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Isolation and Phylogenetic Characterization of Chitinase Producing Oligotrophic Bacteria  

Kim Soo-Jin (Microbial Function Team, National Institute of Agricultural Biotechnology(NIAB))
Kim Min-Young (Microbial Function Team, National Institute of Agricultural Biotechnology(NIAB))
Koo Bon-Sung (Microbial Function Team, National Institute of Agricultural Biotechnology(NIAB))
Yoon San-Hong (Microbial Function Team, National Institute of Agricultural Biotechnology(NIAB))
Yeo Yun-Soo (Microbial Function Team, National Institute of Agricultural Biotechnology(NIAB))
Park In-Cheol (Microbial Function Team, National Institute of Agricultural Biotechnology(NIAB))
Kim Yoon-Ji (Dept. of Biotechnology, Mokwon University)
Lee Jong-Wha (Inst. of Microbial Ecology and Resources)
Whang Kyung-Sook (Dept. of Biotechnology, Inst. of Microbial Ecology and Resources, Mokwon University)
Publication Information
Korean Journal of Microbiology / v.41, no.4, 2005 , pp. 293-299 More about this Journal
Abstract
Many isolates from soil of Korean ginseng rhizosphere did not show remarkable growth on full strength of the conventional nutrient broth (NB medium) but grew on its 100-fold dilution (DNB medium). Six hundred-forty strains were isolated as oligotrophic bacteria. In the course of screening for new bioactive compounds from oligotrophic bacteria from soil, 8 strains which had appeared to form of clear zone on a medium containing colloidal chitin as a sole carbon source were selected for further studies. Strain CR42 hydrolyzed a fluorogenic analogue of chitin, 4-methylumbelliferyl-D-glucosaminide (MUF-NAG) . Mo st of the culture supernatant of these isolates hydrolyzed 4-methylumbelliferyl-D-N,N'-diacetylchitobioside (MUF-diNAG). The isolates were heterogeneous and categorized to gamma- and beta-proteobacteria, Bacillaceae, Actinobactepia, and Bacteroides by 16S rRNA analysis. Two strains, WR164 and CR18, had a 16S rRNA sequence of $95-96\%$ identical to uncultured bacteria. It was observed that CR2 and CR75 could inhibit the growth of Colletotrichum gloeosporioides with hyphal extention-inhibition assay on PDA plate supplemented with $1\%$ colloidal chitin.
Keywords
chitinase; Colletotrichum gloeosporioides; MUF-diNAG; MUF-NAG; oligotrophic bacteria;
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1 Clarke, P.H. and M.V. Tracey. 1956. The occurrence of chitinase in some bacteria. J. Gen. Microbiol. 14, 188-196   DOI   PUBMED   ScienceOn
2 Lane, D.J. 1991. 16S/23S rRNA sequencing, In: Stackebrandt, E., M. Goodfellow (Eds.), Nucleic acid techniques in bacterial systematics, John Wiley and Sons, Chichester. pp.115-175
3 Metcalfe, A.C., M. Krsek, G.W. Gooday, J.I. Prosser, and E.M.H. Wellington. 2002. Molecular analysis of a bacterial chitinolytic community in an upland pasture. Appl. Environ. Microbiol. 68, 5042-5050   DOI   ScienceOn
4 Ohta, H. and T. Hattori. 1980. Bacteria sensitive to nutrient broth medium in terrestrial environments. Soil Sci. Plant Nutr. 26, 99-107   DOI
5 Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425   PUBMED
6 Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680   DOI   ScienceOn
7 Watanabe, T., R. Kanai, T. Tanabe, M. Mitsutomi, S. Sakuda, and K. Miyashita. 1999. Family 19 chitinases of streptomyces species: characterization and distribution. Microbiology 145, 3353-3363   DOI   PUBMED
8 Whang, K. and T. Hattori. 1988. Oligotrophic bacteria in rendzina a forest soil. Antonie van Leewenhoek J. Microbiol. 54, 19-37   DOI   ScienceOn
9 Whang, K. and S. Yu. 1995. Growth patterns of soil bacteria in different organic concentrations, and isolation of facultative and obligate oligotrophic bacteria. The Microorganisms & Industry 21, 319-324
10 Zhu, H., F. Qu, and L. Zhu. 1993. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Res. 21, 5279-5280   DOI   ScienceOn
11 Johnson, J.L. 1994. Simility analysis of rRNAs, In: Gerhardt, P., R.G.E. Murray, W.A. Wood, N.R. Krirg (Eds.), Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC. pp.683-700
12 Omera, C., B.A. Horwitz, and I. Chet. 2001. A convenient fluorometric method for the detection of extracellular N-acetylglucosaminidase production by filamentous fungi. J. Microbiol. Methods. 43, 165-169   DOI   ScienceOn
