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Anatomical Studies on the Features of Rays in Compression Wood of Korean Red Pine(Pinus densiflora S. et Z.) (소나무(Pinus densiflora S. et Z.) 압축이상재(壓縮異常材)의 방사조직(放射組織) 특성(特性)에 관한 해부학적(解剖學的) 연구(硏究))

  • Chung, Youn Jib;Lee, Phil Woo
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.119-131
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    • 1989
  • This experiment was executed to investigate and compare qualitative and quantitative anatomical features in compression wood, opposite wood, and side wood formed in a bent stem, a straight branch, and an exposed horizontal root of Korean red pine(Pinus densiflora S. et Z.). The respective four discs containing compression wood taken at 20cm interval both in stem and branch as well as a disc containing well developed compression wood from horizontal root were analyzed. Percentage of compression wood and eccentricity showed decreasing tendency with the increasing distance in height direction of stem and length direction of branch. The qualitative anatomical features of compression wood appeared to differ from those of side and opposite wood in very gradual tracheid transition from earlywood to latewood, roundish tracheid shape on cross surface, tracheid distortion at tip on radial surface, existence of intercellular space, and helical cavity in tracheid wall. And the differences in these qualitative features among the compression wood, opposite wood, and side wood became less intensive with the decreasing trends in percentage of compression wood and eccentricity. The quantitative anatomical features in compression wood also appeared to be wider in that respective widths of fusiform and uniseriate ray than those of opposite and side wood, but the heights of fusiform and uniseriate ray in compression wood were smaller than in opposite and side wood. The number of horizontal resin canal(fusiform ray) and uniseriate ray, however, showed no differences among the compression wood, opposite wood, and side wood. And the number of vertical resin canal in unit area, $4{\pi}mm^2$ of compression wood was fewer than that in opposite wood, whereas numerous vertical resin canals contained in a growth ring. These rays of compression wood seemed to be characterized by smaller height and wider width than those of opposite and side wood.

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Study of Factors Controlling Exposure Dose and Image Quality of C-arm in Operation Room according to Detector Size of It (Mainly L-Spine AP Study) (수술 중 C-Arm Neutral AP 검사 시 조절인자에 따른 피폭선량 및 화질비교(L-Spine AP검사를 기준으로))

  • CHOI, Sung-Hyun;JO, Hwang-Woo;Dong, Kyung-Rae;Chung, Woon-Kwan;Choi, Eun-Jin;Song, Ha-jin
    • Journal of Radiation Industry
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    • v.9 no.2
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    • pp.85-90
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    • 2015
  • Purpose: Time of operation has been reduced and accuracy of operation has been improved since C-arm, which offer real-time image of patient, was introduced in operation room. However, because of the contamination of patient, C-arm could not be used more appropriately. Therefore, this study is to know factors of controlling exposure dose, image quality and the exposed dose of health professional in operation room. Materials and methods: Height of Wilson frame (bed for operation) was fixed at 130 cm. Then, Model 76-2 Phantom, which was set by assembling manual of Fluke Company, was set on the bed. Head/Spine Fluoroscopy AEC mode was set for exposure condition. According to detector size of C-arm, the absorbed dose per min was measured in the 7 steps OFD (cm) from 10 cm to 40 cm (10, 15, 20, 25, 30, 35, 40 cm). In each step of OFD, the absorbed dose per min of same diameter of collimation was measured. Moreover, using Nero MAX Model 8000, exposure dose per min was measured according to 3 step of distance from detector (20 cm, 60 cm, 100 cm). Finally, resolution was measured by CDRH Disc Phantom and magnification of each OFD was measured by aluminum stick bar. Result: According to detector size of C-arm, difference of absorbed dose shows that the dose of 20 cm OFD is 1.750 times higher than the dose of 40 cm OFD. It means that the C-arm, which has smaller size of detector, shows the bigger difference of absorbed dose per min (p<0.05). In the difference of absorbed dose in the same step of OFD (from 20 cm to 40 cm), the absorbed dose of 9 inch detect or C-arm was 1.370 times higher than 12 inch' s (p<0.05). When OFD was set to 20 cm OFD, the absorbed dose of non-collimation case was approximately 0.816 times lower than the absorbed dose of collimation cases (p<0.05). When the distance was 20 cm from detector, exposed does includes first-ray and scatter-ray. When the distance was 60 cm and 100 cm from detector, exposed does includes just scatter-ray. So, there was the 2.200 times difference of absorbed does. Finally, when OFD was increased, spatial resolution was 4 to 5 step was increased. However, low contrast resolution was not relative. Moreover, there was 1.363 times difference of magnification (p<0.05). Conclusion: When C-Arm is used, avoiding contamination of patient is more important factor than reducing exposed dose of health professional in operation room. Just controlling exposure time is just way to reduce the exposed does of workers. However, in the case, non-probability influence could be occurred. Therefore, this study proved that the exposed dose will be reduced if the factors such as using small detector size of C-arm, setting OFD from 20 cm to 25 cm and non-collimating. Moreover, dose management of C-arm in the non-interesting area will be considered additionally.

