• Title/Summary/Keyword: Detection potential

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Analysis of Human Serum Amyloid A-1 Concentrations Using a Lateral Flow Immunoassay with CdSe/ZnS Quantum Dots (Human Serum Amyloid A-1 단백질 농도 분석을 위한 CdSe/ZnS 양자점 기반의 Lateral Flow Immunoassay 방법 개발)

  • Fajri, Aidil;Goh, Eunseo;Lee, Sanghyuk;Lee, Hye Jin
    • Applied Chemistry for Engineering
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    • v.30 no.4
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    • pp.429-434
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    • 2019
  • A lateral flow immunoassay platform utilizing antibody functionalized water soluble CdSe/ZnS semiconductor quantum dots (QDs) was developed for the analysis of human serum amyloid A-1 (hSAA1) in a buffer solution. hSAA1 was chosen as a target protein because it is regarded as a potential biomarker associated with early diagnosis and prognosis in patients of lung cancer. The immunoassay strip on a nitrocellulose membrane was fabricated by spraying two lines composed of a test line with a monoclonal antibody against hSAA1 (10G1) (anti hSAA1) and a control line of anti-chicken IgY. While the CdSe/ZnS QDs synthesized in an organic phase were transferred to a water phase by ligand exchange using carboxylic acid modified alkane thiol. The QDs was then conjugated to monoclonal antibody against hSAA1 (14F8) [anti hSAA1 (14F8)] and used as a fluorescent detection probe. The sequential lateral flow of hSAA1 in different concentration and QDs-anti hSAA1 (14F8) complex allowed to form the surface sandwich complex of anti hSAA1 (10G1)/hSAA1/QD-anti hSAA1 (14F8), which was then analyzed using fluorescence microscope. A 100 nM concentration of hSAA1 protein can be detected by naked eyes under an optimized lateral flow buffer condition with a sensing time of 5 mins.

Detecting and Avoiding Dangerous Area for UAVs Using Public Big Data (공공 빅데이터를 이용한 UAV 위험구역검출 및 회피방법)

  • Park, Kyung Seok;Kim, Min Jun;Kim, Sung Ho
    • KIPS Transactions on Software and Data Engineering
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    • v.8 no.6
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    • pp.243-250
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    • 2019
  • Because of a moving UAV has a lot of potential/kinetic energy, if the UAV falls to the ground, it may have a lot of impact. Because this can lead to human casualities, in this paper, the population density area on the UAV flight path is defined as a dangerous area. The conventional UAV path flight was a passive form in which a UAV moved in accordance with a path preset by a user before the flight. Some UAVs include safety features such as a obstacle avoidance system during flight. Still, it is difficult to respond to changes in the real-time flight environment. Using public Big Data for UAV path flight can improve response to real-time flight environment changes by enabling detection of dangerous areas and avoidance of the areas. Therefore, in this paper, we propose a method to detect and avoid dangerous areas for UAVs by utilizing the Big Data collected in real-time. If the routh is designated according to the destination by the proposed method, the dangerous area is determined in real-time and the flight is made to the optimal bypass path. In further research, we will study ways to increase the quality satisfaction of the images acquired by flying under the avoidance flight plan.

Investigation of the Molecular Diagnostic Market in Animals (동물 분자 진단 시장의 동향)

  • Park, Chang-Eun;Park, Sung-Ha
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.1
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    • pp.26-33
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    • 2019
  • Recently, the rapid growth of the companion animal market has led to the development of animal disease diagnosis kits. Therefore, the utility of the introduction of biomarkers for the development of animal molecular diagnostics is being reevaluated. A good biomarker should be precise and reliable, distinguish between normal and diseased states, and differentiate between different diseases. Recently reported genetic markers, tumor markers (cell free DNA, circulating tumor cells, granzyme, and skin tumors), and others (brucellosis, programmed death recovery-1, symmetric dimethylarginine, periostin, and cysteinyl leukotrien) have been developed. The biomarkers are used for risk prediction or for the screening, diagnosis, and monitoring of disease progression. The most important criteria for related biomarkers are disease specificity. Many potential biomarkers have emerged from laboratory and test studies, but they have not been validated in independent or large-scale clinical studies. Candidate biomarkers evaluate disease associations, verify the effectiveness of biomarkers for early detection and disease progression, and incorporate them into humans and animals. In the future, it will be necessary to reevaluate the utility of well-structured biomarker-based research and study the development of kits that can be used in on-site tests in accordance with the trends introduced in the diagnosis of animal diseases.

