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Transcriptome analysis of the livers of ducklings hatched normally and with assistance

  • Liu, Yali;He, Shishan;Zeng, Tao;Du, Xue;Shen, Junda;Zhao, Ayong;Lu, Lizhi
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.6
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    • pp.773-780
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    • 2017
  • Objective: "Hatchability" is an important economic trait in domestic poultry. Studies on poultry hatchability focus mainly on the genetic background, egg quality, and incubation conditions, whereas the molecular mechanisms behind the phenomenon that some ducklings failed to break their eggshells are poorly understood. Methods: In this study, the transcriptional differences between the livers of normally hatched and assisted ducklings were systematically analyzed. Results: The results showed that the clean reads were de novo assembled into 161,804 and 159,083 unigenes (${\geq}200-bp$ long) by using Trinity, with an average length of 1,206 bp and 882 bp, respectively. The defined criteria of the absolute value of log2 fold-change ${\geq}1$ and false discovery rate${\leq}0.05$ were differentially expressed and were significant. As a result, 1,629 unigenes were identified, the assisted ducklings showed 510 significantly upregulated and 1,119 significantly down-regulated unigenes. In general, the metabolic rate in the livers of the assisted ducklings was lower than that in the normal ducklings; however, compared to normal ducklings, glucose-6-phosphatase and ATP synthase subunit alpha 1 associated with energy metabolism were significantly upregulated in the assisted group. The genes involved in immune defense such as major histocompatibility complex (MHC) class I antigen alpha chain and MHC class II beta chain 1 were downregulated in the assisted ducklings. Conclusion: These data provide abundant sequence resources for studying the functional genome of the livers in ducks and other poultry. In addition, our study provided insight into the molecular mechanism by which the phenomenon of weak embryos is regulated.

Rosenvingea orientalis (Scytosiphonaceae, Phaeophyceae) from Chiapas, Mexico: life history in culture and molecular phylogeny

  • West, John A.;Zuccarello, Giuseppe C.;Pedroche, Francisco F.;De Goer, Susan Loiseaux
    • ALGAE
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    • v.25 no.4
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    • pp.187-195
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    • 2010
  • The genus Rosenvingea is well known in the tropics. Four species have been reported from Pacific Mexico: R. floridana, R. antillarum, R. intricata and R. sanctae-crucis. We collected a plant (Boca del Cielo, Chiapas) that we identified as Rosenvingea orientalis, a species not previously reported from Pacific Mexico. We were able to characterize the life cycle of this species for the first time in laboratory culture. It reproduced exclusively by plurilocular sporangia (plurangia). The mature plants were up to 6 cm long with cylindrical to compressed fronds (to 2 mm wide) with dichotomous branches in the upper half of the thallus. The medulla was hollow with 2-3 layers of large inflated colourless cells at the periphery. The cortex was comprised of 1 layer of small cells, each with a single chloroplast and pyrenoid. Linear plurangial sori with phaeophycean hairs formed along the mature fronds. Zoospore germlings developed into prostrate filamentous systems, each with a single phaeophycean hair that gave rise to a single erect shoot with multiple hairs arising near the tip. Molecular phylogeny using the psaA gene placed this isolate within the Scytosiphonaceae. It does not confirm the exact identification of R. orientalis, although its placement close to other Rosenvingea sequences was confirmed and morphological evidence supports its placement in R. orientalis. Our culture investigations indicated that it has an asexual life cycle. Further collections are needed to resolve the full generic and specific relationships of Rosenvingea and related taxa, and their reproductive patterns.

The Larval Development of Pagurus lanuginosus do Haan(Crustacea, Anomura) Reared in the Laboratory (PAGURUS LANUGINOSUS DE HAAN의 유생 발생에 관한 연구)

  • HONG Sung Yun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.1-15
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    • 1969
  • A study on the complete zoeal development and the glaucothoe of Pegurus lenuginosus de Haan wvas conducted in the laboratoy. 1) P. lanuginosus had four zoeal stages and a single glaucothoe stage. 2) At mean temporature $13.6^{\circ}C$ and mean salinity $33.67\%_{\circ}$, total duration of the zoeal stages was approximately 31-32 days. 3) The zoeal larvae of P. lanuginosus can be easily distinguished from those of other Pagurus species in having 7+7 telson processes of which number as well as shape is consistent throughout all the zoeal stages. 4) The antennal exopodite bears five pulmose setae, and the endopodite is naked throughout the zoeal stages. 5) The 6th abdominal segment has no mid_dorsal spine on its posterior margin during the 3rd and 4th stages. 6) The basipodites of uropod are naked on the distal margin. 7) Mandibular palp is absent in the 4th stage. 8) Antenna of glaucothoe is long extending far beyond the chelipeds. 9) Ocular scale is absent in the glaucothoe. 10) Glaucothoe has 3+3 telson processes on the distal margin of the telson.

