• Title/Summary/Keyword: Dilution test

Search Result 393, Processing Time 0.024 seconds

Antibacterial Activity and Synergism of Hydnocarpi Semen Extracts with Ampicillin or Oxacillin against Methicillin-resistant Staphylococcus aureus

  • Joung, Dae Ki;Shin, Dong Young;Kwon, Dong Yeul;Shin, Dong Won
    • Korean Journal of Plant Resources
    • /
    • v.29 no.6
    • /
    • pp.699-703
    • /
    • 2016
  • Methicillin-resistant Staphylococcus aureus (MRSA) is a serious clinical and an urgent problem worldwide. Few new drugs are available against MRSA, because MRSA has the ability to acquire resistance to most antibiotics, which consequently increases the cost of medication. In the present study, the antibacterial activity of Hydnocarpi Semen was investigated. The most effective method is to develop antibiotics from the natural products without having any toxic or side effects. Therefore, there is a need to develop alternative antibacterial drugs for the treatment of infectious diseases. Five Clinical isolates (MRSA) were obtained from five different patients at Wonkwang University Hospital (Iksan, South Korea). The Other 2 strains were ATCC 33591 (Methicillin-resistant strain) and ATCC 25923 (Methicillin-susceptible strain). Antibacterial activity (Minimal Inhibitory Concentrations, MICs) was determined by broth dilution method, disk diffusion method, MTT test, and checkerboard dilution test. Antibacterial activity of n-hexane fraction was remarkable, and had a MICs ranging from $31.25-125{\mu}g/m{\ell}$. FICI values for HFH+AM and HFH+OX were 0.13-0.19 and 0.04-0.29, showing the increase of synergistic effect. When combined together, these antibacterial effects were dramatically increased.

A Study on Agricultural the Treatment of Organic Phosphorous Agricultural Pesticides Wastewater by the Activated Sludge Process (활성슬러지공정에 의한 유기인계 농약폐수처리)

  • 최택열;최규철
    • Journal of Environmental Health Sciences
    • /
    • v.21 no.2
    • /
    • pp.36-41
    • /
    • 1995
  • In order to treat effectively various type of wastewater discharged from the manufacture factory of organic phosphorous agricultural pesticides by the activated sludge process. The acclimation test of sludge was carried out by the dilution of completely mixed raw wastewater. The results of experiment were obstained as follows. 1. The moderate dilution rate of mixed raw wastewater was founded to about 30 times. 2. The available range of BOD-SS loading was 0.1~0.15 $kg\cdot BOD/kg\cdot SS\cdot day$ and it was similar to the case of extended aeration activated sludge process. 3. After the acclimation of activated sludge, the concentration of MLSS was 2000 mg/L, removal efficiency of BOD more than 90%, and SVI 100, respectively. 4. The oxygen respiratory rate of acclimated sludge was $47 mg\cdot O_2/g\cdot hr$ and this was increased about 5 times than $10 mg\cdot O_2/g\cdot hr$ of ordinary sludge.

  • PDF

A Study on the Akabane disease antibody in Chung Buk-Do (충청북도 북부지방의 소 Akabane병 중화항체가 분포조사)

  • 최해연;정운선
    • Korean Journal of Veterinary Service
    • /
    • v.14 no.2
    • /
    • pp.154-158
    • /
    • 1991
  • To investigate the Akabane antibody in the cattle with the serological test in Chung Chung Buk Do from May to Nov 1191. The result are summarized as follows. 1. Breed in cattle reacted as positive condition in Akabane antibody 76 heads(42%) in 180 cattles reacted as positive condition in Akabane antibody, 23 heads(51%) in 45 Korea native cattle reacted as positive condition in Akabane antibody. 2. During 5, 9, 10, 11 month, Akabane antibody in cattle is over 45%. 3. Less of 2 years old and over 4 years old cattle are Akabane antibody in cattle is over 40%. 4. The relation of titer of 2 folds of dilution HA and 10 folds of dilution TCID$_{50}$ was same relation.n.

  • PDF

An Analysis of Characteristics of Particulate Matter Exhausted from Diesel Locomotive Engines (디젤기관차 엔진에서 배출되는 입자의 특성분석)

  • 박덕신;김태오;김동술
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.19 no.2
    • /
    • pp.133-143
    • /
    • 2003
  • Numerous evidence have been reported that fine particulate matters can play an important role in threatening human health. Recently concerns on fine particle pollution from various engines may require re-examination of particulate emission standards. The particles emitted by most diesel engines are mainly divided into their size ranges such as Dp< 50 nm and 50 nm< Dp< 1,000 nm. In this work, the number concentration and the size distribution of fine particles emitted from an exhaust manifold of a railroad diesel engine were measured under load test conditions using a scanning mobility particle sizer (SMPS). The fine particles observed were within the range of 7 to 304 nm under different load conditions with two different dilution ratios. The fine particles exhibited unique patterns showing bimodal shapes in size distribution.

Tribological Behaviors of DLC Thin Films Deposited using Precursor Gas diluted by Hydrogen under Aqueous Environment (수중 환경에서 수소로 희석된 반응 가스를 이용하여 증착된 DLC 박막의 트라이볼로지 거동)

  • Lee, Jin-U;Mun, Myeong-Un;Lee, Gwang-Ryeol
    • Proceedings of the Korean Institute of Surface Engineering Conference
    • /
    • 2012.05a
    • /
    • pp.338-339
    • /
    • 2012
  • This study examined the friction and wear behavior of diamond-like carbon (DLC) films deposited from a radio frequency glow discharge using a hydrogen diluted benzene gas mixture. The DLC films were deposited on Si (100) and polished stainless steel substrates by r.f.-PACVD at hydrogen to benzene ratios, or the hydrogen dilution ratio, ranging from 0 to 2.0. The wear test was carried out in both ambient and aqueous environments using a home-made ball-on-disk type wear rig. The stability of the DLC coating in an aqueous environment was improved by diluting the benzene precursor gas with hydrogen, suggesting that hydrogen dilution during the deposition of DLC films suppress the initiation of defects in the film and improved the adhesion of the coating to the interface.

