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Effects of Some Amino Acids on Ammonia Secretion and Extracellular Protease Activity by Three Oomycetes in Synthetic Medium with or without Glucose

  • Ali, Esam H.
    • Mycobiology
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    • 제33권1호
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    • pp.23-29
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    • 2005
  • The effects of different concentrations of three amino acids as carbon and or nitrogen sources on mycelial dry weights, changes in pH values of synthetic medium, ammonia secretion and extracellular protease activity by three zoosporic fungi, pathogens of fish and shellfish, were studied. As compared with the control, the addition of isoleucine and aspartic acid as nitrogen sources were generally stimulative for mycelial dry weight production whereas phenylalanine was inhibitory irrespective to the tested fungal species. When amino acids served as carbon and nitrogen sources, the mycelial dry weights of the three fungi were increased (mostly non-significantly) relative to untreated control but weights were decreased as the concentrations of the three amino acids raised. The addition of individual amino acids as carbon and nitrogen sources to the medium significantly increased pH values of the medium comparable to the control. The addition of each of the three amino acids as carbon and nitrogen sources to the medium significantly induced ammonia secretion by the three species of zoosporic fungi. Ammonia secretion in synthetic medium amended with amino acids as nitrogen source raised by the three zoosporic fungi relative to untreated control except in case of Achlya racemosa treated with isoleucine. Extracellular protease activity was almost promoted in case of Achlya proliferoides and Saprolegnia furcata cultures treated with isoleucine and aspartic acid individually in presence of glucose and vice versa in case of phenylalanine. However, extracellular protease activity of A. racemosa decreased compared with the control at various concentrations of isoleucine and both phenylalanine and aspartic acid assumed inconsistent effects. Extracellular protease activity of the three zoosporic fungi in the medium devoid of glucose varied depending upon zoosporic fungal species, the tested amino acid and the applied concentrations. The values of protease activity were approximately less two folds than that obtained in presence of glucose.

하이브리드 수용모델을 이용한 서울시 PM2.5 오염원의 위치 추적 (Identification of Potential Source Locations of PM2.5 in Seoul using Hybrid-receptor Models)

  • 강병욱;강충민;이학성;선우영
    • 한국대기환경학회지
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    • 제24권6호
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    • pp.662-673
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    • 2008
  • Two hybrid receptor models, potential source contribution function (PSCF) and concentration weighted tracjectory (CWT), were compared for locating $PM_{2.5}$ sources contributing to the atmospheric $PM_{2.5}$ concentrations in Seoul. The source contribution estimates by chemical receptor model (CMB) receptor model were used to identify better source areas, Among the sources, soil, agricultural burning, marine aerosol, coal-fired power plant and Chinese aerosol were only considered for the study because these sources were more likely to be associated with the long-range transport of air pollutant. Both methods are based on combining chemical data with calculated air parcel backward trajectories. However, the PSCF analyses were performed with trajectories above the $75^{th}$ percentile criterion values, while the CWT analyses used all trajectories. This difference resulted in locating of different sources, which might be helpful to interpret locating of $PM_{2.5}$ sources, High possible source areas in source contribution of soil and agricultural burning contributing to the Seoul $PM_{2.5}$ were inland areas of Heibei and Shandong provinces (highest density areas of agricultural production and population) in China. The "Chinese aerosol" was used as a representative source for the $PM_{2.5}$ originated from urban area in China. High possible source areas for the aerosol were the cities in China where are relatively close to the receptor. This result suggests that Chinese aerosol is likely to be a useful tool in studies on source apportionment and identification in Korea.

