• 한국응용과학기술학회지
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• 제21권2호
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• pp.140-147
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• 2004

An aqueous solution of a commercial liquid synthetic detergent for kitchen use was photodecomposed in the presence of titanium dioxides powder under an atmosphere of air at room temperature. Titanium dioxides were prepared by sol-gel method from titanium iso-propoxide at different R ratio($H_2O$/titanium iso-propoxide) and calcined at $500^{\circ}C$. All titanium dioxides were characterized by XRD, BET surface area analyzer and UV-VIS spectrometer. The surface area of titanium dioxides prepared at R ratio=6 appeared higher volume about 20% than commercial $TiO_2$ catalysts. XRD patterns of titania particles were observed mixing phase together with rutile and anatase type. Titanium dioxides prepared by sol-gel method show higher activity about 6% than commercial $TiO_2$ catalysts on the photocatalytic degradation of a commercial liquid synthetic detergent for kitchen. The concentration of the detergent decreased to about 90% of its initial value at illumination times of 2 hour. Illumination for 30 minutes decreased the concentration of oxygen to about one-fifth of the initial value.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1749-1759
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• 2019

Aspergillus ochraceus biofilm, developed on an inert support, can produce tannase in Khanna medium containing 1.5% (w/v) tannic acid as the carbon source, at an initial pH of 5.0, for 72 h at 28℃. Addition of 0.1% (w/v) yeast extract increased enzyme production. The enzyme in the crude filtrate exhibited the highest activity at 30℃ and pH 6.0. At 50℃, the half-life (T50) was 60 min and it was 260 min at pH 6.0. In general, addition of detergents and surfactants did not affect tannase activity significantly. Tannase has potential applications in various biotechnological processes such as the production of propyl gallate and in the treatment of tannin-rich effluents. The content of tannins and total phenolic compounds in effluents from leather treatment was reduced by 56-83% and 47-64%, respectively, after 2 h of enzyme treatment. The content of tannins and total phenolic compounds in the sorghum flour treated for 120 h with tannase were reduced by 61% and 17%, respectively. Interestingly, the same A. ochraceus biofilm was able to produce tannase for three sequential fermentative process. In conclusion, fungal biofilm is an interesting alternative to produce high levels of tannase with biotechnological potential to be applied in different industrial sectors.

• 한국자기공명학회논문지
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• 제14권2호
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• pp.88-104
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• 2010

Human melanocortin-4 receptor (hMC4R) has a critical role in part of energy homeostasis, and their heterozygous mutations related in genetic cause of severe human obesity. In order to study the structure and function of these membrane proteins, it is important to prepare the samples. However, the preparation of transmembrane peptide is seriously difficult and time-consuming. Overexpression and purification of membrane proteins was reported to be difficult due to their innate insoluble and toxic properties. Among the many difficulties, the most important is the difficulty in obtaining sufficient quantities of purified protein. Recently, we succeed to produce large amounts of the second transmembrane domain from the wild-type hMC4R (wt-TM2) and D90N mutant hMC4R (m-TM2) and proposed the structural difference of them in membrane-like environments. In this paper, we demonstrate the optimization procedures to express and purify wt-TM2 or m-TM2 peptides, and solution NMR studies in different detergents to get high-resolution spectra were also described.

• 한국환경과학회지
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• 제5권5호
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• pp.579-583
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• 1996

본 연구는 날로 더해가는 오염의 직접간접 원인인 고분자성 제품류의 분해 촉진에 사용될 촉매 개발의 일차적 연구로서, 구리 촉매작용에 의한 아미드 결합의 분해 반응을 수행하였다. 가시광선 스펙트럼의 변화를 측정함으로써 반응을 추적하였다. 아미드 리간드를 포함하는 구리 화합물에서 수용액의 pH의 증가에 따라, 온도의 증가에 따라 아미드의 반응속도가 증가한다. 반응속도는 구리 화합물에 대하여 1차 반응으로 밝혀졌다. 반응의 중간체로 구리-히드록시 화합물이 관여하는 반응 메카니즘을 제시하였다. 분해 반응 메카니즘의 확실한 이해를 통하여 펩티드 결합의 분해 반응에 사용될 좋은 촉매 개발에의 응용이 기대 된다.

