• Laboraroty Animal Research
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• v.34 no.4
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• pp.302-310
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• 2018

CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein ${\alpha}$ ($SIRP{\alpha}$) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout ($CD47^{-/-}$ hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, $CD47^{-/-}$ mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks. Hematological analysis revealed a mild but significant reduction of RBC counts and hemoglobin in 16 week-old male $CD47^{-/-}$ mice which were aggravated at the age of 26 weeks with increased reticulocyte counts and mean corpuscular volume (MCV), suggesting hemolytic anemia. Interestingly, anemia in female $CD47^{-/-}$ mice became evident at 26 weeks, but splenomegaly was identified in both genders of $CD47^{-/-}$ mice from the age of 16 weeks, consistent with development of hemolytic anemia. Additionally, helper and cytotoxic T cell populations were considerably reduced in the spleen, but not in thymus, of $CD47^{-/-}$ mice, suggesting a crucial role of CD47 in proliferation of T cells. Collectively, these findings indicate that our $CD47^{-/-}$ mice have progressive hemolytic anemia and splenic depletion of mature T cell populations and therefore may be useful as an in vivo model to study the function of CD47.

• Environmental and Resource Economics Review
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• v.28 no.2
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• pp.277-306
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• 2019

Mackerel is one of the most widely consumed aquatic products in Korea. Concerns about the depletion of stocks have also arisen as the catch has decreased. The primary purpose of this study is to estimate the mackerel stock and derive the optimal level of catch in Korea. We apply a generalized maximum entropy econometric method to estimate the mackerel growth function, which does not require the steady state assumption. We incorporate a bootstrapping approach to derive the significance levels of parameter estimates. We found that the average ratio of catch to the estimated total stock was less than 30% before the 1990s but exceeded 40% in the 1990s. After 2000, it dropped back to about 36%. This finding indicates that mackerel may have been over-fished in the 1990s, but the government regulations introduced in the 2000s alleviated over-fishing problems. Nevertheless, our dynamic optimization analysis suggests that the total allowable catch may need to be carefully controlled to achieve socially optimal management of resources.

• Convergence Security Journal
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• v.17 no.5
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• pp.63-69
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• 2017

In the early 1990s, IETF(Internet Engineering TaskForce) had started the discussion on new address protocol that can modify and supplement various drawbacks of existing IPv4 address protocol with the introduction of CIDR(Classless Inter-Domain Routing) which is a temporary solution for IPv4 address depletion, NAT, private IP address. While various standards related to new address protocol has been proposed, the SIPP(Simple Internet Protocol Plus) was adopted among them because it is regarded as the most promising solution. And this protocol has been developed into current IPv6. The new concepts are introduced with modifying a lot of deficiencies in the exisitng IPv4 such as real-time data processing, performance on QoS, security and the efficiency of routing. Since many security threats in IPv6 environment still exist, the necessity of stable data communication environment has been brought up continuously. This paper deveopled one-way communication algorithm in IPv6 based on the high possibility of protecting the system from uncertain and potential risk factors if the data is transmitted in one way. After the analysis of existing IPv6 and ICMPv6, this paper suggests one-way communication algorithm as a solution for existing IPv6 and ICMPv6 environment.

• Applied Chemistry for Engineering
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• v.32 no.3
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• pp.332-339
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• 2021

Recently, due to concerns about the depletion of fossil fuels and the emission of greenhouse gases, the importance of hydrogen energy technology, which is a clean energy source that does not emit greenhouse gases, is being emphasized. Water electrolysis technology is a green hydrogen technology that obtains hydrogen by electrolyzing water and is attracting attention as one of the ultimate clean future energy resources. In this study, the surface properties of the porous transport layer (PTL), one of the cell components of the proton exchange membrane water electrolysis (PEMWE), were controlled using a sandpaper to reduce overvoltage and increase performance and stability. The surfaces of PTL were sanded using sandpapers of 400, 180, and 100 grit, and then all samples were finally treated with the sandpaper of 1000 grit. The prepared PTL was analyzed for the degree of hydrophilicity by measuring the water contact angle, and the surface shape was observed through SEM analysis. In order to analyze the electrochemical characteristics, I-V performance curves and impedance measurements were conducted.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.13 no.6
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• pp.556-561
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• 2020

