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http://dx.doi.org/10.5713/ajas.18.0933

Characteristics of proteolytic microorganisms and their effects on proteolysis in total mixed ration silages of soybean curd residue  

Hao, Wei (Department of Agricultural Engineering, College of Engineering, China Agricultural University)
Tian, Pengjiao (Department of Agricultural Engineering, College of Engineering, China Agricultural University)
Zheng, Mingli (Department of Agricultural Engineering, College of Engineering, China Agricultural University)
Wang, Huili (Department of Agricultural Engineering, College of Engineering, China Agricultural University)
Xu, Chuncheng (Department of Agricultural Engineering, College of Engineering, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.33, no.1, 2020 , pp. 100-110 More about this Journal
Abstract
Objective: The objective of this study was to isolate proteolytic microorganisms and evaluate their effects on proteolysis in total mixed ration (TMR) silages of soybean curd residue. Methods: TMRs were formulated with soybean curd residue, alfalfa or Leymus chinensis hay, corn meal, soybean meal, a vitamin-mineral supplement, and salt in a ratio of 25.0: 40.0:30.0:4.0:0.5:0.5, respectively, on a basis of dry matter. The microbial proteinases during ensiling were characterized, the dominate strains associated with proteolysis were identified, and their enzymatic characterization were evaluated in alfalfa (A-TMR) and Leymus chinensis (L-TMR) TMR silages containing soybean curd residue. Results: Both A-TMR and L-TMR silages were well preserved, with low pH and high lactic acid concentrations. The aerobic bacteria and yeast counts in both TMR silages decreased to about 105 cfu/g fresh matter (FM) and below the detection limit, respectively. The lactic acid bacteria count increased to 109 cfu/g FM. The total microbial proteinases activities reached their maximums during the early ensiling stage and then reduced in both TMR silages with fermentation prolonged. Metalloproteinase was the main proteinase when the total proteinases activities reached their maximums, and when ensiling terminated, metallo and serine proteinases played equally important parts in proteolysis in both TMR silages. Strains in the genera Curtobacterium and Paenibacillus were identified as the most dominant proteolytic bacteria in A-TMR and L-TMR, respectively, and both their proteinases were mainly with metalloproteinase characteristics. In the latter ensiling phase, Enterococcus faecium strains became the major sources of proteolytic enzymes in both TMR silages. Their proteinases were mainly of metallo and serine proteinases classes in this experiment. Conclusion: Proteolytic aerobic bacteria were substituted by proteolytic lactic acid bacteria during ensiling, and the microbial serine and metallo proteinases in these strains played leading roles in proteolysis in TMR silages.
Keywords
Aerobic Bacteria; Lactic Acid Bacteria; Proteolysis; Proteinase; Total Mixed Ration Silage;
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1 Tao L, Zhou H, Guo XS, Long RJ, Zhu Y, Cheng W. Contribution of exopeptidases to formation of nonprotein nitrogen during ensiling of alfalfa. J Dairy Sci 2011;94:3928-35. https://doi.org/10.3168/jds.2010-3752   DOI
2 Mckersie BD, Buchanan-Smith J. Changes in the levels of proteolytic enzymes in ensiled alfalfa forage. Can J Plant Sci 1982;62:111-6. https://doi.org/10.4141/cjps82-017   DOI
3 Winters AL, Cockburn JE, Dhanoa MS, Merry RJ. Effects of lactic acid bacteria in inoculants on changes in amino acid composition during ensilage of sterile and non-sterile ryegrass. J Appl Microbiol 2000;89:442-52. https://doi.org/10.1046/j.1365-2672.2000.01133.x   DOI
4 Heron SJE, Edwards RA, Mcdonald P. Changes in the nitrogenous components of gamma-irradiated and inoculated ensiled ryegrass. J Sci Food Agric 1986;37:979-85. https://doi.org/10.1002/jsfa.2740371005   DOI
5 Tao L, Li M, Guo X, Yang F, Zhou, H. Effect of epiphytic microorganisms and exogenous lactic acid bacteria on the formation of non-protein nitrogen during the ensiling of alfalfa. J Anim Vet Adv 2012;11;2181-6. https://doi.org/10.3923/javaa.2012.2181.2186   DOI
