FCR and reduced methane emission by lambs.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.100-110
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• 2020

Objective: The objective of this study was to isolate proteolytic microorganisms and evaluate their effects on proteolysis in total mixed ration (TMR) silages of soybean curd residue. Methods: TMRs were formulated with soybean curd residue, alfalfa or Leymus chinensis hay, corn meal, soybean meal, a vitamin-mineral supplement, and salt in a ratio of 25.0: 40.0:30.0:4.0:0.5:0.5, respectively, on a basis of dry matter. The microbial proteinases during ensiling were characterized, the dominate strains associated with proteolysis were identified, and their enzymatic characterization were evaluated in alfalfa (A-TMR) and Leymus chinensis (L-TMR) TMR silages containing soybean curd residue. Results: Both A-TMR and L-TMR silages were well preserved, with low pH and high lactic acid concentrations. The aerobic bacteria and yeast counts in both TMR silages decreased to about 10⁵ cfu/g fresh matter (FM) and below the detection limit, respectively. The lactic acid bacteria count increased to 10⁹ cfu/g FM. The total microbial proteinases activities reached their maximums during the early ensiling stage and then reduced in both TMR silages with fermentation prolonged. Metalloproteinase was the main proteinase when the total proteinases activities reached their maximums, and when ensiling terminated, metallo and serine proteinases played equally important parts in proteolysis in both TMR silages. Strains in the genera Curtobacterium and Paenibacillus were identified as the most dominant proteolytic bacteria in A-TMR and L-TMR, respectively, and both their proteinases were mainly with metalloproteinase characteristics. In the latter ensiling phase, Enterococcus faecium strains became the major sources of proteolytic enzymes in both TMR silages. Their proteinases were mainly of metallo and serine proteinases classes in this experiment. Conclusion: Proteolytic aerobic bacteria were substituted by proteolytic lactic acid bacteria during ensiling, and the microbial serine and metallo proteinases in these strains played leading roles in proteolysis in TMR silages.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.2
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• pp.5-14
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• 2017

Humic acid was used soil amendment or functional fertilizer in Korean agriculture, and its cation exchangeable capacity was high enough to increase soil buffering from plant toxicant. This study was conducted to evaluate effects of humic acid powder (HA) on composting of livestock manure (LM) and of its application on growth of lettuce. Treatments were designed as follows; livestock manure compost (LM+sawdust+bark+castor meal; Control), control+0.1% HA (0.1% HA), control+0.5% HA (0.5% HA), control+1.0% HA (1.0% HA), control+3.0% HA (3.0% HA), and control+5.0% HA (5.0% HA). The changes of temperature, water content, organic matter content, total nitrogen and ratio of organic matter and nitrogen in HA treatments were similar to those of control. Although pH of 3.0% HA and 5.0% HA blending with HA and LM was lower than those of others, it unaffected by HA blending during composting. Humic acid content of HA treatments was increased by 1.7~4.4 folds than that of control. As compared with odor index, 3.0% HA and 5% HA were decreased than control for composting time. Application of 3.0% HA increased the dry weight of lettuce by about 7% than that of control.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.533-540
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• 2019

Objective: This study evaluated the growth, digestibility and rumen fermentation between goats and sheep fed a fattening diet fortified with linseed oil. Methods: Twelve 3 to 4 months old male goats and sheep were randomly allocated into two dietary treatment groups in a $2(species){\times}2$ (oil levels) factorial experiment. The treatments were: i) goats fed basal diet, ii) goats fed oil-supplemented diet, iii) sheep fed basal diet, and iv) sheep fed oil-supplemented diet. Each treatment group consisted of six animals. Animals in the basal diet group were fed with 30% alfalfa hay and 70% concentrates at a rate equivalent to 4% of their body weight. For the oil treatment group, linseed oil was added at 4% level (w:w) to the concentrate portion of the basal diet. Growth performance of the animals was determined fortnightly. Digestibility study was conducted during the final week of the feeding trial before the animals were slaughtered to obtain rumen fluid for rumen fermentation characteristics study. Results: Sheep had higher (p<0.01) average daily weight gain (ADG) and better feed conversion ratio (FCR) than goats. Oil supplementation did not affect rumen fermentation in both species and improved ADG by about 29% and FCR by about 18% in both goats and sheep. The above enhancement is consistent with the higher dry matter and energy digestibility (p<0.05), as well as organic matter and neutral detergent fiber digestibility (p<0.01) in animals fed oil- supplemented diet. Sheep had higher total volatile fatty acid production and acetic acid proportion compared to goat. Conclusion: The findings of this study suggested that sheep performed better than goats when fed a fattening diet and oil supplementation at the inclusion rate of 4% provides a viable option to significantly enhance growth performance and FCR in fattening sheep and goats.

