• 미생물학회지
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• 제48권4호
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• pp.328-331
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• 2012

울릉도 연안의 심해(1500 m)에 서식하는 미생물의 다양성을 조사하였다. Ultramembrane filter를 사용하여 해양미생물을 여과한 다음 미생물군집 DNA를 정제하여 16S rDNA를 증폭하였다. Pyrosequencing 방법으로 염기서열을 분석한 결과 총 13,029 reads를 얻었으며, 이중에 54.1%가 uncultured bacteria, 23.4%가 alphaproteobacteria, 22.3%가 gammaproteobacteria이었고 flavobacteria, actinobacteria, epsilonproteobacteria 등이 0.2%이내에서 분포하고 있었다. 울릉도 지역의 해양심층수에서 배양이 가능한 것으로 알려진 미생물로서는 alphaproteobacteria의 Rhodobacteraceae과 (family), gammaproteobacteria의 Alteromonadaceae, Halomonadaceae, Piscirickettsiaceae과가 주로 분포하였다.

• 한국수산과학회지
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• 제43권4호
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• pp.373-379
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• 2010

The artificial upwelling of deep seawater increases primary production. This study conducted a lab-scale experiment to investigate the growth of phytoplankton with the mixing ratio of deep and surface seawater. The chlorophyll content in the sample of pure deep seawater was highest, regardless of the phytoplankton groups. Nutrients contained in the deep seawater positively influenced the growth of phytoplankton. The optimum mixture to apply in an artificial upwelling system was a 1:1 ratio of deep and surface seawater. An experiment considering other environmental conditions, such as luminance and specific gravity, should be performed.

• 한국해양학회지:바다
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• 제4권3호
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• pp.215-225
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• 1999

심해저 표층 퇴적물 중 주원소, 미량원소, 희토류 원소의 수직 함량분포 및 변화의 원인을 구명하기 위해 북동 태평양 클라리온-클리퍼톤 균열대(Clarion-Cliperton fracture zone, C-C 지역) 중남부에 위치한 한국심해환경연구(Korea Deep-sea Environmental Study, KODES) 지역에서 다중 주상 및 상자형 퇴적물 채취기를 이용해 주상시료 6개를 채취 분석했다. KODES 지역 주상 시료는 갈색인 Unit I과 연갈색인 Unit II로 구분되며, Unit I은 함수량이 높은 준액상층(peneliquid layer)인 반면 Unit II는 상대적으로 고화가 진행된 층이다. 두 Unit 간 주원소, 희토류 원소 그리고 Cu, Sr, Rb 등 원소는 함량 차이는 적으나 Mn, Ni, Co 등 원소는 Unit II에 비해 Unit I에서 함량이 2~3배 높다. R-형 요인 분석결과 연구지역 퇴적물은 Al-Ti-K-Mg-Fe-Rb-Ce 등으로 구성된 규산염 상, Ca-P-Cu-Sr-3+REEs(Trivalent Rare Earth Elements)로 구성된 인회석 상, 그리고 Mn-Ni-Co로 구성된 망간산화물 상으로 구분되는데, 규산염 상과 인회석 상은 두 Unit 간 요인점수가 큰 차이를 보이지 않는 반면 망간산화물 상은 Unit I에서 요인점수가 높다. 한편, 두 Unit는 Ni/Cu 비에 의해 구분되는데, Unit I의 Ni/Cu 비가 Unit II보다 2배 높다. 이것은 미세환원환경에서 재동된 후 준액상층인 Unit I에 산화물로 재침전된 Ni, 그라고 유기물과 함께 해저면으로 공급된 후 Unit I에서 유기물이 분해되면서 재동되어 인회석에 포함된 Cu 의 지화학적 거동 차이에 기인한 것으로 해석된다.

