KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Expression of the TaCR1 Gene Induced by Hessian Fly Larval Infestation in Wheat Carrying a H21 Gene.

  • Jang, Cheol-Seong (Laboratory of Plant Molecular Breeding, Division of Biotechnology and Genetic Engineering, College of Life and Environmental Science, Korea University) ;
  • Seo, Yong-Weon (Laboratory of Plant Molecular Breeding, Division of Biotechnology and Genetic Engineering, College of Life and Environmental Science, Korea University)
  • Published : 2004.06.01
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Abstract

The Hessian fly, Mayetiola destructor (Say), is known to be one of the major insect herbivores of wheat worldwide. In order to provide molecular events on interactions of the NIL with H21 and larvae of Hessian fly biotype L, the TaCR1 gene, Triticum aestivum cytokinin repressed 1, was isolated through the suppression subtractive hybridization, which was constructed using stems of the NIL with H21 at 6 days after infestation as tester and stems of the recurrent parent Coker797 without H21 at 6 days after infestation as driver. Transcript levels of TaCR1 mRNA in the NIL with H21 were highest at 6 days after infestation but in the Coker797 without H21 until 8 days were similar with those of non-infested plants. Expression of the TaCR1 gene was decreased at early time and then recovered after wounding or $H_2O$$_2$ treatment as well as 6-BAP treatment. Transcripts levels of the TaCR1 gene was changed after MeJA, SA, ethephone, or ABA treatment. In drought treatment, the TaCRl gene were increased at early stage of stress and then decreased at late stage. Expression of the TaCRl gene was continued to decrease through 24 h in the cold treatment. Although the TaCRl gene is increased through infestation in NIL with H21, further study was required to elucidate a role on resistance against larvae of Hessian fly. However, the TaCR1 gene could be used as marker gene on response of plants against abiotic stresses as well as application of plants with several hormones.

Keywords

References

  1. Chandler, P. M. and M. Robertson. 1994. Gene expression regulated by abscissic acid and its relation to stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. BioI. 45: 113-141 https://doi.org/10.1146/annurev.pp.45.060194.000553
  2. Chen, C., J. R. Erti, S. M. Leisner, and C. Chang. 1985. Localization of cytokinin biosynthesis site in pea plants and carrot roots. Plant Physiol. 78:510-513 https://doi.org/10.1104/pp.78.3.510
  3. Grover, P. B., D. R. Ross, and R. H. Shukle. 1988. Identification and partial charaterization of digestive carbohydrases in larvae of the Hessianfly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae). Arch. Insect Biochem. PhysioI. 5:59-72
  4. Hermsmeier, D., U. Schittko, and I. T. Baldwin. 2001. Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera Sphingidae) and its natural host Nicotiana attenuata. I. Large-scale changes in the accumulation of growth- and defense- related plant mRNAs. Plant PhysioI. 125: 683-700 https://doi.org/10.1104/pp.125.2.683
  5. Jang, C. S., B. H. Hong, and Y. W. Seo. 1999. Expressed sequence tags of the wheat-rye translocation line possessing 2RL. Korean J. Crop Sci. 44: 302-307
  6. Jang, C. S., D. S. Kim, S. Y. Bu, J. B. Kim,S. S.Lee, J. Y. Kim, J.W. Johnson, and Y. W. Seo. 2002. Isolation and characterization of lipid transfer protein (LTP) genes from a wheat-rye translocation line. Plant Cell Rep. 20: 961-966 https://doi.org/10.1007/s00299-001-0424-x
  7. Jang, C. S., J. Y. Kim, J. W. Haam, M. S. Lee, D. S. Kim, Y. W. Li, and Y. W. Seo. 2003a. Expression sequences tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]. Plant Cell Rep. 22: 150-158 https://doi.org/10.1007/s00299-003-0641-6
  8. Jang, C. S., M. S. Lee, J. Y. Kim, D. S. Kim, and Y. W. Seo. 2003b, Molecular haracterization of a cDNA encoding putative calcium binding protein, HvCaBP1, induced during kernel development in barley (Hordeum vulgare L.). Plant Cell Rep. 22: 64-70 https://doi.org/10.1007/s00299-003-0665-y
  9. Kunkel, B. N. and D. M. Brooks. 2002. Cross talk between signaling pathways in pathogen defense. Curro Opin. Plant Biol, 5: 325-331 https://doi.org/10.1016/S1369-5266(02)00275-3
  10. L, L., C. Li, G. I. Lee, and G. A. Howe. 2002. Distinct roles for jasmonate synthesis and action in the systemic wound response of tomato. Proc. Natl. Acad. Sci. USA 99: 6416-6421 https://doi.org/10.1073/pnas.072072599
  11. Refai, F. Y, E. T. Jones, and B. S. Miller. 1955. Some biological factors involved in the resistance of the wheat plant to attack by the Hessian fly. Cereal Chem. 32: 437-451
  12. Senaratna, T., D. Touchell, E. Bunn, and K. Dixon. 2000. Acety1 salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul. 30: 157-161 https://doi.org/10.1023/A:1006386800974
  13. Seo, Y. W., C. S. Jang, and J. W. Johnson. 2001. Development of AFLP and STS markers for identifying wheat-rye translocations possessing 2RL. Euphytica 121: 279-287 https://doi.org/10.1023/A:1012020532485
  14. Seo, Y. W., J. W. Johnson, and R. L. Jarret. 1997. A molecular marker associated with the H21 Hessian fly resistance gene in wheat. Mol. Breed. 3: 177-181 https://doi.org/10.1023/A:1009606304447
  15. Teramoto, H., E. Momotani, G. Takeba, and H. Tsuji. 1993. Isolation of a cDNA clone for a cytokinin-repressed gene in excised cucumber cotyledons. Planta 193: 573-579 https://doi.org/10.1007/BF02411564
  16. Teramoto, H., T. Toyama, G. Takeba, and H. Tsuji. 1995. Changes in expression of cytokinin-repressed genes, CR9 and CR20 in relation to aging, greening and wounding in cucumber. Planta 1996: 387-395
  17. van de Ven, W. G., C. S. LeVesque, T. M. Perring, and L. L. Walling. 2000. Local and systemic changes in squash gene expression in response to silverleaf whitefly feeding. Plant Cell 12: 1409-1423 https://doi.org/10.1105/tpc.12.8.1409
  18. WilIams, C. E., C. C. Collier, J. A. Nemacheck, C. Liang, and S. E, Cambron. 2002. A lectin-like wheat gene responds systemically to attempted feeding by avirulent first-instar Hessian fly larvae. J. Chern. Ecol. 28:1411-1428 https://doi.org/10.1023/A:1016200619766