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http://dx.doi.org/10.3746/jfn.2009.14.2.102

The Comparative Immunomodulatory Effects of β-Glucans from Yeast, Bacteria, and Mushroom on the Function of Macrophages  

Jang, Seon-A (Department of Herbal Medicine Resource, Kangwon National University, Institute of Bioscience and Biotechnology)
Park, Sul-Kyoung (Department of Herbal Medicine Resource, Kangwon National University, Institute of Bioscience and Biotechnology)
Lim, Jung-Dae (Department of Herbal Medicine Resource, Kangwon National University, Institute of Bioscience and Biotechnology)
Kang, Se-Chan (Department of Natural Medicine Resources, Semyung University)
Yang, Kwang-Hee (Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd)
Pyo, Suh-Kneung (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University)
Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University, Institute of Bioscience and Biotechnology)
Publication Information
Preventive Nutrition and Food Science / v.14, no.2, 2009 , pp. 102-108 More about this Journal
Abstract
The comparative immunomodulatory effects of ${\beta}$-glucans isolated from mushroom fungi (Coriolus versicol), yeast (Saccharomyces cerevisiae) and bacteria (Agrobacterium) on the major functions of macrophages were evaluated. As parameters of macrophage functions, we examined tumoricidal activity, phagocytosis, nitric oxide (NO) production, and the induction of inducible NO synthetase (iNOS) in RAW264.7 cells, following treatments with ${\beta}$-glucans from the three different sources. The results indicated that all ${\beta}$-glucan treatments significantly induced tumoricidal activity in the RAW264.7 cells, with a remarkable effect shown by the beta-glucan from Agrobacterium at a concentration of $10{\mu}g/mL$. There was also a significant increase in iNOS-NO system activity in macrophages treated with ${\beta}$-glucans extracted from yeast; however, iNOS-NO system activity was not markedly changed by the treatment of ${\beta}$-glucans from C. versicolor mushroom fungi or Agrobacterium. Furthermore, the ${\beta}$-glucans from C. versicolor had a significant phagocytotic effect at concentrations of 1, 10, and $100{\mu}g/mL$. Taken together, the present data suggest that these ${\beta}$-glucans, isolated from three different sources, have different effects on macrophage function, and therefore, may have different clinical uses in different for various types of diseases.
Keywords
${\beta}$-glucan; Corious versicolor; Saccharomyces cerevisiae; Agrobacterium; macrophages;
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1 Muller A, Rice PJ, Ensley HE, Coogan PS, Kalbfleisch JH, Kelley JL, Love EJ, Portera CA, Ha T, Browder IW, Williams DL. 1996. Receptor binding and internalization of water-soluble (1$\rightarrow$3)-beta-D-glucan biologic response modifier in two monocyte/macrophage cell lines. J Immunol 156: 3418-3425
2 Williams DL, Mueller A, Browder W. 1996. Glucan-based macrophage stimulators: a review of their anti-infective potential. Clin Immunother 5: 392-399   DOI
3 Yadomae T. 2000. Structure and biological activities of fungal $\beta$-1,3-glucans. Yakugaku Zasshi 120: 413-431   DOI
4 Imura H, Ohno N, Suzuki I, Yadomae T. 1985. Purification, antitumor activity, and structural characterization of $\beta$-1,3-glucan from Peziza vesiculosa. Chem Pharm Bull 33: 5096-5099   DOI   ScienceOn
5 Hahn H, Kaufmann SH. 1981. The role of cell-mediated immunity in bacterial infections. Rev Infect Dis 3: 1221-1250   DOI
6 Verstovsek S, Maccubbin D, Mihich E. 1992. Tumoricidal activation of murine resident peritoneal macrohages by interleukin 2 and tumor necrosis factor. Cancer Res 52: 3880-3885
7 Adams DO, Hamilton TA. 1984. The cell biology of macrophage activation. Annu Rev Immunol 2: 283-318   DOI   ScienceOn
