Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effect of Feeding with Different Source of Carbohydrate and HCA on Body Weight Gain and Lipid Metabolism in Rats

탄수화물 급원에 따른 HCA의 공급이 흰쥐의 체중 및 지질 대사에 미치는 영향

  • Son, Young-Ae (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Jeong, Hye-Jin (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Shim, Jee-Ae (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kwon, Sang-Hee (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Mi-Kyung (Department of Nutritional Science and Food Management, Ewha Womans University)
  • 손영애 (이화여자대학교 식품영양학과) ;
  • 정혜진 (이화여자대학교 식품영양학과) ;
  • 심지애 (이화여자대학교 식품영양학과) ;
  • 권상희 (이화여자대학교 식품영양학과) ;
  • 김미경 (이화여자대학교 식품영양학과)
  • Published : 2007.12.30
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Abstract

This study was conducted to investigate the effects of feeding different carbohydrate sources and garcinia cambogia extract(HCA) on body weight and lipid metabolism. Fifty 10-month-old male Sprague-Dawley rats weighting $635{\pm}6g$ were randomly divided into 5 groups and fed different experimental diets for 4 weeks. The carbohydrate(CHO) sources of each group were cornstarch(control group, 100% of CHO), fructose(F group and FH group, 25% of CHO) and sucrose(S group and SH group, 25% of CHO). FH group and SH group were fed diets containing 1%(W/W) of HCA. Food intake, body weight gain, and calorie efficiency were not significantly different among the groups. Perirenal fat pad weight of FH group was significantly lower than F group, but epididymal fat pad weight was not different among the groups. Fasting glucose level were not significant among the groups. Plasma lipid profile of FH or SH group was slightly lower than F or S group, respectively. The degree of difference of plasma lipid level was greater between F and FH group than those of between S and SH group. In liver, total lipid, triglyceride and total cholesterol level were slightly higher in F group than S group, and tended to be lower in FH group than F group, but tended to be higher in SH group than S group. Liver citrate lyase activity were not significant among the groups. These results suggest that HCA is potential material for reduction of body weight and improvement of plasma lipid profiles. But, there was no difference between fructose intake with HCA and sucrose intake with HCA in reduction of body weight and lipid metabolism.

Keywords

References

  1. Bray GA. Definition, measurement and classification of the syndromes of obesity. Int J Obes 1978; 2 (2) : 99-112
  2. WHO. Fact sheet No 311. Obesity and overweight; 2006
  3. Ministry of Health and Welfare. 2001 National health and nutrition survey, overview; 2002
  4. Ministry of Health and Welfare. 2005 National health and nutrition survey, overview; 2006
  5. Kim YH, Yoo JY, Lee EG, Kim KB, Jo DH, Hwang JY. Effect of a dietary supplement consisting of Phaseolus vulgaris and Garcinia cambogia (HCA) on the lipid level and body weight. J Korean Soc Food Sci Nutr 2004; 33 (3): 518-522 https://doi.org/10.3746/jkfn.2004.33.3.518
  6. Elliott SS, Keirn NL, Stem JS, Teff K, Havel PJ. Fructose, weight gain, and the insulin resistance syndrome. Am J Clin Nutr 2002; 76 (5) : 911-922 https://doi.org/10.1093/ajcn/76.5.911
  7. Gaby AR. Adverse effects of dietary fructose. Altern Med Rev 2005; 10 (4): 294-306
  8. Reaven GM, Risser TR, Chen Y-Dl, Reaven EP. Characterization of a model of dietary induced hypertriglyceridemia in young, nonobese rats. J Lip Res 1979; 20: 371-378
