• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1701-1707
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• 2016

Lipoxygenase (LOX) is an industrial enzyme with wide applications in food and pharmaceutical industries. The available structure information indicates that eukaryotic LOXs consist of N terminus β-barrel and C terminus catalytic domains. However, the latest crystal structure of Pseudomonas aeruginosa LOX shows it is significantly different from those of eukaryotic LOXs, including the N-terminal helix domain. In this paper, the functions of this N-terminal helix domain in the soluble expression and catalysis of P. aeruginosa LOX were analyzed. Genetic truncation of this helix domain resulted in an insoluble P. aeruginosa LOX mutant. The active C-terminal domain was obtained by dispase digestion of the P. aeruginosa LOX derivative containing the genetically introduced dispase recognition sites. This functional C-terminal domain showed raised substrate affinity but reduced catalytic activity and thermostability. Crystal structure analyses demonstrate that the broken polar contacts connecting the two domains and the exposed hydrophobic substrate binding pocket may contribute to the insoluble expression of the C terminus domain and the changes in the enzyme properties. Our data suggest that the N terminus domain of P. aeruginosa LOX is required for its soluble expression in E. coli, which is different from that of the eukaryotic LOXs. Besides this, this N-terminal domain is not necessary for catalysis but shows positive effects on the enzyme properties. The results presented here provide new and valuable information on the functions of the N terminus helix domain of P. aeruginosa LOX and further improvement of its enzyme properties by molecular modification.

• Proceedings of the KIEE Conference
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• 2005.10b
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• pp.539-541
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• 2005

We propose efficient video retrieval scheme which use fast key frame extraction in DCT domain. Our scheme extract key frame using the edge histogram difference which is extracted in compressed domain for I-frames. And the video retrieval is implemented using Hausdorff distance function about edge histogram of key frame. This approach enables fast content-based video retrieval of the compressed video content without decompression process. Experimental results show our scheme is very fast and efficient.

• Proceedings of the KIEE Conference
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• 2005.10b
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• pp.536-538
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• 2005

As multimedia data and huge-Quantity video data having been increasingly and commonly used, the key frame algorithm, as one of the methods for manipulating these kinds of data, became an important matter and has been studied for many years. But the formerly proposed key frame extraction methods take much processing time or need complex calculations due to decoding processes. In order to solve these problems which the former methods have and to enhance the key frame extraction efficiency, a novel key frame extraction method in compressed domain is proposed in this paper. In this method we get an edge histogram for each I-frame in DCT domain and then extract the key frames by means of histogram difference metric. Experimental results show that our algorithm achieves fast processing speed and high accuracy.

• Geomechanics and Engineering
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• v.34 no.1
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• pp.1-15
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• 2023

This study conducts uniaxial compression tests on intact and single crack-contained rocks to investigate the time-frequency domain characteristics of acoustic emission (AE) signals monitored during the deformation failure process. A processing approach, short-time Fourier transform (STFT), is performed to obtain the evolution characteristics of time-frequency domain of AE signals. The AE signal modes at different deformation stages of rocks are different. Five modes of AE signal are observed during the cracking process of rocks. The evolution characteristics of time-frequency domain of AE signals processed by STFT can be utilized to evaluate the damage process of rocks. The difference of time-frequency domain characteristics between intact and cracked rocks is comparatively analyzed. The distribution characteristics of frequency changing from a single band-shaped cluster to multiple band-shaped clusters can be regarded as an early warning information of damage and failure of rocks. Meanwhile, the attenuation of frequency enables the exploration of rock failure trends.

