식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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미끼식물을 이용한 화상병 감염 기주 매몰지 내 화상병균 제거 효율 검증 및 병 재발 모니터링

Monitoring the Reoccurrence of Fire Blight and the Eradication Efficiency of Erwinia amylovora in Burial Sites of Infected Host Plants Using Sentinel Plants

  • 박인웅 (서울대학교 식물면역연구센터) ;
  • 송유림 (경희대학교 원예생명공학과) ;
  • 응우옌 트렁 부 (경희대학교 그린바이오과학원) ;
  • 오엄지 (경희대학교 그린바이오과학원) ;
  • 황인선 (경희대학교 원예생명공학과) ;
  • 함현희 (국립농업과학원 작물보호과) ;
  • 김성환 (단국대학교 생명과학부 미생물전공) ;
  • 박덕환 (강원대학교 생물자원과학부 응용생물학전공) ;
  • 오창식 (서울대학교 식물면역연구센터)
  • In Woong, Park (Plant Immunity Research Center, Seoul National University) ;
  • Yu-Rim, Song (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Nguyen Trung, Vu (Graduate School of Green-Bio Science, Kyung Hee University) ;
  • Eom-Ji, Oh (Graduate School of Green-Bio Science, Kyung Hee University) ;
  • In Sun, Hwang (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Hyeonheui, Ham (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong Hwan, Kim (School of Life Sciences, Dankook University) ;
  • Duck Hwan, Park (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Chang-Sik, Oh (Plant Immunity Research Center, Seoul National University)
  • 투고 : 2022.11.30
  • 심사 : 2022.12.22
  • 발행 : 2022.12.31
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초록

Erwinia amylovora에 의해 발생하는 화상병은 2015년에 국내에서 처음으로 보고된 이후 2021년 기준으로 전국 22개 지역으로 전파되었다. 우리나라는 식물방역법에 따라 화상병이 발생한 사과 및 배 과원은 발생주율을 기준으로 모든 기주식물들을 완전히 제거하여 구덩이에 매몰처리한다. 이후, 3년간 화상병균 전파를 막기 위해 매몰지 위에 기주식물 식재를 금하고 있다. 매몰처리법에 의한 화상병균 박멸 효과를 확인하기 위하여, 화상병 감수성 식물을 미끼식물로 이용하여 매몰지에 식재 후 화상병 재발 유무를 확인하였다. 이를 위해, 2019년부터 2021년에 매몰처리한 경기도 안성시와 충북 충주시 소재 매몰지 3곳을 선정하여 미끼식물 감시시설을 설치하였다. 화상병 감수성 식물인 사과(부사)를 미끼식물로 선정하고, 각 감시시설당 5주를 식재하였다. 감시시설은 울타리와 그물로 격리하였다. 또한, 실시간 모니터링을 위한 CCTV와 동작감지기, 그리고 현지 기상상황을 기록하는 센서를 설치하였다. 감시시설을 주기적으로 방문하여 육안으로 화상병 발병 유무를 확인하였다. 미끼식물로부터 화상병 의심 증상을 나타내는 표본을 채취하고 화상병균 특이적 프라이머를 이용하여 loop-mediated isothermal amplification polymerase chain reaction (PCR)과 conventional PCR을 통해 화상병균 감염 유무를 확인하였다. 그 결과, 현재까지 어떠한 미끼식물에서도 화상병균은 검출되지 않았다. 따라서, 현재 시행되고 있는 매몰 후 화상병 기주식물 3년 식재 금지 조항을 완화하는 근거로 본 연구 결과를 제시하고자 하며, 이를 통해 국내 과수산업과 농가의 피해를 최소화하는 데 기여할 수 있을 것이라 생각된다.

The fire blight caused by Erwinia amylovora (Ea) was first reported in 2015 in Korea, and the disease has rapidly spread to 22 regions until 2021. In Korea, all host plants in the apple and pear orchards where fire blight occurred should be eliminated and buried by the Plant Protection Act. To prevent the spread of the disease, all burial sites were prohibited from planting the new host plants for the next three years. To confirm the eradication efficiency of Ea and the reoccurrence of fire blight, the surveillance facilities were established on three burial sites from 2019 to 2020 in Anseong-si, Gyeonggi-do, and Chungju-si, Chungcheongbuk-do. As host plants, five apple trees of fire blight-susceptible cultivar 'Fuji', were planted in each facility. All facilities were enclosed with fences and nets and equipped with two CCTVs, motion sensors, and several other sensors for recording weather conditions to monitor the environment of the sentinel plants in real-time. The sentinel plants were checked for the reoccurrence of fire blight routinely. Suspicious plant parts were collected and analyzed for Ea detection by loop-mediated isothermal amplification polymerase chain reaction and conventional polymerase chain reaction. Until November 2022, Ea has not been detected in all sentinel plants. These results might support that the burial control of infected plants in soil works efficiently to remove Ea and support the possibility to shorten the prohibition period of host plant establishment in the burial sites.

키워드

과제정보

This work was supported by the "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ014219022022)" of the Rural Development Administration, Republic of Korea.

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