  9. Lombardo YB, Chicco A, Mocchiutti N, Rodi M, Nusimovich B, Gutman R. Effects of sucrose diet on insulin secretion in vivo and in vitro, and on triglyceride storage and mobilisation of hearts of rats. Horm Metab Res 1983; 15: 69-76 https://doi.org/10.1055/s-2007-1018633
  10. Mancini M, Mattock M, Rabaya E, Chait A, Lewis B. Studies of the mechanism of carbohydrate-induced lipemia in normal man. Atherosclerosis 1973; 17: 445-454 https://doi.org/10.1016/0021-9150(73)90034-8
  11. Uusitupa MI. Fructose in diabetic diet. Am J Clin Nutr 1994; 59 (3): 753S-757S
  12. Bantle JP, Laine DC, Thomas JW. Metabolic effects of dietary fructose and sucrose in types I and II diabetic subjects. JAMA 1986; 256(23): 3241-3246 https://doi.org/10.1001/jama.256.23.3241
  13. Uphof JC. Garcinia Cambogia derouss. Dictionary of Economic Plants, second edition. New York: Verlag von J Cramer; 1968. p.237
  14. Lily MP, Judith M. Medicinal plants of East and Southeast Asia. Cambridge, Mass; The MIT Press; 1980. p.174-176
  15. Ishihara K, Oyaizu S, Onuki K, Lim K, Fushiki T. Chronic (-) hydroxy citrate administration spares carbohydrate utilization and promotes lipid oxidation during exercise in mice. J Nutr 2000; 130: 2990-2995 https://doi.org/10.1093/jn/130.12.2990
  16. Leonhardt M, Hrupka B, Langhans W. Effect of hydroxycitrate on food intake and body weight regain after a period of restrictive feeding in male rats. Physiol Behav 2001; 74 (1-2): 191-196 https://doi.org/10.1016/S0031-9384(01)00509-1
  17. Leonhardt M, Langhans W. Hydroxycitrate has long-term effects on feeding behavior, body weight regain and metabolism after body weight loss in male rats. J Nutr 2002; 132: 1977-1982 https://doi.org/10.1093/jn/132.7.1977
  18. Sullivan AC, Triscari J, Hamilton JG, Miller ON. Effect of (-)hydroxy citrate upon the accumulation of lipid in the rat. II. Appetite. Lipids 1974; 9: 129-134 https://doi.org/10.1007/BF02532137
  19. Rao RN, Sakariah KK. Lipid-lowering and antiobesity effect of (-) hydroxy citric acid. Nutr Res 1988; 8: 209-212 https://doi.org/10.1016/S0271-5317(88)80024-1
  20. Hayamizu K, Ishii Y, Kaneko I, Shen MZ, Okuhara Y, Shigematsu N. Effects of garcinia cambogia (hydroxycitric acid) on visceral fat accumulation: a double-blind, randomized, placebocontrolled trial. Curr Ther Res Clin Exp 2003; 64: 551-567 https://doi.org/10.1016/j.curtheres.2003.08.006
  21. Preuss HG, Bagchi D, Bagchi M, Rao CVS, Satyanarayana S, Dey DK. Efficacy of a novel, natural extract of (-) -hydroxycitric acid (HCA-SX) and a combination ofHCA-SX, niacin-bound chromium and gymnema sylvestre extract in weight management in human volunteers: a pilot study. Nutr Res 2004; 24: 45-58 https://doi.org/10.1016/j.nutres.2003.09.007
  22. Jena BS, Jayaprakasha GK, Singh RP, Sakariah KK. Chemistry and biochemistry of (-) -hydroxycitric acid from garcinia. J Agric Food Chem 2002; 50: 10-22 https://doi.org/10.1021/jf010753k
  23. Basciano H, Federico L, Adeli K. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr & Meta 2005; 2 (5): 1-24 https://doi.org/10.1186/1743-7075-2-1
  24. Vray GA, Nielsen SJ, Popkin BM. Consumption of high-fructose com syrup in beverages may playa role in the epidemic of obesity. Am J Clin Nutr 2004; 79 (4) : 537-543 https://doi.org/10.1093/ajcn/79.4.537
  25. Petersen KF, Laurent D, Yu C, Cline GW, Shulman GI. Stimulating effects of low-dose fructose on insulin-stimulated hepatic glycogen synthesis in humans. Diabetes 2001; 50: 1263-1268 https://doi.org/10.2337/diabetes.50.6.1263
  26. Park JY, Kim MK. Effect of feeding garcinia cambgia extract (HCA) and/or L-camitine and exercise on body weight in rats. Korean J Nutr 2005; 38(8): 637-648
  27. Burdock G, Bagchi M, Bagchi D. Garcinia cambogia toxicity is misleading. Food and chemical toxicology 2005; 43 (11): 1683-1684 https://doi.org/10.1016/j.fct.2005.05.011
