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Effects of Dopamine.HCI on Structural Parameters of Bovine Brain Membranes  

Bae, Moon-Kyoung (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Huh, Min-Hoi (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Lee, Seung-Woo (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Kang, Hyun-Gu (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Pyun, Jae-Ho (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Kwak, Myeong-Hee (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Jang, Hye-Ock (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Yun, Il (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Publication Information
Archives of Pharmacal Research / v.27, no.6, 2004 , pp. 653-661 More about this Journal
Abstract
Fluorescence probes located in different membrane regions were used to evaluate the effect of dopamine$.$HCI on the structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and thickness of the lipid bilayer) of synaptosomal plasma membrane vesicles (SPMV), which were obtained from the bovine cerebral cortex. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, and radiationless energy transfer from the tryptophan of membrane pro-teins to Py-3-Py and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS) was also utilized. Dopamine$.$HCI increased both the bulk lateral mobility and annular lipid fluidity, and it had a greater fluidizing effect on the inner monolayer than on the outer monolayer. Furthermore, the drug had a clustering effect on membrane proteins.
Keywords
Dopamine·HCI; Transbilayer lateral mobility; Membrane thickness; Annular lipid fluidity; Membrane protein clustering;
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