• BMB Reports
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• v.40 no.1
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• pp.44-52
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• 2007

Kinesins, as a kind of microtubule-based motor proteins, have a conserved microtubule-binding site in their motor domain. Here we report that two homologous kinesins in Arabidopsis thaliana, KatB and KatC, contain a second microtubule-binding site in their tail domains. The prokaryotic-expressed N-terminal tail domain of the KatC heavy chain can bind to microtubules in an ATP-insensitive manner. To identify the precise region responsible for the binding, a serious of truncated KatC cDNAs encoding KatC N-terminal regions in different lengths, KatC1-128, KatC1-86, KatC1-73 and KatC1-63, fused to Histidine-tags, were expressed in E. coli and affinity-purified. Microtubule cosedimentation assays show that the site at amino acid residues 74-86 in KatC is important for microtubule-binding. By similarity, we obtained three different lengths of KatB N-terminal regions, KatB1-384, KatB1-77, and KatB1-63, and analyzed their microtubule-binding ability. Cosedimentation assays indicate that the KatB tail domain can also bind to microtubules at the same site as and in a similar manner to KatC. Fluorescence microscopic observations show that the microtubule-binding site at the tail domain of KatB or KatC can induce microtubules bundling only when the stalk domain is present. Through pull-down assays, we show that KatB1-385 and KatC1-394 are able to interact specifically with themselves and with each other in vitro. These findings are significant for identifying a previously uncharacterized microtubule-binding site in the two kinesin proteins, KatB and KatC, and the functional relations between them.

• Wind and Structures
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• v.28 no.3
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• pp.171-180
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• 2019

This paper presents a restart iterative approach for time-domain flutter analysis of long-span bridges using the commercial FE package ANSYS. This approach utilizes the recursive formats of impulse-response-function expressions for bridge's aeroelastic forces. Nonlinear dynamic equilibrium equations are iteratively solved by using the restart technique in ANSYS, which enable the equilibrium state of system to get back to last moment absolutely during iterations. The condition for the onset of flutter instability becomes that, at a certain wind velocity, the amplitude of vibration is invariant with time. A long-span suspension bridge was taken as a numerical example to verify the applicability and accuracy of the proposed method by comparing calculated results with wind tunnel tests. The proposed method enables the bridge designers and engineering practitioners to carry out time-domain flutter analysis of bridges in commercial FE package ANSYS.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.13 no.4
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• pp.109-127
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• 2003

In this paper, we analyze the network architecture, authentication, and key agreement of GSM and UMTS to compare the handover between the systems. And then, we divide authentication and key agreement procedure of mobile subscribers into several cases and finally analyze the key agreement procedure when a handover occurs in a CS-Domain and a PS-Domain.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1227-1233
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• 2015

Vascular endothelial growth factor (VEGF) plays a key role in angiogenesis through binding to its specific receptors, which mainly occurs to VEGF receptor 2 (VEGFR-2), a kinase insert domain-containing receptor. Therefore, the disruption of VEGFR-2 signaling provides a promising therapeutic approach for the treatment of cancer by inhibiting abnormal or tumorinduced angiogenesis. To explore this potential, we expressed the catalytic domain of VEGFR-2 (VEGFR-2-CD) as a soluble active kinase in Escherichia coli. The recombinant protein was purified and the VEGFR-2-CD activity was investigated. The obtained VEGFR-2-CD showed autophosphorylation activity and phosphate transfer activity comparable to the commercial enzyme. Furthermore, the IC₅₀ value of known VEGFR-2 inhibitor was determined using the purified VEGFR-2-CD. These results indicated a possibility for functional and economical VEGFR-2-CD expression in E. coli to use for inhibitor screening.

• Steel and Composite Structures
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• v.46 no.3
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• pp.435-449
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• 2023

Time domain method and frequency domain method are commonly used in the current fatigue life calculation theory. The time domain method has complicated procedures and needs a large amount of calculation, while the frequency domain method has poor applicability to different materials and different spectrum, and improper selection of spectrum model will lead to large errors. Considering that artificial neural network has strong ability of nonlinear mapping and generalization, this paper applied this technique to random fatigue life prediction, and the effect of average stress was taken into account, thereby achieving more accurate prediction result of random fatigue life.

• Proceedings of the IEEK Conference
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• 2002.06d
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• pp.85-88
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• 2002

In this paper, the proposed watermark algorithm is based on energy distribution of the subband coefficients in the frequency domain and edge of the original image in the spacial domain. Out of these information, the KeyMap which decides the embedded position of watermark is produced. And then the binary watermark is embedded into the wavelet coefficient of LL3 subband using KeyMap and LFSR(Linear Feedback Shift Register).