  28. Saito M, Veno M, Ogino S, Kubo K, Nagata J, Takeuchi M. High dose of garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis. Food Chem Taxical 2005; 43 (3): 411-419 https://doi.org/10.1016/j.fct.2004.11.008
  29. Frings CS, Dunn RT. A colorimetric method for determination of total serum lipid based on the sulfuric-phospho-vanillin reaction. Am J Cli Nutr 1970; 53 (1): 89-91
  30. Bligh EG, Dyer WI. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37: 911-917 https://doi.org/10.1139/y59-099
  31. Melnick JZ, Srere PA, Elshourbagy NA, Moe OW, Preisig PA, Alpern RJ. Adenosine triphosphate citrate lyase mediates hypocitraturia in rats. J Clin Invest 1996; 98 (10) : 2381-2387 https://doi.org/10.1172/JCI119051
  32. Melnick JZ, Preisig PA, Alpern RJ, Baum M. Renal citrate metabolism and urinary citrate excretion in the infant rat. Kidney Int 2000; 57(3): 891-897 https://doi.org/10.1046/j.1523-1755.2000.057003891.x
  33. Srere PA. The citrate cleavage enzyme. J Biol Chem 1959; 234: 2544-2547
  34. Cottam GL, Srere PA. The sulfhydryl groups of citrate cleavage enzyme. Arch Biochem Biophys 1969; 130 (1) : 304-311 https://doi.org/10.1016/0003-9861(69)90037-X
  35. Kovacs EMR, Westerterp-Plantenga MS, Vries ND, Brous F, Saris WHM. Effect of 2-week ingestion of (-) -hydroxycitrate and (-) -hydroxycitrate combined with middle-chain triglycerides on satiety and food intake. Phys Behavior 2001; 74: 543-549 https://doi.org/10.1016/S0031-9384(01)00594-7
  36. Mattes RD, Bormann L. Effect of (-) -hydroxycitric acid on appetite variables. Phys Behavior 2000; 71: 87-94 https://doi.org/10.1016/S0031-9384(00)00321-8
  37. Kim SB, Park HR, Moon SJ, Kim JH, An KM, Yeo IH. Influence of (-) -hydroxycitrate on food intake, body weight and lipogenesis in rats. Korean J Nutr 1997; 30 (2): 123-131
  38. Leonhardt M, Langhans W. Hydroxycitrat has long-term effects on feeding behavior, body weight regain and metabolism after body weight loss in male rats. J Nutr 2002; 132: 1977-1982 https://doi.org/10.1093/jn/132.7.1977
  39. Stre1ing GH, NcCafferty MR, O'Neill JJ. ${\beta}-Hydroxybutyrate$ as a precursor to the acetyl moiety of acetylcholine. J Neurochem 1981; 37: 1250-1259 https://doi.org/10.1111/j.1471-4159.1981.tb04675.x
  40. Roy S, Rink C, Khanna S, Phillips C, Bagchi D, Bagchi M, Sen CK. Body weight and abdominal fat gene expression profile in response to a novel hydroxycitric acid-based dietary supplement. Gene Expr 2004;11 (5-6) :251-262 https://doi.org/10.3727/000000003783992289
  41. Kwon TD, Kim KH, Kim JY, Yeo YH, Lim KW. The effects of hydroxy citric acid supplemetation and swimming on obesity and lipid metabolism in high-fat diet fed rats. Korean Journal of Exercise Nutrition 2003; 7 (1) : 87-92
  42. Grant AM, Christie MR, Ashcroft SJ. Insulin release from human pancreatic islets in vitro. Diabetologia 1980; 19: 114-117 https://doi.org/10.1007/BF00421856
  43. Curry DL. Effects of mannose and fructose on the synthesis and secretion of insulin. Pancreas 1989; 4: 2-9 https://doi.org/10.1097/00006676-198902000-00002
  44. Kasim-Karakas SE, Vriend H, A1mario R, Chow LC, Goodman MN. Effects of dietary carbohydrates on glucose and lipid metabolism in golden Syrian hamsters. J Lab Clin Med 1996; 128: 208-213 https://doi.org/10.1016/S0022-2143(96)90013-X
  45. Blakely SR, Hallfrisch J, Reiser S, Prather ES. Long-term effects of moderate fructose feeding on glucose tolerance parameters in rats. J Nutr 1981; 111: 307-314 https://doi.org/10.1093/jn/111.2.307