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Effect of Long-Term Pyridoxine Depletion on Lipid Composition in the Developing Rat Brain (장기간의 Pyridoxine 부족이 새끼쥐 뇌의 지방조성에 미치는 영향)

  • 김정희
    • Journal of Nutrition and Health
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    • v.20 no.5
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    • pp.318-329
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    • 1987
  • Weanling female Sprague Dawley rate were fed 1.2mg pyridoxine' HCl/kg diet Cdepleted diet) and 2 22mg pyridoxine' HCI/kg dietCcontrol diet). The control and one depleted group were fed their diets throughout growth, gestation, and lactation. Other three depleted groups were fed the depleted diet throught growth and gestation, and then pyridoxine w was supplemented by feeding control diet at 5, 10, and 21 days postpartum. The brains were analyzed for proteolipid protein, cholesterol, and cerebrosi­d de. Percentage stimulation of erythrocyte alanine aminotransferase activity was also determined. Body and brain weight were significantly lower at all ages in depleted group than the control and depleted group showed inadequacy of B6 at all ages. PProteolipid protein and cholesterol were significa­n ntlylower in the depleted group at 10, 21, 35 and 5 50 days. The postnatal development of cerebroside in brain was delayed in depleted groups suppleme­I nted at 5, 10, and 21 days. When supplementation was initiateo at 5 days postparturn, contents of cho­lesterol proteolipid protein were reversed. But some differences in brain development of pups we­re evident when supplementation of dams was de­layed to 10 days or 21 days.

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Orienting the superficial inferior epigastric artery (SIEA) pedicle in a stacked SIEA-deep inferior epigastric perforator free flap configuration for unilateral tertiary breast reconstruction

  • Yu, Ya-han;Ghorra, Dina;Bojanic, Christine;Aria, Oti N.;MacLennan, Louise;Malata, Charles M.
    • Archives of Plastic Surgery
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    • v.47 no.5
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    • pp.473-477
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    • 2020
  • Superficial inferior epigastric artery (SIEA) flaps represent a useful option in autologous breast reconstruction. However, the short-fixed pedicle can limit flap inset options. We present a challenging flap inset successfully addressed by de-epithelialization, turnover, and counterintuitive rotation. A 47-year-old woman underwent left tertiary breast reconstruction with stacked free flaps using right deep inferior epigastric perforator and left SIEA vessels. Antegrade and retrograde anastomoses to the internal mammary (IM) vessels were preferred; additionally, the thoracodorsal vessels were unavailable due to previous latissimus dorsi breast reconstruction. Optimal shaping required repositioning of the lateral ends of the flaps superiorly, which would position the ipsilateral SIEA hemi-flap pedicle lateral to and out of reach of the IM vessels. This problem was overcome by turning the SIEA flap on its long axis, allowing the pedicle to sit medially with the lateral end of the flap positioned superiorly. The de-epithelialized SIEA flap dermis was in direct contact with the chest wall, enabling its fixation. This method of flap inset provides a valuable solution for medializing the SIEA pedicle while maintaining an aesthetically satisfactory orientation. This technique could be used in ipsilateral SIEA flap breast reconstructions that do not require a skin paddle, as with stacked flaps or following nipple-sparing mastectomy.

Cloning and Expression of the Duck Leptin Gene and the Effect of Leptin on Food Intake and Fatty Deposition in Mice

  • Dai, Han Chuan;Long, Liang Qi;Zhang, Xiao Wei;Zhang, Wei Min;Wu, Xiao Xiong
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.6
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    • pp.850-855
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    • 2007
  • Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of leptin and its function in poultry is very poor. In this study, the duck leptin gene was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) from duck liver RNA. The cDNA fragment was inserted into the pET-28a expression vector, and the resulting plasmid was expressed in Escherichia coli BL21 (DE3). Experimental mice were given an intraperitoneal injection of 10 mg/kg leptin dissolved in phosphate buffered saline (PBS), while the control mice were injected with PBS. The effect of leptin on food intake, body weight and fatty deposition in mice was detected. Sequence analysis revealed that duck leptin had a length of 438 nucleotides which encoded a peptide with 146 amino acid residues. The sequence shares highly homology to other animals. The coding sequence of duck leptin was 84 and 86% identical to human and pig leptin nucleotides sequence. Highest identity was with the rat coding sequence (95%). The identity of the amino acid sequence was 84, 82 and 96% respectively compared to that of the human, pig and rat. Results of SDS-PAGE analysis indicated that a fusion protein was specifically expressed in E. coli BL21 (DE3). The purified product was found to be biologically active during tests. Continuous administration of recombinant duck leptin inhibited food intake. Despite the decrease of food intake, leptin significantly induced body weight and fatty deposition. These changes were accompanied by a significant down-secretion of plasma glucose, cholesterol, triglyceride and insulin levels in mice. The observations provide evidence for an inhibitory effect of leptin in the regulation of food intake and for a potential role of duck leptin in the regulation of lipogenesis.