6 AOAC International. Official methods of analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemists; 1990.
7 Givens DI, Rulquin H. Utilisation by ruminants of nitrogen compounds in silage-based diets. Anim Feed Sci Technol 2004;114:1-18. https://doi.org/10.1016/j.anifeedsci.2003.09.005   DOI
8 Mcdonald P, Henderson AR. Determination of water-soluble carbohydrates in grass. J Sci Food Agric 1964;15:395-8. https:// doi.org/10.1002/jsfa.2740150609   DOI
9 Licitra G, Hernandez TM, Van Soest PJ. Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Technol 1996;57:347-58. https://doi.org/10.1016/0377-8401(95)00837-3   DOI
10 Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 1980;63:64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8   DOI
11 Fischer A, Brouquisse R, Raymond P. Influence of senescence and of carbohydrate levels on the pattern of leaf proteases in purple nutsedge (Cyperus rotundus). Physiol Plant 1998;102:385-95. https://doi.org/10.1034/j.1399-3054.1998.1020307.x   DOI
12 NC-IUBMB (Nomenclature Committee of the International Union of Biochemistry and Molecular Biology). Enzyme nomenclature. Orlando, FL, USA: Academic Press; 1992.
13 Sevinc N, Demirkan E. Production of protease by Bacillus sp. N-40 isolated from soil and its enzymatic properties. J Biol Environ Sci 2011;5:95-103.
14 Cavallarin L, Antoniazzi S, Tabacco E, Borreani G. Effect of the stage of growth, wilting and inoculation in field pea (Pisum sativum L.) silages. II. Nitrogen fractions and amino acid compositions of herbage and silage. J Sci Food Agric 2006;86:1383-90. https://doi.org/10.1002/jsfa.2526   DOI
15 Guo XS, Ding WR, Han JG, Zhou H. Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Anim Feed Sci Technol 2008;142:89-98. https://doi.org/10.1016/j.anifeedsci.2007.07.005   DOI
16 Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2   DOI
17 Alvarez VN, von der Weid I, Seldin L, Santos AL. Influence of growth conditions on the production of extracellular proteolytic enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2. Lett Appl Microbiol 2006;43:625-30. https://doi.org/10.1111/j.1472-765X.2006.02015.x   DOI
18 Mckersie BD, Buchanan-Smith J. Changes in the levels of proteolytic enzymes in ensiled alfalfa forage. Can J Plant Sci 1982;62:111-6. https://doi.org/10.4141/cjps82-017   DOI
19 Wallace RJ, Brammall ML, Wallace RJ, Brammall ML. The role of different species of bacteria in the hydrolysis of protein in the rumen. J Gen Microbiol 1985;131:821-32. https://doi.org/10.1099/00221287-131-4-821
20 Hang F, Liu P, Wang Q, et al. High milk-clotting activity expressed by the newly isolated Paenibacillus spp. strain BD3526. Molecules 2016;21:73. https://doi.org/10.3390/molecules21010073   DOI
21 Sai-Ut S, Benjakul S, Sumpavapol P, Kishimura H. Purification and Characterization of Extracellular Gelatinolytic Protease from Bacillus Amyloliquefaciens H11. J Food Biochem 2015;39:119-28. https://doi.org/10.1111/jfbc.12114   DOI
22 Law J, Haandrikman A. Proteolytic enzymes of lactic acid bacteria. Int Dairy J 1997;7:1-11. https://doi.org/10.1016/0958-6946(95)00073-9   DOI
23 Sasaki M, Boukje WB, Tan PST. Comparison of proteolytic activities in various lactobacilli. J Dairy Res 1995;62:601-10.   DOI
