• Title/Summary/Keyword: Di-gamma function

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Expression of Δ-desaturase Gene in a Recombinant Pichia pastoris GS115 Strain and Its Activity (재조합 Pichia pastoris GS115에서 Δ-desaturase의 발현과 그 활성)

  • Bae, Kyung-Dong
    • KSBB Journal
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    • v.26 no.6
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    • pp.557-560
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    • 2011
  • It has been known that Δ-desaturase (TAD5) in the biosynthetic pathway of long chain polyunsaturated fatty acids of Thraustochytrium aureumis responsible for the conversion of di-homo-${\gamma}$-linolenic acid (C20:4) into arachidonic acid (C20:4). The genetic sequence analysis on TAD5 of Thraustochytrium aureum ATCC34304 used in this study showed that it has two amino acid changes when compared to that of Thraustochytrium aureum TAD5 first reported in 2003. Accordingly, Thraustochytrium aureum ATCC34304 TAD5 was named TAD5_1. TAD5_1-inserted methylotropic Pichia pastoris was prepared and then cultured with a precursor fatty acid, di-homo-${\gamma}$-linolenic acid. GC analysis confirmed that a certain amount of the precursor fatty acid was converted into arachidonic acid. In this study, not only a recombinant Pichia pastoris with the typical activity of ${\Delta}5$-desaturase which plays an essential role in the biosynthesis of LCPUFAs was successfully made but also the preparationpotential of a recombinant Pichia pastoris strain which may synthesize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) that are important in maintaining and improving human's brain function was proposed.

FORMULAS AND RELATIONS FOR BERNOULLI-TYPE NUMBERS AND POLYNOMIALS DERIVE FROM BESSEL FUNCTION

  • Selin Selen Ozbek Simsek;Yilmaz Simsek
    • Communications of the Korean Mathematical Society
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    • v.38 no.4
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    • pp.1175-1189
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    • 2023
  • The main purpose of this paper is to give some new identities and properties related to Bernoulli type numbers and polynomials associated with the Bessel function of the first kind. We give symmetric properties of the Bernoulli type numbers and polynomials. Moreover, using generating functions and the Faà di Bruno's formula, we derive some new formulas and relations related to not only these polynomials, but also the Bernoulli numbers and polynomials and the Euler numbers and polynomials.

Likelihood ratio in estimating gamma distribution parameters

  • Rahman, Mezbahur;Muraduzzaman, S. M.
    • Journal of the Korean Data and Information Science Society
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    • v.21 no.2
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    • pp.345-354
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    • 2010
  • The Gamma Distribution is widely used in Engineering and Industrial applications. Estimation of parameters is revisited in the two-parameter Gamma distribution. The parameters are estimated by minimizing the likelihood ratios. A comparative study between the method of moments, the maximum likelihood method, the method of product spacings, and minimization of three different likelihood ratios is performed using simulation. For the scale parameter, the maximum likelihood estimate performs better and for the shape parameter, the product spacings estimate performs better. Among the three likelihood ratio statistics considered, the Anderson-Darling statistic has inferior performance compared to the Cramer-von-Misses statistic and the Kolmogorov-Smirnov statistic.

Neutron and gamma-ray energy reconstruction for characterization of special nuclear material

  • Clarke, Shaun D.;Hamel, Michael C.;Di fulvio, Angela;Pozzi, Sara A.
    • Nuclear Engineering and Technology
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    • v.49 no.6
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    • pp.1354-1357
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    • 2017
  • Characterization of special nuclear material may be performed using energy spectroscopy of either the neutron or gamma-ray emissions from the sample. Gamma-ray spectroscopy can be performed relatively easily using high-resolution semiconductors such as high-purity germanium. Neutron spectroscopy, by contrast, is a complex inverse problem. Here, results are presented for $^{252}Cf$ and PuBe energy spectra unfolded using a single EJ309 organic scintillator; excellent agreement is observed with the reference spectra. Neutron energy spectroscopy is also possible using a two-plane detector array, whereby time-of-flight kinematics can be used. With this system, energy spectra can also be obtained as a function of position. Spatial-dependent energy spectra are presented for neutron and gamma-ray sources that are in excellent agreement with expectations.

