This experiment was conducted to evaluate the main effects and pleiotropic effects of two comparable isogenic lines for culm and spike length in hulled barley and observed various agronomic characteristics, yield and yield components under the conditions of heavy fertilizer application and high planting densities. Three isogenic lines, culm, spike and culm and spike, were planted and to obtain basic data for improving high-yielding and quality of hulled barley at Experiment Farm, Dankook Univ., Cheonan city in 1985. Differences of culm length between two comparable isogenic lines, short culmed line (SCL) and medium culmed line (MCL), in isogenic lines for culm length showed highly significant differences. And differences of spike length between two comparable isogenic lines, short spike line (SSL) and medium spike line (MSL), in isogenic lines for spike length showed highly significant differences. Differences of culm and spike length between two comparable isogenic lines, short culmed and spike line (SCSSL) and medium culmed and spike line (MCMSL), short culmed and spike line (SCSSL) and long culmed and spike line (LCLSL), in isogenic lines for culm and spike length showed highly significant differences. Characteristics affected highly by the genes of culm length in isogenic lines for culm length were heading days, the 1st and 2nd internode length, No. of grains per spike, No. of spikes/m$^2$, and one litre weight. Characteristics affected highly by the genes of spike length in isogenic lines for spike length were No. of spike nodes, heading days, maturity days, thousand grain weight and one litre weight. Characteristics affected highly by the genes of culm and spike length in isogenic lines for culm and spike length were No. of spike nodes, the 2nd, 3rd and 4th internode length, thousand grain weight and grain yield. Grain yield of SCL, MSL, MCMSL and SCSSL was more than grain yield of MCL, SSL, SCSSL and LCLSL, respectively. Maturity days of SCL, MSL, SCSSL and LSLSL were faster than maturity days of MCL, SSL, MCMSL and SCSSL, respectively.