Effect of antioxidation and antibacterial activity on crude extract and Characterization of American Cockroaches (Periplaneta americana L.) in Korea (국내 서식 미국바퀴(Periplaneta americana L.)의 특성 및 추출물의 항산화·항균 효과)

  • Kim, Jung-Eun;Kim, Seon-Gon;Kang, Sung-Ju;Kim, Chun-Sung;Choi, Yong-Soo
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.135-142
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    • 2015
  • The American cockroaches, Periplaneta americana L. was the most important worldwide pest species. It has been an public health problems. We were determinated life cycle and extraction of crude extracts by chemical reagents from cockraches (P. americana L.). The extracted crude solution has been antibacterial activity to gram negative bacteria (Pseudomonas aeruginosa, $6.44{\pm}1.03mm$), gram positive bacteria (Bacillus subtilis, $1.88{\pm}0.40mm$), and fungus (Candida albicans, $5.61{\pm}0.57mm$) using radial diffusion assay. We were analysed of up-regulation of Glutathione-S-transferases (GSTs) stimulation, indicating that antioxidantial protein from various classes are simultaneously expressed in a single insect upon infection or injury. The gene from Periplaneta americana L. were cloned, analysed sequence, and measured protein expression by Real Time PCR (Polymerase Chain Reaction).

Antibacterial and Antioxidant Potential of Methanol Extract of Viburnum sargentii Seeds (Viburnum sargentii 종자 메탄올 추출물의 항균 및 항산화 활성에 대한 연구)

  • Patil, Maheshkumar Prakash;Seong, Yeong-Ae;Kang, Min-jae;Singh, Alka Ashok;Niyonizigiye, Irvine;Kim, Gun-Do;Lee, Jong-Kyu
    • Journal of Life Science
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    • v.29 no.6
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    • pp.671-678
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    • 2019
  • Antibacterial and antioxidant activities of plant sources have attracted a wide range of interest across the world over the last decade. This is due to the growing concern for safe and alternative sources of antibacterial and antioxidant agents. In this study, we focused on the antibacterial and antioxidant activities and the chemical composition of a methanol extract from Viburnum sargentii seeds. The chemical composition was determined by gas chromatography-mass spectroscopy (GC-MS), and the antibacterial activity was screened by a disc diffusion assay. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the microbroth dilution and spread plate method, respectively. The V. sargentii extract showed growth inhibition activity on all tested Gram-positive (Listeria monocytogenes, Staphylococcus aureus, and Staphylococcus saprophyticus) and Gram-negative (Escherichia coli, Pseudomonas putida, and Proteus vulgaris) pathogenic bacteria. The MIC and MBC ranged from 0.156~1.25 mg/ml for Gram-positive and 0.625~5.0 mg/ml for Gram-negative tested bacteria. The GC-MS results revealed the presence of several phytochemicals such as ${\beta}-sitosterol$ and vitamin E, which are known for their pharmacological applications. The antioxidant activities of V. sargentii extract were investigated by three different methods: the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, the reducing power assay, and the total antioxidant capacity assay. The results showed a concentration-dependent antioxidant potential for all three used methods. In sum, our findings suggest that the methanol extract of V. sargentii seeds has the potential to inhibit the growth of pathogenic bacteria and provide antioxidant compounds, making it therefore worthy of further investigation.

Studies on Biological Activity of Woad Extractives (XV) - Antimicrobial and antioxidative activities of extracts from diverse families - (수목 추출물의 생리활성에 관한 연구(XV) - 과별(科別)에 따른 항균 및 항산화 활성 -)

