• Parasites, Hosts and Diseases
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• v.47 no.4
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• pp.369-375
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• 2009

Diphyllobothrium nihonkaiense was first described by Yamane in 1986 but the taxonomical features have been obscure due to lack of critical morphologic criteria in its larval and adult stages. In Korea, this tapeworm had long been known as Diphyllobothrium latum. In this study, we observed 62 specimens collected from Korean residents and analyzed them by morphological features and nucleotide sequences of mitochondrial cox1 gene as well as the ITS1 region. Adult tapeworms were examined after carmine or trichrome stain. Longitudinal sections of the gravid proglottids showed an obtuse angle of about 150 degree between the cirrus sac and seminal vesicle. This angle is known as a major differential point compared with that of D. latum. Nucleotide sequence differences between D. latum and the specimens from Koreans represented 17.3% in mitochondrial DNA cox1 gene. Sequence divergence of ITS1 among 4 Korean isolates was 0.3% and similarity was 99.7% with D. nihonkaiense and D. klebanovskii. All of the Korean specimens analyzed in this study were identified as being D. nihonkaiense (n = 62). We propose its Korean name as "Dong-hae-gin-chon-chung" which means 'long tapeworm of the East Sea' for this newly analyzed diphyllobothriid tapeworm in Korea.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.399-405
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• 2001

Urushiol, a natural pro-electrophilic quinone compound, has potential structural characteristics as antitumor chemotherapeutic agents. However, urushiol's use as an antitumor drug has some problems, because it is hardly miscible with an aqueous solution. Purified urushiol is highly viscous and soluble only in strong solvents. for this study, we prepared an urushiol-ethanol micro-emulsion with a unimodal size distribution by high-speed homogenization. This generated effective delivery of urushiol to its action wites, so that we could investigate its cytotoxic activity against cancer cells. Using a colony-forming assay, we were able to show that urushiol selectively inhibited the growth of the ovarian cancer cells PA-1 and 2774 at a concentration of $10^{-6}$, whereas it had only a negligible effect on normal CHO cells at the same concentration. The data suggest that urushiol may have potential as an effective antitumor agent in the treatment of ovarian cancer. In addition, we addressed the question of whether the specific cytotoxic effect of urushiol is linked to apoptosis, by DNA fragmentation and DAPI staining assays. The inhibitory effects of urushiol on the growth of ovarian cancer cells was found to be associated with DNA fragmentation and the fragmented nuclei formation, both of which represent markers for the induction of apoptosis. Therefore, the results suggested that urushiol affected its profound cytotoxicity by triggering apoptosis in ovarian cancer cells.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.6
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• pp.379-384
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• 2005

A high-performance liquid chromatography assay method for oxolinic acid in fish products was developed, evaluated and validated through the monitoring of oxolinic acid based on farming and distribution. The recovery rate of the developed method was $102.3-106.7\%$ as compared to conventional methods. The stock solution was stable for 3 weeks under refrigerated condition at $4^{\circ}C$ The performance limit was evaluated as 0.01ppm of oxolinic acid in fish muscle. 478 fish samples such as olive flounder, genuine porgy, common sea bass and black rock fish collected from fish farms in the coastal area from September 2001 to October 2004 were analyzed to evaluate overall efficiency of the modified method and to monitor the actual condition of oxolinic acid usage in fish farm. According to the monitoring results, the modified method was suitable for analysis of oxolinic acid in fish muscle and oxolinic acid might be used in a small portion of fish farms. The suggested analysis method of oxolinic acid was registered in the Korean Official Methods of Food Analysis and is being utilized for fishery products by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.

