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Effects of chronic caloric restriction on kidney and heart redox status and antioxidant enzyme activities in Wistar rats

  • Dutra, Marcio Ferreira;Bristot, Ivi Juliana;Batassini, Cristiane;Cunha, Nubia Broetto;Vizuete, Adriana Fernanda Kuckartz;Souza, Daniela Fraga De;Moreira, Jose Claudio Fonseca;Goncalves, Carlos-Alberto
    • BMB Reports
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    • v.45 no.11
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    • pp.671-676
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    • 2012
  • Caloric restriction (CR) has been associated with health benefits and these effects have been attributed, in part, to modulation of oxidative status by CR; however, data are still controversial. Here, we investigate the effects of seventeen weeks of chronic CR on parameters of oxidative damage/modification of proteins and on antioxidant enzyme activities in cardiac and kidney tissues. Our results demonstrate that CR induced an increase in protein carbonylation in the heart without changing the content of sulfhydryl groups or the activities of superoxide dismutase and catalase (CAT). Moreover, CR caused an increase in CAT activity in kidney, without changing other parameters. Protein carbonylation has been associated with oxidative damage and functional impairment; however, we cannot exclude the possibility that, under our conditions, this alteration indicates a different functional meaning in the heart tissue. In addition, we reinforce the idea that CR can increase CAT activity in the kidney. Moreover, CR caused an increase in CAT activity in kidney, without changing other parameters. Protein carbonylation has been associated with oxidative damage and functional impairment; however, we cannot exclude the possibility that, under our conditions, this alteration indicates a different functional meaning in the heart tissue. In addition, we reinforce the idea that CR can increase CAT activity in the kidney.

Effects of Stocking Density and Dissolved Oxygen Concentration on the Growth and Hematology of the Parrotfish Oplegnathus fasciatus in a Recirculating Aquaculture System (RAS) (순환여과시스템에서 사육밀도와 용존산소 농도가 돌돔(Oplegnathus fasciatus)의 성장과 혈액성상에 미치는 영향)

  • Kim, Pyong-Kih
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.747-752
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    • 2011
  • The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

A Dynamic Update Engine of IPS for a DoS Attack Prevention of VoIP (VoIP의 DoS공격 차단을 위한 IPS의 동적 업데이트엔진)

  • Cheon, Jae-Hong;Park, Dea-Woo
    • KSCI Review
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    • v.14 no.2
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    • pp.235-244
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    • 2006
  • This paper attacked the unknown DoS which mixed a DoS attack, Worm and the Trojan horse which used IP Source Address Spoofing and Smurf through the SYN Flooding way that UDP, ICMP, Echo, TCP Syn packet operated. the applications that used TCP/UDP in VoIP service networks. Define necessity of a Dynamic Update Engine for a prevention, and measure Miss traffic at RT statistics of inbound and outbound parts in case of designs of an engine at IPS regarding an Self-learning module and a statistical attack spread. and design a logic engine module. Three engines judge attack grades (Attack Suspicious, Normal), and keep the most suitable filtering engine state through AND or OR algorithms at Footprint Lookup modules. A Real-Time Dynamic Engine and Filter updated protected VoIP service from DoS attacks, and strengthened Ubiquitous Security anger, and were turned out to be.

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A Dynamic Update Engine of IPS for a DoS Attack Prevention of VoIP (VoIP의 DoS공격 차단을 위한 IPS의 동적 업데이트엔진)

  • Cheon, Jae-Hong;Park, Dea-Woo
    • Journal of the Korea Society of Computer and Information
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    • v.11 no.6 s.44
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    • pp.165-174
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    • 2006
  • This paper attacked the unknown DoS which mixed a DoS attack, Worm and the Trojan horse which used IP Source Address Spoofing and Smurf through the SYN Flooding way that UDP, ICMP, Echo, TCP Syn packet operated, the applications that used TCP/UDP in VoIP service networks. Define necessity of a Dynamic Update Engine for a prevention, and measure Miss traffic at RT statistics of inbound and outbound parts in case of designs of an engine at IPS regarding an Self-learning module and a statistical attack spread, and design a logic engine module. Three engines judge attack grades (Attack, Suspicious, Normal), and keep the most suitable filtering engine state through AND or OR algorithms at Footprint Lookup modules. A Real-Time Dynamic Engine and Filter updated protected VoIP service from DoS attacks, and strengthened Ubiquitous Security anger, and were turned out to be.

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Functional Safety and Model-Based Systems Engineering - focusing on ISO 26262/DO-178C (기능 안전과 모델기반 시스템엔지니어링 - ISO 26262/DO-178C 중심으로)

  • Park, Joongyong;Paek, Seung-Kil
    • Journal of the Korean Society of Systems Engineering
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    • v.13 no.1
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    • pp.57-65
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    • 2017
  • Recently, the ratio of electrical part and embedded software has grown in automotive industry. ISO 26262, 'Road Vehicles - Functional Safety', was published to guide development of automotive electrical and electronic part in 2011. This paper describes definition of functional safety and analyzes ISO 26262. The comparison of ISO 26262 and DO-178C is made, then difference between them is identified. DO-178C provides guidance for the production of software for airborne system and equipment. The core of DO-178C is a relatively minor update to the previous DO-178B, however, the big changes are captured in the supplemented documents such as DO-331, 'Model-Based Development and Verification Supplement to DO-178C and DO-278A'. Model-based design is important to develop automotive and aircraft meeting the guidelines of ISO 26262 and DO-178C. In this paper, the sample case of applying MBSE(Model-Based Systems Engineering) to AVCS(Active Vibration Control System) software development is discussed.

