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The Comparative Immunomodulatory Effects of β-Glucans from Yeast, Bacteria, and Mushroom on the Function of Macrophages

  • Jang, Seon-A;Park, Sul-Kyoung;Lim, Jung-Dae;Kang, Se-Chan;Yang, Kwang-Hee;Pyo, Suh-Kneung;Sohn, Eun-Hwa
    • Preventive Nutrition and Food Science
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    • v.14 no.2
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    • pp.102-108
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    • 2009
  • The comparative immunomodulatory effects of ${\beta}$-glucans isolated from mushroom fungi (Coriolus versicol), yeast (Saccharomyces cerevisiae) and bacteria (Agrobacterium) on the major functions of macrophages were evaluated. As parameters of macrophage functions, we examined tumoricidal activity, phagocytosis, nitric oxide (NO) production, and the induction of inducible NO synthetase (iNOS) in RAW264.7 cells, following treatments with ${\beta}$-glucans from the three different sources. The results indicated that all ${\beta}$-glucan treatments significantly induced tumoricidal activity in the RAW264.7 cells, with a remarkable effect shown by the beta-glucan from Agrobacterium at a concentration of $10{\mu}g/mL$. There was also a significant increase in iNOS-NO system activity in macrophages treated with ${\beta}$-glucans extracted from yeast; however, iNOS-NO system activity was not markedly changed by the treatment of ${\beta}$-glucans from C. versicolor mushroom fungi or Agrobacterium. Furthermore, the ${\beta}$-glucans from C. versicolor had a significant phagocytotic effect at concentrations of 1, 10, and $100{\mu}g/mL$. Taken together, the present data suggest that these ${\beta}$-glucans, isolated from three different sources, have different effects on macrophage function, and therefore, may have different clinical uses in different for various types of diseases.

Effects of Different Culture Conditions on In Vitro Production of Bovine Embryos (체외배양 조건이 소 체외수정란의 생산에 미치는 효과)

  • 조성근;노규진;이정규;이효종;최상용
    • Journal of Embryo Transfer
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    • v.15 no.3
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    • pp.271-277
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    • 2000
  • This study was conducted to establish the optimal culture conditions for in vitro production of bovine embryos derived from slaughter house ovaries. Cumulus-oocyte- complexes (COCs) collected by aspiration from follicles of 2~7 mm in diameter were matured in Ham's F-10 medium supplemented with 0.01 $\mu\textrm{g}$/m1 epidermal growth factor (EGF) at 39$^{\circ}C$ in a humidified atmosphere of 5% $CO_2$in air. After 24 hrs of culture, the oocytes were co-cultured with epididymal sperm selected off by Percoll-density gradient in TALP medium for 24 hrs. The presumptive zygotes were cultured in HECM-6 medium for 3 d post-insemination, and followed by cultured in TCM199 medium until 7 to 10d post-insemination. The cultures were compared of their cleavage and development into later stage in culture medium by additions of different protein sources (PVA, BSA and BCS) and by different embryo density. The rates of cleavage and development rates into blastocyst were not significantly (P<0.05) different among the culture media containing with BSA (75.0% and 40.5%), BCS (76.7% and 38.0%) and PVA (72.5% and 42.2%), respectively. Significantly (P<0.05) higher blastocysts rates were obtained in culturing of 30 and 40 embryos in each 50$\mu$l droplets of culture medium than in 5, 10 and 20 embryos. These results indicate that the optimal density of embryos is 30~40 embryos in a 50$\mu$l droplet of culture medium. Furthermore there is no effect of different protein sources on early embryonic development.

