• Title/Summary/Keyword: Differentiated others

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Safety Assessment of Liquid-Type Herbal Preparations (한약제제(액제)의 안전성 평가)

  • Jo, Hyun-Ye;Jeon, Jong-Sup;Kim, Beom-Ho;Cho, Sang-Hun;Park, Sin-Hee;Kim, Young-Sug;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.26 no.3
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    • pp.266-272
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    • 2011
  • The safety of liquid-type herbal preparations (47 samples) and beverages containing herbal medicines (18 samples), which are distributed in Gyeonggi province was studied by analyzing heavy metals, benzo(a)pyrene, preservatives and sugar content and to explain the differences between medicine and food based on the labeling standards. The herbal preparation is regarded as medicine and can be differentiated from the beverages containing herbal medicine, which is food, based on the fact that the herbal preparation should be labeled with usage, dosage, efficiency and effect. The mean concentrations of Pb, As, Cd, Hg were found to be 0.014 mg/kg, 0.113 mg/kg, 0.004 mg/kg and 0.003 mg/kg in herbal preparation and 0.009 mg/kg, 0.122 mg/kg, 0.003 mg/kg and 0.002 mg/kg in beverages containing herbal medicines, respectively. In 10 cases of herbal preparations and 6 cases of beverages containing Rehmanniae Radix Preparata (Sukjihwang), benzo(a)pyrene was not found. According to preservative test on herbal medicine, dehydroacetic acid was found less than the indicated content in 6 cases out of 37 cases, which labeled with preservatives, and benzoic acid, dehydroacetic acid and methylparaben were contained with 164.0~198.0 mg/kg, 149.8~272.5 mg/kg and 88.4 mg/kg, respectively, in 3 cases out of 10 cases, which was not labeled with preservatives. Among 16 cases of beverages containing herbal medicines, 3 cases of preservative-labeled beverages had the preservatives, and the rest of all, which was not labeled with preservatives, did not show that they contain any preservative, therefore, it was suitable for the labeling standards. As a result of sugar content test, preservative-labeled products were not appreciably different from the others.

Effect of combinatorial bone morphogenetic protein 2 and bone morphogenetic protein 7 gene delivery on osteoblastic differentiation

  • Bae, Young;Kim, Kyoung-Hwa;Kim, Su-Hwan;Lee, Chul-Woo;Koo, Ki-Tae;Kim, Tae-Il;Seol, Yang-Jo;Ku, Young;Rhyu, In-Chul;Chung, Chong-Pyoung;Lee, Yong-Moo
    • Journal of Periodontal and Implant Science
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    • v.39 no.sup2
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    • pp.279-286
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    • 2009
  • Purpose: Gene therapy (ex vivo) has recently been used as a means of delivering bone morphogenetic proteins (BMPs) to sites of tissue regeneration. In the present study, we investigated the effect of co-transduction of adenoviruses expressing BMP-2 and BMP-7 on osteogenesisof C2C12 cells in vitro. Methods: A replication-defective human adenovirus 5 (Ad5) containing a cDNA for BMPs in the E1 region of the virus (Ad5BMP-2 and Ad5BMP-7) was constructed by in vivo homologous recombination. Functional activity of Ad5BMP-2 and Ad5BMP-7 were evaluated in mouse stromal cells (W20-17cells). C2C12 cells are transduced with various MOI (multiplicity of infection) of Ad5BMP-2 and Ad5BMP-7 to assess most effective and stable titer. Based on this result, C2C12 cells were transduced with Ad5BMP-2 and Ad5BMP-7 alone or by combination. BMPs expression, alkaline phosphatase (ALPase) activity, cell proliferation, and mineralization were assessed. Results: Ad5BMP-2 and Ad5BMP-7 are successfully transduced to W20-17 cells, and secreted BMPs stimulated cell differentiation. Also, C2C12 cells transduced with Ad5BMPs showed expression of BMPs and increased ALPaseactivity. In all groups, cell proliferation was observed over times. At 7days, cells co-transduced with Ad5BMP-2 and Ad5BMP-7 showed lower proliferation than the others. C2C12 cells co-transduced with Ad5BMP-2 and Ad5BMP-7 had greater ALPaseactivity than that would be predicted if effect of individual Ad5BMPs were additive. Little mineralized nodule formation was detected in cells transduced with individual Ad5BMPs. In contrast, Ad5BMP-2 and Ad5BMP-7 combination stimulated mineralization after culturing for 10 days in mineralizing medium. Conclusions: Present study demonstrated that adenoviruses expressing BMPs gene successfully produced BMPs protein and these BMPs stimulated cells to be differentiated into osteoblastic cells. In addition, the osteogenic activity of Ad5BMPs can be synergistically increased by co-transduction of cells with Ad5BMP-2 and Ad5BMP-7.

