• 대한본초학회지
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• 제22권4호
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• pp.29-34
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• 2007

Objective : Kyenegum(Galli Stomachichum Corium) has been popularly used long as the digestive. The purpose of this study was to investigate the enzymatic characteristic of Kyenegum crude enzyme. Methods : To evaluate of the enzymatic characteristic of Kyenegum, we examined the activity of Kyenegum crude enzyme from optimum solvent, optimum temperature and pH of crude Kyenegum extract. Futhermore, we examined the effects of NaCI and acidity of crude Kyenegum extract. Results : The Kyenegum was composed with crude protein about 20%, crude lipid 2%. The optimum Kyenegum dry condition, optimum extract solvent, optimum temperature and optimum pH were $4{\sim}6$ hours at $60^{\circ}C$, commercial apple vinegar, $50^{\circ}C$ and 2.0. Conclusion : The result suggests that the Kyenegum crude enzyme extract very strong enzyme in temperature, NaCl and acidity, respectively.

• 한국양식학회지
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• 제9권2호
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• pp.167-168
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• 1996

Experiments were conducted to determine the activities of protease and carbohydrase in growing lensky sturgeon fed with three different diets containing various concentrations of protein and carbohydrate. Neutral pretense activity from growing lensky sturgeon the protein diet (predo-minary, almost $100\%$ protein) was lower then those from fish fed the other diets during the experimental period. The results may indicate that the level of pretense activity is inversely related to the level of protein in the diets.

• 한국식품영양과학회지
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• 제43권11호
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• pp.1701-1708
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• 2014

참취의 활용 및 생리활성을 증가시킬 수 있는 적정 추출방법을 알아보고자 상온교반, 환류냉각, 가압가열, 저온고압 및 초음파 추출법을 이용하여 추출한 두벌 째 수확 참취 추출물의 생리활성을 비교하였다. 추출 수율은 환류냉각추출, 초음파추출, 가압가열추출, 상온추출, 저온고압추출 순으로 높았다. 폴리페놀, 플라보노이드 함량은 저온고압추출에서 유의적으로 높았고, 상압가열, 고온고압, 초음파추출에서는 대등한 함량을 나타내었다. 페놀화합물 정량의 경우 chlorogenic acid 함량은 가압가열추출에서 유의적으로 높았으며 cynarin 함량의 경우 환류냉각, 저온고압 및 초음파 추출에서 유의적으로 높았고, astragalin 함량의 경우 저온고압추출에서 높은 함량이 검출되었다. Xanthine oxidase(XO), angiotensin I-converting enzyme(ACE), HMG-CoA reductase 저해활성의 경우 저온고압추출 및 초음파 추출물에서 우수한 활성을 나타내었으며, 소화효소 저해활성(${\alpha}$-amylase, trypsin, lipase)에서도 유사한 경향을 나타내었다. 이러한 결과를 종합해 볼 때 저온고압 및 초음파 추출물에서 소재 활용가치가 높을 것으로 사료되며 천연 항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다.

• 한국어류학회지
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• 제21권3호
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• pp.149-157
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• 2009

