• BMB Reports
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• v.41 no.3
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• pp.259-265
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• 2008

Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of the pI values of mature proteins. Fluorescence microscope analysis indicated that three PODs are secreted into the extracellular space. RT-PCR analysis revealed that POD genes have diverse expression patterns in a variety of plant tissues. Swpb4 was abundantly expressed in stem tissues, whereas the expression levels of swpb5 and swpa7 transcripts were high in fibrous and thick pigmented roots. Swpb4 and swpa7 showed abundant expression levels in suspension cultured cells. Three POD genes responded differently in the leaf and fibrous roots in response to a variety of stresses including dehydration, temperature stress, stress-associated chemicals, and pathogenic bacteria.

• Journal of the Korean Society for Advanced Composite Structures
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• v.1 no.3
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• pp.24-34
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• 2010

This paper analyzed the partial differential equations of laminated composite shells of revolution by using the finite difference method. The proof that numerical results are reasonable and accurate is obtained through converge ratio analysis and commercial program LUSAS for the structural analysis. The purpose of this study is to examine closely the engineering advantages and to analyze the structural behaviors of the anisotropic shells of revolution. Thus, the relevant reinforcement and most suitable arrangement of fiber to produce the highest strength are proposed through the numerical results according to a variety of parameter study. Namely, the distribution of displacements and stress resultants are analyzed according to the change of meridian's curvature, the ratio of height-width of shell, subtended angle, fiber angle, and so on. Using these distribution, the most suitable shell may be proposed to produce the highest strength. Also, the configuration of the entire laminated composite conical shells is analysed, and a variety of the design criterion of circular conical shell are proposed and studied in engineering view points.

• The Plant Pathology Journal
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• v.37 no.5
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• pp.476-488
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• 2021

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot for cruciferous vegetables worldwide, especially for the cole crops such as cabbage and cauliflower. Due to the lack of resistant cabbage cultivars, black rot has brought about considerable yield losses in recent years in China. Understanding of the pathogen features is a key step for disease prevention, however, the pathogen diversity, population structure, and virulence are largely unknown. In this study, we studied 50 Xcc strains including 39 Xcc isolates collected from cabbage in 20 regions across China, using multilocus sequence genotyping (MLST), repetitive DNA sequence-based PCR (rep-PCR), and pathogenicity tests. For MLST analysis, a total of 12 allelic profiles (AP) were generated, among which the largest AP was AP1 containing 32 strains. Further cluster analysis of rep-PCR divided all strains into 14 DNA groups, with the largest group DNA I comprising of 34 strains, most of which also belonged to AP1. Inoculation tests showed that the representative Xcc strains collected from diverse regions performed differential virulence against three brassica hosts compared with races 1 and 4. Interestingly, these results indicated that AP1/DNA I was not only the main pathotype in China, but also a novel group that differed from the previously reported type races in both genotype and virulence. To our knowledge, this is the first extensive genetic diversity survey for Xcc strains in China, which provides evidence for cabbage resistance breeding and opens the gate for further cabbage-Xcc interaction studies.

• Korean Journal of Agricultural Science
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• v.4 no.2
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• pp.199-214
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• 1977

These studies were conducted to obtain basic information of the rice blast resistance in comparision with blast occurrence patterns in the blast nursery test in Korea. One hupdred and twenty nine rice varieties including several blast differential varieties and 30 combinations of pedigree lines were tested at six different locations, Suweon, Chulweon, Chuncheon, Iri, Jinan, Imsil, and Milyang, in 1976~1977. The results obtained are summarized as follows: 1. When blast resistance was tested using three sets of differential varieties at six locations, prevalent races were different depending on the location and differential variety set tested. 2. There were regional differences in blast disease reation among Japonica rice varieties and among Indica${\times}$Japonica crosses. 3. At Suweon blast disease reactions of pedigree lines were different from that at Imsil. 4. Three varietal groups were made depending on the develpment of rice blast in the nursery test. 5. In general, highly resistant varieties to rice leaf blast were also resistant to neck blast, but there were exceptions.

• Research in Plant Disease
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• v.13 no.1
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• pp.45-49
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• 2007

Single spores were isolated from infected roots of Chinese cabbage with a typical clubroot symptom, collected from different Chinese cabbage cultivation areas in Korea. When the single spore isolates were inoculated on Chinese cabbage, radish, turnip, kale, leaf mustard and Williams' differential varieties, among 321 roots harvested two weeks after inoculation, a visual symptom was observed on only one root and light/uncommon symptoms were done on 70 roots. These 71 individuals were homogenized and used as inocula. These inocula caused generally higher pathogenicity than that of single spore. Finally 15 isolates, with enough growth for conducting further experiment, were selected. These 15 individuals were grouped four, seven, two and two into race 1, race 4, race 9 and race 11, respectively, using Williams' differential set. It was confirmed that race 4 were dominantly present in Korea. These 15 had been obtained from roots of Chinese cabbages, radishes and turnips inoculated with single resting spores and had shown pathogenicity to Laurentian and Wilhelmsburger belong to Rutabaga in Williams' differential variety set. Therefore, we assume that such characteristic pathotypes including race 4, especially, of P. brassicae showing strong pathogenicity to Chinese cabbage, radish and turnip may be dominant in Korea.