• Korean Journal of Plant Resources
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• v.4 no.2
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• pp.67-74
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• 1991

Many people have a growing interest on the health result from economic improvement, various environimental pollution and stress, and unrest on the adult diseases etc.In these result, demand for the herb medicine continues to expand. Farmers of theour country have a hardship in the farm management owing to of opening and liberaliza-tion, and make strenuous efforts on the devclopment of substitute crops to overcomethese differties. Government nowadays recommends the cultivation of the economiccrops like a flowers, medicinal herbs mushroom and clean vegetables. Medicinal herbsare specially profitable among these crops because herbs are possible to culture inwaste land, disused field and slope land, and owing to need less labor and competitionthan those of other crops. The most important problem is the facts that the compli-cation of currency structure of herb medicine inflicts mucll loss to cultivators. Therefore, this study was investigated the state of herb cultivation and the facts to be imploved in currency structure of the harvested herb medicine.1 . The cultivating area and output have been gradually increased and much produc-ted in Kyoungbuk, Kangwon, Choongbuk, Cheonbuk and Cheonnam province in or-der of cultivating area 2. Collection amount of wild herb medicine is decreasig bythe reason of the varous difficulties on the collection. 3. Cultivators of medicinal herbscan make agricultural management more resonable in information exchange on theherb cultivation, purchase of seed, fertilizer, chemicals and other materials, and sell-ing of harvested herb medicine by organization of cultivator fraternity. 4 Cultivatorshave to exclude intermediary margin by the development of direct transaction andcontract cultivation with medicinal herb store, drug manufacturer, chinese meicinehospital and trading firm etc. And also, by the performing exportation with foreign consumer.

• Journal of Veterinary Clinics
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• v.24 no.1
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• pp.5-9
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• 2007

The purpose of this study was to examine the allometric analysis of roxithromycin using pharmacokinetic data. The pharmacokinetic parameters used were $half-life(t_{1/2})$, mean residence time (MRT), clearance (Cl) and volume of distribution at steady state $(V_{ss})$. Relationships between body weight and the pharmacokinetic parameter were based on the empirical formula $Y=aW^b$, where 'Y' is $t_{1/2}$, MRT, Cl, or $V_{ss}$, W the body weight and 'a' is an allometric coefficient (intercept) that is constant for a given drug. The exponential term, 'b', is a proportionality constant that describes the relationship between the pharmacokinetic parameter of interest and body weight. As results of the allometric analyses, the logarithms of $t_{1/2}$, MRT, Cl, and $V_{ss}$ were linearly related to the logarithms of body weight. Results of the current analyses could provide information on appropriate doses of roxithromycin for all species.

• Journal of muscle and joint health
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• v.2 no.2
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• pp.185-196
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• 1995

The purposes of this study are to identify effectiveness of the hospital-based home care project, to manage patients' problems in comprehensive way, to decrease the patients' economical burden to the arthritis patients. The design is nonequivalent control group pretest-posttest design with matched samples in terms of age, sex and disease severity. Fifty two patients were assigned in each of the experimental and control groups in Seoul, Kyunggi, Kangwon and Kwangju. Before the experiment and after 3-month period of experiment of home care, level of pain, duration of morning stiffness, Richie Index, ADL, self-efficacy and depression were measured. Nine patients were excluded from the control group because of denial of participation. Contents of home care provided to the experimental group include distribution of prescribed drugs, assessment of patient's condition and side-reactions of drug, pain control, depression control, nutrition guide, exercise teaching, and family counselling. Patients assigned to the control group visited the outpatient clinic once a month as usual. Null hypotheses were selected because physicians concerned about the ineffective change of patients' conditions due to indirect communication with patients through nurses. Level of pain, Richie Index, ADL, self-efficacy, depression and duration of morning stiffness did not significantly different between two groups as expected. In the experimental group, level of pain, ADL, Richie Index and duration of morning stiffness changed to the positive direction from the pretest to the posttest. However, level of depression and self-efficacy did not show any changes. Ninety percent of patients in the experimental group satisfied with the home care provided to them. Since this is the intermediate report, more detailed and long-term report will be prepared.