말쥐치 자치어의 성장에 따른 소화기관 및 trypsin, pepsine-like enzyme, lipase, amylase 활성의 변화를 확인하기 위하여 64일간 사육실험을 수행하였다. 자어는 부화 후 2~26일 rotifer Brachionus plicatilis를 급이 하였고 부화 후 10~64일에는 Artemia nauplii를 급이 하였다. 그리고 부화 후 40일부터 배합사료를 급이 하기 시작하여 사료를 서서히 전환하였다. 사육기간 동안 수온은 $21.5{\sim}24.2^{\circ}C$를 유지하였다. Trypsin과 Lipase 활성은 부화 후 4일째($6.0{\pm}1.4unit$) 그리고 6일째($4.5{\pm}1.4unit$) 각각 확인되었다. 이들 두 효소의 활성은 말쥐치 자어후기와 치어초기에 급격히 증가하는 경향을 나타내었다. Pepsin-like enzyme 활성은 부화 후 10일째 확인되었고 말쥐치 치어 초기 단계인 28일부터 급격히 증가하였다. Pepsin-like enzyme 활성 변화와 위선 수의 변화가 일치하는 것은 흥미로운 현상이라고 할 수 있다. Amylase 활성은 부화 후 10일째 확인되었고 부화 후 28일까지 낮은 값을 유지하였으며 부화 후 28일과 40일 사이에 급격히 증가하였다. 결과적으로 말쥐치 치어초기의 Trypsin과 Pepsin-like enzyme 활성의 급격한 증가와 위선 수의 증가에 기인하여 치어기 급격한 체성장이 일어났을 것으로 사료된다.

• Journal of Pharmaceutical Investigation
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• 제18권3호
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• pp.125-131
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• 1988

This study was undertaken to investigate the proteolytic enzyme from Neungee mushroom [Sarcodon aspratus (Berk) S. Ito]. The proteolytic activity of Neungee was higher than other several edible mushrooms under various pHs. The potency of proteolytic enzyme of Neungee was same as the digestive drugs containing protease. So the proteolytic activity of the enzyme was increased in neutral or weak alkaline pH, whose characteristics would be alkaline protease. The specific activity of the purified enzyme obtained by using Tris acryl CM-cellulose ion exchange increased 20 times as compared with that of the crude extract. The proteolytic enzyme was stable at room temperature, but decomposition was fast when incubated at higher temperature more than $40^{\circ}C$. The half life of the enzyme was longest in neutral pH and rate constant was increased in acidic or alkaline solution.

• Bulletin of the Korean Chemical Society
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• 제33권3호
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• pp.925-928
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• 2012

The accurate expression for the steady-state velocity of an irreversible enzyme-catalyzed reaction obtained by Shin and co-workers (J. Chem. Phys. 2001, 115, 1455) is generalized to allow for the rebinding of the product. The amplitude of the power-law ($t^{-1/2}$) relaxation of the free- and bound-enzyme concentrations to steady-state values is expressed in terms of the steady-state velocity and the intrinsic (chemical) rate constants. This result is conjectured to be exact, even though our expression for the steady-state velocity in terms of microscopic parameters is only approximate.

• 대한한방내과학회지
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• 제11권2호
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• pp.148-169
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• 1990

The Present experiment was designed to investigate the effects of Soo Jeom San on the function of heart and digestive organs. And thus it was analyzed the total acidity, recovery effect, and the other various enzyme activities such as ATPase, Creatine kinase, Aspartate transaminase, and Lactate dehydrogenase. The results were obtained as follows : 1. The Total acidity decreased after Soo JeomSan administration for 6 days, however the total acidity inoreased after the drug administration for 9 days, these phenomena demonstrate that Soo Jeom San acts as a dual factor. The mechanism of decreasing the total acidity was considered to the inhibition of ATPase activity used for HCI active transport from parietal cells. 2. Soo Jeom San recovered the islets of Langerhans which was disrupted by streptozotocin. The recovery mechanism was suggested that Soo Jeom San stimulates the ${\beta}-cell$ proliferation. 3. Soo Jeom San inhibited the enzyme activities such as Creatine kinase and Aspartate transaminase, however the drug activated Lactate dehydrogenase. According to the obtained results, Soo Jeom San may be used for curing gastric ulcer and myocardiac infarction.