13 Sakai, D., A. Yokota, H. Kurokawa, M. Wakayama, and M. Moriguchi. 1998. Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. Appl. Environ. Microbiol. 64, 3397-3402   PUBMED
14 오승환, 유연현. 1996. 인삼의 병. 최신고려인삼(재배편), p. 197-241. 한국인삼연초연구소
15 中神照太, 外村健三. 1966. 放線菌の生産するキチィン分 解酵素に關する硏究(第1報)分解菌の 檢索と培養條件につ いて. 工業技術院醱酵硏究所報告. 30, 19-26
16 Ohtakara, A. (1971) The chitinase system of a strain of Streptomyces griceus. Bull. Hiroshima Women's Univ. 6, 1-12
17 Chernin, L.S., M.K. Winson, J.M. Thompson, S. Haran, B.W. Bycroft, I. Chet, P. Williams, and G.S.A.B. Stewart. 1998. Chitinolytic activity in Chromobacterium violaceum: Substrate analysis and regulation by quorum sensing. J. Bacteriol. 180, 4435-4441   PUBMED
18 Monreal, J. and E. T. Reese. 1969. The chitinase of Serratia marcescens. Can. J. Microbiol. 15, 689-696   DOI   PUBMED   ScienceOn
19 Radjacommare, R., A. Ramanathan, A. Kandan, G.V. Sible, S. Harish, and R. Samiyappan. 2004. Purification and anti-fungal activity of chitinase against Pyricularia grisea in finger millet. World J. of Microbiol. Biotechnol. 20, 251-256   DOI   ScienceOn
20 Dempsey, D.M.A., H. Silva, and D.F. Klessig. 1998. Engineering disease and pest resistance in plants. Trends Microbiol. 6, 54-61   DOI   ScienceOn
21 이강표, 김창남, 유주현, 오두환. 1990. Aeromonas salmonicida YA7-625에 의한 chitinase의 생산 및 정제. 한국산업미생물학회지. 18, 599-606
22 Tominaga, Y. and Y. Tsujisaka, 1976. Purification and some properties of two chitinase from Streptomyces orientalis which lyse Rhizopus cell wall. Agric. Biol. Chem. 40, 2325-2333   DOI
23 오승환, 유연현, 김영호, 김기광, 이일호. 1988. 인삼의 재배법 변화에 따른 병해충 발생생태 및 방제연구. 인삼 연구보고서(재배분야, 환경 및 육종분야), p. 3-137. 인삼연초연구소
24 Driss, F., M. Kallassy-Awad, N. Zouari, and S. Jaoua. 2005. Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki. J. Appl. Microbiol. 99, 945-953   DOI   ScienceOn
25 Nikitin, D.I. and K.V. Chumakov. 1985. The functional role of oligotrophic microorganisms. In V. Jensen (ed.), Microbial communities in soil. FEMS Symposium. 33, 177-189
26 Wortman, A.T., C.C. Somerville, and R.R. Cotwell. 1986. Chitinase determinants of Vibrio vulnificus: gene cloning and applications of a chitinase probe. Appl. Environ. Microbiol. 52, 142-145   PUBMED
27 Chernin, L.S., L. Fuente, V. Sobolev, S. Haran, C.E. Vorgias, A.B. Oppenheim, and I. Chet. 1997. Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans. Appl. Environ. Microbiol. 63, 834-839   PUBMED
28 Downing, K.J., G. Leslie, and J.A. Thomson. 2000. Biocontrol of the sugarcane borer Eldana saccharina by expression of the Bacillus thuringiensis cry1Ac7 and Serratia marcescens chiA genes in sugarcane-associated bacteria. Appl. Environ. Microbiol. 66, 2804-2810   DOI   ScienceOn
29 Hoster, F., J.E. Schmitz, and R. Daniel. 2005. Enrichment of chitinolytic microorganism: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Appl. Micriobiol. Biotechnol. 66, 434-442   DOI   ScienceOn
30 Ishida, Y. and H. Kadota. 1979. A new method for enumeration of oligotrophic bacteria in lake water. Arch. Hydrobiol. 12, 77-85
31 Mattew, T.C., N.W. Daniel, Y. Liying, and L.K. David. 2000. Selected chitinase genes in cultured and uncultured marine bacteria in the ${\alpha}$- and ${\beta}$-subclasses of the proteobacteria. Appl. Environ. Microbiol. 66, 1195-1201   DOI   PUBMED
32 Hsu, S.C. and J.L. Lockwood. 1975. Powdered chitin agar as a selective medium for enumeration of Actinomycetes in water and soil. Appl. Microbiol. 29, 422-426   PUBMED
33 Kuzunetsov, S. I., G.A. Dubinnina, and N.A. Lapteva, 1979. Biology of oligotrophic bacteria. Ann. Rev. Microbiol. 33, 377-387   DOI   ScienceOn
34 Felse, P.A. and T. Panda. 1999. Regulation and cloning of microbial chitinase genes. Appl. Microbiol. Biotechnol. 51, 141-151   DOI   ScienceOn
35 Mattew, T.C., A.M. Jessica, and L.K. David. 1999. Chitinase from uncultured marine microorganisms. Appl. Environ. Microbiol. 65, 2553-2557   PUBMED
36 Wang S.L. and W.T. Chang. 1997. Purification and characterization of two bifunctional chitinases/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium. Appl. Environ. Microbiol. 63, 380-386   PUBMED