Application of Geophysical Methods for Developing Saline Groundwater from an Coastal Aquifer (해안지역 염지하수 개발을 위한 물리탐사 적용 사례)

  • Lim, Sung Keun;Song, Sung-Ho;Kim, Soo Hong
    • Geophysics and Geophysical Exploration
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    • v.21 no.4
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    • pp.255-261
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    • 2018
  • The purpose of this study was to obtain a large amount of saline groundwater around coastal aquaculture farms. Thus, we have proposed a method for evaluating the potential amount of saline groundwater resources through the combined analysis of geophysical methods. Refraction seismic survey and electrical resistivity survey were conducted in the vicinity of fish farm at Hadong, Gyeongnam Province. As the result, the velocity of layer in the range of 900 ~ 2,400 m/s was found to be saltwater aquifer with high water content. Geological drilling investigation and analysis of soil samples also showed that the soil at study area was the same as the texture of sandy loam layer in agricultural radial collector wells installed by KRC (Korea Rural Community Corporation). Futhermore, the study area turned out to be quite possible to develop saline groundwater from the coastal shallow aquifer. Therefore, parallel analysis of refraction seismic surveys and electrical resistivity surveys at coastal area are expected to be very useful for the detection of the aquifer composed of sand and gravel layers with high porosity in sandy sedimentary layers along the coastal area.

Validation of Sea Surface Wind Estimated from KOMPSAT-5 Backscattering Coefficient Data (KOMPSAT-5 후방산란계수 자료로 산출된 해상풍 검증)

  • Jang, Jae-Cheol;Park, Kyung-Ae;Yang, Dochul
    • Korean Journal of Remote Sensing
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    • v.34 no.6_3
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    • pp.1383-1398
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    • 2018
  • Sea surface wind is one of the most fundamental variables for understanding diverse marine phenomena. Although scatterometers have produced global wind field data since the early 1990's, the data has been used limitedly in oceanic applications due to it slow spatial resolution, especially at coastal regions. Synthetic Aperture Radar (SAR) is capable to produce high resolution wind field data. KOMPSAT-5 is the first Korean satellite equipped with X-band SAR instrument and is able to retrieve the sea surface wind. This study presents the validation results of sea surface wind derived from the KOMPSAT-5 backscattering coefficient data for the first time. We collected 18 KOMPSAT-5 ES mode data to produce a matchup database collocated with buoy stations. In order to calculate the accurate wind speed, we preprocessed the SAR data, including land masking, speckle noise reduction, and ship detection, and converted the in-situ wind to 10-m neutral wind as reference wind data using Liu-Katsaros-Businger (LKB) model. The sea surface winds based on XMOD2 show root-mean-square errors of about $2.41-2.74m\;s^{-1}$ depending on backscattering coefficient conversion equations. In-depth analyses on the wind speed errors derived from KOMPSAT-5 backscattering coefficient data reveal the existence of diverse potential error factors such as image quality related to range ambiguity, discrete and discontinuous distribution of incidence angle, change in marine atmospheric environment, impacts on atmospheric gravity waves, ocean wave spectrum, and internal wave.

A Study on National Response Strategies of Large-scale Marine Disaster (대규모 해양재난의 국가적 대응전략에 관한 연구)

  • Lee, Choonjae
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.5
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    • pp.550-559
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    • 2019
  • The sinking of the M/V SEWOL in April 2014 was not a mere marine accident, but a marine catastrophe. This grim case developed into a social tragedy that impinged the national sentiment and communal integrity. It is imperative that thorough provisions and measures be outlined at the national level with regard to massive marine accidents, oil pollution, and natural disasters that might critically affect government affairs. Pivoting on "The Black Swan Theory," a concept of improperly rationalizing a national crisis based on uncertainties, this research assesses a variety of response strategies that minimize the national economic and social damage caused by a large-scale marine disaster. Along with the effort of minimizing any potential defects in each protective barrier, the "Black Swan Detection System of the Marine Disaster" needs to be incorporated to prevent cases wherein such defects lead to an actual crisis. Maritime safety must be systematically unified under a supervisory organization, and a structure for maritime crisis on-scene command and cooperation must likewise be established in order that every force on the scene of a marine disaster may act effectively and consistently under the direction of an on-scene commander.

Development of Real-time Video Search System Using the Intelligent Object Recognition Technology (지능형 객체 인식 기술을 이용한 실시간 동영상 검색시스템)

  • Chang, Jae-Young;Kang, Chan-Hyeok;Yoon, Jae-Min;Cho, Jae-Won;Jung, Ji-Sung;Chun, Jonghoon
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.6
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    • pp.85-91
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    • 2020
  • Recently, video-taping equipment such as CCTV have been seeing more use for crime prevention and general safety concerns. Since these video-taping equipment operates all throughout the day, the need for security personnel is lessened, and naturally costs incurred from managing such manpower should also decrease. However, technology currently used predominantly lacks self-sufficiency when given the task of searching for a specific object in the recorded video such as a person, and has to be done manually; current security-based video equipment is insufficient in an environment where real-time information retrieval is required. In this paper, we propose a technology that uses the latest deep-learning technology and OpenCV library to quickly search for a specific person in a video; the search is based on the clothing information that is inputted by the user and transmits the result in real time. We implemented our system to automatically recognize specific human objects in real time by using the YOLO library, whilst deep learning technology is used to classify human clothes into top/bottom clothes. Colors are also detected through the OpenCV library which are then all combined to identify the requested object. The system presented in this paper not only accurately and quickly recognizes a person object with a specific clothing, but also has a potential extensibility that can be used for other types of object recognition in a video surveillance system for various purposes.