• Journal of Ginseng Research
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• v.39 no.2
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• pp.141-147
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• 2015

Background: Adipocytes, which are the main cellular component of adipose tissue, are the building blocks of obesity. The nuclear hormone receptor $PPAR{\gamma}$ is a major regulator of adipocyte differentiation and development. Obesity, which is one of the most dangerous yet silent diseases of all time, is fast becoming a critical area of research focus. Methods: In this study, we initially aimed to investigate whether the ginsenoside Rf, a compound that is only present in Panax ginseng Meyer, interacts with $PPAR{\gamma}$ by molecular docking simulations. After we performed the docking simulation the result has been analyzed with several different software programs, including Discovery Studio, Pymol, Chimera, Ligplus, and Pose View. All of the programs identified the same mechanism of interaction between $PPAR{\gamma}$ and Rf, at the same active site. To determine the drug-like and biological activities of Rf, we calculate its absorption, distribution, metabolism, excretion, and toxic (ADMET) and prediction of activity spectra for substances (PASS) properties. Considering the results obtained from the computational investigations, the focus was on the in vitro experiments. Results: Because the docking simulations predicted the formation of structural bonds between Rf and $PPAR{\gamma}$, we also investigated whether any evidence for these bonds could be observed at the cellular level. These experiments revealed that Rf treatment of 3T3-L1 adipocytes downregulated the expression levels of $PPAR{\gamma}$ and perilipin, and also decreased the amount of lipid accumulated at different doses. Conclusion: The ginsenoside Rf appears to be promising compound that could prove useful in antiobesity treatments.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.95-95
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• 2018

Uric acid is the end product of purine metabolism in human body, originating from hypoxanthine after enzyme catalysis by Xanthine oxidase (XOD). Hyperuricemia results as a result of either over-generation of uric acid or a reduction in its excretion. In silico modelling methods such as Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) prediction, Autodock 4.2.6 program were used to study the potential inhibitory compounds of XOD. Also we investigated the inhibition of XOD activity by using the extracts of Dendropanax morbifera and Rubus coreanus Miq spectrophotometrically. According to ADMET data, several compounds from D. morbifera and R. coreanus plants, were found to be more potent in inhibiting the XOD activity than allopurinol. XOD inhibitory activity is evaluated by quantifying the formation of uric acid by measuring the absorbance at 290 m ($A_{290}$).D. morbifera extract inhibited XOD activity at $250{\mu}g/ml$, however the extracts from R. coreanus has inhibited XOD activity at $25{\mu}g/ml$. The major compound of R. coreanus, ellagic acid significantly increased the inhibition rate from $9{\mu}g/ml$ and showed a 71% suppression rate at $15{\mu}g/ml$. Finally, these results suggested a potential inhibitory activities of the extracts from D. morbifera and R. coreanus Miq, but further research is needed to validate to ensure their safe usage as drug.

• Archives of Pharmacal Research
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• v.27 no.6
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• pp.653-661
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• 2004

Fluorescence probes located in different membrane regions were used to evaluate the effect of dopamine$.$HCI on the structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and thickness of the lipid bilayer) of synaptosomal plasma membrane vesicles (SPMV), which were obtained from the bovine cerebral cortex. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, and radiationless energy transfer from the tryptophan of membrane pro-teins to Py-3-Py and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS) was also utilized. Dopamine$.$HCI increased both the bulk lateral mobility and annular lipid fluidity, and it had a greater fluidizing effect on the inner monolayer than on the outer monolayer. Furthermore, the drug had a clustering effect on membrane proteins.

• YAKHAK HOEJI
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• v.51 no.6
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• pp.469-475
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• 2007

The aim of this study is to find problems and possible improvement for current continuing education (CE) of pharmacists by assessing present status and performing survey data analysis. Present CE for pharmacists is administered by Korean Pharmaceutical Association and it has three separate modules for pharmacists depending on their specialty. The modules for community pharmacy and hospital pharmacy are mainly focused on patient care while the module for industrial pharmacists is on manufacturing and distribution of drugs. Survey data analysis revealed that more than half of the responders feel that present CE is inappropriate in terms of length and contents of the CE. Furthermore, about 40% of all responders answered it does not help them update professional knowledge. With regard to possible improvement of CE, 86% of the responders felt an accreditation council of CE needs to be established, and 64% answered that present three separate modules should be unified. More than three quarter (79%) of the responders wanted to have some part of CE administered by correspondence instead of on-site CE. Based on the findings from this study, present CE for pharmacists appears to need improvement to ensure appropriate drug use and to meet expectations of pharmacists who see problems on the present CE.