• Toxicological Research
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• 제27권1호
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• pp.43-48
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• 2011

Even though exposure to benzene has been linked to a variety of cancers including leukemia, the detailed molecular mechanisms relevant to benzene-induced carcinogenesis remain to be clearly elucidated. In this study, we evaluated the effects of benzene on differential gene expression in a leukemia cell line. The K562 leukemia cell line used in this study was cultured for 3 h with 10 mM benzene and RNA was extracted. To analyze the gene expression profiles, a 41,000 human whole genome chip was employed for cDNA microarray analysis. We initially identified 6,562 genes whose expression was altered by benzene treatment. Among these, 3,395 genes were upregulated and 3,167 genes were downregulated by more than 2-fold, respectively. The results of functional classification showed that the identified genes were involved in biological pathways including transcription, cell proliferation, the cell cycle, and apoptosis. These gene expression profiles should provide us with further insights into the molecular mechanisms underlying benzene-induced carcinogenesis, including leukemia.

• Bulletin of the Korean Chemical Society
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• 제11권3호
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• pp.200-205
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• 1990

Chemically modified electrodes(CMEs), based on 2-imino-1-cyclopentane-dithiocarboxylic acid (icdc) containing carbon paste, have been characterized using cyclic voltammetric techniques. Ag(I) was chemically deposited on the CMEs, and voltammograms were obtained with the electrode in a separate buffer solution. The CME surface can be regenerated with exposure to acid and reused for deposition. In 10 deposition/measurement/regenerate cycles, the linear response have been reproduced up to $1{\times}10^{-6}$ M in linear sweep voltammetry and 1${\times}$10-8 M in differential pulse voltammetry with relative standard deviation of 5.2% and 12.4%, respectiveiy. The sensitivity increased with deposition time and scanning rate, and detection limit was $1{\times}10^{-7}M\;and\;1{\times}10^{-9}M$ at 20 minutes deposition in the linear sweep voltammetry and differential pulse voltammetry, respectively. The presence of some metal ions does not influence the silver ion response. Satisfactory results were obtained for the analysis of the silver ion for a variety of reference materials without interference of Hg ion at the condition of pH = 5-6.

• Molecules and Cells
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• 제46권7호
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• pp.399-413
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• 2023

cAMP responsive element-binding protein (CREB) is one of the most intensively studied phosphorylation-dependent transcription factors that provide evolutionarily conserved mechanisms of differential gene expression in vertebrates and invertebrates. Many cellular protein kinases that function downstream of distinct cell surface receptors are responsible for the activation of CREB. Upon functional dimerization of the activated CREB to cis-acting cAMP responsive elements within the promoters of target genes, it facilitates signal-dependent gene expression. From the discovery of CREB, which is ubiquitously expressed, it has been proven to be involved in a variety of cellular processes that include cell proliferation, adaptation, survival, differentiation, and physiology, through the control of target gene expression. In this review, we highlight the essential roles of CREB proteins in the nervous system, the immune system, cancer development, hepatic physiology, and cardiovascular function and further discuss a wide range of CREB-associated diseases and molecular mechanisms underlying the pathogenesis of these diseases.

• 대한환경공학회지
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• 제22권7호
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• pp.1243-1252
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• 2000