Limiting user process method based on PAM against DoS attacks (DoS 공격에 대비한 PAM 기반 사용자 프로세스 제한 기법)

  • Lee, Jae-Ung;Jung, Sung-Jae;Bae, Yu-Mi;Jang, Rae-Young;Soh, Woo-Young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.05a
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    • pp.309-312
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    • 2016
  • Considering that interkorean relations got worse and worse recently, cyber terror of North Korea has seriously become a possibility. Therefore, DoS(Denial of Service), a typical way of cyber terror, is becoming a big issue. Consequently, people are growing more and more interested in information security. Internal DoS attacks, out of a variety of ways of Dos attacks, include disks and memories and shortages of process resources. PAM(Pluggable Authentication Module) is one of the ways of preventing internal DoS attacks in Linux system. This paper provides with a method to internally respond to dos attacks and efficiently prevent shortages of resources by utilizing PAM.

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Effects of Dissolved Oxygen Level on Avermectin $B_{1a}$ Production by Streptomyces avermitilis in Computer-Controlled Bioreactor Cultures

  • Song, Sung-Ki;Jeong, Yong-Seob;Kim, Pyeung-Hyeun;Chun, Gie-Taek
    • Journal of Microbiology and Biotechnology
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    • v.16 no.11
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    • pp.1690-1698
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    • 2006
  • In order to investigate the effect of dissolved oxygen (DO) level on AVM $B_{1a}$ production by a high yielding mutant of Streptomyces avermitilis, five sets of bioreactor cultures were performed under variously controlled DO levels. Using an online computer control system, the agitation speed and aeration rate were automatically controlled in an adaptive manner, responding timely to the oxygen requirement of the producer microorganism. In the two cultures of DO limitation, the onset of AVM $B_{1a}$ biosynthesis was observed to casually coincide with the fermentation time when oxygen-limited conditions were overcome by the producing microorganism. In contrast, this phenomenon did not occur in the parallel fermentations with DO levels controlled at around 30% and 40% throughout the entire fermentation period, showing an almost growth-associated mode of AVM $B_{1a}$ production: AVM $B_{1a}$ biosynthesis under the environments of high DO levels started much earlier than the corresponding oxygen-limited cultures, leading to a significant enhancement of AVM $B_{1a}$ production during the exponential stage. Consequently, approximately 6-fold and 9-fold increases in the final AVM $B_{1a}$ production were obtained in 30% and 40% DO-controlled fermentations, respectively, especially when compared with the culture of severe DO limitation (the culture with 0% DO level during the exponential phase). The production yield ($Y_{p/x}$), volumetric production rate (Qp), and specific production rate (${\bar{q}}_p$) of the 40% DO-controlled culture were observed to be 14%, 15%, and 15% higher, respectively, than those of the parallel cultures that were performed under an excessive agitation speed (350 rpm) and aeration rate (1 vvm) to maintain sufficiently high DO levels throughout the entire fermentation period. These results suggest that high shear damage of the high-yielding strain due to an excessive agitation speed is the primary reason for the reduction of the AVM $B_{1a}$ biosynthetic capability of the producer. As for the cell growth, exponential growth patterns during the initial 3 days were observed in the fermentations of sufficient DO levels, whereas almost linear patterns of cell growth were observed in the other two cultures of DO limitation during the identical period, resulting in apparently lower amounts of DCW. These results led us to conclude that maintenance of optimum DO levels, but not too high to cause potential shear damage on the producer, was crucial not only for the cell growth, but also for the enhanced production of AVM $B_{1a}$ by the filamentous mycelial cells of Streptomyces avermitilis.

A Study of N-IDS Detection regarding a DoS Attack and Packet Analysis (DoS공격에 대한 N-IDS 탐지 및 패킷 분석 연구)

  • Chun, Woo-Sung;Park, Dae-Woo
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2008.06a
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    • pp.175-182
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    • 2008
  • 2008년에 있었던 우리나라 금융기관과 정부기관에 대한 DoS 공격에 대한 연구이다. 실험실 환경에서 실제 DoS 공격 툴을 이용하여 공격을 실시한다. DoS 공격을 탐지하기 위하여 네트워크 상에서 Snort를 이용한 N-IDS를 설치하고, 패킷을 탐지하기 위한 Winpcap과 패킷의 저장 및 분석하기 위한 MySQL, HSC, .NET Framework 등을 설치한다. e-Watch 등의 패킷 분석 도구를 통해 해커의 DoS 공격에 대한 패킷량과 TCP, UDP 등의 정보, Port, MAC과 IP 정보 등을 분석한다. 본 논문 연구를 통하여 유비쿼터스 정보화 사회의 역기능인 사이버 DoS, DDoS 공격에 대한 자료를 분석하여 공격자에 대한 포렌식자료 및 역추적 분석 자료를 생성하여 안전한 인터넷 정보 시스템을 확보하는데 의의가 있다.

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Evaluation of DoP-CPD Classification Technique and Multi Looking Effects for RADARSAT-2 Images

  • Lee, Kyung-Yup;Oh, Yi-Sok;Kim, Youn-Soo
    • Korean Journal of Remote Sensing
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    • v.28 no.3
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    • pp.329-336
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    • 2012
  • This paper give further assessment on the original DoP-CPD classification scheme. This paper provides some additional comparative study on the DoP-CPD with H/A/alpha classifier in terms of multi look effects and classification performances. The statistics and multi looking effects of the DoP and CPD were analyzed with measured polarimetric SAR data. DoP-CPD is less sensitive to the number of averaging pixels than the entropy-alpha technique. A DoP-CPD diagram with appropriate boundaries between six different classes was then developed based on the data analysis. A polarimetric SAR image DoP-CPD classification technique is verified with C-band polarimetric RADARSAT-2 images.