• Animal Bioscience
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• v.35 no.2
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• pp.217-223
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• 2022

Objective: The aim of this study was to determine the effects of feeding starch sugar byproducts (SSBs) on in situ disappearance rate, performance, and carcass characteristics of Hanwoo steers in the late finishing stage. Methods: To determine the in situ disappearance rate, nylon bags filled with 5 g of SSB were inserted into the ventral sac of two cannulated Holsteins cows and incubated for 0, 2, 4, 8, 16, 24, and 48 h. A total of 30 Hanwoo steers were fed the experimental diets, which were basal diet (control) and 7% SSB on an as-fed basis (4.35% dry matter [DM]), formulated according to requirements of the Korean Feeding Standard for Hanwoo. The experiment was conducted over 80 days using a completely randomized block design. Results: Soluble fraction a of DM and organic matter (OM) was 44.20% and 64.60% DM, fraction b was 23.00% and 19.40% DM, and c values (the rate of degradation of fraction b) were 0.04 and 0.04/h, respectively. The effective degradability of DM at rumen solid outflow rates of 0.02, 0.05, and 0.08/h was 59.83, 54.75, and 52.16, respectively, and for OM was 77.78, 73.52, and 71.34, respectively. Initial and final body weight, average daily gain, DM intake, and gain:feed did not differ significantly between control and SSB groups during the entire experimental period. Carcass traits of Hanwoo steers with SSB supplementation were not significantly different between treatments except for dressing percentage, which was greater with SSB treatment. The content of saturated fatty acid (SFA) was greater and that of unsaturated fatty acids (UFA) was lower in the SSB group than in the control group. The ratio of UFA to SFA was significantly lower in the SSB group than in the control group. Conclusion: A total mixed ration containing less than 4.0% DM of SSBs can be used in Hanwoo steers without a decrease in productivity and carcass traits.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.114-124
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• 2022

The Saemangeum has a dry surface characteristic with a low moisture content ratio due to the saline and silt soil, so the vegetation cover is low compared to other areas. In areas with low vegetation cover, wind erosion has a high probability of scattering dust. If the vegetation cover is increased by cultivating crops that can withstand the Saemangeum reclaimed environment, scattering dust can be reduced by reducing the flow rate at the bottom. Thus, the purpose of this study is to analyze the effect of suppressing the generation of fine dust and scattering dust by cultivating winter forage crops on the Saemangeum reclaimed land. While growing 0.5 ha of barley and 0.5 ha of triticale in Saemangeum reclaimed land, the concentration of fine dust was monitored according to agricultural work and growth stage. Changes in the concentrations of PM-10, PM-2.5, and PM-1.0 were monitored on the leeward, the windward and centering on the crop field. As a result of monitoring, PM-1.0 had little effect on crop cultivation. the concentration of PM-10 and PM-2.5 increased according to tillage and harvesting, and tillage had a higher increasing the concentration of PM-10 and PM-2.5 than that of harvesting. According to the growth stage of crops, the effect of suppressing scattering dust was shown, and the effect of suppressing scattering dust was higher in the heading stage than in the seedling stage. So, it was found that there was an effect of suppressing scattering dust other than the effect of land covering. Through this study, it was possible to know about the generation and suppression effect of scattering dust according to crop cultivation.

• Animal Bioscience
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• v.35 no.6
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• pp.838-846
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• 2022