24 Liu S, Pritchard GG, Hardman MJ, Pilone GJ. Occurrence of arginine deiminase pathway enzymes in arginine catabolism by wine lactic acid bacteria. Appl Environ Microbiol 1995;61:310-6.   DOI
25 Papadopoulos YA, Mckersie BD. A comparison of protein degradation during wilting and ensiling of six forage species. Can J Plant Sci 1983;63:903-12. https://doi.org/10.4141/cjps83-114   DOI
26 Xu CC, Cai YM, Moriya N, Ogawa M. Nutritive value for ruminants of green tea grounds as a replacement of brewers' grains in totally mixed ration silage. Anim Feed Sci Technol 2007;138:228-38. https://doi.org/10.1016/j.anifeedsci.2006.11.014   DOI
27 Khalid NM, Marth EH. Proteolytic activity by strains of Lactobacillus plantarum and Lactobacillus casei. J Dairy Sci 1990;73:3068-76. https://doi.org/10.3168/jds.S0022-0302(90)78994-1   DOI
28 Hegazi FZ, Abo-Elnaga IG. Factors affecting the caseinolytic activity of Lactobacillus casei and Lactobacillus plantarum. Mol Nutr Food Res 1987;31:199-206. https://doi.org/10.1002/food.19870310303
29 Oke MA, Onilude AA. Partial purification and characterization of extracellular protease from Pedicoccus acidilactici. Nig J Basic Appl Sci 2014;22:19-25. http://dx.doi.org/10.4314/njbas.v22i1.4   DOI
30 El-Ghaish S, Dalgalarrondo M, Choiset Y, et al. Screening of strains of Lactococci isolated from Egyptian dairy products for their proteolytic activity. Food Chem 2010;120:758-64. https://doi.org/10.1016/j.foodchem.2009.11.007   DOI
31 Cao Y, Takahashi T, Horiguchi K. Effects of addition of food by-products on the fermentation quality of a total mixed ration with whole crop rice and its digestibility, preference, and rumen fermentation in sheep. Anim Feed Sci Technol 2009;151:1-11. https://doi.org/10.1016/j.anifeedsci.2008.10.010   DOI
32 McDonald P, Henderson AR, Heron SJE. The biochemistry of silage. 2nd ed. Welton, Lincoln, UK: Chalcombe Publications; 1991.
33 Hu XD, Hao W, Wang HL, Ning TT, Zheng ML, Xu CC. Fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace. Asian-Australas J Anim Sci 2015;28:502-510. https://doi.org/10.5713/ajas.14.0508   DOI
34 Hao W, Wang HL, Ning TT, Yang FY, Xu CC. Aerobic stability and effects of yeasts during deterioration of non-fermented and fermented total mixed ration with different moisture levels. Asian-Australas J Anim Sci 2015;28:816-26. https://doi.org/10.5713/ajas.14.0837   DOI
35 Weinberg ZG, Chen Y, Miron D, et al. Preservation of total mixed rations for dairy cows in bales wrapped with polyethylene stretch film - A commercial scale experiment. Anim Feed Sci Technol 2011;164:125-9. https://doi.org/10.1016/j.anifeedsci.2010.11.016   DOI
36 Ohshima M, Mcdonald P. A review of the changes in nitrogenous compounds of herbage during ensilage. J Sci Food Agric 1978;29:497-505. https://doi.org/10.1002/jsfa.2740290602   DOI
37 Guo XS, Cheng W, Zhang Y, Yang FY, Zhou H. Contribution of endopeptidases to the formation of nonprotein nitrogen during ensiling of alfalfa. Anim Feed Sci Technol 2011;168:42-50. https://doi.org/10.1016/j.anifeedsci.2011.03.018   DOI
38 Mckersie BD. Proteinases and peptidases of alfalfa herbage. Can J Plant Sci 1981;61:53-9. https://doi.org/10.4141/cjps81-008   DOI