A Study on the Upward Flow of the Majo and Maja Costumes in 18th Century Spain, Observed in the Paintings of Francisco de Goya (프란시스코 데 고야의 회화에 나타난 18세기 스페인 마호(majo), 마하(maja) 서민복식의 상향전파 사례연구)

  • Bae, Soojeong
    • Journal of the Korean Society of Clothing and Textiles
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    • v.42 no.1
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    • pp.74-87
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    • 2018
  • This thesis investigated majo and maja costumes of depicted in Francisco de Goya's paintings from the middle of 18th to the early 19th century, and presented the costumes defined as the upward flow from the lower classes to the upper classes. The study methodology selected those describing the maja and majo among the figure paintings, portraits and genre paintings by Goya that identify unique characteristics with the qualitative analysis of the cases of the upward flow with that affect the costume of the upper classes. The influence of the majo and maja costumes were outstanding in the shape of the outfits while the French style gown of the upper class women were one-piece dresses in shape, those of the maja were two-piece dresses that consisted of the upper and gather skirts. The costume was perfected by use of the mantilla or head kerchief, instead of the headdresses prevalent at those times. The majo's costume affected the men's costume of the upper classes in the shape of the outfits. Short coat and tight breeches were preferred to the loose ones as well as instead of a long coat. Rather than the wigs or tri-corn hat, the head kerchief and sash belt applied, which could be evidence of the upward flow as well as shows proof of the influence of majo and maja costumes.

Cytogenetic and Genetic Mutation Features of de novo Acute Myeloid Leukemia in Elderly Chinese Patients

  • Su, Long;Li, Xian;Gao, Su-Jun;Yu, Ping;Liu, Xiao-Liang;Tan, Ye-Hui;Liu, Ying-Min
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.2
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    • pp.895-898
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    • 2014
  • Objectives: The present study aimed to examine the cytogenetic and genetic mutation features of acute myeloid leukemia (AML) in elderly Chinese patients. Methods: A retrospective analysis of cytogenetics and genetic mutations was performed in 113 cases (age range 50-82 years) with de novo AML. Results: The most frequent cytogenetic abnormality was t (15;17) (q22;q21), detected in 10.0% (n = 9) of successfully analyzed cases, followed by t (8;21) (q22;q22) in 8.89% (n = 8), and complex karyotypes in 5.56% (n = 5). Those with complex karyotypes included 4 cases (4.44%) of monosomal karyotypes. The frequencies of NPM1, FLT3-ITD, c-kit, and CEBPA mutations were 27.4% (31/113), 14.5% (16/110), 5.88% (6/102), and 23.3% (7/30), respectively. The complete remission rates of patients in low, intermediate, and high risk groups were 37.5%, 48.6%, and 33.3%, respectively (${\chi}^2$ = 0.704, P = 0.703) based on risk stratification. Conclusion: Cytogenetics and genetic mutations alone may not be sufficient to evaluate the prognoses of elderly AML patients. The search for a novel model that would enable a more comprehensive evaluation of this population is therefore imperative.

A Study on the Deactivation of Commercial DeNOx Catalyst in Fired Power Plant (화력발전소 상용 탈질 촉매의 활성저하 원인에 관한 연구)

  • Park, Kwang Hee;Lee, Jun Yub;Hong, Sung Ho;Choi, Sang Hyun;Hong, Sung Chang
    • Applied Chemistry for Engineering
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    • v.19 no.4
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    • pp.376-381
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    • 2008
  • The deactivation of $V/TiO_2$ catalyst used in SCR (Selective Catalytic Reduction) using ammonia as a reductant to remove the nitrogen oxides (NOx) in the exhaust gas from fired power plant has been studied. The activity and surface area of the catalyst (Used-cat) which was exposed to the exhaust gas for long period have considerably decreased. The characterizations of these SCR catalysts were performed by XRD, FT-IR, FE-SEM, and IC/ICP. The crystal structure of $TiO_2$ both fresh and used catalyst has not been changed. However, $(NH_4)HSO_4$ deposited on the used catalyst surface verified from FT-IR, FE-SEM, and IC/ICP analysis. Moreover, the durability of $SO_2$ was increased by diminishing sulfate ($SO_4^{-2}$)f form.

From a Young Mathematics Professor to a Great Mathematics Teacher: Considering Characteristic Features of the Education of Pure Mathematics in the Social, Institutional and Interdisciplinary Contexts of UCL (드 모르간이 위대한 수학 교육자가 되기까지 : UCL의 사회적, 제도적, 간학문적 맥락 속에서 순수 수학 교육 바라보기)

  • Cho, Su-Nam
    • Journal for History of Mathematics
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    • v.24 no.3
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    • pp.109-143
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    • 2011
  • Augustus De Morgan became to be deeply interested in the education of pure mathematics since he came to teach in UCL because of the specific nature of natural philosophy lectures, the academical knowledge and reasoning powers of the students, and the negative attitudes of London society on mathematics. During his long tenure, he really tried his best to make his students understand the important concepts and the principles of pure mathematics, and logically explain the processes of inducing and proving the laws of pure mathematics. When he could not stay as a mere researcher, he had to concern himself with and pay attention to the problems of educating students. And then his teaching style was constructed in a specific way by the various attitudes about mathematics, the boundary relationship between the adjacent academical branches, and the social and systematic nature of UCL.