• Biomolecules & Therapeutics
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• v.16 no.2
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• pp.95-99
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• 2008

Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.

• Proceedings of the Korean Geotechical Society Conference
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• 2003.03a
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• pp.103-110
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• 2003

This study is as for shale that attract recently geotechnical engineer more and more. like sedimentary rocks and metamorpic rocks, shales have many problem with anisotropy for stength and deformation and they have many problems with quick weathering progress and differential weathering of alternate bedding. In foreign countries, many renowned schalors, like Jaeger(1960),McLamore,Gray(1967),Donath(1972),Nova(1980),Hoek&Brown(1980),Ranamurthy(1985), have already studied for a variety of characteristic and announced high level results of their studies. In domestic also, there are many scholars who have announced high level research papers for shale. this study is a part of these stream. and this study not only analyzed strength anisotropy characteristic along with direction of testing(two-direction) by using point-load test(log-log method) but also compared uniaxial strength between the maximum saturated and dried condition. In this study, we also conducted slaking test. these results of slaking test show weathering characteristic of shales. also, we made the most of field data that obtained during slope stability project and we noticed that RQD measured in the field is much differ from drilled core RQD. In order to come close two different value or access to reality, we suggest new RQD method that artificially reduce RQD by separating core with light hand force.

• Toxicological Research
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• v.19 no.3
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• pp.235-240
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• 2003

The modification of CYP2E1 activity is of considerable interest because of its role in the metabolic activation of a variety of toxic chemicals. In the present studies, the time-course of changes in human CYP2E1 activities was determined after treatment with ethanol, glycerol, 4-methylpyrazole or isoniazid using intact HepG2 cells transfected by human CYP2E1. Hydroxylation of chlorzoxazone was chosen for the measurement of CYP2E1 activity. CYP2E1 protein levels were increased upon cultivation of cells in the presence of ethanol, glycerol, 4-methylpyrazole or isoniazid for 24 hr. After 24 hr cultivation, ethanol or glycerol increased CYP2E1 activities, whereas 4-methylpyrazole or isoniazid inhibited. This different effect of the chemical inducers on CYP2E1 activi-ties persisted to subsequent 24 hr. Competitive inhibition study suggested that 4-methylpyrazole or isoniazid has stronger binding affinity to CYP2E1 than ethanol or glycerol. These results demonstrate that different binding affinity of the chemical inducers to the active site of CYP2E1 plays important role in determining real CYP2E1 activity in intact cells after treatment with the chemical inducers. Present study would be helpful in precise understanding of human CYP2E1-mediated toxicity.

• Journal of the Economic Geographical Society of Korea
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• v.14 no.4
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• pp.628-641
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• 2011

Focusing on the photonics industry as a new one and the photonics industry agglomeration in Gwangju, this study investigated the regional characteristics of the photonics industry's growth. To analyze the location and business network of photonics firms as foundation of industrial growth, in-depth interviews were conducted. It also aimed to review the meanings of regional policy to promote a strategic industry. As a result, it founded the differential growth of the photonics industry in the Capital region(with a market advantage) and Gwangju(with a policy advantage). Gwangju's regional policy had an importance that it formed a regional consensus and increased the momentum and variety to drive regional industrial development.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.69-69
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• 2003

Neuroprotective strategies have been appeared to be effective in a variety of stroke models. One of the major focuses has been related to the activities of estrogen. $17\beta$-estradiol valerate(EV) has been reported to exert neuroprotective effects when administered before an ischemic insult. The purpose of this study was to determine whether EV can protect against brain injury via estrogen receptor. Chronic and acute pretreatment can reduce the ischemic damage of focal cerebral ischemia in OVX rat, indicating that EV may be a new therapeutic class of drugs to prevent neuronal damage associated with cerebral ischemia. RNAs were extracted from the hippocampus of ovariectomized female rat with or without EV. Differential gene expression profiles were revealed(Bone morphogenetic protein type 1A receptor, Protein disulphide isomerase, cytochrome bc-1 complex core P, thiol-specific antioxidant protein). RT-PCR and in situ hybridization were used to validate the relative expression pattern obtained by the cDNA array. This Study was supported by the Korea Science and Engineering Foundation(KOSEF) through the Biohealth Products Research Center(BPRC), Inje University, Korea