Cultural Politics of Gendered Schadenfreude Surrounding an Idol Focusing on the debate over IU (아이돌을 둘러싼 젠더화된 샤덴프로이데(Schadenfreude)의 문화정치학 <아이유 사태>를 중심으로)

  • Kim, Hyun Gyung
    • Korean journal of communication and information
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    • v.80
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    • pp.115-142
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    • 2016
  • This study aims to reveal the content of and logic behind a recent negative public sentiment toward female idols with the example of a debate over songstress IU's fourth album that was released late last year. While previous studies on fandom have focused on the identification process towards entertainers and making community, a recent phenomenon of "anti-fandom" or "malicious comments" implies that more research is needed on negative emotions such as hostility or schadenfreude (feelings of pleasure from others' misfortunes). Schadenfreude is a social sentiment that originated in modern liberalism, which features contradictions between public equality and private ownership, and that has been intensified in neoliberalism, which features a maximization of this contradiction centering on a meritocracy. Celebrities in Korea often become the targets of schadenfreude, which is associated with the suspicion that they gain popularity not from their abilities but from "just being popular." It should also be noted that this kind of schadenfreude operates differently between male and female entertainers. Specifically, the acquisition of money and fame by modern women whose presence used to be located in the private possessions of males is considered to be due to their unjustified use of sexuality. This is also the background of the recent online misogyny culture in Korea. In this context, IU, who had been successful at building a differentiated image of "sister-like idol artist," became a valid target. Although accusing IU of utilizing pedophilia reflects a stalemate that a current politics of sexual violence faces, it rather damages the name of an individual than attracts public attention to the structural causes of childsexualabuse. This is why I see the way that pedophilia was used in the debate over IU as a schadenfreude. Consequently, the term pedophilia here contributes to an expansion of the entertainment economy that is sustained by rises and falls of the celebrities' stock prices.

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What Factors Affect Pre-service Teachers in Choosing Teaching Science as Career?: Teaching Motivations of Pre-Service Science Teachers in Korea (왜 과학교사가 되려하는가? -우리나라 예비과학교사들의 교직 선택 동기-)

  • Lee, Bongwoo;Kim, Heekyong
    • Journal of The Korean Association For Science Education
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    • v.38 no.2
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    • pp.123-133
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    • 2018
  • The purpose of this study is to investigate pre-service science teachers' motivation factor and satisfaction of teaching choice in Korea. For this purpose, we surveyed 815 pre-service science teachers in 11 universities in Korea. The questionnaire used in this study was the FIT-Choice scale (Factors Influencing Teaching-Choice) developed by Richardson and Watt (2006). The most significant factor in choosing teaching science as a profession was 'intrinsic value,' followed by 'social utility value,' but lower than the average of other countries in the previous research results. The confidence in teaching professions was low because the percentage of respondents about 'ability' was low and 'task demand' was high. Satisfaction with teaching choice was 5.36, and it is lower than the average of responses in Australia and USA. As for the differences according to gender, male pre-service teachers responded that they chose teaching because they have the proper ability to teach, while female pre-service teachers perceived that the influence of others was greater. Also, female pre-service teachers felt the burden of job such as hard work and emotional demands more than men. Finally, the motivation which showed the highest correlation with the satisfaction of choosing to teach was the intrinsic value. Therefore, finding ways to increase the intrinsic/social utility motivation for pre-service science teachers and differentiated approach considering gender differences are needed.

Chemical Compositions of Glass Beads from Tombs of Bupwha-ri Site, Yeongdong (영동 법화리유적 출토 유리구슬의 화학적 특성: 토광묘 6호 및 8호)

  • Chung, Kwang-Yong;Kang, Hyung-Tae;Koh, Min-Jeong;Kim, Hwa-Jung
    • Journal of Conservation Science
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    • v.27 no.3
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    • pp.243-250
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    • 2011
  • Seven glass beads from Bupwha-ri site, Yeongdong dated Joseon dynasty were analyzed with SEM/EDS and X-ray diffraction methods. Six samples were classified to potash glass system($K_2O-CaO-SiO_2$) with HCA(High CaO and $Al_2O_3$) and high concentration of MgO suggested raw materials to plant ashes. Especially one sample from tomb no. 8 was highly different concentration of $K_2O$ and MgO with others. It was shown that they were different sources of raw materials. Mostly coloring agents of potash glasses were owing to Fe and Cu. One sample was found to lead glass system(PbO-$SiO_2$) with 12% PbO. The content of PbO was differentiated with other lead glasses since we analyzed so far. It needs more study for compositions of lead glasses with regions and ages.

