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http://dx.doi.org/10.9721/KJFST.2020.52.5.476

Hepatoprotective effect of Ainsliaea acerifolia water extract on LPS/D-GalN-induced acute liver injury in human HepG2 cells  

Lee, Sun-Yeop (Department of Research and Development, Chuncheon Bioindustry Foundation)
Han, Joon-Hee (Department of Research and Development, Chuncheon Bioindustry Foundation)
Choi, Da-Hye (Department of Research and Development, Chuncheon Bioindustry Foundation)
Hong, Min (Department of Research and Development, Chuncheon Bioindustry Foundation)
Kwon, Tae-Hyung (Department of Research and Development, Chuncheon Bioindustry Foundation)
Lee, Yong-Jin (Department of Research and Development, Chuncheon Bioindustry Foundation)
Yu, Keun-Hyung (Department of Research and Development, Chuncheon Bioindustry Foundation)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.5, 2020 , pp. 476-481 More about this Journal
Abstract
This study investigated the antioxidant and hepatoprotective effects of Ainsliaea acerifolia water extract (AAWE) on HepG2 cells. Five types of caffeoylquinic acid (CQA) were detected in AAWE, namely, 4,5-di-O-caffeoylquinic acid (4,5-DCQA; 11.16 mg/g), 3,4-di-O-caffeoylquinic acid (3,4-DCQA; 5.23 mg/g), 5-O-caffeoylquinic acid (5-CQA; 4.88 mg/g), 3,5-di-O-caffeoylquinic acid (3,5-DCQA; 3.51 mg/g), and 4-O-caffeoylquinic acid (4-CQA; 3.31 mg/g). AAWE exerted ABTS+ antioxidant effects, evidenced by polyphenol content and 2,2'2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH radical scavenging) activities. AAWE (300 ㎍/mL) treatment significantly decreased the activities of gamma glutamyl transferase (GGT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) as compared to control and exerted protective effects against the increase in liver function index induced by lipopolysaccharide (LPS)/galactosamine (D-GalN) in HepG2 cells. In addition, the secretion of tumor necrosis factor (TNF)-α by HepG2 cells induced by LPS/D-GalN significantly increased in all treatment groups compared to that in the control. However, AAWE (100-300 ㎍/mL) treatment significantly decreased the secretion of TNF-α compared to that in the control. These results suggest that AAWE treatment reduces hepatotoxicity by increasing antioxidant activities, reducing GGT, AST, and LDH activities, and inhibiting TNF-α secretion.
Keywords
Ainsliaea acerifolia; water extract; hepatoprotective effect; LPS/D-GalN;
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Times Cited By KSCI : 2  (Citation Analysis)
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