휘발성 훈증제인 cis-와 trans-1,3-D의 각각에 대한 분해력 향상은 몇몇 연구자들에 의해 이루어져왔다. 본 연구는 cis-와 trans-1,3-D에 대해 서로 다른 속도에서 각각의 분해력 증진과 미생물과의 관련성을 조사한 것으로, 미생물이 휘발성을 갖는 독성 유기화합물의 분해를 향상시키는 데 관여하고 있음이 관찰되었다. 1,3-D로 야외(field)처리되어 적응되어 왔던 토양으로부터, 1,3-D의 분해가 확인된 토양시료를 채취하여 1,3-D를 분해할 수 있는 혼합 배양세균을 분리하였다. 이렇게 분리된 혼합 배양세균은 cis-1,3-D보다는 trans-1,3-D를 더 빨리 분해 시켰으나, 미생물 성장을 위한 탄소원으로 cis-와 trans-1,3-D만이 제공되었을 때는 분해가 일어나지 않는 반면, 적절한 2차 탄소원들이 존재할 때에는 cis-와 trans-1,3-D를 분해시켰다. 따라서, cis-와 trans-1,3-D의 분해는 공동대사과정(cometabolism)인 것으로 판단된다. 두 이성질체는, 토양여과액(soil leachate), tryptone, tryptophan, alanine이 포함된 시료가 2차 탄소원으로 제공되었을 때에는 분해가 이루어졌으나, 고온고압하에서 멸균시킨 토양추출액(soil extract), glucose, yeast extract 및 indole이 포함된 시료가 2차 탄소원으로 제공되었을 때는 두 이성질체 모두를 분해시키지 못했다. 상업용 훈증제로 이용되는 cis-와 trans-1,3-D를 다른 속도로 개별적으로 분해하는 혼합 미생물군은 형태학적인 구별방법에 의해 4개의 독립된 순수 colony로 구성되어 있는 것으로 관찰되었다.

• 보건행정학회지
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• 제15권1호
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• pp.1-29
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• 2005

This study examined the antecedents of psychological empowerment at hospital organizations, and also examined their differential effects among occupational groups within hospitals: doctors, nurses, engineers, and administrative workers. Various variables for multi-level factors were examined as antecedents: tenure, income, work centrality, and career goal as personal factors, job variety, job clarity, job significance, and job fitness as job factors, and security, reward justice, and organizational support as organizational factors. Data were collected from 8 national university hospitals, and 1,289 data were used for final analysis. For the whole groups, all antecedents except reward justice had significant effects on, and explained large amount of variance of empowerment. Results from the analysis for each occupational group showed that income, career goal, and job significance had significant effects on empowerment at all occupational groups, while reward justice had not at any groups. The effects of other variables depended on occupational groups. 1bis study found some important antecedents of empowerment which have been less considered in previous research: career goal, work centrality, security, and organizational support. The finding that differential effects of antecedents on empowerment by occupational groups suggests that group characteristics should be considered for studying empowerment. In this study, for example, personal factors rather than both job factors and organizational factors were more effective for empowerment in the engineering group whose job is relatively simple and clear, while job factors were most effective in other groups. The differential effects of antecedents on empowerment by occupational groups also have practical implications for improvement of empowerment at hospitals. For empowerment, personnel management efforts would be more required for administrative workers than other occupational groups, because they perceived least job clarity, job significance, job fitness among the groups, all of which were found to be important determinants of empowerment for them.

• Food Science and Biotechnology
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• 제16권1호
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• pp.99-103
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• 2007

The purpose of this study was to develop a simple detection method, possibly at the species-level, that allows for large-scale screening of bifidobacteria. Human fecal samples were plated on MRS-raffinose agar containing cysteine and neomycin sulfate, serving as selective pressure for bifidobacteria, and 0.003%(w/v) bromocresol green. All of the test strains grew well on this medium at $37{\pm}1^{\circ}C$, forming white colonies surrounded by yellow halos, which presented a sharp contrast against the green background. In this disc assay, the required incubation time to develop a yellowish zone varied with the species of Bifidobacterium that was tested, allowing for differential counts and easy identification at the species-level: 10-14 hr for B. bifidum, 20-22 hr for B. catenulatum and B. infantis. and 24-25 hr for B. longum and B. breve. No apparent color was observed for B. angulatum and B. adolescentis 28 hr after inoculation. To evaluate the results of pH indicator-based identification, individual isolates were subjected to a colony-PCR experiment with genus-specific primers. The amplified products from the isolates were in good accordance with those from the reference strains at a level of 95% agreement. These results suggest that the present method could be conveniently applied to cell counts, as well as to the preliminary identification of bifidobacteria from a variety of sample types including human feces, dairy products, and commercial probiotic supplements.