A Search for Very Low-luminosity Objects in Gould Belt Clouds

  • Kim, Mi-Ryang;Lee, Chang Won;Dunham, Michael M.;Evans, Neal J II;Kim, Gwanjeong;Allen, Lori E
    • 천문학회보
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    • 제41권2호
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    • pp.38.3-39
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    • 2016
  • We present the results of a search for Very Low-Luminosity Objects (VeLLOs) in the Gould Belt (GB) clouds using infrared and sub-millimeter (sub-mm) data from 1.25 to $850{\mu}m$ and our N2H+ (J = 1-0) observations. We modified the criteria by Dunham et al. to select the VeLLOs in the GB clouds, finding 95 VeLLO candidates, 79 of which are newly identified in this study. Out of 95 sources, 44 were detected in both sub-mm continuum and N2H+ emission and were classified as Group A (the VeLLOs), and 51 sources detected in either sub-mm emission or N2H+ emission were classified with Group B as candidate VeLLOs. We find that these VeLLOs and the candidates are forming in environments different from those of the likely VeLLOs. Seventy-eight sources are embedded within their molecular clouds, and thus are likely VeLLOs forming in a dense environment. The remaining 17 sources are located in low-level extinction regions (Av < 1) connected to the clouds, and can be either background sources or candidate substellar objects forming in an isolated mode. The VeLLOs and the candidates are likely more luminous and their envelopes tend to be more massive in denser environments. The VeLLOs and the candidates are more populous in the clouds where more YSOs form, indicating that they form in a manner similar to that of normal YSOs. The bolometric luminosities and temperatures of the VeLLOs are compared to predictions of episodic accretion models, showing that the low luminosities for most VeLLOs can be well explained by their status in the quiescent phases of a cycle of episodic mass accretion.

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조사료원에 따른 한국 재래산양의 채식습성에 관한 연구 (A study on the food habits of Korean native goats fed with roughage sources)

  • 강병호;이인덕;이수기;이형석
    • 농업과학연구
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    • 제38권3호
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    • pp.445-452
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    • 2011
  • The object of this experiment was to investigate the food habits of Korean native goats fed with various roughage sources. The experimental trials were conducted at Unbong Animal Genetic Resources Station in 2008. The experimental roughages include five sources and 25 species in all; grasses and legumes: 5 species (mixed grasses, orchardgrass, tall fescue, alfalfa, white clover), native grasses and weeds: 5 species (mixed native grasses, Miscanthus sinensis Anderss, Arundinella hirta (Thunb.) Tanaka, Barnyard grass, short awn, forage crops and straw: 5 species (barley + hairy vetch, wheat + hairy vetch, rye silage, barley silage, baled rice straw), browse and fallen leaves: 5 species (Mixed browse, Oriental white oak, Quercus serrta Thunb., Oriental cherry fallen leaves, Japanese chestnut fallen leaves), and imported hay and straw: 5 species(timothy hay, tall fescue straw, annual ryegrass straw, klinegrass hay, alfalfa hay). Ten Korean native goats were selected which had nearly the same body weight (average $24{\pm}2.8$ kg). The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period (p<0.05). Among all the 25 species of roughages, the favorite intake species order by Korean native goat was observed like this: Quercus aliena, Querancas serrta Thunb and Mixed browse, which was a lower intake compared to other domestic herbivores. The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. On the other hand, compared to each roughage source, total intake amount by Korean native goats was showed as browse and fallen leaves (33.1%) among trials roughage sources. Based on these results, the food habit of Korean native goats seems to be closer to the browser.

조사료원에 따른 꽃사슴(Servus nippon)의 채식습성에 관한 연구 (A study on the food habits of Sika Deer (Saanen) fed with roughage sources)

  • 강병호;이인덕;이수기;이형석
    • 농업과학연구
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    • 제38권3호
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    • pp.437-444
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    • 2011
  • The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The experimental trials were conducted at Unbong Animal Genetic Resources Station in 2008. The experimental roughages include five sources and 25 species in all; grasses and legumes: 5 species (mixed grasses, orchardgrass, tall fescue, alfalfa, white clover), native grasses and weeds: 5 species (mixed native grasses, Miscanthus sinensis Anderss, Arundinella hirta (Thunb.) Tanaka, barnyard grass, short awn, forage crops (hay, silages and straw): 5 species (barley + hairy vetch, Wheat + hairy vetch, rye silage, barley silage, baled rice straw), browse and fallen leaves: 5 species (mixed browse, oriental white oak, Quercus serrta Thunb., oriental cherry fallen leaves, Japanese chestnut fallen leaves), and imported hays and straws: 5 species (timothy hay, tall fescue straw, annual ryegrass straw, klinegrass hay, alfalfa hay). Five sika deer were used as experimental animals and the averaged body weight was 95+5.4kg. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period(p<0.05). The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake roughage sources ranking by sika deer was observed like this: browse and fallen leaves (32.2%), grass and legumes (27.0%), native grasses and weeds (22.0%), imported hays (12.9%) and forages crops (5.5%) respectively. Although, the sika deer ate more browse leaves, but ate more roughage which had low fibrous contents (NDF and ADF), but high drymatter digestibility. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse and fallen leaves (32.2%). Based on the result, the food habit of sika deer seems to be closer to the typical browser.

