• Journal of Microbiology and Biotechnology
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• v.34 no.6
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• pp.1348-1355
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• 2024

The eukaryotic translation initiation factor eIF5B is a bacterial IF2 ortholog that plays an important role in ribosome joining and stabilization of the initiator tRNA on the AUG start codon during the initiation of translation. We identified the fluorophenyl oxazole derivative 2,2-dibromo-1-(2-(4-fluorophenyl)benzo[d]oxazol-5-yl)ethanone quinolinol as an inhibitor of fungal protein synthesis using an in vitro translation assay in a fungal system. Mutants resistant to this compound were isolated in Saccharomyces cerevisiae and were demonstrated to contain amino acid substitutions in eIF5B that conferred the resistance. These results suggest that eIF5B is a target of potential antifungal compound and that mutation of eIF5B can confer resistance. Subsequent identification of 16 other mutants revealed that primary mutations clustered mainly on domain 2 of eIF5B and secondarily mainly on domain 4. Domain 2 has been implicated in the interaction with the small ribosomal subunit during initiation of translation. The tested translation inhibitor could act by weakening the functional contact between eIF5B and the ribosome complex. This data provides the basis for the development of a new family of antifungals.

• Journal of The Korean Society of Grassland and Forage Science
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• v.44 no.3
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• pp.197-203
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• 2024

This study investigates the role of the NAC transcription factor ANAC032 in regulating abscisic acid (ABA)-dependent stress responses and its involvement in sugar signaling pathways. Arabidopsis seedlings with overexpressed or knock-out ANAC032 were examined for their sensitivity to ABA, glucose, and fluridone to elucidate the functional role of ANAC032 in ABA and high glucose-mediated growth retardation. Our results showed that ANAC032 negatively regulates ABA responses, as ANAC-overexpressing plants exhibited higher ABA sensitivity, while anac032 mutants were less sensitive. Under high glucose conditions, anac032 mutants demonstrated hyposensitivity, with germination rates higher than wild-type and ANAC032-overexpressing plants. Additionally, yeast two-hybrid screening identified three NAC proteins, ANAC020, ANAC064, and ANAC074, interact with ANAC032. These findings highlight ANAC032's role in stress signaling pathways and its potential interactions with other NAC proteins, contributing to a better understanding of transcriptional regulation in plant stress responses and possibly expanding to forage crop development.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.407-416
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• 2019

A common post-harvest disease of sweetpotato tuber is root rot caused by Fusarium solani in Korea as well as the other countries. Storage root rot disease was monitored earlier on sweetpotato (Ipomoea batatas) in storehouses of different locations in Korea. In the present study, an isolate SPL16124 was choosen and collected from Sweetpotato Research Lab., Bioenergy Crop Research Institute, NICS, Muan, Korea, and confirmed the identification as Fusarium solani by conidial and molecular phylogenetic analysis (internal transcribed spacer ITS and translation elongation factor EF 1-α gene sequences). The isolate was cultured on potato dextrose agar, and conidiation was induced. The fungus was screened for Fusarium root rot on tuber of 14 different varieties. Among the tested variety, Yenjami, Singeonmi, Daeyumi, and Sinjami showed resistant to root rot disease. Additionally, the pathogen was tested for pathogenicity on stalks of these varieties. No symptom was observed on the stalk, and it was confirmed that the disease is tissue specific.

• Journal of the Microelectronics and Packaging Society
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• v.23 no.4
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• pp.43-48
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• 2016

3-Dimensional System in Package (3-D SiP) structure (Amkor calls it mPossum-molded Possum) using double side Surface Mount Technology (SMT) and double side molding was evaluated in order to achieve small/thin form factor as well as good functionality by integration and double side layout. As the new platform on laminate substrate basis, molding process was challenge in mold flow balance at top and bottom side and package warpage control over the overall assembly process. There were two types of different molding process evaluated with 1) 1-step molding which was done at both side at the same time and 2) 2-step molding which was done at the conventional molding process twice. Mold simulation helped to narrow down the material selections and parameters available before actual sample build. There were many challenges for this first trial in design/ parameter and material types but optimized them to enable this structure.

• Human Ecology Research
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• v.52 no.1
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• pp.43-53
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• 2014

The purpose of this study was to 1) investigate the characteristics of burnout of Korean elementary school students and 2) develop and validate the Korean Academic Burnout Scale-Elementary School Students (KABS-ESS). Fifty-one behavioral descriptions of burnout extracted through a preliminary survey, focus group interview, and literature review were administered to a sample of 531 elementary students of the 4th to 6th grade in Gyeonggi Province. The results of an exploratory factor analysis indicated that the structure of the burnout of Korean elementary school students consists of seven factors (i.e., inefficacy, avoidance, cynicism, emotional exhaustion, physical exhaustion, antipathy and cognitive exhaustion), and this structure of final 28 items was confirmed by the confirmatory factor analysis. The KABS-ESS showed a correlation of .771 with a representative academic burnout scale, Maslach Burnout Inventory-Student Survey, and -.561 with the academic engagement scale, indicating that both convergent and discriminant validities were confirmed. Furthermore, all fit indices of the structural equation model of the Schaufeli and Bakker's job demands-resources in an academic setting were satisfactory, and the possibility of the validity generalization of the model was supported. The demand variables predicting academic burnout and engagement were academic overload and parental pressure, while self-resilience, self-regulated learning strategies, and parental and the teacher's support were identified as the resource variables. Lastly, the implications and future direction of the present study were discussed.

