• Title/Summary/Keyword: Dentocult-SM

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The correlation between dental caries experience and improved dental caries activity tests for the students of dental hygiene (치위생과 학생의 치아우식경험도와 개량형 우식활성검사와의 관련성)

  • Cho, Min-Jung;Kim, Jin-Hee;Kim, Eun-Mi;Lee, Hyang-Nim
    • Journal of Korean society of Dental Hygiene
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    • v.3 no.2
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    • pp.197-208
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    • 2003
  • This study was implemented for 84 students of dental hygiene to show the correlation between dental caries experience and improved caries activity test. Dental caries experience for the sample groups was examined and stimulative saliva secreted for 5 minutes was collected into the tube to check saliva secretion rate. Dentocult LB test was executed to observe Lactobacilli colonies after 96 hour cultivation of culture slides moistened with stimulative saliva. Dentocult SM test(screening strip, site strip) was done to measure SM colonies distribution after 48 hour cultivation of culture strips applied with collected saliva and dental plaque respectively, and salivary buffering capacity was checked by means of Dentobuff strip kit. Following conclusions are obtained after examining the relation between Dentocult LB, Dentocult SM, Dentobuff strip test results and DMFT index, salivary secretion rate. 1. Showed no significant difference between Dentocult LB test results and DMFT index, salivary secretion rate. 2. Showed no significant difference between Dentocult SM(screening strip) test results and DMFT index, salivary secretion rate. 3. Showed significant difference between Dentocult SM(site strip) test results and DMFT index(pE0.05), but showed no significant difference between Dentocult SM(site strip) test results and salivary secretion rate. 4. Showed no significant difference between Dentobuff strip test results and DMFT index, but showed a very wide difference between Dentobuff strip test results and salivary secretion rate(pE0.01).

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The study of caries activity test by multiplex-quantity real time PCR with easy perio test (구강세균 유전자 검사(easy perio test)법을 이용한 치아우식 검사)

  • Yun, Han Gyeol;Park, Seong Gyu;Kim, Jin
    • Journal of Korean Academy of Dental Administration
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    • v.6 no.1
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    • pp.11-18
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    • 2018
  • The aim of this study was to evaluate the competency of the Easyperio test, a genetic test method based on real time PCR for the detection of bacteria that cause dental caries and periodontal disease. To verify the validity of this text, various dental health evaluations were administered to 33 boys between the ages of 12 to 14, as this age group commonly experiences dental caries. These evaluations included a dental caries experience survey, a first molar health evaluation, the Dentocult Streptococcus mutans (SM) strip mutans, the Dentocult Lactobacillus spp (LB) test, and the Easyperio test. The correlation coefficients between the level of the Dentocult SM strip mutans and the dental caries experience were DT (R=0.570, p=0.001), DMFT (R=0.376, p=0.031), and first molar health (R=-0.395, p=0.023). The correlation coefficients between the amount of SM in the Easyperio test and dental caries experience were DT (R=0.528, p=0.002), DMFT (R=0.369, p=0.035), and first molar health (R=-0.426, p=0.013). The correlation coefficients between the level of the dentocult SM strip mutans and the SM amounts of the Easyperio test were S.mi (R=0.564 p=0.001) and S.mu (R=0.621, p=0.002). The correlation coefficients between the level of the Dentocult LB test and the SM amount of Easyperio test was S.mi (R=0.495, p=0.003). In conclusion, Easyperio test may be an easy and effective method for the differentiation and diagnosis of dental caries through quantitative and qualitative analysis of oral bacteria.

Correlation between Caries Experience and New Colorimetric Caries Activity Test in Children (소아에서 치아 우식 경험과 새로운 치아 우식 활성 비색 검사)

  • Cho, Seonghyeon;Lee, Hyoseol;Choi, Byungjai;Kim, Bakil;Kim, Seongoh;Choi, Hyungjun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.1
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    • pp.30-37
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    • 2015
  • A new colorimetric test ($Cariview^{(R)}$) using a new type of pH indicator can reflect the acidogenic potential of plaque bacteria. The objective of this study was to evaluate the correlation between $Cariview^{(R)}$ and the caries experience (measured through the dmft index) of children, and to compare $Cariview^{(R)}$ with Dentocult $SM^{(R)}$. Having obtained informed consent, 135 children less than 6 years old participated in the study. We examined their dmft index, and performed two caries activity tests ($Cariview^{(R)}$ and Dentocult $SM^{(R)}$) according to the manufacturers' instructions. In the results, $Cariview^{(R)}$ showed a moderate correlation with the dmft index (r = 0.43, p < 0.01). $Cariview^{(R)}$ showed a sensitivity of 68.8%, a specificity of 69.2%, and an area under curve of 0.686 in the ROC curve analysis. $Cariview^{(R)}$ had a significant correlation with the children's caries experience and had a slightly better explanatory ability than Dentocult $SM^{(R)}$. Furthermore, $Cariview^{(R)}$ was convenient and easy to use on uncooperative children, and also had an educational effect with its visual colors. It is suggested that $Cariview^{(R)}$ could be used clinically to identify the children susceptible to develop caries and to establish a preventive strategy.