  • PDF

Quantitative Analysis of Trace pp'-DDE in Corn Oil by Isotope Dilution Mass Spectrometry : Uncertainty Evaluations

  • 김병주;김달호;최종오;소헌영
    • Bulletin of the Korean Chemical Society
    • /
    • v.20 no.8
    • /
    • pp.910-916
    • /
    • 1999
  • A current interest in chemistry concerns traceability of analytical measurements to the International System of Units (SI) and the proper estimation of their uncertainties in accordance with the internationally agreed guide provided by the International Organization for Standardization (ISO). Isotope dilution mass spectrometry (IDMS) is regarded as a primary method, which make the measurement results traceable to SI units without significant empirical correction factors. Our laboratory, as the national standards institute of Korea, participated in an intercomparison of environmental analysis, pp'-DDE in corn oil, which was organized by the CCQM under supervision of the CIPM to test feasibility of IDMS as a primary method for the trace analysis of organic compounds. In this report, we provide basic equations used for the calculation of the concentration of the analyte in a sample and a precise description of the processes for the evaluation of the uncertainties of the measurement results. Also, we report the experimental conditions adopted to improve the accuracy of the IDMS measurement. The principles contained in ??Guide to the Expression of Uncertainty in Measurement'' provided by ISO are followed for the uncertainty evaluation.

시추공 지구물리 방법에 의한 결정질암의 대수 특성 정밀

  • Kim Yeong-Hwa;Gong Nam-Yeong;Lee Seong-Jin
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
    • /
    • 2006.04a
    • /
    • pp.92-96
    • /
    • 2006
  • Borehole geophysical techniques were adopted for characterizing aquifer properties of crystalline rock formation in Bongmyong borehole test site, Kangwon National University. Specific fractures and/or fracture zones accompanying groundwater flow were delineated well by high resolution flowmeter and dilution measurements, and could be confirmed fromconventional well log methods. These geophysical techniques have been proved to be the most effective in aquifer characterization in crystalline rock formation.

  • PDF

Comparative Antimicrobial Susceptibility by the Methods of Test (검사방법(檢査方法)에 따르는 항균제감수성(抗菌劑感受性)의 비교(比較))

  • Park, Dong-Choon;Chun, Do-Ki
    • The Journal of the Korean Society for Microbiology
    • /
    • v.13 no.1
    • /
    • pp.43-48
    • /
    • 1978
  • The difference in antimicrobial susceptibility of multiply drug-resistant Salmonella typhi by the methods of test was tested against chloramphenicol(Cm), tetracycline(Tc), ampicillin(Ap), kanamycin(Km), and rifampicin(Rif), and the results were compared by the minimum inhibitory concentration(MIC). No appreciable difference was noted between MICs of Cm, Tc, and Rif measured by agar plate dilution(plate) method and broth dilution(broth) method. However, MICs of Km to about a half of test strains were 4 to 8-fold higher by broth method than plate method, and MICs of Ap by broth method were also a little higher than plate method in some strains. Cm and Rif were bacteriostatic rather than bactericidal to the most of test strains in high concentrations. Tc, Ap and Km were exclusively bactericidal to the test strains.

  • PDF

Biocidal effect to fish pathogens of Aqua farmsafe® composed of yucca extract and didecyldimethylammonium chloride (유카추출물과 didecyldimethylammonium chloride를 주성분으로 하는 살균소독제 아쿠아 팜세이프의 어류병원체에 대한 살균 효과)

  • Seo, Jung Soo;Jeon, Eun Ji;Hwang, Jee Youn;Jung, Sung Hee;Park, Myoung Ae;Lee, Sung Min;Lee, Eun Hye
    • Journal of fish pathology
    • /
    • v.26 no.2
    • /
    • pp.111-116
    • /
    • 2013
  • In this study, the disinfectant efficacy of Aqua farmsafe$^{(R)}$, composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe$^{(R)}$ possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe$^{(R)}$ against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua farmsafe$^{(R)}$ will be able to reduce fish diseases.

A Serological Test for Syphilis among Prostitutes by VDRL Slide Test (VDRL Slide Test에 의한 접대부의 매독혈청검사 성적)

  • 김영의;김경준
    • Journal of Environmental Health Sciences
    • /
    • v.1 no.1
    • /
    • pp.18-20
    • /
    • 1974
  • A Serological test for Syphilis by VDRL Slide tests were made to find the degree of treponemal infection. For this study sera were collected from 5, 312 Prostitutes of Seoul, Inchun, Euijungbu, Yangzoo, Pajoo and Pyungtaig from November 27, to December 7, 1972 and were examined on the following items. 1. VDRL Slide Qualitative test. 2. VDRL Slide Quantitative test. As the results of this study the following Conclusions were obtained. 1) This test were obtained average reactive 5.92% for 5, 312 prostitutes in Seoul 7.87% for 254 prostitutes, in Iuchun 6.77% for 354 prostitutes, in Euijungbu 6.62% for 740 prostitutes, in Yangjoo 5.82% for 1, 958 prostitutes, in Pajoo 6.08% for 937 prostitutes and in Pyungtaig 4.82% for 1, 069 prostitutes respectively 2) The highest Reagin titer was 1: 32 serum dilution.

  • PDF