• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.390-393
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• 2001

The optimal temperature, pH and aeration rate for spore production by Bacillus polyfermenticus SCD in 500 ml shake flask and 5-1 jar fermenter were found to be $32^{\circ}C$, 7.0 and 1.0 vvm. respectively. When batch culture processes was performed under optimized culture conditions. viable cells were $3.3{\times}10^{10}$ CFU/ml and spore cells were $3.3{\times}10^{10}$ CFU/ml. Fed-batch culture processes were also examined with regard to higer maximum viable cell and spore production. The highe viable cells and spores were obtained in 5-1 jar fermenter at 72 h cultivation time by strategy in an intermediate feeding mode with 60% glucose solution 150 ml and 5% soybean flour solution 150 ml fed to the fermenter twice, and the productivity of spore cells was significantly increased. Finally. volumetric productivity of spore cells on fed-batch culture indicated $9.9{\times}10^8$ CFU/ml/h, which was approximately 2 times higher than batch culture. Thus, fed-batch culture show a promise as an industrial production method.

• 중환자간호학회지
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• 제6권1호
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• pp.44-56
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• 2013

Purpose: The purpose of this study was to investigate emergency room nurses' recognition of patient safety culture and their performance of safety management activity. Methods: Data were collected from July 1 to August 31, 2012 on 292 emergency room nurses working at 25 general hospitals located in B city in G province. The Hospital Survey on Patient Safety Culture was used to measure patient safety culture, and an 82-item questionnaire was developed to measure safety management activity. Results: the performance of safety management activity were significantly associated with the total career years, whether the nurses had undergone safety training, and whether the nurses has been working in the regional emergency care facility. Of 6 subcategories of the patient safety culture, the perception of a directly commanding senior/manager, frequency of accident reports, and hospital environment were associated with the performance of safety management activity. Conclusion: For improving performance of safety management activity among emergency room nurses, it is necessary to develop an educational program of safety management activity by their level of performance.

• 생명과학회지
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• 제26권1호
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• pp.68-74
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• 2016

퍼클로레이트(ClO₄⁻)는 지표수 및 토양/지하수에서 검출되는 신규 오염물이다. 이전 연구에서 저렴한 원소 황(elemental sulfur, S⁰) 입자와 쉽게 구할 수 있는 활성슬러지를 이용하여 독립영양방식으로 ClO₄⁻를 제거할 수 있다는 실험적 증거가 제시되었다. 또한 식종균으로서 농화배양 미생물을 사용했을 때 활성슬러지보다 제거효율과 시간면에서 우수한 결과를 나타내었다. 그래서 본 연구에서는 황을 산화하여 독립영양방식으로 ClO₄⁻를 분해하는 농화배양 미생물 군집을 PCR-DGGE로 분석하였다. 이 농화배양 미생물은 초기농도가 약 120 mg ClO₄⁻/l 일 때 7일 후 99.71% 이상의 ClO₄^- 제거 효율을 나타내었다. 농화배양 미생물과 그것의 식종균으로 부터 genomic DNA를 추출하여 16S rRNA 유전자의 PCR-DGGE 분석에 사용하였다. PCR-DGGE 분석결과 농화배양 미생물과 식종균 시료들이 다른 밴드패턴을 나타냄에 따라 이 두 시료의 군집조성이 다름을 확인하였다. 이는 농화배양되는 동안 식종된 미생물이 그 환경에 잘 생장하는 미생물로 군집조성이 변화한 것으로 여겨진다. 농화배양 미생물군집에는 β-Proteobacteria, Bacteroidetes, 그리고 Spirochaetes 강에 속하는 개체군들이 우점하는 것으로 나타났다. 향후 이 우점 개체군들의 순수분리와 더불어 황 산화를 통한 ClO₄⁻ 분해 환경에서 이들의 대사적 역할을 규명할 필요가 있다.

• Journal of Plant Biotechnology
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• 제7권3호
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• pp.169-174
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• 2005

The optimal culture conditions were studied for plant regeneration from mesophyll protoplasts of Solanum sisymbriifolium. Axenic seedlings of S. sisymbriifolium were used as a explant for protoplast culture. Many viable protoplasts were isolated by incubating leaf slices in an enzyme solution containing 0.25% Meicerase and 0.05% Macerozyme for 16 hr at $25^{\circ}C$ without shaking. Protoplast density of $5.0{\times}10^4\;ml^{-1}$ in Kao medium containing 5.0 mg/L NAA, 1.0 mg/L 2,4-D and 1.0 mg/L BA was optimal for colony formation. Most colonies were formed when protoplasts were cultured at $25^{\circ}C$ after initial culture at $30^{\circ}C$ for one week. On the MS agar medium with 1.0 mg/L zeatin, 38.4% of protoplast-derived calli differentiated shoots. These shoots rooted on 1/2MS medium with 5.0 g/L sucrose and 2.5 g/L gellan gum, and developed into whole plants.

• 패션비즈니스
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• 제13권6호
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• pp.1-11
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• 2009

Dong Tribe is a populous nationality with brilliant civilization, they created their unique culture of costumes and accessories throughout the long river of history. And the children's costumes could be the florid feature of the splendid fashion of Dong Tribe because they reflect the sentimental tastes and consciousness of the nation. The children's costumes of Dong Tribe are classified as infants' garments and children's garibaldis by different ages, the garments are much more in kinds, fresher in colors and much complicated in shapes while comparing with the adults' garments. Furthermore, the children's hats, bibs and baby carriers are also the outstanding features in children's costumes of Dong Tribe.