Exploring the Effects of Carbon Sources on the Metabolic Capacity for Shikimic Acid Production in Escherichia coli Using In Silico Metabolic Predictions

  • Ahn, Jung-Oh;Lee, Hong-Weon;Saha, Rajib;Park, Myong-Soo;Jung, Joon-Ki;Lee, Dong-Yup
    • Journal of Microbiology and Biotechnology
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    • 제18권11호
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    • pp.1773-1784
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    • 2008
  • Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-scale in silico model of E. coli was conducted to quantify the theoretical maximum SA yield. The corresponding flux distributions fueled by different carbon sources under investigation were compared with respect to theoretical yield and energy utilization, thereby identifying the indispensable pathways for achieving optimal SA production on each carbon source. Subsequently, a shikimate-kinase-deficient E. coli mutant was developed by blocking the aromatic amino acid pathway, and the production of SA on various carbon sources was experimentally examined during 51 batch culture. As a result, the highest production rate, 1.92 mmol SA/h, was obtained when glucose was utilized as a carbon source, whereas the efficient SA production from glycerol was obtained with the highest yield, 0.21 mol SA formed per mol carbon atom of carbon source consumed. The current strain can be further improved to satisfy the theoretically achievable SA production that was predicted by in silico analysis.

MPC-based Two-stage Rolling Power Dispatch Approach for Wind-integrated Power System

  • Zhai, Junyi;Zhou, Ming;Dong, Shengxiao;Li, Gengyin;Ren, Jianwen
    • Journal of Electrical Engineering and Technology
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    • 제13권2호
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    • pp.648-658
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    • 2018
  • Regarding the fact that wind power forecast accuracy is gradually improved as time is approaching, this paper proposes a two-stage rolling dispatch approach based on model predictive control (MPC), which contains an intra-day rolling optimal scheme and a real-time rolling base point tracing scheme. The scheduled output of the intra-day rolling scheme is set as the reference output, and the real-time rolling scheme is based on MPC which includes the leading rolling optimization and lagging feedback correction strategy. On the basis of the latest measured thermal unit output feedback, the closed-loop optimization is formed to correct the power deviation timely, making the unit output smoother, thus reducing the costs of power adjustment and promoting wind power accommodation. We adopt chance constraint to describe forecasts uncertainty. Then for reflecting the increasing prediction precision as well as the power dispatcher's rising expected satisfaction degree with reliable system operation, we set the confidence level of reserve constraints at different timescales as the incremental vector. The expectation of up/down reserve shortage is proposed to assess the adequacy of the upward/downward reserve. The studies executed on the modified IEEE RTS system demonstrate the effectiveness of the proposed approach.

Novel Sources of Resistance to Phytophthora capsici on Pepper (Capsicum sp.) Landraces from Mexico