  46. Kim HK, Kim DK, Rye SP, Lim KW, Lee Sc. Effects of (-)hydroxycitrate acid ingestion on glycogen loading in rats. The Korean Journal of Physical Education 2003; 42 (5): 755-761
  47. Sullivan C, Triscari J. Metabolic regulation as a control for lipid disorders. 1. Influence of (-) -hydroxycitrate in experimentally induces obesity in the rodent. Am J Clin Nutr 1977; 30 (5) : 767-776 https://doi.org/10.1093/ajcn/30.5.767
  48. Bantle JP, Swanson JE, Thomas W, Laine DC. Metabolic effects of dietary fructose in diabetic subjects. Diabetes Care 1992; 15: 1468-1476 https://doi.org/10.2337/diacare.15.11.1468
  49. Brandt K, Langhans W, Geary N, Leonhardt M. Beneficial and deleterious effects of hydroxycitrate in rats fed a high-fructose diet. Nutrition 2006; 22 (9): 905-912 https://doi.org/10.1016/j.nut.2006.05.015
  50. van Loon LJ, van Rooijen JJ, Niesen B, Verhagen H, Saris WH, Wagenmakers AJ. Effects of acute (-) -hydroxycitrate supplementation on substrate metabolism at rest and during exercise in human. Am JClin Nutr 2000; 72: 1445-1450 https://doi.org/10.1093/ajcn/72.6.1445
  51. Bantle JP, Raatz SK, Thomas W, georgopoulos A. Effects of dietary fructose on plasma lipids in healthy subjects. Am J Clin Nutr 2000; 72: 1128-1134 https://doi.org/10.1093/ajcn/72.5.1128
  52. Reiser S, Powell AS, Scholfield DJ, Panda P, Fields M, Canary JJ. Day-long glucose, insulin and fructose responses of hyperin- sulinemic and nonhyperinsulinemic men adapted to diets containing either fructose or high-amylose corn starch. Am J Clin Nutr 1989; 50: 1008-1014 https://doi.org/10.1093/ajcn/50.5.1008
  53. Frayn KN, Kingman SM. Dietary sugars and lipid metabolism in humans. Am J Clin Nutr 1995; 62: 250S-261S
  54. Brunzell JD, Hazzard WR, Porte DJ, Bierman EL. Evidence for a common, saturable, triglyceride removal mechanism for chylomicrons and very low density lipoproteins in man. J Clin Invest 1973; 52: 1578-1585 https://doi.org/10.1172/JCI107334
  55. Albrink MJ, Ullrich IH. Interaction of dietary sucrose and fiber on serum lipids in healthy young men fed high carbohydrate diets. Am J Clin Nutr 1986; 43: 419-428 https://doi.org/10.1093/ajcn/43.3.419
  56. Klimes I, Sebokova E, Vrana A, Kazdova L. Raised dietary intake of n-3 polyunsaturated fatty acids in high sucrose-induced insulin resistance. Animals studies. Ann N Y Acad Sci 1993; 14(683): 69-81 https://doi.org/10.1111/j.1749-6632.1901.tb55051.x
  57. Gutman R, Basilico MZ, Mocchiutti N, Chicco A, Lombardo YB. Diazoxide prevents the development of hormonal and metabolic abnormalities present in rats fed a sucrose-rich diet. Harm Metab Res 1985; 17: 491-494 https://doi.org/10.1055/s-2007-1013587
  58. Gutman R, Basilico MZ, Chicco A, Rodi MA, Nusimovich B, Lombardo VB. Effects of tiadenol and clofibrate on plasma post heparin lipolytic hepatic, extrahepatic and monoglyceride hydrolase activities in rats with hypertriglyceridemia induced by a sucrose rich diet. Horm Metab Res 1985; 17: 169-175 https://doi.org/10.1055/s-2007-1013484
  59. Belahsen R, Deshaies Y. Involvement of insulinemia in the postprandial hypotriacylglycerolemia induced by prazosin in the rat. Metabolism 1993; 42(10): 1301-1309 https://doi.org/10.1016/0026-0495(93)90129-C
  60. Girola M, Bernardi MD, Contos S. Dose effect in lipid-lowering activity of a new dietary intergrator (chitosan, Garcinia cambogia extract and chromium). Acta Toxicol Therap 1996; 17: 25-40
  61. Preuss HG, Bagchi D, Bagchi M, Rao CV, Dey DK, Satyanarayana S. Effects of a natural extract of (-) -hydroxycitric acid (HCA-SX) and a combination of HCA-SX plus niacin-bound chromium and gymnema sylvestre extract in weight loss. Diabetes Obes Metab 2004; 6: 171-180 https://doi.org/10.1111/j.1462-8902.2004.00328.x