The aging and declining agricultural population in the modern society requires improvement of the agricultural environment and is one of the representative problems. And since most of the work systems always require a transport work, the ratio of labor consumed in the transport work is very high. Accordingly, many types of transport vehicles are being developed and sold, and in the early days, most of them are powered transport vehicles using fossil fuels. However, it is paying attention to next-generation eco-friendly energy such as hydrogen, fuel cells, solar power, and bio due to the strengthening of international environmental regulations such as global warming and the Convention on Climate Change and the depletion of fossil fuels. Therefore, in this study, the ultimate goal is to develop an eco-friendly, easy-to-operate, safe agricultural electric vehicle that replaces fossil fuels. It was designed with a focus on controlling a wide range of vehicle speeds and securing stability of electric agricultural vehicles. Considering the performance and design, it is composed of a frame, a driving part, a steering part, and a controller system, and we are going to review and manufacture each part. It is believed that the manufactured electric vehicle for agriculture can be easily and conveniently operated in an agricultural society where young manpower is scarce, and can be helpful to the agricultural society through high efficiency.

• The Journal of the Korea Contents Association
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• v.22 no.6
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• pp.629-637
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• 2022

It is at risk of depletion of biodiversity due to indiscriminate overfishing of ecosystems and destruction of habitats. Intensive fertilizers or development of related facilities to increase agricultural production in poor indigenous areas devastate the soil. Preservation of biodiversity is now emerging as an important issue of global human coexistence. After the Post-2020 GBF Declaration, all governance in agricultural development in indigenous agricultural areas should be supported and promoted as biodiversity conservation measures. A compromise plan to reduce ecosystem development and biodiversity loss can help establish public governance policies. In this paper, a viability kernel used for viable control feedback analysis is introduced to solve conflicting economic and ecological problems in ecosystem conservation, and a mathematical model on biodiversity conservation by the viability kernel is examined. Because all species in the ecosystem are interdependent, if the balance is broken, biodiversity is depleted, which is irreversible and eventually leads to extinction. For sustainable use and harmony of biological resources, a lot of policy consideration is required, such as creative governance that can efficiently protect all species. Subsidies or tax incentives have a direct impact on biodiversity conservation. The recovery of species in a state of decreasing biodiversity can be said to be of great economic value. Biodiversity will allow indigenous producers to be proud of their unique traditional knowledge and have a positive impact on local tourism, thereby enhancing regional identity and greatly contributing to the survival and prosperity of mankind.

• Journal of Korean Tunnelling and Underground Space Association
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• v.23 no.6
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• pp.403-421
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• 2021

Most of the urban tunnels in Korea, which are represented by the 1st to 3rd subways, use the drainage tunnel by NATM. Recently, when a construction project that actively utilizes large-scale urban space is promoted, negative effects that do not conform to the existing empirical rules of urban tunnels may occur. In particular, there is a high possibility that groundwater fluctuations and hydrodynamic behavior will occur owing to the practice of tunnel technology in Korea, which has mainly applied the drainage tunnel. In order to solve the problem of the drainage tunnel, attempts are being made to control groundwater fluctuations. For this, the establishment of tunnel groundwater management standard concept and the analysis of the tunnel hydraulic behavior were performed. To prevent the problem of groundwater fluctuations caused by the construction of large-scale tunnels in urban areas, it was suggested that the conceptual transformation of the empirical technical practice, which is applied only in the underground safety impact assessment stage, to the direction of controlling the inflow in the tunnel, is required. And the relationship between the groundwater level and the inflow of the tunnel required for setting the allowable inflow when planning the tunnel was derived. The introduction of a tunnel groundwater management concept is expected to help solve problems such as groundwater fluctuations, ground settlement, depletion of groundwater resources, and decline of maintenance performance in various urban deep tunnel construction projects to be promoted in the future.

• KIPS Transactions on Software and Data Engineering
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• v.11 no.8
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• pp.339-346
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• 2022

Recently, the resource depletion and climate change problem caused by the massive usage of fossil fuels for electric power generation has become a critical issue worldwide. According to this issue, interest in renewable energy resources that can replace fossil fuels is increasing. Especially, photovoltaic power has gaining much attention because there is no risk of resource exhaustion compared to other energy resources and there are low restrictions on installation of photovoltaic system. In order to use the power generated by the photovoltaic system efficiently, a more accurate photovoltaic power forecasting model is required. So far, even though many machine learning and deep learning-based photovoltaic power forecasting models have been proposed, they showed limited success in terms of interpretability. Deep learning-based forecasting models have the disadvantage of being difficult to explain how the forecasting results are derived. To solve this problem, many studies are being conducted on explainable artificial intelligence technique. The reliability of the model can be secured if it is possible to interpret how the model derives the results. Also, the model can be improved to increase the forecasting accuracy based on the analysis results. Therefore, in this paper, we propose an explainable photovoltaic power forecasting scheme based on BiLSTM (Bidirectional Long Short-Term Memory) and SHAP (SHapley Additive exPlanations).