RELATIONSHIP BETWEEN DENTOCULT-SM TEST, MICROBIAL ANALYSIS AND DENTAL CARIES IN THE PRE-SCHOOL CHILDREN (미취학아동들의 유치우식경험도와 개량형 Dentocult-SM 검사 및 치면세균막 세균활성과의 관련성)

  • Shin, Doo-Kyo;Kim, Ji-Young;Song, Keun-Bae;Nam, Soon-Heyun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.2
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    • pp.254-262
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    • 2003
  • Dental caries has a multifactorial aetiology in which there is an interplay of three principal factors: the host(saliva and teeth), the microflora(dental plaque) and the substrate(diet), and a fourth factor time. There is no single test that takes into consideration all these factors and can accurately predict an individual's susceptibility to dental caries. The risk of dental caries can be evaluated by analysing and integrating several causative factors. The objective of the study was to clarify the relationship between caries activity and the status of caries of primary teeth and the relationship between the quantity of S. mutans in the dental plaque and the status of dental caries. Forty nine children ages 5 were examined with caries indices, Dentocult-SM and quantity of S. mutans. The results were as follows: All the survey results were similar with national means of 5-6 years Korean preschool children. The deft index of subjects was 5.46 and defs index was 9.81. There was 8.6% of negative, 10.9% of mild, 28.1% of moderate and 52.3% of active results at screening strip test. 8.6% was negative, 17.2% was mild, 33.6% was moderate and 40.6% was active in site strip test. The Spearman correlation coefficient was 0.775, and overall kappa index was 0.496 between screening strip test and site strip test. There also was a high correlation between caries incidence, Dentocult-SM test, and quantity of S. mutans. Dentocult-SM test as a caries activity test is a reliable method for measuring the status of dental caries in primary teeth.

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A study on analysis of the Relationship Oral bacteria to dental caries experience in disabled student's: in Gyeong-nam (경남지역 특수학교 학생의 구강내 세균검사에 따른 영구치우식경험률에 관한 연구)

  • Cho, Pyeong-Kyu;Park, Sung-Suk
    • Journal of Korean society of Dental Hygiene
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    • v.8 no.4
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    • pp.115-125
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    • 2008
  • The purpose of this study was to examine the relationship between the Dentocult SM and LB scores of students and their DMF rate. The subjects in this study were 134 special school students in south Gyeong-sang province. After they were tested to find out their, they received tooth brushing education and preventive treatment. The test, education and treatment were all conducted from March 17 to April 9, 2008. Their oral state was checked and recorded by using a mirror and explorer, and then the data on oral state were analyzed to their DMFT, DT, FT and MT rates. All the collected data were analyzed with SPSS 15.0 for Windows program, and independent-samples t-test, one-way ANOVA and crosstabs analysis were implemented to see what differences their disability type, gender, Dentocult-SM scores and Dentocult-LB scores made. The findings of the study were as follows: 1. As for connections between disability type and DMFT rate, the students with mental retardation were similar to the other students in that regard. The mentally retarded students had higher DT and FT rates than the other students, and the MT rate of the latter was higher than that of the former. 2. Concerning the relationship of gender to DMFT and DT rates, the girls had larger DMFT and DT rates than the boys, and the FT and MT rates of the boys were higher than those of the girls. 3. As to links between Dentocult SM scores on the tongue and DMFT, the students whose bacteria was mildly activated had the highest DMFT rate, and the students who had a severely activated bacteria had the highest DT and FT rates. Those who had a moderately activated bacteria had the highest MT rate. 4.Regarding the connection of Dentocult SM scores on maxillyright molar to DMFT and DT rates, the students whose bacteria was severely activated had the highest DMFT and DT rates. Those who had no activated bacteria had the largest FT rate, and the students who had a mildly activated bacteria had the highest MT rate. 5.Regarding the connection of Dentocult SM scores on maxillyleft molar to DMFT and DT rates, the students whose bacteria was severely activated had the highest DMFT and DT rates. Those who had no activated bacteria had the largest FT rate, and the students who had a mildly activated bacteria had the largest MT rate. 6. Regarding the connection of Dentocult SM scores on mandibularright molar to DMFT and DT, FT rates, the students whose bacteria was severely activated had the highest DMFT and DT, FT rates. Those who had a moderately activated bacteria had the highest MT rate. 7. Regarding the connection of Dentocult SM scores on mandibularleft molar to DMFT and DT rates, the students whose bacteria was mildly activated had the highest DMFT and DT rates. Those who had no activated bacteria had the largest FT rate, and the students who had a moderately activated bacteria had the highest MT rate. 8. Regarding the connection of Dentocult LB scores to DMFT and DT rates, the students whose bacteria was severely activated had the highest DMFT and DT rates. Those who had no activated bacteria had a moderately FT rate, the students who had a mildly activated bacteria had a mildly MT rate.