• 한국해양학회지:바다
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• 제4권4호
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• pp.390-399
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• 1999

북동태평양 심해저 생물자원 퇴적물에서 서로 다른 두 종류의 측정기 그리고 선상 및 육상 실험실에서 획득한 베인전단강도(vane shear strength) 자료의 비교 연구를 위하여 다중주상시료 채취기(multiple corer)로 규질 및 탄산질 퇴적물 시료를 채취하였다. 전단강도는 $2.0{\times}2.0$ cm의 베인($90^{\circ}$ four blade vane)이 장착된 수동베인측정기(hand-held vane apparatus, 수동베인) 그리고 회전식 점도계 (rotational viscometer)에 $1.0{\times}0.88$ cm의 베인을 장착하여 구성한 전동베인 측정기(motorized shear vane system, 전동베인)로 측정되었다. 수동베인과 전동베인으로 측정된 전단강도 값들은 코어 깊이에 따라 증가하는 동일한 변화 양상을 보이며, 절대 값도 유의한 차이를 보이지 않는다. 그리고 선상에서 측정된 전단강도 값에 비하여 3개훨월후 실험실에서 동일 장비에 의해 측정한 값들 또한 큰 차를 나타내지 않는다. 한편, 실험실에서 전동베인으로 동일한 코어를 2~4회 반복 측정한 결과 동일한 깊이에서도 측정 지점(hole)에 따라 차이가 나타난다. 또한 한 정점 내에서 서로 다른 코어 시료중 전단강도 값은 상당한 차이를 보이는 경우도 있다. 이는 심해저 주상 퇴적물의 물리적 특성이 생물 교란 작용 등의 영향으로 국부적으로 다르기 때문에 나타난 결과로 판단된다. 즉 심해저 퇴적물의 전단강도는 선상에서 또는 3개월이 지난 후 실험실에서, 그리고 두 종류의 기기를 사용하여도 깊이에 따른 유의한 차이를 보이지 않고, 오히려 한 코어시료 내의 국부적 물성차이 또는 한 정점 내 지점별 물성차이에 의한 변화 폭이 더 크게 나타낸다.

• 생명과학회지
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• 제18권3호
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• pp.381-386
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• 2008

동해에서 취수한 해양심층수의 암예방 효능을 얄아보고자 암발생 억제물질의 생화학적 표식자(biochemical markers)인 CYP 1A2 활성, phase II 효소인 QR과 GST의 활성, GSH 함량 및 ODC 활성에 미치는 영향을 살펴보았다. 해양심층수는 암의 개시단계(initiation)를 유발시키는 것으로 알려진 CYP 1A2 활성을 경도의존적으로 저해시켰다. 해양심층수를 Hepa 1clc7 세포에 경도별$(100{\sim}1,000)$로 처리하였을 때 phase II 생체 해독효소인 QR과 GST의 활성은 최대 20%의 증가를 나타내었고, 외부의 독성물질이나 대사산물로부터 세포를 보호하는 역할을 하는 GSH의 함량은 $26{\sim}40%$의 증가를 나타내었다. 그리고 발암과정의 촉진/진행단계에 관여하는 ODC의 활성은 해양심층수의 경도 800과 1,000에서 20%와 35%의 저해율을 나타내었으며, 경도 1,000을 처리한 군에서는 양성대조군인 DFMO와 같은 저해율을 나타내었다. 그러므로 본 연구 결과에 의하면 동해 해양심층수는 발암과정과 관련된 개시 및 촉진/진행단계를 저해시켜 암예방 효능을 나타낼 것으로 사료된다.

• 수산경영론집
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• 제1권2호
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• pp.1-22
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• 1970