Effects of Road and Traffic Characteristics on Roadside Air Pollution (도로환경요인이 도로변 대기오염에 미치는 영향분석)

  • Jo, Hye-Jin;Choe, Dong-Yong
    • Journal of Korean Society of Transportation
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    • v.27 no.6
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    • pp.139-146
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    • 2009
  • While air pollutants emission caused by the traffic is one of the major sources, few researches have done. This study investigated the extent to which traffic and road related characteristics such as traffic volumes, speeds and road weather data including wind speed, temperature and humidity, as well as the road geometry affect the air pollutant emission. We collected the real time air pollutant emission data from Seoul automatic stations and real time traffic volume counts as well as the road geometry. The regression air pollutant emission models were estimated. The results show followings. First, the more traffic volume increase, the more pollutant emission increase. The more vehicle speed increase, the more measurement quantity of pollutant decrease. Secondly, as the wind speed, temperature, and humidity increase, the amount of air pollutant is likely to decrease. Thirdly, the figure of intersections affects air pollutant emission. To verify the estimated models, we compared the estimates of the air pollutant emission with the real emission data. The result show the estimated results of Chunggae 4 station has the most reliable data compared with the others. This study is differentiated in the way the model used the real time air pollutant emission data and real time traffic data as well as the road geometry to explain the effects of the traffic and road characteristics on air quality.

Studies on the Differentiation of Chondrogenic Cells in Developing Chick Embryo I. Cellular Aggregation and Chondrogenesis (발생계배 연골세포의 분화기구에 대한 연구 I. 세포응집과 분화와의 관계)

  • 박대규;손종경;유정아;유병제;강신성
    • The Korean Journal of Zoology
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    • v.33 no.3
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    • pp.310-321
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    • 1990
  • To establish the in vitro culture system and quantitation for chondrogenesis, and to investigate the relationship between cell aggregation and chondrogenesis, chick limb bud mesenchymal cells of Hamburger-Hamilton stage 23/24 were micromass cultured in various cell densities. The chondrogenesis was assayed based on checking the alcian blue-stained nodule numbers, the amount of alcian blue extraded, the change in cell numbers, the rate of [35 S] sulfate incorporation and expression of type II collagen. Mesenchymal cells plated with an initial density of high (1 x 107 cells/ml)- and intermediates (5. $\times$ 106 cells/ml)-density were differentiated into cartilage. On the other hand, the cells of low density (2 x 106 cells/mi, 5 $\times$ 105 cells/ml) of stage 23/24 cells and the stage 18/19 cells in three kinds of cell density did not differentiate into cartilage even though the cells formed an aggregated core at the center of cultured mass. From these results and others obtained in this study, it can be stated that the stage 23/24 mesenchymal cells are likely to pass over the aggregation step and have the potentiality to differentiate into chondrocytes. Thus chondrogenesis in vitro can be observed when mesenchymal cells are plated over the threshold density of 5 $\times$ 106 cells/ml. Hyaluronidase (HAase) activity was relatively constant throughout the culture, suggesting that the role of HAase may not be important for the cells of stage 23/24.

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Studies on Bacterial Characteristics of Bacillus cereus Group LS-1 Isolated from Suyeong Bay (수영만에서 분리된 Bacillus cereus Group LS-1 의 세균학적 특성에 관한 연구)

  • 성희경;이원재;김용호;함건주
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.339-346
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    • 1992
  • These studies were carried out to identify Bacillus cereus group 1..5-] strain isolated from 5uyeong Bay. This strain was differentiated from B. cereus group using conventional, API system and fatty acid composition analysis. Colony characteristics were opague. mucoid, entire margin. convex. circular and non hemolysis on sheep blood agar plates, and were observed with central spore forming positive bacilli in a Gram stained preparation. and had no motility. The carbohydrates tested; glucose.maltose, and sucrose were assimilated but neither trehalose nor salicin were assimilated. This strain ultilized gelatin and was also inhibited by 6.5% NaCI. The results of biochemical examination were differented from B. cereus group LS-1 compared with others B. cereus group. The fatty acid composition contained major amounts of branched chain acids. iso $C_{15}$ and iso $C_{13}$ and the range of chain length was $C_{12}$ to C"$C_{17}$ and n$C_{15}$, acid was not detected. Automated fatty acid computer profile indicated "B. mycoides GC subgroup B of 0.312 similarity index." The results agreed with other research cases. On the other hand. A TB computer prolile index of API system (API 50 CHB & API 20E) identified" Doubtful profile of 99.7% B. firmus" . These results were presented with considerable discrepancies between API system and fatty acid analysis. With 67 biochemical characters. the similarity matrix of B. mycaides (KCTC 1033). B. thuringiensis (KCTC 1033). B. cereus (5-3) and B. mycoides (S-12) showed 42%. 42%. 59%, and 52%. respectively. Through the key tests and fatty acid analyses. we could notice the appearance of B. mycoides of the B. cereus group and this leads us to suspect the existence of a new biotype B. mycoides.