  • Lee, Sung-Suk;Lee, Hak-Ju;Choi, Don-Ha
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.4
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    • pp.8-17
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    • 2004
  • Antifungal, antibacterial, and antioxidative activities of ethanol extracts from 65 families 263 species were investigated to select tree species for the utilization of natural fungicide or preservative resources. The antifungal activities of extracts from wood, leaf and bark were measured as hyphal growth inhibition rate using four plant pathogenic and five wood rotting fungi. High inhibitory effect on the fungi growth was found in five species of Pinaceae (Pinus koraiensis, P. rigida, P. densiflora, P. banksiana. Cedrus deodara), three species of Cupressaceae (Juniperus rigida, J. chinensis, Chamaecyparis obtusa) and three species of Leguminosae (Albizzia julibrisssin, Sophora japonica, Maackia amurensis), respectively. Antibacterial activities of ethanol extracts were determined by means of disc-agar plate diffusion method using three gram-positive and five gram-negative bacteria. The ethanol extracts, which showed prominent effect on the suppression of bacteria growth, were six species of Betulaceae (Carpinus tschonoskii, C. coreana, C. laxiflora, Alnus hirsuta, A. firma, Betula schmidtii), five species of Fagaceae (Castanopsis cuspidata var. sieboldii, Quercus serrata, Q. mongolica, Q aliena, C crenata), four species of Euphorbiaceae (Aleurites fordii, Sapium sebiferum, S japonicum Mallotus japonicus) and three species of Elaeagnaceae (Elaeagnus umbellata, Elaeagnus glanbra, Elaeagnus macrophylla). According to these results, the extracts from Zelkova serrata, Pinus densiflora, Maackia amurensis, Chamaecyparis obtusa and Juniperus chinensis could be available for natural fungicide or food preservatives, because ethanol extracts from these species indicated excellent antifungal and antibacterial activities. In order to test antioxidative activities of ethanol extracts, free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH). Free radical scavenging activity was proved very high in the extracts of eight species of Rosaceae (Eriobotrya japonica, Prunus takesimensis, P yedoensis, P padus, P armeniaca var. ansu, Chaenomeles sinensis, Stephanandra incisa, Rosa multiflora) and five species of Ericaceae (Rhododenron mucronulatum, R. scblippenbacbii, R. yedoense var. poukhanense, Vaccinium bracteatum, V oldbami), resvectively. It turned out from this study that only six species among 48 species of Rosaceae showed less than 80% free radical scavenging activity. As a consequences, it could be deduced that the components effective on antioxidative activity commonly exist in Rosaceae plant family.

Is It Appropriate to Insert Pedicle Screws at an Infected Vertebral Body in the Treatment of Lumbar Pyogenic Spondylodiscitis? (요추부 화농성 척추염의 수술적 치료: 이환된 추체에 척추경 나사 고정이 타당한가?)

  • Na, Hwa-Yeop;Jung, Yu-Hun;Lee, Joo-Young;Kim, Hyung-Do
    • Journal of the Korean Orthopaedic Association
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    • v.56 no.5
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    • pp.419-426
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    • 2021
  • Purpose: In the surgical treatment of pyogenic lumbar spondylodiscitis, screw insertion at the affected vertebra has been avoided because of biofilm formation, and the risk of infection recurrence. The authors analyzed the success rate of infection treatment while minimizing the number of instrumented segments by inserting pedicle screws into the affected vertebrae. Therefore, this study examined the usefulness of this technique. Materials and Methods: From January 2000 to June 2018, among patients with pyogenic lumbar spondylodiscitis treated surgically, group A consisted of patients with pedicle screws inserted directly at the affected vertebrae (28 cases), and group B underwent fusion by inserting screws at the adjacent normal vertebrae due to bone destruction of the affected vertebral pedicle (20 cases). The classified clinical results were analyzed retrospectively. All patients were treated via the posterior-only approach, so the affected disc and sequestrum were removed. Posterior interbody fusion was performed with an autogenous strut bone graft, and the segments were then stabilized with pedicle screw systems. The hospitalization period, operation time, amount of blood loss, EQ-5D index, duration of intravenous antibiotics, and the clinical and radiological results were analyzed. Results: In group A, the number of instrumented segments, operation time, blood loss, and EQ-5D index at one month postoperatively showed significant improvement compared to group B. There were no significant differences in the duration of antibiotic use, hospitalization, radiological bone union time, sagittal angle correction rate, and recurrence rate. Conclusion: Minimal segmental fixation, in which pedicle screws were inserted directly into the affected vertebrae through the posterior approach, reduced the surgery time and blood loss, preserved the lumbar motion by minimizing fixed segments and showed rapid recovery without spreading or recurrence of infection. Therefore, this procedure recommended for the surgical treatment of lumbar pyogenic spondyodiscitis.