Isolation and Phylogenetic Characterization of Chitinase Producing Oligotrophic Bacteria (Chitinase생산 저영양세균의 분리 및 계통분류학적 특성)

  • Kim Soo-Jin;Kim Min-Young;Koo Bon-Sung;Yoon San-Hong;Yeo Yun-Soo;Park In-Cheol;Kim Yoon-Ji;Lee Jong-Wha;Whang Kyung-Sook
    • Korean Journal of Microbiology
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    • v.41 no.4
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    • pp.293-299
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    • 2005
  • Many isolates from soil of Korean ginseng rhizosphere did not show remarkable growth on full strength of the conventional nutrient broth (NB medium) but grew on its 100-fold dilution (DNB medium). Six hundred-forty strains were isolated as oligotrophic bacteria. In the course of screening for new bioactive compounds from oligotrophic bacteria from soil, 8 strains which had appeared to form of clear zone on a medium containing colloidal chitin as a sole carbon source were selected for further studies. Strain CR42 hydrolyzed a fluorogenic analogue of chitin, 4-methylumbelliferyl-D-glucosaminide (MUF-NAG) . Mo st of the culture supernatant of these isolates hydrolyzed 4-methylumbelliferyl-D-N,N'-diacetylchitobioside (MUF-diNAG). The isolates were heterogeneous and categorized to gamma- and beta-proteobacteria, Bacillaceae, Actinobactepia, and Bacteroides by 16S rRNA analysis. Two strains, WR164 and CR18, had a 16S rRNA sequence of $95-96\%$ identical to uncultured bacteria. It was observed that CR2 and CR75 could inhibit the growth of Colletotrichum gloeosporioides with hyphal extention-inhibition assay on PDA plate supplemented with $1\%$ colloidal chitin.

A high-density gamma white spots-Gaussian mixture noise removal method for neutron images denoising based on Swin Transformer UNet and Monte Carlo calculation

  • Di Zhang;Guomin Sun;Zihui Yang;Jie Yu
    • Nuclear Engineering and Technology
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    • v.56 no.2
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    • pp.715-727
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    • 2024
  • During fast neutron imaging, besides the dark current noise and readout noise of the CCD camera, the main noise in fast neutron imaging comes from high-energy gamma rays generated by neutron nuclear reactions in and around the experimental setup. These high-energy gamma rays result in the presence of high-density gamma white spots (GWS) in the fast neutron image. Due to the microscopic quantum characteristics of the neutron beam itself and environmental scattering effects, fast neutron images typically exhibit a mixture of Gaussian noise. Existing denoising methods in neutron images are difficult to handle when dealing with a mixture of GWS and Gaussian noise. Herein we put forward a deep learning approach based on the Swin Transformer UNet (SUNet) model to remove high-density GWS-Gaussian mixture noise from fast neutron images. The improved denoising model utilizes a customized loss function for training, which combines perceptual loss and mean squared error loss to avoid grid-like artifacts caused by using a single perceptual loss. To address the high cost of acquiring real fast neutron images, this study introduces Monte Carlo method to simulate noise data with GWS characteristics by computing the interaction between gamma rays and sensors based on the principle of GWS generation. Ultimately, the experimental scenarios involving simulated neutron noise images and real fast neutron images demonstrate that the proposed method not only improves the quality and signal-to-noise ratio of fast neutron images but also preserves the details of the original images during denoising.

Curie Temperature Transition According to Microstructure of Polymer Chain in Poly(VDF/TrFE/CTFE) Terpolymer (Poly(VDF/TrFE/CTFE) 3성분계 고분자의 배열구조에 따른 상전이 온도의 변화)

  • Kim, Eun-Kyoung;Lee, Sang-Goo;Ha, Jong-Wook;Park, In-Jun;Lee, Soo-Bok;Park, Cheol-Min;Kim, Young-Ho
    • Polymer(Korea)
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    • v.31 no.4
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    • pp.343-348
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    • 2007
  • In this study, terpolymer of vinylidene fluoride (VDF), trifluoroethylene (TrFE), and chlorotrifluoroethylene (CTFE) were prepared by suspension polymerization using di-tertiary-butyl peroxide (DTBP) as an initiator. The structural characteristics including microstructure and chain conformation of the polymers have been carefully elucidated as a function of the chemical composition using NMR, FT-IR. The intensity of absorption bands of the $\beta$-phase gradually decreases and the $\gamma$-phase increases with the increment of CTFE mol%. The analysis results of DSC shows that the Curie phase transition temperature ($T_c$) of the terpolymer gradually shifts to ambient temperature and trace becomes smaller and broader with the increment of CTFE mol%. Also, activation energies of the samples were calculated by Freeman-Carroll method.

