• BMB Reports
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• v.46 no.1
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• pp.37-40
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• 2013

CY-007 and CY-049 pteridine glycosyltransferases (PGTs) that differ in sugar donor specificity to catalyze either glucose or xylose transfer to tetrahydrobiopterin were studied here to uncover the structural determinants necessary for the specificity. The importance of the C-terminal domain and its residues 218 and 258 that are different between the two PGTs was assessed via structure-guided domain swapping or single and dual amino acid substitutions. Catalytic activity and selectivity were altered in all the mutants (2 chimeric and 6 substitution) to accept both UDP-glucose and UDP-xylose. In addition, the wild type activities were improved 1.6-4.2 fold in 4 substitution mutants and activity was observed towards another substrate UDP-N-acetylglucosamine in all the substitution mutants from CY-007 PGT. The results strongly support essential role of the C-terminal domain and the two residues for catalysis as well as sugar donor specificity, bringing insight into the structural features of the PGTs.

• BMB Reports
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• v.40 no.4
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• pp.539-546
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• 2007

The lysozymes encoded by bacteriophage T7 and K11 are both bifunctional enzymes sharing an extensive sequence homology (75%). The constructions of chimeric lysozymes were carried out by swapping the N-terminal and C-terminal domains between phage T7 and K11 lysozymes. This technique generated two chimeras, T7K11-lysozyme (N-terminal T7 domain and C-terminal K11 domain) and K11T7-lysozyme (N-terminal K11 domain and C-terminal T7 domain), which are both enzymatically active. The amidase activity of T7K11-lysozyme is comparable with the parental enzymes while K11T7-lysozyme exhibits an activity that is approximately 45% greater than the wild-type lysozymes. Moreover, these chimeric constructs have optimum pH of 7.2-7.4 similar to the parental lysozymes but exhibit greater thermal stabilities. On the other hand, the chimeras inhibit transcription comparable with the parental lysozymes depending on the source of their N-terminals. Taken together, our results indicated that domain swapping technique localizes the N-terminal region as the domain responsible for the transcription inhibition specificity of the wild type T7 and K11 lysozymes. Furthermore, we were able to develop a simple and rapid purification scheme in purifying both the wild-type and chimeric lysozymes.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.559-559
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• 2005

• Molecules and Cells
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• v.19 no.2
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• pp.246-255
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• 2005

Retroviral integrases insert viral DNA into target DNA. In this process they recognize their own DNA specifically via functional domains. In order to analyze these functional domains, we constructed six chimeric integrases by swapping domains between HIV-1 and HFV integrases, and two point mutants of HFV integrase. Chimeric integrases with the central domain of HIV-1 integrase had strand transfer and disintegration activities, in agreement with the idea that the central domain determines viral DNA specificity and has catalytic activity. On the other hand, chimeric integrases with the central domain of HFV integrase did not have any enzymatic activity apart from FFH that had weak disintegration activity, suggesting that the central domain of HFV integrase was defective catalytically or structurally. However, these inactive chimeras were efficiently complemented by the point mutants (D164A and E200A) of HFV integrase, indicating that the central domain of HFV integrase possesses potential enzymatic activity but is not able to recognize viral or target DNA without the help of its homologous N-terminal and C-terminal domains.

• The Journal of Information Technology
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• v.5 no.4
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• pp.155-164
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• 2002

MPLS is a integrated technology by using routing function and label swapping in the network layer. Based on the previous forwarding equivalence classes, it adds the fixed length label in ingress of the MPLS domain. For the routing, without the packet header information, it uses label for the forwarding decisions. In this paper, traffic engineering requirements in the MPLS internet will be setup. The traffic engineering function have to be performed previously with the network topology. In addition to, we presents the IP network topology and main function with MPLS signaling protocol.

• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.788-792
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• 2012

We report the computational structural simulation of the Cry1Ab19 toxin molecule from B. thuringiensis BtX-2 based on the structure of Cry1Aa1 deduced by x-ray diffraction. Validation results showed that 93.5% of modeled residues are folded in a favorable orientation with a total energy Z-score of -8.32, and the constructed model has an RMSD of only $1.13{\AA}$. The major differences in the presented model are longer loop lengths and shortened sheet components. The overall result supports the hierarchical three-domain structural hypothesis of Cry toxins and will help in better understanding the structural variation within the Cry toxin family along with facilitating the design of domain-swapping experiments aimed at improving the toxicity of native toxins.