• Proceedings of the Botanical Society of Korea Conference
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• 1994.09a
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• pp.29-39
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• 1994

Serious issues about the changes in the environmental conditions on earth associated with human activities have arisen, and the interest in these problems has increased. It is urgent to determine how the expansion of terrestrial UV-B radiation due to the stratospheric ozone depletion influences living matters. In this connection, we have been investigating the effects of UV-B radiation on the growth of rice cultivars (Oryza sativa L.). We report here some physiological and genetic aspects of resistance to inhibitory effects of UV-B radiation on growth of rice cultivars as described below. Elevated UV radiation containing large amount of UV-B and a small amount of UV-C inhibited the development of plant height, the photosynthetic rate and the chlorophyll content in rice plants in a phytotron. Similar results were obtained in experiments, in which elevated UV-V radiation. Similar results were obtained in experiments, in which elevated UV-B radiation (transmission down to 290 nm) was applied instead of UV-B radiation containing a small amount of UV-C. The inhibitory effects of UV radiation was alleviated by the elevated CO2 atmospheric environment or by the exposure to the high irradiance visible radiation. The latter suggested the possibility that the resistance to the effects of UV radiation was either due to a lower sensitivity to UV radiation or to a greater ability to recover from the injury caused by UV radiation through the exposure to visible radiation. The examination of cultivar differences in the resistance to UV radiation-caused injuries among 198 rice cultivars belonging to 5 Asian rice ecotypes (aus, aman, boro, bulu and tjeleh) from the Bengal region and Indonesia and to Japanese lowland and upland rice groups showed the following: Various cultivars having different sensitivities to the effects of UV radiation were involved in the same ecotype and the same group, and that the Japanese lowland rice group and the boro ecotype were more resistant. Among Japanese lowland rice cultivars, Sasanishiki (one of the leading varieties in Japan) exhibited more resistance to UV rakiation, while Norin 1 showed less resistance, although these two cultivars are closely related. It was thus indicated that the resistance to the inhibitory effects of UV radiation of rice cultivars is not simply due to the difference in the geographical situation where rice cultuvars are cultivated. Form the genetic analysis of resistance to the inhibitory effects of UV radiation on growth of rice using F2 plants generated by reciprocally crossing Sasanishiki and Norin 1 and F3 lines generated by self-fertilizing F2 plants, it was evident that the resistance to the inhibitory of elebated UV radiation in these rice plants was controlled by recessive polygenes.

• Journal of Life Science
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• v.33 no.1
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• pp.8-14
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• 2023

Peroxidasin (PXDN), a multidomain heme peroxidase containing extracellular matrix (ECM) motifs, as well as a catalytic domain, catalyzes the sulfilimine crosslink of collagen IV (Col IV) to reinforce Col IV scaffolds. We previously reported that PXDN is required for endothelial cell (EC) survival and growth signaling through sulfilimine crosslink-dependent matrix assembly. In this study, we examined whether peroxidase activity is required for PXDN function in ECs. First, we constructed a mutant PXDN by point mutation of two highly conserved amino acids, Q823 and D826, which are present in the active site of the peroxidase domain. After isolation of HEK293 clones highly expressing the mutant protein, conditioned medium (CM) was obtained after incubating the cells in serum-free medium for 24 hours and then analyzed by Western blot analysis under nonreducing conditions. The results revealed that the mutant PXDN formed a trimer and that it was cleaved by proprotein convertase-like wild-type (WT) PXDN. However, peroxidase activity was not detected in the CM containing the mutant PXDN, in contrast to that of WT PXDN. In addition, the sulfilimine crosslink ability of the mutant PXDN was lost. Moreover, the CM containing the mutant PXDN failed to promote the growth of PXDN-depleted ECs, unlike the CM containing WT PXDN. These results suggest that the peroxidase activity of PXDN affects EC growth by forming a sulfilimine crosslink.