A Study on Detection Technique of Anomaly Signal for Financial Loan Fraud Based on Social Network Analysis (소셜 네트워크 분석 기반의 금융회사 불법대출 이상징후 탐지기법에 관한 연구)

  • Wi, Choong-Ki;Kim, Hyoung-Joong;Lee, Sang-Jin
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.22 no.4
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    • pp.851-868
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    • 2012
  • After the financial crisis in 2008, the financial market still seems to be unstable with expanding the insolvency of the financial companies' real estate project financing loan in the aftermath of the lasted real estate recession. Especially after the illegal actions of people's financial institutions disclosed, while increased the anxiety of economic subjects about financial markets and weighted in the confusion of financial markets, the potential risk for the overall national economy is increasing. Thus as economic recession prolongs, the people's financial institutions having a weak profit structure and financing ability commit illegal acts in a variety of ways in order to conceal insolvent assets. Especially it is hard to find the loans of shareholder and the same borrower sharing credit risk in advance because most of them usually use a third-party's name bank account. Therefore, in order to effectively detect the fraud under other's name, it is necessary to analyze by clustering the borrowers high-related to a particular borrower through an analysis of association between the whole borrowers. In this paper, we introduce Analysis Techniques for detecting financial loan frauds in advance through an analysis of association between the whole borrowers by extending SNA(social network analysis) which is being studied by focused on sociology recently to the forensic accounting field of the financial frauds. Also this technique introduced in this pager will be very useful to regulatory authorities or law enforcement agencies at the field inspection or investigation.

Detection of blaKPC and blaNDM Genes from Gram-Negative Rod Bacteria Isolated from a General Hospital in Gyeongnam (경남지역 종합병원에서 분리된 그람음성막대균으로부터 blaKPC 및 blaNDM 유전자 검출)

  • Yang, Byoung Seon;Park, Ji Ae
    • Korean Journal of Clinical Laboratory Science
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    • v.53 no.1
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    • pp.49-59
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    • 2021
  • This study investigated the use of real-time PCR melting curves for the diagnosis of blaKPC and blaNDM genes among the most frequently detected carbapenemase-producing Enterobacteriaceae in Korea. As a means of addressing the shortcomings of phenotype tests and conventional PCR. The modified Hodge test confirmed positivity in 25 of 35 strains, and carbapenemase inhibition testing confirmed positivity in 14 strains by meropenem+PBA or meropenem+EDTA. PCR analysis showed amplification products in 25 strains of Klebsiella pneumoniae carbapenemases (KPC), 10 of K. pneumoniae, 5 of E. coli, 5 of A. baumannii, 4 of P. aeruginosa, and 1 of P. putida. New Delhi metallo β-lactamase (NDM) identified amplification products in 8 strains, that is, 2 K. pneumoniae, 3 E. coli, 1 P. aeruginosa, 1 E. cloacae, and 1 P. retgeri strains. Real-time PCR melting curve analysis confirmed amplification in 25 strains of KPC and 8 strains of NDM, and these results were 100% consistent with PCR results. In conclusion, our findings suggest early diagnosis of carbapenem resistant Enterobacteriaceae by real-time PCR offers a potential means of antibacterial management that can prevent and control nosocomial infection spread.

Immobilization of As and Pb in Contaminated Soil Using Bead Type Amendment Prepared by Iron NanoparticlesImpregnated Biochar (철 나노 입자가 담지된 바이오차 기반 비드 형태 안정화제를 이용한 비소 및 납 오염토양의 안정화)

  • Choi, Yu-Lim;Kim, Dong-Su;Kang, Tae-Jun;Yang, Jae-Kyu;Chang, Yoon-Young
    • Journal of Environmental Impact Assessment
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    • v.30 no.4
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    • pp.247-257
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    • 2021
  • In this work, Iron Nano-Particles Impregnated BioChar/bead (INPBC/bead) soil amendment was developed to increase biochar's reactivity to As in soil and preventing possible wind loss. Prior to preparation of INPBC/bead, INPBC was produced utilizing lignocellulosic biomass and Fe(III) solution in a hydrothermal method, followed by a calcination process. Then, the bead type amendment, INPBC/bead was produced by cross-linking reaction of alginate with INPBC. FT-IR, XRD, BET, and SEM-EDS analyses were utilized to characterize the as-synthesised materials. The particle size range of INPBC/bead was 1-4 mm, and different oxygen-containing functional groups and Fe3O4 crystalline phase were produced on the surface of INPBC/bead, according to the characterization results. The soil cultivation test was carried out in order to assess the stabilization performance of INPBC/bead utilizing As and Pb-contaminated soil obtained from an abandoned mining location in South Korea. After 4 weeks of culture, TCLP and SPLP extraction tests were performed to assess the stabilization efficacy of the amendment. The TCLP and SPLP findings revealed that raising the application ratio improved stabilizing efficiency. The As stabilization efficiency was determined to be 81.56 % based on SPLP test findings for a 5% in (w/w) INPBC/bead treatment, and the content of Pb in extracts was reduced to the limit of detection. According to the findings of this study, INPBC/bead that can maintain pH of origin soil and minimize wind loss might be a potential amendment for soil polluted with As and heavy metals.