• Toxicological Research
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• 제20권2호
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• pp.167-172
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• 2004

Obesity is one of causes of the all adult diseases. We investigated the body weight decrease effect of the selected plants by digestive enzyme activity inhibition test. In a preliminary test, Inonotus obliquus and Rubus coreanus Miq. were found to be effective. Based on this result & previous result, we manufactured the mixture of plant extracts named as Misol$^{TM}$. Misol is applied anti-obesity beverage and rice. When we administered Miso/ sub TM/ to rats, it was found to be effective in body weight decrease. Result that is experimented during 20 weeks, is effective body weight and total cholesterol, triglyceride decrease. In case of human, anti-obesity beverage administered group showed 5.65% body weight decrease, 4% waist length decrease and 6% abdomen length decrease respectively. And anti-obesity rice administered group showed 2.3 kg body weight decrease, 2.9 cm waist length decrease and 3.74 cm abdomen length decrease respectively. This body control effect was supported by plant extracts in the Misol, anti-obesity beverage and rice - administered group. From these results, we suggest that this functional food could be helpful for body weight controlling obesity.

• 한국수산과학회지
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• 제56권5호
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• pp.634-643
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• 2023

The effects of dietary supplementation with fermented blood meal (FBM) in two forms were evaluated on the growth performance, hematological parameters, innate immune response, antioxidant capacity, digestive enzyme activity and intestinal histology of Japanese eel Anguilla japonica. A commercial powder feed was used as a basal diet (Con). Six other diets were prepared by supplementing 2, 4 and 6% FBM either in powder or liquid form to the Con diet (designated as P2, P4, P6, L2, L4 and L6, respectively). In total 420 eels (initial mean body weight: 55.1±1.71 g) were distributed into 21 tanks (450L) at a density of 20 eels per tank. Three replicate groups of fish were fed one of the seven diets twice daily for 12 weeks. No significant differences were observed in the growth performance and survival among all groups. Fish fed FBM-containing diets showed significantly improved hematological parameters, innate immunity and antioxidant capacity than those in the Con group. Fish fed FBM-containing diets showed significantly enhanced digestive enzyme activity, villus length and goblet cells than those in fish fed Con diet. These results indicate that both powder and liquid FBM could be a good functional feed additive to improve innate immunity and digestion in Japanese eel.

• 한국해양생명과학회지
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• 제7권2호
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• pp.145-153
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• 2022

수은은 생물 축적과 먹이사슬을 통한 생물 농축되며, 미량에서도 유해한 영향을 나타내기 때문에 해양 환경 내에서 중요한 문제가 되고 있다. 그러나 해양 소형 갑각류에 대한 수은의 생물 영향은 다른 금속에 비해 연구가 미흡하다. 본 연구에서는 기수산 물벼룩 Diaphanosoma celebensis을 아치사 농도(0.2, 0.4, 0.8 ㎍/l)의 무기 수은(HgCl₂)에 48시간 노출시킨 후, 대사 관련 유전자의 발현 양상을 조사하였다. 해독효소 유전자 5종(cytochrome P450; cyp360A1, cyp361A1, cyp4AP3, cyp4C122, cyp370C5)과 소화효소 6종(alpha amylase (AMY), alpha amylase related protein (AMY-like), trypsin (TRYP), chymotrypsin-like protein (CHY), lipase (LIP), pancreatic lipase-related protein (PLRP))의 유전자 발현을 quantitative real time reverse transcription polymerase chain reaction (qRT-PCR)을 이용하여 분석하였다. Cyp 유전자의 경우 clan2에 속하는 cyp370C5와 clan4에 속하는 cyp4AP3 유전자의 발현이 농도 의존적으로 유의하게 증가하였다. 한편 소화효소 유전자 중에서는 단백질 소화와 관련 있는 TRYP 유전자의 발현이 농도 의존적으로 증가하였다. 이러한 결과는 cyp370C5와 cyp4AP3가 수은 독성을 해독하는 과정에서 중요한 역할을 담당할 것으로 보이며, 수은이 소화효소 유전자의 발현을 조절함으로써 에너지 대사에 영향을 미칠 수 있음을 제시한다. 본 연구는 해양 소형 갑각류에서 수은에 대한 분자 수준의 영향을 이해하는데 도움이 될 것이다.