8 Fidler IJ, Kleinerman ES. 1983. Therapy of cancer metastasis by systemic activation of macrophages: from the bench to the clinic. Res Immuno 144: 284-287   DOI   ScienceOn
9 Keller R, Keist R. 1989. Abilities of activated macrophages to manifest tumoricidal activity and to generate reactive nitrogen intermediates: a comparative study in vitro and ex vivo. Biochem Biophys Res Commun 164: 968-973   DOI   ScienceOn
10 Nathan CF. 1987. Secretory products of macrophages. J Clin Invest 79: 319-326   DOI   ScienceOn
11 Williams DL, Pretus HA, Browder IW. 1992. Application of aqueous gel permeation chromatography with in-line multi-angle laser light scattering and differential viscometry detectors for the characterization of natural product carbohydrate pharmaceuticals. J Liq Chromatogr 15: 2297-2309   DOI
12 Ding AH, Nathan CF, Stuehr DJ. 1988. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol 141: 2407-2412
13 Chen J, Seviour R. 2007. Medicinal importance of fungal beta-(1$\rightarrow$3), (1$\rightarrow$6)-glucans. Mycol Res 111: 635-652   DOI   ScienceOn
14 Kuo YC, Huang YL, Chen CC, Lin YS, Chuang KN, Tsai WJ. 2002. Cell cycle progression and cytokine gene expression of human peripheral blood mononuclear cells modulated by Agaricus blazei. J Lab Clin Med 140: 176-187   DOI   ScienceOn
15 Taylor PR, Brown GD, Reid DM, Willment JA, Martinez-Pomares L, Gordon S. 2002. The $\beta$-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J Immunol 169: 3876-3882   DOI
16 Bredt DS, Snyder SH. 1994. Nitric oxide: a physiologic messenger molecule. Annu Rev Biochem 63: 175-195   DOI   ScienceOn
17 Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63   DOI   ScienceOn
18 Okimura T, Ogawa M, Yamauchi T. 1986. Stress and immune responses. III. Effect of resistant stress on delayed type hypersensitivity (DTH) response, natural killer (NK) activity and phagocytosis in mice. Jpn J Pharmacol 41: 229-235   DOI
19 Shao BM, Xu W, Dai H, Tu P, Li Z, Gao XM. 2004. A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochem Biophys Res Commun 320: 1103-1111   DOI   ScienceOn
20 Lee KY, You HJ, Jeong HG, Kang JS, Kim HM, Rhee SD, Jeon YJ. 2004. Polysaccharide isolated from Poria cocos sclerotium induces NF-$\kappa$B/Rel activation and iNOS expression through the activation of p38 kinase in murine macrophages. Int Immunopharmacol 4: 1029-1038   DOI   ScienceOn
21 Rice PJ, Kelley JL, Kogan G, Ensley HE, Kalbfleisch JH, Browder IW, Williams DL. 2002. Human monocyte scavenger receptors are pattern recognition receptors for (1$\rightarrow$3)-beta-D-glucans. J Leukoc Biol 72: 140-146
22 Herre J, Marshall AS, Caron E, Edwards AD, Williams DL, Schweighoffer E, Tybulewicz, V, Reise Sousa C, Gordon S, Brown GD. 2004. Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages. Blood 104: 4038-4045   DOI   ScienceOn
23 Vereschagin EI, van Lambalgen AA, Dushkin MI, Schwartz YS, Polyakov L, Heemskerk A, Huisman E, Thijs LG, van den Bos GC. 1998. Soluble glucan protects against endotoxin shock in the rat: the role of the scavenger receptor. Shock 9: 193-198   DOI   ScienceOn
24 Hsu HY, Hua KF, Lin CC, Lin CH, Hsu J, Wong CH. 2004. Extract of reishi polysaccharides induces cytokine expression via TLR4-modulated protein kinase signaling pathways. J Immunol 173: 5989-5999   DOI
25 Di Luzio NR, Williams DL, McNamee RB, Edwards BF, Kitahama A. 1979. Comparative tumor-inhibitory and anti- bacterial activity of soluble and particulate glucan. Int J Cancer 24: 773-779   DOI
26 Burleson GR, Dean JH, Munson AE. 1995. Introduction to the mononuclear phagocyte system. In Methods in immunotoxicology. Wiley-Liss, New York. Vol 2, p 3-13
27 Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E. 2007. Effects of beta-glucans on the immune system. Medicina (Kaunas) 43: 597-606