• The Journal of Korean Association of Computer Education
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• v.23 no.3
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• pp.17-30
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• 2020

Although the importance of Artificial Intelligence(AI) education is increasing, research on the development of test tools has not been conducted in AI education research in Korea has not been conducted. Accordingly, there is a limit to designing AI curriculum and analyzing the effect of the educational program. So, in this study, Test tool was developed that to measure the attitude toward artificial intelligence of middle school students. For the development of test tools, the objectives, components, factors, and test tools were developed through the discussion of AI education experts. The test tool was finally developed by item analysis, exploratory factor analysis and confirmatory factor analysis. Finally, the criteria of the developed test tool were analyzed. Future research is needed to analyze the effects of educational programs and to analyze factors affecting attitudes toward artificial intelligence in middle school students using developed test tools.

• Journal of the Korean Ceramic Society
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• v.42 no.9 s.280
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• pp.631-636
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• 2005

$LiNi_{1-y}Ga_yO_2$ (y = 0.005, 0.010, 0.025, 0.050, and 0.100) were synthesized by the solid-state reaction method after mechanical mixing, and their_electrochemical properties were investigated. All the $LiNi_{1-y}Ga_yO_2$ (y=0.005, 0.010, 0.025, 0.050, and 0.100) samples had the R3m structure. The sample with y = 0.025 showed the largest first discharge capacity (131.4 mAh/g) and good cycling performance [discharge capacity 117.5 mAh/g ($89.4{\%}$ of the first discharge capacity) at the 20th cycle]. The first discharge capacity decreased as the value of y increased. The samples with y = 0.010 and y = 0.005 had small R-factor but their cycling performance was worse than that of the sample with y = 0.025. All the $LiNi_{1-y}Ga_yO_2$ samples had smaller discharge capacities than $LiNiO_2$, but their cycling performances were better than that of $LiNiO_2$.

• Radiation Oncology Journal
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• v.30 no.2
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• pp.62-69
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• 2012

Purpose: To evaluate the treatment outcome and prognostic factor of postoperative radiotherapy for extremity soft tissue sarcoma (STS). Materials and Methods: Forty three patients with extremity STS were treated with conservative surgery and postoperative radiotherapy from January 1981 to December 2010 at Korea University Medical Center. Median total 60 Gy (range, 50 to 74.4 Gy) of radiation was delivered and 7 patients were treated with chemotherapy. Results: The median follow-up period was 70 months (range, 5 to 302 months). Twelve patients (27.9%) sustained relapse of their disease. Local recurrence occurred in 3 patients (7.0%) and distant metastases developed in 10 patients (23.3%). The 5-year overall survival (OS) was 69.2% and disease free survival was 67.9%. The 5-year local relapse-free survival was 90.7% and distant relapse-free survival was 73.3%. On univariate analysis, no significant prognostic factors were associated with development of local recurrence. Histologic grade (p = 0.005) and stage (p = 0.02) influenced the development of distant metastases. Histologic grade was unique significant prognostic factor for the OS on univariate and multivariate analysis. Severe acute treatment-related complications, Common Terminology Criteria for Adverse Events (CTCAE) grade 3 or 4, developed in 6 patients (14.0%) and severe late complications in 2 patients (4.7%). Conclusion: Conservative surgery with postoperative radiotherapy achieved a satisfactory rate of local control with acceptable complication rate in extremity STS. Most failures were distant metastases that correlate with tumor grade and stage. The majority of local recurrences developed within the field. Selective dose escalation of radiotherapy or development of effective systemic treatment might be considered.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.11
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• pp.1532-1536
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• 2015

To verified the target genes of miR-34c, bioinformatics software was used to predict the targets of miR-34c. Three possible target genes of miR-34c related to spermatogenesis and male reproductive development: zinc finger protein 148 (ZNF148), kruppel-like factor 4 (KLF4), and platelet-derived growth factor receptor alpha (PDGFRA) were predicted. Then, the expression of miR-34c and its target genes were detected in swine testicular tissue at different developmental stages by quantitative polymerase chain reaction. The results suggested that the expression of PDGFRA has the highest negative correlation with miR-34c. Then immunohistochemical staining was done to observe the morphology of swine testicular tissue at 2-days and 3, 4, 5-months of age, which indicated that PDGFRA was mainly expressed in the support cells near the basement membrane during the early development stages of testicular tissue, but that the expression of PDGFRA was gradually reduced in later stages. Therefore, western blot analyzed that the highest expression of PDGFRA was generated in 2-days old testicular tissues and the expression levels reduced at 3 and 4-months old, which correlated with the results of immunohistochemical staining. In conclusion, PDGFRA is a target gene of miR-34c.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.8
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• pp.2853-2859
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• 2010

Development and promotion of foreign tourism products is an important factor for economical side as well as to improving the foreign relation and the image of nation. Also, foreign tourism products can give a positive effect in activation of the domestic tourism industry and foreigner's domestic visit. Thus, development and promotion of travel industry may offer competitive power of the specification country and a development opportunity, and influence in investment infancy of different industry. Therefore, in this study, service quality of this foreign tourism products analyze effect about customer satisfaction and re-purchase intention, investigated competitive power factors of the tourism industry in the future. In the final study, service quality patten, confidence, attraction could be influenced on customer satisfaction, reliability and responsiveness was not affected by the other factors. This study may suggest guideline that analyze the special character factors of the tourism industry future, or develop the critical success factor of foreign tourism products.