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Effect of Feeding with Different Source of Carbohydrate and HCA on Body Weight Gain and Lipid Metabolism in Rats (탄수화물 급원에 따른 HCA의 공급이 흰쥐의 체중 및 지질 대사에 미치는 영향)

  • Son, Young-Ae;Jeong, Hye-Jin;Shim, Jee-Ae;Kwon, Sang-Hee;Kim, Mi-Kyung
    • Journal of Nutrition and Health
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    • v.40 no.8
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    • pp.675-683
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    • 2007
  • This study was conducted to investigate the effects of feeding different carbohydrate sources and garcinia cambogia extract(HCA) on body weight and lipid metabolism. Fifty 10-month-old male Sprague-Dawley rats weighting $635{\pm}6g$ were randomly divided into 5 groups and fed different experimental diets for 4 weeks. The carbohydrate(CHO) sources of each group were cornstarch(control group, 100% of CHO), fructose(F group and FH group, 25% of CHO) and sucrose(S group and SH group, 25% of CHO). FH group and SH group were fed diets containing 1%(W/W) of HCA. Food intake, body weight gain, and calorie efficiency were not significantly different among the groups. Perirenal fat pad weight of FH group was significantly lower than F group, but epididymal fat pad weight was not different among the groups. Fasting glucose level were not significant among the groups. Plasma lipid profile of FH or SH group was slightly lower than F or S group, respectively. The degree of difference of plasma lipid level was greater between F and FH group than those of between S and SH group. In liver, total lipid, triglyceride and total cholesterol level were slightly higher in F group than S group, and tended to be lower in FH group than F group, but tended to be higher in SH group than S group. Liver citrate lyase activity were not significant among the groups. These results suggest that HCA is potential material for reduction of body weight and improvement of plasma lipid profiles. But, there was no difference between fructose intake with HCA and sucrose intake with HCA in reduction of body weight and lipid metabolism.

Physical and numerical modelling of the inherent variability of shear strength in soil mechanics

  • Chenari, Reza Jamshidi;Fatahi, Behzad;Ghoreishi, Malahat;Taleb, Ali
    • Geomechanics and Engineering
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    • v.17 no.1
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    • pp.31-45
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    • 2019
  • In this study the spatial variability of soils is substantiated physically and numerically by using random field theory. Heterogeneous samples are fabricated by combining nine homogeneous soil clusters that are assumed to be elements of an adopted random field. Homogeneous soils are prepared by mixing different percentages of kaolin and bentonite at water contents equivalent to their respective liquid limits. Comprehensive characteristic laboratory tests were carried out before embarking on direct shear experiments to deduce the basic correlations and properties of nine homogeneous soil clusters that serve to reconstitute the heterogeneous samples. The tests consist of Atterberg limits, and Oedometric and unconfined compression tests. The undrained shear strength of nine soil clusters were measured by the unconfined compression test data, and then correlations were made between the water content and the strength and stiffness of soil samples with different consistency limits. The direct shear strength of heterogeneous samples of different stochastic properties was then evaluated by physical and numerical modelling using FISH code programming in finite difference software of $FLAC^{3D}$. The results of the experimental and stochastic numerical analyses were then compared. The deviation of numerical simulations from direct shear load-displacement profiles taken from different sources were discussed, potential sources of error was introduced and elaborated. This study was primarily to explain the mathematical and physical procedures of sample preparation in stochastic soil mechanics. It can be extended to different problems and applications in geotechnical engineering discipline to take in to account the variability of strength and deformation parameters.

Fabrication, characterization, simulation and experimental studies of the ordinary concrete reinforced with micro and nano lead oxide particles against gamma radiation