Hepatoprotective effect of Ainsliaea acerifolia water extract on LPS/D-GalN-induced acute liver injury in human HepG2 cells (단풍취 열수 추출물의 HepG2 인간간세포의 LPS/D-Gal에 의해 유발된 급성 간 손상에 대한 간보호 효과)

  • Lee, Sun-Yeop;Han, Joon-Hee;Choi, Da-Hye;Hong, Min;Kwon, Tae-Hyung;Lee, Yong-Jin;Yu, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.476-481
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    • 2020
  • This study investigated the antioxidant and hepatoprotective effects of Ainsliaea acerifolia water extract (AAWE) on HepG2 cells. Five types of caffeoylquinic acid (CQA) were detected in AAWE, namely, 4,5-di-O-caffeoylquinic acid (4,5-DCQA; 11.16 mg/g), 3,4-di-O-caffeoylquinic acid (3,4-DCQA; 5.23 mg/g), 5-O-caffeoylquinic acid (5-CQA; 4.88 mg/g), 3,5-di-O-caffeoylquinic acid (3,5-DCQA; 3.51 mg/g), and 4-O-caffeoylquinic acid (4-CQA; 3.31 mg/g). AAWE exerted ABTS+ antioxidant effects, evidenced by polyphenol content and 2,2'2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH radical scavenging) activities. AAWE (300 ㎍/mL) treatment significantly decreased the activities of gamma glutamyl transferase (GGT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) as compared to control and exerted protective effects against the increase in liver function index induced by lipopolysaccharide (LPS)/galactosamine (D-GalN) in HepG2 cells. In addition, the secretion of tumor necrosis factor (TNF)-α by HepG2 cells induced by LPS/D-GalN significantly increased in all treatment groups compared to that in the control. However, AAWE (100-300 ㎍/mL) treatment significantly decreased the secretion of TNF-α compared to that in the control. These results suggest that AAWE treatment reduces hepatotoxicity by increasing antioxidant activities, reducing GGT, AST, and LDH activities, and inhibiting TNF-α secretion.

Alteration of Phospholipase D Activity in the Rat Tissues by Irradiation (방사선 조사에 의한 쥐 조직의 포스포리파제 D의 활성 변화)

  • Choi Myung Sun;Cho Yang Ja;Choi Myung-Un
    • Radiation Oncology Journal
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    • v.15 no.3
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    • pp.197-206
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    • 1997
  • Purpose : Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid (PA) and choline. Recently, PLD has been drawing much attentions and considered to be associated with cancer Process since it is involved in cellular signal transduction. In this experiment, oleate-PLD activities were measured in various tissues of the living rats after whole body irradiation. Materials and Methods : The reaction mixture for the PLD assay contained $0.1\;\muCi\;1,2-di[1-^{14}C]palmitoyl$ phosphatidylcholine 0.5mM phosphatidylcholine, 5mM sodium oleate, $0.2\%$ taurodeoxycholate, 50mM HEPES buffer(pH 6.5), 10mM $CaCl_2$, and 25mM KF. phosphatidic acid, the reaction product, was separated by TLC and its radioactivity was measured with a scintillation counter. The whole body irradiation was given to the female Wistar rats via Cobalt 60 Teletherapy with field size of 10cmx loom and an exposure of 2.7Gy per minute to the total doses of 10Gy and 25Gy. Results : Among the tissues examined, PLD activity in lung was the highest one and was followed by kidney, skeletal muscle, brain, spleen, bone marrow, thymus, and liver. Upon irradiation, alteration of PLD activity was observed in thymus, spleen, lung, and bone marrow. Especially PLD activities of the spleen and thymus revealed the highest sensitivity toward $\gamma-rar$ with more than two times amplification in their activities In contrast, the PLD activity of bone marrow appears to be reduced to nearly $30\%$. Irradiation effect was hardly detected in liver which showed the lowest PLD activity. Conclusion : The PLD activities affected most sensitively by the whole-body irradiation seem to be associated with organs involved in immunity and hematopoiesis. This observation s1ron91y indicates that the PLD is closely related to the physiological function of these organs, Furthermore, radiation stress could offer an important means to explore the phenomena covering from cell Proliferation to cell death on these organs.

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