• 한국컴퓨터정보학회논문지
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• 제25권6호
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• pp.65-72
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• 2020

오늘날 사물 인터넷은 우리에게 편의를 제공하기 위해 가정, 산업 현장 및 병원을 포함한 많은 장소에서 사용된다. 다양한 장치가 네트워크에 연결됨에 따라 많은 서비스들이 실시간 데이터 수집, 저장 및 분석을 통해 새로운 가치를 창출하고 있다. 이처럼 많은 분야에서 IoT 장치 내의 센서 및 통신 기능을 활용하는 서비스 및 애플리케이션을 개발하고 있다. 예시로 산업 분야에서 Samsung과 LG는 자사의 IoT 애플리케이션을 통해 가전과 IoT 기기를 연결하여 스마트 홈을 구축하는 서비스를 제공하며, 의료 및 건강 분야에서 Samsung과 Xioami와 같은 기업들은 피트니스 워치 및 앱을 통해 심전도를 확인하거나 운동량을 기록, 관리한다. 위 같은 사례에서 스마트 홈을 구축하는 서비스의 경우에 수집한 데이터를 통해 해당 가정의 생활 패턴이나 출퇴근 여부 등의 민감정보를 유출할 수 있다. 또한 의료 데이터로 사용하기 위해 측정한 데이터를 통해 개인 정보와 질병의 존재와 같은 민감정보를 유출할 수 있다. 따라서 이를 보호하기 위해 해당 논문이 제안하는 방법에 따라 데이터를 수집, 배포한다면 데이터를 제공하는 사용자의 개인 정보 보호에 위협을 막을 수 있다. 이를 해결하기 위해 최근에는 프라이버시 보호 데이터 처리에 차분 프라이버시(DP)가 채택되어왔다. 따라서 DP를 기반으로 스마트워치 플랫폼에서 건강 데이터를 안전하게 수집할 수 있는 방법을 제안하며, 이를 통해 위와 같이 다양한 분야에서 프라이버시를 보호하는 환경에서의 데이터 수집 및 배포를 가능케 할 수 있다.

• BMB Reports
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• 제40권2호
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• pp.286-289
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• 2007

Despite considerable interest in the biologic processes of regeneration and stem cell activation, little is known about the genes involved in these transformative events. In a Hydra littoralis model of regeneration, we employed a rapid shotgun suppression subtractive hybridization strategy to identify genes that are uniquely expressed in regenerating tissue. With an adaptor-PCR based technique, 16 candidate transcripts were identified, 15 were confirmed unique to mRNA isolated from hydra undergoing regeneration. Of these, 6 were undescribed in GenBank and allied expressed sequence tag (EST) databases (GenBank + EMBL + DDBJ + PDB and the Hydra EST database). BLAST analysis of these sequences identified remarkably similar sequences in anonymous ESTs found in a wide variety of animal species.

• 디지털산업정보학회논문지
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• 제5권3호
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• pp.201-213
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• 2009

An Airport is important space and even stands for the country because diverse people from other countries use a variety of facilities in the gateway to the country. Therefore, It is urgent for those people to increase efficiency and convenience of its usage. But we have a problem with process of passenger and this paper with the object of finding the solution in case study. This paper focus on information system, especially Intelligent Airport Information System to streamline process of passenger. There are a few of countries adopted this system in process. Therefore, IIAC(Incheon International Airport Corporation) establishing u-Information HUB System. It is unique and differential characteristics to Facilitation and increase passenger's satisfaction. u-Information HUB system unified stakeholder's process of Facilitation in Air Traffic Sides. u-Information HUB system gets the highest state of efficiency to Facilitation. This Paper is attempt to find new effort

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.53-56
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• 2008

An ejector is a fluid machinery to be utilized for mixing fluids, maintaining vacuum, and transporting them. The Ejector is applied for a variety of industrial fields such as refrigerators and power plants. It is adopted to recycle anode off gas safely in 5kW Molten Carbonate Fuel Cell system of KEPRI(Korea Electric Power Research Institute). The ejector is placed at mixing point between the anode off gas and the cathode off gas or the fresh air. In this study, the entrainment ratio is measured according to the diametrical ratio of nozzle to throat. In addition, the performance curve of the ejector and the differential pressure in diffuser is observed.