  • Retes-Manjarrez, Jesus Enrique;Rubio-Aragon, Walter Arturo;Marques-Zequera, Isidro;Cruz-Lachica, Isabel;Garcia-Estrada, Raymundo Saul;Sy, Ousmane
    • The Plant Pathology Journal
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    • 제36권6호
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    • pp.600-607
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    • 2020
  • Phytophthora capsici Leonian is a major pathogen of pepper worldwide and few resistance sources to this pathogen have been identified so far. The goals of this study were to identify new sources of resistance against P. capsici in Capsicum landraces and analyze the relationship between the resistance indicator of plant symptoms and some plant phenotype parameters of plant height, stem width, leaf length and leaf width. Thirty-two landraces of pepper were collected from fourteen states in Mexico. From each population, 36 plants were inoculated with 10,000 zoospores of P. capsici under controlled conditions. This experiment was repeated twice. Out of the 32 landraces, six showed high level of resistance, four showed intermediate resistance and five showed low level of resistance when compared with the susceptible control 'Bravo' and the resistant control 'CM334', indicating that these landraces are promising novel sources of resistance to P. capsici. There was no correlation between the symptoms and plant phenotype parameters. However, these parameters were not affected in the group classified as highly resistant, indicating that P. capsici does not affect the growing of these resistant pepper landraces. The other resistant groups were significantly affected in a differently manner regarding their phenotype, indicating that this pathogen reduce their growth in different ways. This study reports novel resistance sources with great potential that could be used in breeding programs to develop new pepper cultivars with durable resistance to P. capsici.

온라인 구매환경에서 제품 관여도와 연령 집단에 따른 지각된 품질이 구매 의도에 미치는 영향: 정보원천과 소셜미디어 플랫폼 조절효과 (Effect of Perceived Quality by Product Involvement and Age Group on Purchase Intention in Online Environments: A Moderating Role of Information Sources and Social Media Platform)

  • 이다솔;오민정
    • 산업경영시스템학회지
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    • 제44권3호
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    • pp.262-275
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    • 2021
  • The expansion of the online market is expected to change the purchasing environment. The purpose of this study is to examine the difference in the moderating effect of each characteristic on perceived quality and purchase intention according to the group according to product involvement and purchaser age. The first step is to identify the characteristics of online information sources and social media platforms through a literature review. Next, when perceived quality affects purchase intention, we verify the moderating effect according to the characteristics of online information sources and social media platforms. The moderating effect is verified at the stage by dividing it into a group according to product involvement and a group according to age. The following results were confirmed throughout the study: First, perceived quality significantly affects purchase intention. Second, in the relationship between perceived quality and purchase intention, the influence of the moderating effect is different depending on the high-involvement product and the low-involvement product. Third, it was confirmed that there was a difference in the moderating effect of online information sources and social media platforms in the relationship between perceived quality and purchase intention according to age. This study intends to increase consumers' purchase intentions by identifying specific age groups and product groups of involvement and establishing strategies suitable for the characteristics of online information sources and social media platforms.

The Comparative Immunomodulatory Effects of β-Glucans from Yeast, Bacteria, and Mushroom on the Function of Macrophages

  • Jang, Seon-A;Park, Sul-Kyoung;Lim, Jung-Dae;Kang, Se-Chan;Yang, Kwang-Hee;Pyo, Suh-Kneung;Sohn, Eun-Hwa
    • Preventive Nutrition and Food Science
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    • 제14권2호
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    • pp.102-108
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    • 2009
  • The comparative immunomodulatory effects of ${\beta}$-glucans isolated from mushroom fungi (Coriolus versicol), yeast (Saccharomyces cerevisiae) and bacteria (Agrobacterium) on the major functions of macrophages were evaluated. As parameters of macrophage functions, we examined tumoricidal activity, phagocytosis, nitric oxide (NO) production, and the induction of inducible NO synthetase (iNOS) in RAW264.7 cells, following treatments with ${\beta}$-glucans from the three different sources. The results indicated that all ${\beta}$-glucan treatments significantly induced tumoricidal activity in the RAW264.7 cells, with a remarkable effect shown by the beta-glucan from Agrobacterium at a concentration of $10{\mu}g/mL$. There was also a significant increase in iNOS-NO system activity in macrophages treated with ${\beta}$-glucans extracted from yeast; however, iNOS-NO system activity was not markedly changed by the treatment of ${\beta}$-glucans from C. versicolor mushroom fungi or Agrobacterium. Furthermore, the ${\beta}$-glucans from C. versicolor had a significant phagocytotic effect at concentrations of 1, 10, and $100{\mu}g/mL$. Taken together, the present data suggest that these ${\beta}$-glucans, isolated from three different sources, have different effects on macrophage function, and therefore, may have different clinical uses in different for various types of diseases.