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Analysis of PDL Fibroblast Change During Mechanical Stimuli in the Rats (기계적 자극에 대한 백서 치주인대 섬유아세포의 변화)

  • Kim, Tae-Gyun;Kim, Chang-Sung;Cho, Kyoo-Sung;Chai, Jung-Kiu;Kim, Chong-Kwan;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • v.31 no.2
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    • pp.277-285
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    • 2001
  • Periodontal disease is characterized by inflammation and subsequent loss and/or damage to tooth-supporting tissues such as bone, cementum,and periodontal ligament. Periodontal ligament and cementum are the key tissues in the initial process of regeneration following periodontal disease. Therefore, studies on cementoblasts, which form cementum are emphasized. It is still unclear which cells cementoblast differentiate from. This study was conducted under the hypothesis that PDL fibroblast can differentiate into either cementoblast or osteoblast depending on the conditions of surrounding tissue. Clinically, with excessive traction force of orthodontic appliances or excessive occlusion hypercementosis is observed, and this has been confirmed histologically. Consequently, activation of cementoblast can be expected in rats when mechanical stimuli are given to PDL fibroblast. Therefore, the purpose of this article is to prove that PDL fibroblast differentiates into cementoblast in rats under mechanical stimuli using histologic and molecular methods. In this study, twenty rats were given hard diet. Ten of them were sacrificed after 1 week, and the others were sacrificed after two weeks. Slides were made from tooth specimen, and they were studied under the microscope. In addition, PDL fibroblast and cementum from the extracted teeth were analyzed with Northern blotting. In histologic examination, as time passed, PDL fibroblast migrated to the dentin side, differentiated into cementoblast, and formed new cementum. In Northern blotting, it was found that mRNA expression of cementoblast-specific proteins such as BSP, OC, OPN, and type I collagen were more prominent in rats sacrificed after 2 weeks of hard-diet than rats sacrificed after 1 week. From these findings we can conclude that PDL fibroblast can differentiate into cementoblast under mechanical stimuli. We think that 'Rat Models' used in this study will be beneficial to future studies regarding cementoblast.

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The Relationship between Gymnasium Selection and Training Adherence of Security Martial Arts Trainees (경호무도 수련생의 도장선택과 수련지속의 관계)

  • Song, Gyu-Geun;Lee, Ki-Se;Min, Jae-Ki
    • Korean Security Journal
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    • no.27
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    • pp.107-128
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    • 2011
  • The purpose of this study was to determine the relationship between gymnasium selection and training adherence of security martial arts trainees. To this aim, this study selected 8 gymnasiums in Gyounggi and Incheon province and sampled 220 people above the fourth grade in elementary school. Out of these, 14 cases were dropped due to insufficient answers or incomplete answers, and 206 qualified cases were finally adopted for this study. Data were analyzed using exploratory factor analysis, reliability analysis and frequency analysis, MANOVA, correlation analysis and multiple regression analysis with SPSS 18.0. The results of this study were as follows. First, there were significant differences in gymnasium selection factors(instructor background, training program, public relations) depending upon the trainee's sex. Second, there were significant differences in gymnasium selection factors(training program, main others, public relations) depending upon the trainee's school grade. Third, there were no significant differences in training adherence factors depending upon the trainee's sex and school grade. Fourth, there were positive correlations between instructor background, training program and public relations-factors among gymnasium selection and management program, external and social relations-factors among training adherence. Fifth, the partial sub-factors of gymnasium selection have influenced training adherence. Consequently, security martial arts managers and instructors need to establish marketing strategies suitable for sex and school grade to recruit new security martial arts trainees. It may be considered that security martial arts instructors should construct the differentiated management system for trainees and the specialized training program for lasting training of new or existing security martial arts trainees.

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