• Molecules and Cells
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• v.26 no.6
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• pp.595-605
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• 2008

Genome wide transcription analysis in response to stresses is essential to provide the basis of effective engineering strategies to improve stress tolerance in crop plants. In order to perform transcriptome analysis in Brassica rapa, we constructed a B. rapa oligo microarray, KBGP-24K, using sequence information from approximately 24,000 unigenes and analyzed cold ($4^{\circ}C$), salt (250 mM NaCl), and drought (air-dry) treated B. rapa plants. Among the B. rapa unigenes represented on the microarray, 417 (1.7%), 202 (0.8%), and 738 (3.1%) were identified as responsive genes that were differently expressed 5-fold or more at least once during a 48-h treatment with cold, salt, and drought, respectively. These results were confirmed by RT-PCR analysis. In the abiotic stress responsive genes identified, we found 56 transcription factor genes and 60 commonly responsive genes. It suggests that various transcriptional regulatory mechanisms and common signaling pathway are working together under the abiotic stresses in B. rapa. In conclusion, our new developed 24K oligo microarray will be a useful tool for transcriptome profiling and this work will provide valuable insight in the response to abiotic stress in B. rapa.

• Journal of Life Science
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• v.19 no.10
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• pp.1456-1462
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• 2009

D-chiro-Inositol, which is the isomer of myo-inositol, is a well known drug for the treatment of type II diabetes. The methylated form of D-chiro-inositol, D-pinitol and D-chiro-inositol are synthesized when the plants are exposed to the abiotic stresses such as drought, salinity and low temperature as osmoprotectants. In soybean, myo-inositol is converted to ononitol by O-methyltransferase, and ononitol is converted to D-pinitol by ononitol epimerase and finally converted to D-chiro-inositol by demethylase. However there have been some reports that in buckwheat, myo-inositol can be converted to D-chiro-inositol directly. This study was conducted to determine the changes of soluble cyclitols in buckwheat seedlings after exposure to salt and drought stresses by GC-FID. The results indicated that myo-inositol may be the precursor of D-chiro-inositol biosynthesis.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.19-19
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• 2019

It is necessary to maintain stable crop productions under the unsuitable environments, because the drought and flood may be frequently caused by the global warming. Therefore, it is agent to improve the crop growth model corresponded to soil moisture status. Chili pepper (Capsicum annuum) is one of the useful crop in Asia, and then it is affected by change of precipitation in consequence drought and flood occur however crop model to evaluate water stresses on chili pepper is not enough yet. In this study, development of crop model under different soil moisture status was attempted. The experiment was conducted on the slope fields in the greenhouse. The water level was kept at 20cm above the bottom of the container. Habanero (C. chinense) was used as material for crop model. Sap bleeding rate, SPAD value, chlorophyll content, stomatal conductance, leaf water potential, plant height, leaf area and shoot dry weight were measured at 10 days after treatment (DAT) and 13 DAT. Moreover, temperature and RH in the greenhouse, soil volume water contents (VWC) and soil water potential were measured. As a result, VWC showed 4.0% at the driest plot and 31.4% at the wettest plot at 13 DAT. The growth model was calculated using WVC and the growth analysis parameters. It was considered available, because its coefficient of determination showed 0.84 and there are significant relationship based on plants physiology among the parameters and the changes over time. Furthermore, we analyzed the important factors for higher accuracy prediction using multiple regression analysis.

• Journal of Korean Society of Water and Wastewater
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• v.35 no.6
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• pp.477-487
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• 2021

Due to the global climate change, Korean peninsula is has been experiencing flooding and drought severely. It is hard difficult to manage water resources sustainably, because due to intensive precipitation in short periods and severe drought has increased in Korea. Reused water from the wastewater treatment plant (WWTP) could be a sustainable and an alternative water source near the urban areas. In order to understand the patterns of water reuse in Korea, annual water reuses data according to the times and regional governments were investigated from 2008 to 2019. The reused water from WWTP in Korea has been mainly used for river maintenance flow and industrial use, while agricultural use of water reuse has decreased with time. Metropolitan cities in Korea such as Seoul, Busan, Daegu, Ulsan, and Incheon have been mainly used reused reusing water for river maintenance flow. Industrial water reuse has been limitedly applied recently for the planned industrial districts in Pohang, Gumi, Paju, and Asan. By using the collected annual water reuse data from the domestic sewerage statistics of sewerage, the optimistic and pessimistic future estimations of for future annual water reuse were suggested from 2020 to 2040 on a five year interval for every five years.