• 한국디지털건축인테리어학회논문집
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• 제13권4호
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• pp.51-60
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• 2013

With regard to urban culture this study aims to essential understanding and systematic approach to the culture. The "2012 Seoul Survey" report has been used to find out causality among the related variables. In the first place 'satisfaction of cultural condition' was operationally selected as a dependent variable for regression. For the purpose of controlling the third factors for ceteris paribus effect correlation analysis was previously done between the dependent variable and all other variables respectively, which resulted in two groups of variables: group (1) - 2 variables of very significant correlations(p-value<0.01) and (2) - the other 6 variables of significant correlations(p-value<0.05). Then hierarchical regression was adopted to these 2 groups to analyse statistical significance of independent variables, and multicollinearity(VIF; variance inflation factor). Additionally to OLS robust and bootstrapping regressions were done to confirm the validity of this model specification. At last a regression model specified by group (1) as independent variables(they are 'community spirit caring for women, the disabled, the poor and the old,' 'satisfaction of bicycle riding condition' shows that the variables have statistically significant and substantially strong effect on 'satisfaction of cultural condition.' This finding implies the following understanding; (1) urban festivals are regarded as the main of the urban culture as of now and this results from the low level of today's culture, (2) culture is telling and hearing stories but the influential relationship between urban culture and community spirit on the weak is negative, which says that the cultural perception among citizen is somewhat selfish and far from the essential understanding of the urban culture. In spite of restrictive external validity this finding can be used as a direction for promoting culture and a basis for related policy choice in cities.

• International Journal of Advanced Culture Technology
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• 제10권4호
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• pp.536-546
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• 2022

A society will lose a lot of something in this field when the forest fire broke out. If a forest fire can be detected in advance, damage caused by the spread of forest fires can be prevented early. So, we studied how to detect forest fires using CCTV currently installed. In this paper, we present a deep learning-based model through efficient image data construction for monitoring forest fire smoke, which is unstructured data, based on the deep learning model YOLOv5. Through this study, we conducted a study to accurately detect forest fire smoke, one of the amorphous objects of various forms, in YOLOv5. In this paper, we introduce a method of self-learning by producing insufficient data on its own to increase accuracy for unstructured object recognition. The method presented in this paper constructs a dataset with a fixed labelling position for images containing objects that can be extracted from the original image, through the original image and a model that learned from it. In addition, by training the deep learning model, the performance(mAP) was improved, and the errors occurred by detecting objects other than the learning object were reduced, compared to the model in which only the original image was learned.

• 복식문화연구
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• 제16권4호
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• pp.696-708
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• 2008

The purpose of this study was to examine the relationship among Shopping Orientation, Shopping-mall characteristics, Consumer Satisfaction, and Loyalty in online market. Data were collected from 405 adult women and analyzed by factor analysis, cluster analysis, ANOVA and Duncan test using by SPSS 12.0. The results showed that recreation shoppers to buy the cosmetics in online market, were more likely to perceive the shopping mall characteristics, information, check-out convenience, and product price of shopping mall than the other shoppers. Both brand oriented shoppers and economic oriented shoppers were more likely to perceive information offered at newsletters and e-mail of Internet shopping mall. Additionally, the more active shoppers were the more satisfied and the greater loyalty at online shopping mall of cosmetics. Implications are drawn for the information useful marketer and retailers of the online shopping mall of cosmetics.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.216-219
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• 2004

The effect of culture temperature on the production of soluble form of B. macerans cyclodextrin glucanotransferase (CGTase) in recombinant E. coli was investigated. E. coli cell was cotransformed with two plasmids (pTCGT1 and pGroll) in which the cgt and groEL/ES genes are under the control of T7 promoter and pzt-1 promoter, respectively. When tetracycline (10 ng/ml) and IPTG (l mM) were added as inducers at the early-exponential phase (2 h) and mid-exponential phase (3h), respectively, the solubilization of the inclusion body CGTase was greatly dependent on the temperature of the culture. At low culture temperature of $25^\circ{C}$, 2- or 3-fold higher activity and specific activity were obtained over $37^\circ{C}$. SDS-PAGE analysis revealed that about 62% of CGTase in the total CGTase protein was found in the soluble fraction by applying overexpression of GroEL/ES chaperone and by cultivation of E. coli at $25^\circ{C}$, whereas 33% of CGTase was detected in the soluble fraction at $37^\circ{C}$. Therefore, the expression of GroEL/ES and cultivation at $25^\circ{C}$ greatly enhanced the soluble production of CGTase in E. coli.

• 한국의상디자인학회지
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• 제22권1호
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• pp.45-59
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• 2020

In the 1950s, Korea was forced to rely on foreign aid, relief supplies, and contraband because of the Korean War. At the same time, Western culture also flowed into the country rapidly, which is believed to have had a great impact on the Korean clothing culture of the time. It is thought that this trend could be found in magazines and newspapers of the time. In the 1950s, newspapers served as the main channel for information acquisition and dissemination. I would like to analyze this tendency focusing on a newspaper article in the Dong-A Ilbo in the 1950s. According to the article, the hanbok was viewed as an old custom that needed to be improved or old clothes that had to be changed to Western clothes. As a result, the doubles system of both hanbok and Western clothes collapsed, and hanbok changed to an evening or ceremonial dress. From the 1960s, Korean fashion quickly changed to a more western style.