Our fisheries protucts industry has developed rapidly during past 10 years ; production was about twice: fihing fleets increased twice in number and 3 times in tonnage ; export was 5 times. Govermment is trying to develop deep sea fisheries in order to surmount the depression of coast fisheries. At present more than 270 deep sea fishing boats are working with superior skill to other country at the South Pacific, the Atlantic, the Indian, and the North Pacific Ocean. Our deep sea fisheries is consisted of the tuna long line and the trawler. The tuna long line of them has 230 crafts in 1969 ana the deep sea trawler has 40 crafts, too. Comparing to 1962, the number of the deep sea fishing boats has been increased highly to 54 times, 7.71 times average per year increasing rate. The rate of the tuna long line to the trawler at the end of 1969 shows 85:15, More than half of them are 100~200 (equation omitted), if we classify them according to (equation omitted) or boat craft. 70% of them has less than 5 years ships age. The Korean Marine Industry Development Corporation has more than 1/3 fishing fleets, with 91 crafts, if We consider it according to corporation. Considering it according to the financial resources, dependence upon foreign loan is as high as 88%. Catches was 74, 450 M/T ($24, 663, 000)at the end of November in 1969 and it was increased to 113.5 times in catch amount and 118 times in value, comparing to those of 1962. Considering it according to the ocean, the order is arranged to the Pacific, the Atlantic, and the Indian Ocean. The average production amount of each craft is 250~400 S/T a year. The result of export took up 1/3 of total fisheries product export with $ 22, 398, 000 at the end of november in 1969. Employee cost of fishing coast is 8% higher than other fishing. The profit is highest in our fisheries. Most of the products except the trawler fishing are sofa at the fishing grounds to the processing company, and they lose much money. They buy most of bait from Japan, giving $8~10 for 1C/S(10kg). Fish price is $390~520 according to the kind of fish for S/T at the fishing grounds, and the rapid frozen fishes brought to Japan are about $ 800 for S/T. There is much difference. in price. Problems 1. Want of self capital. 2. To get the refrigeration boats enough. 3. International Fishing Regulation. 4. To get high price and to secure consuming grounds. 5. To get home-production of bait. 6. To exploit new fishing grounds. 7. To get larger boats. 8. To get mariner enough. When the problems mentioned above are solved, the Deep Sea Fisheries of oun courtry will be developed more largely.

• 한국해양공학회지
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• 제13권2호통권32호
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• pp.116-128
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• 1999

A deep water wave prediction model applicable to the East Sea is presnted. This model incorporates rediative transter of energy specrum, atmospheric input form the wind, nonlinear interaction, and energy dissipation by white capping. The propagation scheme by Gadd shows satisfactory results and the characteristics of the nonlinear interaction is simulated well by discrete interaction approximatiion. The application of the model to the sea around the Korean Peninsula shows reasonable agreement with the observation.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.952-958
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• 2004

Of 23 psychrotrophic bacteria isolated from the west Pacific deep-sea sediments, 19 were assigned to the $\gamma$-Proteobacteria, 3 to the <$\beta$-Proteobacteria, and 1 to the Gram-positive bacteria, as determined by their 16S rDNA sequences. Ten psychrotrophs, affiliated to the Psychrobacter, Pseudoalteromonas, and Pseudomonas genera in the $\gamma$-Proteobacteria group, were screened for lipolytic bacteria. The majority of the lipolytic isolates had growth temperatures between 4-$30^\circ{C}$, and all of them were neutrophilic, aerobic, or facultatively anaerobic, and some were able to produce multiple kinds of ectohydrolytic enzymes. The deep-sea strains Psychrobacter sp. wp37 and Pseudoalteromonas sp. wp27 were chosen for further lipase production analysis. Both strains had the highest lipase production when grown at 10 to $20^\circ{C}$; their highest lipase production occurred at the late-exponential growth stage; and the majority of the enzymes were excreted to the outside of the cells. Lipases from both strains had the same optimal reaction temperature and pH (20-$30^\circ{C}$, pH 7-8) and could retain about 60% of their highest activity at $4^\circ{C}$. Furthermore, SDS-PAGE and an in-gel activity test showed that they had the same high molecular mass of about 85 kDa.

• 한국자원식물학회지
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• 제19권6호
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• pp.687-691
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• 2006

This experiment was conducted on rice (cv. 2005 Thaoi) seeds to study whether priming with deep sea water (DSW) results in enhancement of seed emergence and seedling growth and to identify the optimum concentration of Deep Sea Water (DSW) for priming. Two experiments were conducted subsequently. In experiment 1, four concentrations of the DSW (10%, 20%, 30% and 40%), and in experiment 2, five concentrations of DSW (10%, 15%, 20%, 25% and 30%) were prepared and seeds were primed for 24 hours duration at $25^{\circ}C$. Beside this, hydro priming with plain water was also included as a control. Experiments were laid out in Completely Randomized Design (CRD) with three replications. Result showed that 20% DSW seed priming treatment had improved the emergence, seedling height, number of roots and root length as compare to other with DSW or without DSW treatments. Beyond 20% DSW priming (i.e. 25%, 30% and 40%) were not suitable for priming the seed. On the basis of seedlings growth parameters; emergence, seedling height, root number and length, and shoot root ratio, 20% DSW priming was the best priming treatment.

• 한국자원식물학회지
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• 제25권3호
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• pp.323-328
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• 2012

This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at $25^{\circ}C$ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.