  • Mokhtari, K.;Kheradmand Saadi, M.;Ahmadpanahi, H.;Jahanfarnia, Gh.
    • Nuclear Engineering and Technology
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    • v.53 no.9
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    • pp.3051-3057
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    • 2021
  • The concrete is considered as an important radiation shielding material employed widely in nuclear reactors, particle accelerators, laboratory hot cells and other different radiation sources. The present research is dedicated to the shielding properties study of the ordinary concrete reinforced with different weight fractions of lead oxide micro/nano particles. Lead oxide particles were fabricated by chemical synthesis method and their properties including the average size, morphological structure, functional groups and thermal properties were characterized by XRD, FESEM-EDS, FTIR and TGA analysis. The gamma ray mass attenuation coefficient of concrete composites has been calculated and measured by means of the Monte Carlo simulation and experimental methods. The simulation process was based on the use of MCNP Monte Carlo code where the mass attenuation coefficient (μ/ρ) has been calculated as a function of different particle sizes and filler weight fractions. The simulation results showed that the employment of the lead oxide filler particles enhances the mass attenuation coefficient of the ordinary concrete, drastically. On the other hand, there are approximately no differences between micro and nano sized particles. The mass attenuation coefficient was increased by increasing the weight fraction of nanoparticles. However, a semi-saturation effect was observed at concentrations more than 10 wt%. The experimental process was based on the fabrication of concrete slabs filled by different weight fractions of nano lead oxide particles. The mass attenuation coefficients of these slabs were determined at different gamma ray energies using 22Na, 137Cs and 60Co sources and NaI (Tl) scintillation detector. The experimental results showed that the HVL parameter of the ordinary concrete reinforced with 5 wt% of nano PbO particles was reduced by 64% at 511 keV and 48% at 1332 keV. Reasonable agreement was obtained between simulation and experimental results and showed that the employment of nano PbO particles is more efficient at low gamma energies up to 1Mev. The proposed concrete is less toxic and could be prepared in block form instead of toxic lead blocks.

Physicochemical Properties of Starch Affected by Molecular Composition and Structures: A Review

  • Srichuwong, Sathaporn;Jane, Jay-Iin
    • Food Science and Biotechnology
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    • v.16 no.5
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    • pp.663-674
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    • 2007
  • Starches from different botanical sources differ in the ratio of amylose to amylopectin contents, molecular structures of amylose and amylopectin, granule morphology, and minor-component contents. These structural features result in different gelatinization, pasting, retrogradation properties, and enzyme digestibility of starch granules. In this review, compositions and molecular structures of starches and their effects on the physicochemical properties are summarized and discussed.

LASER ARC HYBRID WELDING

  • Dilthey, Ulrich;Keller, Hanno
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.162-168
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    • 2002
  • Hybrid Welding means the coupling of the energy of two different energy sources in a common process zone. This paper describes prospects in laser-arc-hybrid-welding. Different kinds of lasers ($CO_2$ laser and Nd:YAG laser) and arc processes (TIG, Plasma and GMA) are considered.

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Characterization of Polyacetylene Contents in Wild Mountain Ginseng and Cultured Ginseng (산삼 및 재배인삼의 폴리아세틸렌 함량 특성)

  • 장문식;유병삼;변상요
    • KSBB Journal
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    • v.18 no.6
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    • pp.440-442
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    • 2003
  • Panaxynol and panaxydol are major polyacetylene compounds in Ginseng and Wild Mountain Ginseng. Their contents in Korean wild mountain ginseng, Chinese mountain ginseng, and cultured ginseng were analysed by GC with FID. The content ratio of panaxynol to panaxydol was clearly different in various ginsengs. They were 1.81, 0.87, and 0.42 for Korean wild mountain ginseng, Chinese wild mountain ginseng, and cultured ginseng, respectively. The ratio difference could be used as a marker to identify various ginseng from different sources.

Various kinds of data and reliability assessment (여러 가지 데이터와 신뢰성 평가)

  • Baik, Jai-Wook
    • Journal of Applied Reliability
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    • v.9 no.4
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    • pp.303-317
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    • 2009
  • There are a lot of different data in a company. Some of the data can be modified to produce valuable information on reliability. In this study different types of data that can be obtained in a company are reviewed. Reliability related data that can be taken throughout the life cycle of a product are also reviewed. Developing a method of gathering all of the pertinent data from the various sources and databases and pulling them into one central location is explained.

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High resolution 3D display using time-multiplexed overlapped projection

  • Baasantseren, Ganbat;Park, Jae-Hyeung;Kim, Nam
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.1338-1340
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    • 2009
  • High resolution three-dimensional integral imaging display is proposed. Each time-multiplexed image is projected with different incident angle on same array of elemental lenses. Those images are collected at different positions in focal plane of lens array, and thus the number of the point light sources increases and their spacing decreases. Therefore, proposed method can create high resolution 3D images.

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