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A study on the incremental oral health care of C pediatric clinic using a Dentocult-SM test (C소아치과의원의 개량형 Dentocult-SM검사를 이용한 계속관리에 관한 조사 연구)

  • Woo, Hee-Sun
    • Journal of Korean society of Dental Hygiene
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    • v.8 no.2
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    • pp.39-51
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    • 2008
  • The research was conducted to 100 child patients selected by random sampling, which got a Dentocult-SM test in the first visit and then was being continuously managed, out of child patients of a pediatric clinic located in Gyeonggi-do. The period of there search is one year from June 2007 to May 2008, Using Dentocult-SM test, we analyzed the correlation between the distribution of dental plaque, a streptococcus mutans in saliva and condition of dental caries cavity in the teeth of child patients, then we measured the distribution of a streptococcusmutans. According to SM score, we applied incremental oral heath care for child patients to clinical and obtained the following results, 1. In terms of the age of child patients in research, the number of 3 years old patients was 29(lst ranked), the number of 2 years old patients was 28(2nd ranked). 2. The result of SM score showed that female child patients(52.0%) was higher than male ones in negative, male child patients(52.0%) was higher than female ones in mild, female child patients(68.2%) was higher than male ones in moderate, male child patients(57.1%) was higher than female ones in severe. 3. At the first visit, the SM score showed statistically remarkable difference between dt and dmft. We can also confirm the average of severe is the highest. 4. At the second visit, the SM score showed statistically remarkable difference among dt, ft, and dmft index We can also confirm the average of severe is the highest. 5. At the third visit, The SM score showed statistically remarkable difference among dt, ft, and dmft index We can also confirm the average of severe is the highest. 6. The comparison of dmft index differences to SM score showed statistically no remarkable difference in incremental oral heath care for negative and mild, In addition to that, we can confirm that the incremental oral heath care makes statistically remarkable differences in moderate and severe. 7. The comparison of dt index differences to SM score showed statistically no remarkable difference in incremental oral heath care for negative, mild, and moderate, In addition to that, we can con firm that the incremental oral heath care makes statistically remarkable differences in severe. 8. The comparison of mt index differences to SM score showed statistically no remarkable difference in incremental oral heath care for mild and moderate, In addition to that, we can confirm that the incremental oral heath care makes statistically remarkable differences m severe. 9. The comparison of ft index differences to SM score showed statistically no remarkable difference in incremental oral heath care for mild, In addition to that, we can confirm that the incremental oral heath care makes statistically remarkable differences in negative, moderate, and severe. 10. According to the comparison of dmft index to the age, the 4 years old patients showed the highest number(5.50 in the first visit and 6,08 in the second one). In the third visit, the 6 years old patients showed the highest number(7.00). By the above results, we can find that the incremental oral heath care by SM score makes the results of oral care better. Therefore, the improvement or maintenance in oral health of child patients needs continuing personal oral health management and regular systematic management focused on prevention by the specialist.

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Detection of Streptococcus mutans in Saliva using Monoclonal Antibodies (단일클론항체를 사용한 타액 내 Streptococcus mutans 검출)

  • Lee, Minjeong;Lee, Daewoo;Kim, Miah;Lee, Kyungyeol;Baik, Byeongju;Yang, Yeonmi;Kim, Jaegon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.1
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    • pp.10-21
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    • 2015
  • The purpose of this study was to determine the usefulness of a detection method for Streptococcus mutans in saliva with monoclonal antibodies developed targeting Ag I/II and glucosyltransferases (gtf B, gtf C and gtf D) in Streptococcus mutans. In the three groups tested (adults, minors, and minors under orthodontic treatment), the results of the DMFT scores, the colony density (CFU/mL) in their saliva was measured using $Dentocult^{(R)}$-SM strip mutans, polymerase chain reaction was performed to test whether Streptococcus mutans and Streptococcus sobrinus were present, and Streptococcus mutans detecting tests performed in their saliva using four types of monoclonal antibody were collected. In conclusion, it was demonstrated that the Streptococcus mutants plays more important role in forming dental caries compared to Streptococcus sobrinus, and that the monoclonal antibodies against glucosyltransferases (gtf B, gtf C, gtf D) and Ag I/II of Streptococcus mutans are superior in detecting Streptococcus mutans to $Dentocult^{(R)}$-SM strip mutans or polymerase chain reaction.

