• Title/Summary/Keyword: Dictyostelium

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Distribution of Cellular Slime Molds of Golf Courses in Kyonggi Province (경기도 골프장의 코스별 세포성 점균의 출현분포)

  • 최성영;홍정림;장남기
    • Asian Journal of Turfgrass Science
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    • v.8 no.2
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    • pp.105-110
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    • 1994
  • Dictyostelid cellular slime molds were quantitatively isolated from three golf courses in Kyonggi province. The samples were collected in tee, fairway, rough and out of bounds at each golf courses. Dictyostelium muoroides was found at rough of Seo-Seoul C.C and D. minutum at out of hounds of New Korea C.C Compared with other forests, species diversity and species richness appeared to be low. The properties of soils were investigated in three golf courses. This results were very low in water content, organic matter compared with artificial Korean lawngrsss turf in Seoul National University.

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Effect of a PI3K inhibitor LY294002 on cell migration (세포 이동에서 PI3K 억제제인 LY294002의 효과)

  • Kim, Wonbum;Jeon, Taeck Joong
    • Journal of Integrative Natural Science
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    • v.15 no.3
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    • pp.131-136
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    • 2022
  • Cell migration is essential for diverse cellular processes including wound healing, immune response, development, and cancer metastasis. Pi3-kinase (PI3K) is a key regulator for actin cytoskeleton and phosphorylates phosphatidylinositol (4,5)-diphosphate (PIP2) to phosphatidylinositol (3,4,5)-trisphosphate (PIP3). High levels of PIP3 by PI3Ks are associated with increased levels of F-actin and pseudopod extension at the leading edge of migrating cells such as neutrophils and Dictyostelium. LY294002 is a well-known PI3K specific inhibitor. Here, we investigated the effect of LY294002 on cell migration. First, we evaluated the appropriate concentration of dimethyl sulfoxide (DMSO) for using as a solvent for LY294002. DMSO is a highly polar organic reagent and one of the most common solvent for organic and inorganic chemicals. Cell morphology and cell migration were unaffected at the concentrations less than 0.1 % DMSO. Therefore, stock solution of LY294002 was prepared so that the final concentration of DMSO was 0.1 % or less when treated. When cells were treated with LY294002, cell migration was increased in a concentration-dependent manner. The maximum speed was detected in the presence of 30 µM LY294002. These results suggest that PI3Ks play a inhibitory role in regulating cell migration in our experimental conditions.

Cellular slime molds of mt. halla(II)-occurrence and distribution in the warm temperate region- (漢拏山의 細胞性 粘菌 ( 2 ) - 난온대 지역에서의 출현과 분포 -)

  • Hong, Jeong-Soo;Kwon, Hye-Ryun;Chang, Nam-Kee
    • The Korean Journal of Ecology
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    • v.15 no.2
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    • pp.191-200
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    • 1992
  • Dictyostelid cellular slime molds isolated quantitatibly from the oils of evergreen broad-leaved forest and moist grassland of mt. halla in korea. nineteen species were found, including four undescribed species. species underscribed in korea are dictyostelium aureum var. aureum, d. crassicaule, d. monochasioides, and polysphondylium tenuissimum. Among these isolates, d. crassicaule and d. monochasioides were found only in evergreen broad-leaved forest, and p.tenuissimum was commonly found in moist grassland and meadow. in warn temperate regions investigated, d. mucoroides occurred rarely. It was noticeadle that d. sphaerocephalum, d. aureum var. aureum and d. purpureum were much more commonly found in warm temperate region. Species diversity in evergreen broad-leaved forest was the highest among the forests investigated until now in korea. Many isolates in mt. halla distributed differently in relation to the vegetations and altitudes. distritution patterns of isolates from mt. halla were discussed.

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Occurrence and Distribution of Cellular Slime Molds in Relation to the Coastal Plant Communities of Islands near Inch`on (仁川 近海 島嶼地域의 海岸植物 群落에 따른 細胞性 粘菌의 出現과 分布)

  • Hong, Jeong-Soo;Nam-Kee Chang
    • The Korean Journal of Ecology
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    • v.14 no.4
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    • pp.457-467
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    • 1991
  • Occurrence and distribution of the celluar slime molds in relation to the coastal plant communities of svven islands near inch'on wereinvestigated. as a results, total seven species were isolated from the soils of the coastal plant communities. These are dictyostelium mucoroides, polysphodylium pallidum, dictystelium polycephalum. d. mucoroides was the most commonly found in the coastal plant communities investigated, and was dominant pecularly in the coastal mixed forests, the coastal broad-leaved forests and salt marshes. In the coastal coniferous forest and the coastal dune sand plants, hoeever, p. violaceum was the dominant species. species diversity was relatively was relatively high in the coastal coniferous ferests and the coastal mixed forests. However, agerage number of species isolated from all plant communities was very low,2.8.

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Dual TORCs driven and B56 orchestrated signaling network guides eukaryotic cell migration

  • Kim, Lou W.
    • BMB Reports
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    • v.50 no.9
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    • pp.437-444
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    • 2017
  • Different types of eukaryotic cells may adopt seemingly distinct modes of directional cell migration. However, several core aspects are regarded common whether the movement is either ameoboidal or mesenchymal. The region of cells facing the attractive signal is often termed leading edge where lamellipodial structures dominates and the other end of the cell called rear end is often mediating cytoskeletal F-actin contraction involving Myosin-II. Dynamic remodeling of cell-to-matrix adhesion involving integrin is also evident in many types of migrating cells. All these three aspects of cell migration are significantly affected by signaling networks of TorC2, TorC1, and PP2A/B56. Here we review the current views of the mechanistic understanding of these regulatory signaling networks and how these networks affect eukaryotic cell migration.