Comparison of Color Stability and Surface Roughness of 3D Printing Resin by Polishing Methods (연마 방법에 따른 3D 프린팅 레진의 색조 안정성과 표면 조도의 비교)

  • Heeju Kim;Yujin Kim;Jongsoo Kim;Joonhaeng Lee;Mi Ran Han;Jisun Shin;Jongbin Kim
    • Journal of the korean academy of Pediatric Dentistry
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    • v.50 no.2
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    • pp.205-216
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    • 2023
  • This study aimed to compare the color stability and surface roughness of three-dimensional (3D) printing resin according to polishing methods. 3D-printed resin specimens were fabricated at TC-80DP (Graphy, Seoul, Korea) with a stereolithography 3D printer, and the specimens were divided into three groups, each of which was not polished, was polished using Enhance®, and was polished using a Sof-LexTM disc. The CIE L*a*b* value and the surface roughness of each group were measured and immersed in artificial saliva and orange juice after 0, 1, 7, 30, and 60 days, and the color difference (ΔE*) was calculated. As a result of the study, no noticeable color change was observed in artificial saliva, but a noticeable color change was demonstrated in orange juice after 60 days of immersion, and the difference was significant. In the Sof-LexTM group, surface roughness according to the solution was found to be significantly higher in the orange juice than that in artificial saliva. No significant difference in color change was found according to the polishing method, but surface roughness was significantly lower in the Sof-LexTM group than both that of the unpolished group and that of the Enhance® group. Nevertheless, all groups exhibited clinically acceptable properties regardless of their higher surface roughness than the threshold for plaque accumulation. Overall, this study recommends utilizing Sof-LexTM for polishing 3D printing resin when used in primary anterior tooth coverage.

Evaluation of Antimicrobial Activities of Rhubarb Extracts on Putrefactive Microorganisms Related to Soybean Curd (Doobu) (두부 부패 미생물에 대한 대황(Rhubarb) 추출물의 항균 활성 평가)

  • Kim, Chul-Jai;Suh, Hee-Ji;Chung, Hee-Sook
    • Journal of the Korean Society of Food Culture
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    • v.21 no.2
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    • pp.225-231
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    • 2006
  • 대황(Rhubarb)은 중국과 일본에서 혈액 순환제, 진통제, 신장치료제 등으로 전통적으로 사용되고 있는 약용식물이다. 이 연구에서는 한국산 R. undulatum L.의 뿌리 추출물, 미국산 R. rhabarbarum L.과 중국산 R. palmatum L.의 줄기 추출물의 두부 부패에 관여하는 미생물에 대한 항균 활성을 평가하였다. Rhubarb의 최적 추출을 위해서 각각 50%, 70%, 80% 에탄올을 용매로 사용하였으며, 항균 활성은 Kirby-Bauert test, minimum inhibitory concentration (MIC)과 minimum bactericide concentration (MBC)에 의해서 평가되었다. Kirby-Bauert test 결과, R. undulatum L.의 뿌리 추출물은 대부분 두부 부패균에 대해서 항균 활성을 가지는 것으로 나타났으며, R. rhabarbarum L.의 줄기 추출물의 경우는 $20{\mu}g/disc$의 농도에서는 항균 활성이 거의 나타나질 않았으나, 고농도로 갈수록 높은 항균 활성을 나타내었다. 또한 R. palmatum L.의 줄기 추출물은 고농도로 갈수록 항균 활성이 높아졌으나, Pseudomonas aeruginosa에 관해서는 항균 활성이 나타나지 않았다. MIC와 MBC에 의한 항균 활성 평가실험에서는, 세 종류의 rhubarb 추출물 중에서 phenolic 화합물이 가장 많이 함유되어 있는 R. undulatum L.의 뿌리 추출물보다 R. rhabarbarum L.의 줄기 추출물이 MIC와 MBC 값이 낮았다. 이는 phenolic 화합물의 양 이외에 낮은 pH가 항균 활성에 영향을 준 것으로 판단된다. 본 연구를 통해서 rhubarb 추출물은 두부 부패에 관여하는 미생물에 대해서 항균 활성을 가지는 것으로 나타났으며, 천연 항균제로써 식품에 적용 가능성이 있는 것으로 평가되었다. 87.1%이었으며, 아침을 매일 먹는 채식남학생은 78.8%, 비채식남학생은 33.3%, 채식여학생 47.1%, 비채식여학생 39.6%이었다. 또한 식사의 양은 과식한다는 응답이 채식남학생 24.2%, 비채식남학생 38.1%, 채식여학생은 29.4%, 비채식여학생 40.6%으로 비책식군의 과식율이 높았다. 5. 식품 섭취빈도는 두부 및 콩제품을 매일 섭취하는 경우는 채식남학생 54.6%, 비채식남학생 16.7%, 채식여학생은 38.2%, 비채식여학생이 16.8%이었다. 우유 및 유제품을 매일 섭취하는 경우는 채식남학생 6.1%, 비채식남학생 33.3%, 채식여학생 14.7%, 비채식여학생은 21.8%이었으며, 녹차, 커피 등 차를 마시지 않는다는 비율은 채식남학생 69.7%, 비채식남학생 28.6%, 채식여학생 29.4%, 비채식여학생 25.7%이었다. 인스턴트 식품을 매일 섭취한다는 응답율이 채식남학생 9.1%, 비채식남학생 21.4%, 채식여학생은 17.7%, 비채식여학생은 14.9%이었다. 6. 운동, 체중 조절 등에 대한 조사 결과 항상 운동을 하는 경우는 채식남학생 30.3%, 비채식남학생 28.6%, 채식여학생 14.7%, 비채식여학생 18.8%이었으며 운동시간은 $1{\sim}2$시간 하는 경우는 채식남학생 30.3%, 비채식남학생 38.1%, 채식여학생은 8.8%, 비채식여학생은 17.8%이었다. 체중에 만족하는 정도를 보면 채식남학생 57.6%, 비채식남학생 23.8%, 채식여학생은 23.5%, 비채식여학생은 15.8%가 만족한다고 하였다. 체중 조절 경험에서 경험이 있는 경우가 채식남학생 3.0%, 비채식남학생 31.0%, 채식여학생은 23.5%, 비채식여학생 31.7%이었다. 7. 골밀도 BQI값과와 몇가지 요인의 상관관계를 살펴보았을때, 채식남학생은 영양보충제의 섭취와 유의적인 양의 상관관계를, 해조류의 섭취정도와 유의적인 음의 상관관계를 나타내었다.

