Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antibacterial and Antioxidant Potential of Methanol Extract of Viburnum sargentii Seeds

Viburnum sargentii 종자 메탄올 추출물의 항균 및 항산화 활성에 대한 연구

  • Patil, Maheshkumar Prakash (Research Institute for Basic Sciences, Pukyong National University) ;
  • Seong, Yeong-Ae (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Kang, Min-jae (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Singh, Alka Ashok (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Niyonizigiye, Irvine (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Kim, Gun-Do (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Lee, Jong-Kyu (Department of Physics, College of Natural Sciences, Pukyong National University)
  • Received : 2019.03.19
  • Accepted : 2019.06.05
  • Published : 2019.06.30
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Abstract

Antibacterial and antioxidant activities of plant sources have attracted a wide range of interest across the world over the last decade. This is due to the growing concern for safe and alternative sources of antibacterial and antioxidant agents. In this study, we focused on the antibacterial and antioxidant activities and the chemical composition of a methanol extract from Viburnum sargentii seeds. The chemical composition was determined by gas chromatography-mass spectroscopy (GC-MS), and the antibacterial activity was screened by a disc diffusion assay. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the microbroth dilution and spread plate method, respectively. The V. sargentii extract showed growth inhibition activity on all tested Gram-positive (Listeria monocytogenes, Staphylococcus aureus, and Staphylococcus saprophyticus) and Gram-negative (Escherichia coli, Pseudomonas putida, and Proteus vulgaris) pathogenic bacteria. The MIC and MBC ranged from 0.156~1.25 mg/ml for Gram-positive and 0.625~5.0 mg/ml for Gram-negative tested bacteria. The GC-MS results revealed the presence of several phytochemicals such as ${\beta}-sitosterol$ and vitamin E, which are known for their pharmacological applications. The antioxidant activities of V. sargentii extract were investigated by three different methods: the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, the reducing power assay, and the total antioxidant capacity assay. The results showed a concentration-dependent antioxidant potential for all three used methods. In sum, our findings suggest that the methanol extract of V. sargentii seeds has the potential to inhibit the growth of pathogenic bacteria and provide antioxidant compounds, making it therefore worthy of further investigation.

최근에 들어서 전세계적으로 식물 자원에서의 항균력 및 항산화력에 대한 관심이 광범위하게 증가하고 있다. 이는 사용되고 있는 항균제와 항산화제보다 안전한 대체 자원에 대한 관심이 높아졌기 때문으로 볼 수 있다. 따라서 본 연구에서는 Viburnum sargentii 씨앗의 메탄올 추출물의 항균력 및 항산화력과 화학적 구성에 대하여 연구를 진행하였다. 추출물의 화학 조성은 가스 크로마토 그래피-질량 분석법으로 측정하였고, 디스크 확산 분석법을 이용하여 항균 활성을 알아보았다. 액체 배지 감수성 실험을 통해 최소 억제 농도(MIC)를 측정하였고, 한천 희석법을 통해 최소 살균 농도(MBC)를 측정하였다. V. sargentii 메탄올 추출물은 실험에 사용된 그람 양성균(Listeria monocytogenes, Staphylococcus aureus, Staphylococcus saprophyticus) 및 그람 음성균(Escherichia coli, Pseudomonas putida, Proteus vulgaris)의 성장을 억제하였고, MIC와 MBC는 그람 양성균에서 0.156-1.25 mg/ml, 그람 음성균에서는 0.625-5.0 mg/ml의 범위를 나타내었다. 가스 크로마토 그래피-질량 분석법 결과에서는 V. sargentii 메탄올 추출물이 약리 활성을 가지는 ${\beta}-sitosterol$과 vitamin E와 같은 여러 파이토케미컬을 포함하고 있는 것을 확인하였다. V. sargentii 메탄올 추출물의 항산화 활성은 DPPH free radical scavenging assay, reducing power assay, total antioxidant capacity의 세 가지의 실험 방법을 통하여 확인하였으며, V. sargentii 메탄올 추출물의 농도가 증가함에 따라 항산화 활성도 증가하는 것을 세 가지 실험에서 모두 확인하였다. 이러한 결과를 통하여 V. sargentii 씨앗이 병원성 균의 성장을 억제할 수 있는 활성을 가질 뿐만 아니라, 항산화 활성을 가지는 구성물이 풍부함으로 추가적인 연구를 진행하기에도 충분한 가치가 있는 것을 확인하였다.

Keywords

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Fig. 1. Antibacterial activity of Viburnum sargentii seeds methanol-extract, ampicillin and kanamycin against pathogenic bacteria measured on agar medium by disc diffusion method. All values presented as mean of triplicates and error bars indicates ± standard deviation.

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Fig. 2. Histograms indicates antioxidant activity of methanol extract from seeds of Viburnum sargentii by (A) DPPH free radical scavenging assay, (B) Reducing power assay, and (C) Total antioxidant capacity. All results are presented as a mean ± Standard deviation (n=3).

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Fig. 3. GC-MS chromatogram of the methanol extract of Viburnum sargentii seeds.

Table 1. Antibacterial activity of Viburnum sargentii seeds methanol extract

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Table 2 GC-MS data for the methanol soluble fractions of Viburnum sargentii (seeds extract)

SMGHBM_2019_v29n6_671_t0002.png 이미지

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