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Developmental Changes in Digestive Organ and Digestive Enzyme Activity of Filefish Thamnaconus modestus  

Gwak, Woo-Seok (Marine Bio-Education & Research Center, The Institute of Marine Industry, Gyeongsang National University)
Lee, So-Gwang (Gyeongsangnam-do Fisheries Resources and Research Institute)
Publication Information
Korean Journal of Ichthyology / v.21, no.3, 2009 , pp. 149-157 More about this Journal
Abstract
Larvae and juveniles of the filefish Thamnaconus modestus were reared for 64 days after hatching (DAH) in order to determine the activity of four enzymes (trypsin, pepsin-like enzyme, lipase, amylase) during ontogeny. Larvae were fed on rotifer Brachionus plicatilis from 2 to 26 DAH, Artemia nauplii from 10 to 64 DAH, and then gradually changed to pelleted feed from 40 DAH. Temperature was kept between $21.5{\sim}24.2^{\circ}C$ Activity of trypsin and lipase was found in larvae 4 DAH ($6.0{\pm}1.4unit$) and 6 DAH ($4.5{\pm}1.4unit$), respectively. The evolution of activity in both enzymes showed a profile marked by drastic increases between late larval and early juvenile stages. Pepsin-like enzyme activity was found at 10 DAH and drastically increased from 28 DAH, corresponding with the early juvenile stage of T. modestus. Interestingly, developmental changes in the pepsin-like enzyme activity coincided well with increases in the number of gastric glands. Amylase activity was found at 10 DAH and was maintained at a low level up to 28 DAH, followed by a drastic increase from 28 DAH to 40 DAH. It might be concluded that a drastic increase in trypsin and pepsin-like enzyme activities, and a corresponding increase in the number of gastric glands reflects a higher somatic growth of T. modestus during the early juvenile period.
Keywords
Thamnaconus modestus; gastric gland; trypsin; pepsin-like enzyme; lipase; amylase; protein;
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Times Cited By KSCI : 2  (Citation Analysis)
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