Antimicrobial Effect of Mouthwash against Streptococcus mutans by Visual Staining Method (시각적 염색 방법을 이용한 마우스워시의 구강균에 대한 항균효과 확인)

  • Park, Taehun;Cho, Jeong Hun;Sung, Youngeun;Cho, Jun-Cheol;Shin, Kyeho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.2
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    • pp.187-193
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    • 2014
  • Dental caries are one of the most common oral diseases and Streptococcus mutans (S. mutans) plays an important role in the initiation and progression of dental caries. Oral malodor is primarily the result of microbial metabolism such as Porphyromonas gingivalis (P. gingivalis) that produce volatile sulfur compounds (VSCs), causing oral malodor. Prevotella intermedia (P. intermedia) is known as typical periodontopathic bacteria, and periodontal disease is chronic inflammatory disease that leads to damage of gingival connective tissue and alveolar bone, eventually loss of teeth. In this study, we investigated antimicrobial effect of mouthwash containing cetylpyridinium chloride (CPC), sodium fluoride (NaF), green tea water extract and pine needles water extract against cariogenic and periodontopathic bacteria sucn as S. mutans, P. gingivalis and P. intermedia. As a result, the reduction ratios of S. mutans and P. gingivalis were 4.00 Log and 4.68 Log reduction for 30 s, and P. intermedia were 2.40 Log reduction for 30 s and 2.70 Log reduction for 60 s. Dentocult SM Strip mutans (SM Strip) provides easy detection of visual data showing a significant inhibition on S. mutans. In conclusion, we expected that mouthwash containing CPC, NaF, green tea water extract and pine needles water extract could help preventing the dental disease like dental caries and oral malodor.

THREE-YEAR LONGITUDINAL STUDY ON THE PREDICTABILITY OF THE RELATED FACTORS OF THE CARIES INCIDENCE ACCORDING TO THE DURATION (예측기간에 따른 영구치 우식발생 연관 요인의 예측능 평가)

  • Kim, Sung-Ki;Kim, Jin-Bom;Bae, Kwang-Hak;Kim, Shin;Jeong, Tae-Sung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.4
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    • pp.395-402
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    • 2010
  • The purpose of this study was to analyse the stability and validity of the related factors to the caries incidence according to the duration. The subjects were 249 elementary school students. Among them, the number of male students was 137 (55.0%). In the first year, all subjects gave the responses of the questionnaire composed of demographic variables and oral heath behaviors. They also received oral examination and tests of Dentocult SM and Dentocult LB. In the 2nd-4th year, they received second oral examination for the assessment of caries incidence on permanent teeth. The relation of oral health-related factors with caries incidence was analyzed by chi square method and adjusted Relative Risk (RR). In the caries incidence rate for 1 year, those who had 2 or higher score of dentocult LB was 2.3 times higher than those who had 1 or lower. The caries incidence rate for 2 years was highly associated with caries on deciduous molars and dentocult LB. The caries on deciduous molars showed strong association with the caries incidence for 3 years. It was suggested that the association between the caries incidence and the related factors was different according to the duration. Therefore, dentists could need to consider the visiting period in the education of the risk factors of dental caries.

DETECTION SYSTEM OF STREPTOCOCCUS MUTANS IN SALIVA USING MONOCLONAL ANTIBODY (Monoclonal Antibody를 이용한 Streptococcus mutans 검출 방법의 임상적 적용에 관한 연구)

  • Hong, Hi-Jung;Kim, Jong-Soo;Kim, Yong-Kee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.36 no.4
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    • pp.522-530
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    • 2009
  • The purpose of this study was to evaluate the clinical effectiveness of new streptococcus detection system which used monoclonal antibody against Streptococcus mutans. 92 children aged between 2 and 8 were involved in this experiment and their saliva samples were collected for testing. Streptococcus mutans were measured by both monoclonal antibody-based detecting system (Saliva-$check^{TM}$ Mutans) and dip slide detecting system($Dentocult^{TM}$-SM). The results showed that Saliva-$check^{TM}$ Mutans levels had a significant correlation with dfs rate of subjects and the two test kits, Saliva-$check^{TM}$ Mutans and $Dentocult^{TM}$-SM were shown to have a good correlation although they were based on different mechanism.

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