Objective: Native plants can be used as additives to replace antibiotics to improve ruminant feed utilization and animal health. An experiment was conducted to evaluate the effects of Gentiana straminea (GS) on nutrient digestibility, methane emissions, and energy metabolism of Simmental calves. Methods: Thirty-two (5-week-old) male Simmental clves, with initial body weight (BW) of 155±12 kg were fed the same basal diet of concentrates (26%), alfalfa hay (37%), and oat hay (37%) and were randomly separated into four treatment groups according to the amount of GS that was added to their basal diet. The four different groups received different amounts of GS as a supplement to their basal diet during whole experiment: (0 GS) 0 mg/kg BW, the control; (100 GS) 100 mg/kg BW; (200 GS) 200 mg/kg BW; and (300 GS) 300 mg/kg BW. Results: For calves in the 200 GS and 300 GS treatment groups, there was a significant increase in dry matter (DM) intake (p<0.01), average daily gain (ADG) (p<0.05), organic matter intake (p<0.05), DM digestibility (p<0.05), neutral detergent fibre (NDF) digestibility (p<0.05), and acid detergent fibre (ADF) digestibility (p<0.05). Dietary GS supplementation result in quadratic increases of DM intake (p<0.01), ADG (p<0.05), NDF intake (p<0.05), and ADF intake (p<0.05). Supplementing the basal diet with GS significantly increased nitrogen (N) retention (p<0.001) and the ratio of retention N to N intake (p<0.001). Supplementing the basal diet with GS significantly decreased methane (CH₄) emissions (p<0.01), CH₄/BW^0.75 (p<0.05) and CH₄ energy (CH₄-E) (p<0.05). Dietary GS supplementation result in quadratic increases of CH₄ (p<0.01) and CH₄/DM intake (p<0.01). Compared with 0 GS, GS-supplemented diets significantly improved their gross energy intake (p<0.05). The metabolizable energy and digestive energy intake were significantly greater for calves in the 100 GS and 200 GS calves than for 0 GS calves (p<0.05). Conclusion: From this study, we conclude that supplementing calf diets with GS could improve utilization of feed, energy, and N, and may reduce CH₄ emissions without having any negative effects on animal health.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.2
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• pp.117-124
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• 1994

A study was conducted to dectermine the days with optimum soil moisture after drainage or rain for machine workability and seeding establishment on dry seeding in paddy soils(Jeon buk Series, Haplaquepts). The results are summarized as follows. 1. The possible days of tractor-rotary plough were 5days or more for non-mole hole plot(control). The possible days were 2~4days and the optimum days were 5~6days for mole hale plat. 2. The ratio of soil clod(<1cm) was 1 % and 15% for control and mole hole plats, respectively. The optimum soil moisture range for seeding establishment was 31~32% in this soils. 3. The possible days of combine harvesting work were 9days and 5days after drainage for control and mole hole plots, respectively. 4. Soil physical properties were improved but soil organic matter calcium and magnesium in the soil were decreased in the mole hole plats. 5. Yield was 415kg/10a at seeding after 6day drainage in control and 440kg/10a at seeding after 4day drainage in the mole plots.

• Journal of Life Science
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• v.14 no.3
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• pp.396-399
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• 2004

This study was investigated the changes of root length, tissue structure of root tip, and dry matter production of a Dongjinbyeo (DJ) cultivar subjected to 0.4 % agar medium with various concentration of NaCl (salt stress)- and polyethylene glycol 6000 (PEG, drought stress). Root length and dry weight of DJ plant were declined along the high concentration of PEG and NaCl in rice plants. To elucidate the changes of tissue structure in root tip to PEG- and NaCl-treatments, we examined the microscopic observation of root tip in NaCl- and PEG-treated rice plants using Toluidine blue O. By Toluidine blue O staining, methyl-lignin accumulation was found in the epidermis and outer cortex of the elongation zone at an early stage of PEG treatment, whereas was found only the outer cortex of the elongation zone of NaCl-treated root tip. The epidermis of NaCl-treated root tip became soften instead of methyl-lignin accumulation. TR ratio was increased along the high concentration in PEG- and NaCl-treated rice plant as a result of inhibited root elongation under PEG- and NaCl-treatment. From these morphological changes in root stimulated by drought and salt stress, we suggest that agar medium is useful to identify tolerant variety in germination stage under stressful environments.

• Korean Journal of Medicinal Crop Science
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• v.11 no.2
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• pp.83-88
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• 2003

This experiment was carried out to investigate the effect of the mulching of hairy vetch and pig manure on the growth, yield and crude protein contents of the mulching of hairy vetch and application of animal slurry were treated and investigated in $2000{\sim}2002$. At the first year of cultivation, there were no differences among the treatments in plant height, but second year the growth of plants increased and continued over long times as the hairy vetch mulched and fertilized. The length and diameter of plant root were increased in the plot of hairy vetch mulching and the application of animal slurry. At first year of cultivation, no clear difference was found for among the treatments on dry weight and dry matter ratio in roots. At second year of cultivation, they were increased at in the plot of mulching and fertilizer treatment. The appearances of weeds in the field on Astragalus membranaces were much lower on the plots of live-mulching of hairy vetch than those on the control. The yield of root was 255kg highest in the plot of mulching and animal slurry application of 2-year-old roots. The root yield of Astragalus membranaces was increased about $8{\sim}10%$ compared to that with control, due to supply of nutrients. During the cultivation times, organic matters and N contents in soil increased by the mulching of hairy vetch and the application of animal slurry. The concentration of K, Ca concentration in soil showed a tendency to increase.