Is a Camera-Type Portable X-Ray Device Clinically Feasible in Chest Imaging?: Image Quality Comparison with Chest Radiographs Taken with Traditional Mobile Digital X-Ray Devices (카메라형 휴대형 X선 장치는 흉부 촬영에서 임상적 사용이 가능한가?: 기존의 이동형 디지털 X선 장치로 촬영한 흉부 X선 사진과 영상품질 비교)

  • Sang-Ji Kim;Hwan Seok Yong;Eun-Young Kang;Zepa Yang;Jung-Youn Kim;Young-Hoon Yoon
    • Journal of the Korean Society of Radiology
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    • v.85 no.1
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    • pp.138-146
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    • 2024
  • Purpose To evaluate whether the image quality of chest radiographs obtained using a camera-type portable X-ray device is appropriate for clinical practice by comparing them with traditional mobile digital X-ray devices. Materials and Methods Eighty-six patients who visited our emergency department and underwent endotracheal intubation, central venous catheterization, or nasogastric tube insertion were included in the study. Two radiologists scored images captured with traditional mobile devices before insertion and those captured with camera-type devices after insertion. Identification of the inserted instruments was evaluated on a 5-point scale, and the overall image quality was evaluated on a total of 20 points scale. Results The identification score of the instruments was 4.67 ± 0.71. The overall image quality score was 19.70 ± 0.72 and 15.02 ± 3.31 (p < 0.001) for the mobile and camera-type devices, respectively. The scores of the camera-type device were significantly lower than those of the mobile device in terms of the detailed items of respiratory motion artifacts, trachea and bronchus, pulmonary vessels, posterior cardiac blood vessels, thoracic intervertebral disc space, subdiaphragmatic vessels, and diaphragm (p = 0.013 for the item of diaphragm, p < 0.001 for the other detailed items). Conclusion Although caution is required for general diagnostic purposes as image quality degrades, a camera-type device can be used to evaluate the inserted instruments in chest radiographs.

Characteristics of New Microsporidia S80 Isolated from Silkworm, Bombyx mori L. in Korea (가잠(家蠶)으로부터 분리(分離)된 새로운 Microsporidia S80의 특성(特性))

  • Lim, Jong Sung;Cho, Sae Yun
    • Current Research on Agriculture and Life Sciences
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    • v.1
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    • pp.67-83
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    • 1983
  • The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured $2.9{\pm}0.28{\mu}$ in length and $1.7{\pm}0.29{\mu}$ width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide ($H_2O_2$) at $30^{\circ}C$ was $26{\mu}$ of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of $60^{\circ}$ to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida, order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was $7.1{\times}10^7/ml$ as lower pathogenecity than that of Nosema bombycis Naegeli of $1.2{\times}10_7/ml$. While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell weight and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg batches from the infected female moth.

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