• Microbiology and Biotechnology Letters
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• v.48 no.1
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• pp.24-31
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• 2020

Five genes (mxbDM, mxcQE and mxaB) are responsible for the transcription of methanol oxidation genes in Methylobacterium strains. Among these, MxbDM and MxcQE constitute the two-component system (TCS) regulating methanol metabolism. In this study, we integrated the methanol-sensing domain of MxbD and MxcQ with the EnvZ/OmpR from Escherichia coli. The domain-swapping strategy resulted in chimeric histidine kinases (HK's) MxbDZ and MxcQZ AM1 containing recombinant E. coli. Real-time quantitative PCR was used to monitor OmpC expression mediated by the chimeric HK and response regulator (RR) OmpR. Further, an ompC promoter based fluorescent biosensor for sensing methanol was developed. GFP fluorescence was studied both qualitatively and quantitatively in response to environmental methanol. GFP measurement also confirmed ompC expression. Maximum fluorescence was observed at 0.05% methanol and 0.01% methanol using MxbDZ and MxcQZ AM1, respectively. Thus the chimeric HK containing E. coli were found to be highly sensitive to methanol, resulting in a rapid response making them an ideal sensor.

• ETRI Journal
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• v.37 no.3
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• pp.595-605
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• 2015

This paper proposes a robust, imperceptible block-based digital video watermarking algorithm that makes use of the Speeded Up Robust Feature (SURF) technique. The SURF technique is used to extract the most important features of a video. A discrete multiwavelet transform (DMWT) domain in conjunction with a discrete cosine transform is used for embedding a watermark into feature blocks. The watermark used is a binary image. The proposed algorithm is further improved for robustness by an error-correction code to protect the watermark against bit errors. The same watermark is embedded temporally for every set of frames of an input video to improve the decoded watermark correlation. Extensive experimental results demonstrate that the proposed DMWT domain video watermarking using SURF features is robust against common image processing attacks, motion JPEG2000 compression, frame averaging, and frame swapping attacks. The quality of a watermarked video under the proposed algorithm is high, demonstrating the imperceptibility of an embedded watermark.

• Korean Journal of Remote Sensing
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• v.17 no.4
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• pp.297-305
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• 2001

Ocean satellite altimetry-implied free-air gravity anomalies have had the shortest wavelengths removed during the processing to generate the optimal solution between multiple radar altimeter missions. ERS-1 168day mission altimetry was residualized to a reference geoid surface generated by integrating Anderson & Knudsen’s free-air gravity anomalies for the Barents Sea. The altimetry tracks were reduced and filtered to extract the shortest wavelengths (between 4 and 111 km) from both ascending and descending tracks, respectively. These data were recombined using existing quadrant-swapping techniques in the wavenumber domain to generate a correlated, high frequency gravity field related to the local geologic sources. This added-value surface adjusted the reference free-air gravity anomalies to better reflect features in the gravity field at a wavelength related to the distance between altimetry ground tracks.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.4
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• pp.337-349
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• 2013

In this paper, we propose a new mobility management scheme, called i-FP(intelligent Fast PMIPv6). Our proposed i-FP scheme is addressed for solving the existing local mobility management problems from legacy frameworks. To move MN(Mobile Node) to other networks in one domain, i-FP employs three network entities which are extended from PMIPv6(Proxy Mobile IPv6), LMA(Local Mobility Anchor), MAG(Mobile Access Gateway) and MN. In i-FP, the three network entities can reduce the handover delay time of MNs. Also, i-FP uses an IP header swapping mechanism to avoid the traffic overhead and improve the throughput of network. To evaluate the performance of i-FP, we analyze our i-FP, HMIPv6(Hierarchical Mobile IPv6) and PMIPv6 which are legacy protocols of local mobility management in terms of various parameters. Finally, our i-FP scheme shows good performance(reduction of routing hops 10.2%, signaling costs 58.5% and handover delay 16.3%) than other network schemes for the total cost.