A Study on the Distribution and the Effect of Torreya Fruit Extract on Cellular Slime Molds in Torreya Forest of Cheju Island (제주도 비자림에서의 세포성 점균의 분포 및 비자열매 추출액의 성장 효과에 관한 연구)

  • 최선영;장남기
    • Asian Journal of Turfgrass Science
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    • v.10 no.3
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    • pp.187-194
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    • 1996
  • Dictyostelid cellular slime molds were isolated from soils and harks of the subtropical forest of Torreya nucifera in Cheju island. The results were as follows: Polysphondylium pallidum,Dictyostelium purpureum, D. mucoroides, D. aureo-stipes var. aureo-stipes, D. flavidum, D. miniutum, P.violaceu m, D. monocrhasioides. D. brefeldianum, D. polvcephalum. D. areum var. areum, P. tenuissimum, D. fasciculatum. In this forest, P. pallidum and D. purpureum were occurred dominantly, and D. mucoroides and D. aureo-stipes var, aureo-stipes were the second dominant. It was distinguished that D. purpureum was much more cornmonly found than the other forests. Cellular slime molds from the barks of the tree heights of 1,3 and 5m were occured 7, 3 and 2 species respectively. Torreya fruit extract affected on the growth of D. aureo-stipes var. aureo-stipes and D. flavidum but not on D. purpureum and D. mucoroides. Key words: Cellular slime mold, Torreya forest, Torreya fruit extract.

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The Occurrence and Morphological Comparison of Dictyostelid Cellular Slime Molds in Mt. Muhak Soils

  • Hwang, Ji-Young;Hiromitsu Hagiwara;Kim, Jong-Hee
    • The Korean Journal of Ecology
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    • v.23 no.4
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    • pp.315-321
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    • 2000
  • The occurrence and distribution of Dictyostelid cellular slime molds on Mt. Mukak soils with two different vegetation types were investigated. Two plating methods were used for the isolation of dictyostelids following Dr. Hagiwara's method. Dictyostelium and Polysphondylium were isolated in these soils. D. purpureum (subtropical form ) and D. giganteum were observed in both Quercus variabilis and Pinus thunbergii communities soils. D. delicatum, B. sp-1 (D. brefeldianum complex). D. sp-2 (D. brefeldianum complex), D. minutum and P. pallidum complex occurred only in Q. variabilis soil. D. macrocephalum, D. purpureum (temperate form ), D. robustum, D. polycephalum, P. violaceum, and P. pallidium occurred only in P. thunbergii soil. P. pallidium complex is being identified.

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Distribution and New Species of Dictyostelids in Subalpine Zone of Mt. Paektu, Korea

  • Kang, Kyoung-Mi;Chang, Nam-Kee
    • The Korean Journal of Ecology
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    • v.23 no.4
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    • pp.331-337
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    • 2000
  • Fourteen different taxa of dictyostelid cellular slime molds were recovered from the alpine and subalpine zone in Mt. Paektu. In subalpine zone, six species were recovered : Dictyostelium minutum, D. brefeldianum, D. crassicaule, D. capitatum, Polysphondylium solitarium and P. pallidum. One of these species which were isolated from the soils of Larix olgensis community exhibited several distinctive features which differed from the published species. This species was designated as a new species, Polysphondylium solitarium Kang et Chang, sp. nov. When cultivated at 22~24$^{\circ}C$ on low-nutrient agar media with Escherichia coli, Polysphondylium solitarium is distinguishable from other species by the following combination of features: (ⅰ) the sorocarps with vinaceous pigmentation; (ⅱ) pseudoplasmodia radial. usually centralized and rarely subdivided; (ⅲ) the various number of whorls; (ⅳ) the spores with unconsolidated and nonconspicuous polar granules. Also, we confirmed this new species by analyzing ribosomal DNA (ITS1, ITS2 and 5.8s DNA) sequences of P. solitarium and P. violaceum.

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Occurrence and Distribution of Cellular Slime Molds in Mt. Nam (남산에서 세포성 점균의 출현과 분포)

  • 강경미;홍영빈;이재봉;장남기
    • The Korean Journal of Ecology
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    • v.21 no.5_3
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    • pp.687-694
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    • 1998
  • In this study, the occurrence and distribution of dictyostelid cellular slime molds was investigted from soils of typical forests in Mt. Nam and the effect of soil environmental factor on cellular slime molds was investigated. The fourteen species including two undescribed species were isolated as follows: Dictyostelium brefeldianum, Polysphondylium pallidum, P. violaceum, P. pseudo-candidum in Quercus mongolica-Sorbus alnifolia forests, D. purpureum, D. mucoroides var. stoloniferum, D. dimigraformum, D. brefeldiamum, P. pallidum, P. tenuissimum, P. violoceum, P. candidum, P. pseudo-candidum in Pinus densiflora forests, D. polycephahum, D. capitatum, d. brefeldianun, P. candidum in Robinia pseudo-acacia forests, D. purpureum, D. aureostipes var, aureostipes, D. polycephalum in Quercus acutissima forests, D. minutum, D. implicatum. in the site disturbed by human. The dominant species were P. pallidum, D. brefeldiamum, P. pseudo-candidum and D. dimigraformum were the undescribed species in Korea. Environmental factors such as soil pH, water content, organic content, total nitrogen and total phosphorus made a little effect on total species number, the number of clones.

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