• Applied Biological Chemistry
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• v.42 no.3
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• pp.246-251
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• 1999

This experiment was carried out to elucidate and investigate the changes in the content of antioxidants and the activities of antioxidant enzymes in the leaves of soybean subjected to water stresses. The results obtained were as follows; Leaves of soybeans subjected to water stresses have showed the differences in the activities of the antioxidant enzymes. In eunhakong, the activity of APOX was increased within a few days, but that of GR was decreased, whereas the activities of APOX and GR were gradually decreased in eunhakong. The activity of MDHAR of the leaves of eunhakong subjected to drought stress was gradually increased within 4days, whereas that of flooding was increased within 2days. We are supposed that the activities of APOX and MDHAR are coupled to maintain ascorbate concentration. In eunhakong, the relative activity of DHAR subjected to flooding was higher than that of drought. These results imply that DHAR is the only enzyme participating in the regeneration of ascorbate when the activity of MDHAR was limited by the deficiency of NADPH. The contents of ascorbate and reduced glutathione subjected to drought stress decreased continually, whereas those subjected to flooding stress recovered after five days of treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.2
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• pp.148-153
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• 2004

The Hessian fly, Mayetiola destructor (Say), is known to be one of the major insect herbivores of wheat worldwide. In order to provide molecular events on interactions of the NIL with H21 and larvae of Hessian fly biotype L, the TaCR1 gene, Triticum aestivum cytokinin repressed 1, was isolated through the suppression subtractive hybridization, which was constructed using stems of the NIL with H21 at 6 days after infestation as tester and stems of the recurrent parent Coker797 without H21 at 6 days after infestation as driver. Transcript levels of TaCR1 mRNA in the NIL with H21 were highest at 6 days after infestation but in the Coker797 without H21 until 8 days were similar with those of non-infested plants. Expression of the TaCR1 gene was decreased at early time and then recovered after wounding or $H_2O$$_2$ treatment as well as 6-BAP treatment. Transcripts levels of the TaCR1 gene was changed after MeJA, SA, ethephone, or ABA treatment. In drought treatment, the TaCRl gene were increased at early stage of stress and then decreased at late stage. Expression of the TaCRl gene was continued to decrease through 24 h in the cold treatment. Although the TaCRl gene is increased through infestation in NIL with H21, further study was required to elucidate a role on resistance against larvae of Hessian fly. However, the TaCR1 gene could be used as marker gene on response of plants against abiotic stresses as well as application of plants with several hormones.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.85-90
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• 1991

Weaner heifers were set stocked at 4/ha on a grass-legume pasture in South Sulawesi, Indonesia, and either unsupplemented (Control) or for 338 days given daily supplements of one of, rice bran (RB) supplied at 1 kg/animal/d, molasses/urea (MU) or 0.5 RB + 0.5 MU (MURB) the amounts of which were adjusted to give similar energy intakes. There were 20 animals in each treatment. A drought resulted in low pasture availability for about half the supplementation period. The LWG per animal in the MURB treatment was 85 kg above that of the control and this was significantly greater (p < 0.01) than those for MU (62.0 kg) or RB (56.2 kg) although the economics favoured the gains from RB which returned over three times the cost of the supplement. Costs could be reduced by supplementing only at times of maximum undernutrition, but such a strategy is of doubtful value in this situation as there was no compensatory LWG and a similar rate of economic return was maintained throughout the period. The results suggest that additional benefits from the supplementation may be improved reproductive performance and more efficient use of pasture.

• Korean Journal of Plant Resources
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• v.21 no.3
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• pp.204-209
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• 2008

We have cloned an LTP gene (PoLTP1) from poplar (Populus alba ${\times}$ P. tremula var. glandulosa) suspension cells and examined changes in its expression levels in response to various stresses and ABA treatment. The full-length PoLTP1 cDNA clone encodes a polypeptide of 116 amino acids with typical characteristics of LTPs, notably a conserved arrangement of cysteine residues. Southern blot analysis indicate that two or three copies of the PoLTP1 are present in the genome of the investigated hybrid poplar. In addition, northern analysis of samples from soil-grown plants indicate that PoLTP1 is tissue-specifically expressed in the leaves and flowers. The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding. These results indicate that PoLTP1 is involved in osmotic stress responses in poplar plants and suspension cells.

• BMB Reports
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• v.42 no.9
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• pp.611-616
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• 2009

We investigated the expression pattern of dehydration responsive element-binding protein-3 in tomato under different abiotic stresses. Full length LeDREB3 cDNA was isolated from tomato plant, followed by phylogenetic analysis based on deduced amino acid sequences that revealed significant sequence similarity to DREB proteins belonging to diverse families of plant species. Southern blot analysis showed duplicate copies of LeDREB3 in the tomato genome while organ-specific expression profiling indicated constitutive expression of LeDREB3 in all tested organs, which was particularly strong in flower. LeDREB3 expression was significantly induced by Nacl, drought, low temperature and $H_2O_2$. Moreover, LeDREB3 was slightly regulated by treatment with ABA and MV. These observations suggest that the LeDREB3 gene may be involved in the response of the tomato plant to stress.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.1
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• pp.54-61
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• 2000

Covering rate, visual rating and sod development were evaluated under three natural fiber materials with Phragmites spp. when over a plastic sheet. The results were as follows. (1) The last covering rate was high on jute net, coir mat and on Miscanthus sacchariflorus, respectively while the early covering rate was high on coir mat and on Miscanthus sinensis+perennial ryegrass. (2) The early growth was good on perennial ryegrass but the covering rate gradually turned poor because of summer drought. (3) Sod was highly developed on Phragmites japonica, Miscanthus sacchariflorus and Miscanthus sinensis compared with other species and mixtures. (4) The covering rate and visual rating were high on natural fiber materials such as coir mat and jute net when compared with on natural fiber materials such as none treatment plots. (5) The natural fibers materials on Phragmites spp. and Miscanthus spp. were effect on sod establishment. Sod coir mat was highly established. (6) The carpet-type sod was best developed on the coir mat.