• 한국응용과학기술학회지
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• 제11권2호
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• pp.39-54
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• 1994

I am going to survey major topics relating to the title by analyzing the following statistical data. 1 : Global trend of production and import of oil and relating materials 2 : Global trend of major oil production 3 : Capacity of Japanese factory for oil production 4 : Situation of mechanical expression based on raw materials 5 : Change of production amounts of Oil products 6 : Oil production of each items 7 : Imported amounts of oil products 8 : Vegetable oil meals, production, import and global trend 9 : Production of mayonnaise, dressing and edible processing oils 10 : Intake of nutrients, supply of lipids, and consumption of oils 11 : Global production of oleochemicals 12 : Sales amount of oleochemicals 13 : Capacities of fatty acid and fatty alcohol factories 14 : Oleochemical production in 1995 15 : Oleochemical procuction in asia 16 : Production of surfactants for industrial use 17 : Sales amount of detergents and washing materials 18 : Production of soap and synthetic detergent in Japan 19 : Consumption of soap. detergent, and synthetic detergent 20: Relation beween synthetic detergent production and popularization 21 : Biodegradability of soft detergent

• 복식문화연구
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• 제22권3호
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• pp.503-510
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• 2014

Nowdays, costumer's washing behavior were often times for remove to light-duty dirt. The purpose of this study is to provide the most effective washing conditions of detergent concentration and temperature conditions for help save energy and water resources required to the washing process. Washing conditions were as follows: 2 kinds of detergents and 4 types of artificially soiled fabrics were used. Detergent concentration was 0%, -10%, and -20% below the recommended conc. of manufacturer's standard. Temperature of bath was $20^{\circ}C$ and $40^{\circ}C$. The results of this study were as follows: First, washing performances of all kind of artificially soiled fabric improved as the temperature of bath at $40^{\circ}C$. Second, the test was conducted under the condition of decreasing respectively -10% and -20% below of the recommended concentration of manufacturer's standard detergent. The average of washing efficiency was not considerably affected by the reduction of 10% below of manufacturer's standard. Third, in case of the water temperature were $20^{\circ}C$, polyester/cotton blended fiber were higher degree of washing efficiency than cotton fiber.

• 한국자기공명학회논문지
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• 제19권3호
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• pp.137-142
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• 2015

NMR-based structural studies on membrane proteins are appreciated quite challenging due to various reasons, generally including the narrow dispersion of NMR spectra, the severe peak broadening, and the lack of long range NOEs. In spite of the poor biophysical properties, structural studies on membrane proteins have got to go on, considering their functional importance in biological systems. In this review, we provide a simple overview of the techniques generally used in structural studies of membrane proteins by solution NMR, with experimental examples of a helical membrane protein, caveolin 3. Detergent screening is usually employed as the first step and the selection of appropriate detergent is the most important for successful approach to membrane proteins. Various tools can then be applied as specialized NMR techniques in solution that include sample deteuration, amino-acid selective isotope labeling, residual dipolar coupling, and paramagnetic relaxation enhancement.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.872-877
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• 2013

In a previous study, we isolated and reported a second species of the Saccharophagus genus, Saccharophagus sp. strain Myt-1. In the present study, an alginate lyase gene (algMytC) from the genomic DNA of Myt-1 was cloned and characterized. The DNA sequence fragment obtained contained an open reading frame of 1,032 bp that encoded a protein of 343 amino acids with an estimated molecular mass of 37.6 kDa and a pI of 6.60. The deduced protein, AlgMytC, had the conserved amino acid sequences (RTELREM, QIH, YFKAGVYNQ) of the polysaccharide lyase family 7. A BLAST homology search indicated that AlgMytC shared an amino acid sequence identity of 95.9% with alg7A of S. degradans 2-40. The cloned and purified AlgMytC protein showed optimal activity at $40^{\circ}C$, and retained more than 90% of its total activity even after treatment at $25^{\circ}C$ for 24 h. AlgMytC was very alkaliphilic with an optimal pH of 9.0, and more than 90% of its activity was retained in the pH range 8.5-10.0. Moreover, AlgMytC was stable over a wide pH range. The activity of AlgMytC was also stable in the presence of various detergents.

• 대한의용생체공학회:의공학회지
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• 제34권2호
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• pp.69-72
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• 2013

The goal of this study is to develop test method for evaluating the drug eluting properties of drug eluting stents (DES). PBS and the detergent solutions, presented by each DES manufacturer, were used for drug release of DES coated with paclitaxel, zotarolimus and everolimus. The drugs which are coating DES were not released by PBS but released by the detergent solutions, finally paclitaxel 83.38%, zotarolimus 103.85% and everolimus 115.78%. It seems that the use of the detergents is necessary in order to release the drugs because those drugs are extremely hydrophobic. In conclusion, using of detergent solutions presented by each manufacturer were suitable for evaluating the drug eluting property of drug eluting stent.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.352-359
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• 2009

Panax ginseng roots produce triterpene saponins called ginsenosides, which are high value secondary metabolites and has been used as drugs, detergents, sweeteners, and cosmetics. In the recent years plant cell, tissue and organ cultures have developed as important alternative sources for the saponin production in Panax ginseng. Adventitious roots and hairy roots have been successfully induced and cultured for the improvement of saponin contents. Genetic and metabolic engineering to regulate saponin biosynthesis in P. ginseng might be important way to improve the medicinal values of P. ginseng. Here we introduced the protocol of genetic transformation and recent progress of functional characterization of genes involved in saponin biosynthesis in P. ginseng.