• Korean Journal of Ecology and Environment
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• v.48 no.1
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• pp.32-37
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• 2015

Naturalized plants are invaded through various routes and distributed throughout the nation. They are also invaded in the sand dune ecosystem and poses a threat ecological niche of indigenous sand dune plants. We studied the effects of salt stress on the plant growth to suggest the management plans of naturalized plants in the sand dune ecosystem. As a result of germination experiment according to the salt gradient, germination rate decreased as the higher concentration and tolerance range was up to 160 mM. As a result of seedling experiment according to salt gradient, mortality rate was up to 90% from 0 mM to 200 mM and 100% over 300 mM. The day of first death with different concentration was 18.5 days in 100 mM and 6 days in 800 mM since the experiment has started. In addition, the day of last death with different concentration was 29.5 days in 100 mM and 6 days in 800 mM. Therefore, two stages of management plans would be effective. The first one is to suppress the germination by salt spray over 170 mM before germination. The other one is to wither seedlings in a short period by salt spray over 600 mM.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.23-27
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• 1988

This experiment was conducted to determine germination ability, life cycle, regrowth ability of Portulaca oleracea L. The newly harvested seeds from P. oleracea flowered before July 15 were able to germinate, but percent germination decreased markedly as the flowering time was later than July 15, showing no germination of seed collected at Sept. 12 which were flowered at August 30, 76.5% of seeds can be germinated in the light as seed stored in room condition for 90 days, but in dark condition, it needed 2 years of seed storage for germination. Low temperature treatment at $2^{\circ}C$ for 5 days enhanced seed germination of P. oleracea in both light and dark conditions. The maximum vagetative growth was observed at 30 to 75 days after seeding. The late seeding time such as July 1 shortened the period of vegetative growth. However, regardless of the seeding times, the first flowering was observed at about 40 days after seeding. Leaf numbers, shoot lengths, fresh and dry weights were greatly affected by the seeding dates, showing that the earlier seedings produced significantly higher shoot length, fresh and dry weight, leaf numbers and branch numbers than those of the late seedings. When all branches were removed on 68 days after seeding, their regrowth ability was 50.3% and cuttaged branches showed 78.1% regrowth as compared to intact plant.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.221-226
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• 2017

The objective of this study was to find out the most optimal bulbil size and planting densities of garlic bulbils to germination for production of leaf garlic. In the first this experiment, the bulbils classified by the size of bulbil to Big (>0.2) and Small (${\leq}0.2$), were planted at $13,680bulbils/m^2$ and $18,240bulbils/m^2$ (Big), $18,240bulbils/m^2$ and $22,800bulbils/m^2$ (Small), respectively. The germination rate, plant height and total yield were measured. In the second experiment, length, width, fresh weight and dry weight of 200 garlic bulbils were measured to analyze the correlation of days to first germination, T50 and germination rate. In the first experiment, the germination rate showed the highest at Big bulbils. The density of $18,240bulbils/m^2$ was the most suitable for cultivation because of the highest germination rate. In the case of yield, it was found that both of the fresh weight and the dry weight of Big bulbil was 2.8 times heavier than Small bulbil. Therefore, Big bulbil with the density of $18,240bulbils/m^2$ was the most suitable for cultivation because of the highest yield. The correlation between each factor of morphological characteristics had a positive correlation between fresh weight, dry weight and width. Also, the germination rate had a positive correlation with fresh weight and width. And, days to first germination and T50 had a negative correlation with fresh weight and width. In conclusion, the optimal planting density is $18,240bulbils/m^2$ and the optimal size of bulbil was 0.2g or more. However, thicker and heavier bulbils could lead to higher and faster germination.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.303-303
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• 2022

The purpose of this study was to evaluate agronomic characteristics and identify the useful traits to utilize the wheat genetic resources for breeding programs by understanding the phenotypic variation among germplasm through multivariate analysis. In this study, a total of 394 wheat accessions were characterized for 15 agronomic traits using the National Agrobiodiversity Center (NAC) descriptor list, of which 31 accessions from 6 species and 363 unidentified accession (Triticum spp.) available at the NAC, Rural Development Administration (RDA), Korea. Growth characteristics such as leaf width, culm length, spike length, spikelet length, solid stemmed, days to heading, days to maturity, grain-filing period, and also seed characteristics such as width, height, area, perimeter, circle, solidity, and germination percent were studied. Among the 15 agronomic characteristics, the germination percent showed the smallest variation between resources (CV = 0.4%), and the spikelet length (CV = 66.5%) showed the highest variation. A strong positive correlation was found between seed traits such as seed height and seed area (r = 0.90), seed height and seed perimeter (r = 0.87) and seed length and width (r = 0.80). Principal component analysis (PCA) was conducted and the first five principal components comprised 76.7% of the total variance. Among the first five PCs, PCI accounted for 28.5% and PC2 for 20.0%. Wheat resources (394) were classified into four clusters based on cluster analysis, consisting of 215 resources(I), 117 resources(II), 48 resources(III), and 14 resources(IV). Among the clusters, the resources belonging to Cluster III showed the lowest seed width, height, area, and perimeter characteristics compared to other clusters. The wheat resources belonging to cluster IV had small seed width and low germination percent, but took longer to form heads and mature than resources in other clusters. These results will serve as the basis for further genetic diversity studies, and important agronomic characteristics will be used for improving wheat, including developing high-yielding and resistant varieties to biotic and abiotic stresses via breeding programs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.18-24
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• 2018

This study was conducted to use fundamental data about functional foods based on pigmented rice varieties-2 types of normal rice, 6 types of black rice-to analyze changes in chemical component activity during germination. The results are summarized as follows: Germination rates of the pigmented rice varieties were the highest at $30^{\circ}C$. There was a tendency for crude protein contents to increase under the influence of germination periods, and the crude protein contents were the highest on the $6^{th}$ day. Crude fat contents were the highest on days 6-8. Nevertheless, the crude fat contents in pigmented rice decreased during the first 2 days, and subsequently, the contents increased steadily. Crude fiber contents were the highest on the $8^{th}$ day. However, there was no meaningful tendency. Sucrose, glucose, and fructose contents in the pigmented rice increased during germination. In normal rice, glucose content increased up to the $8^{th}$ day. It was higher than that of brown rice. However, sucrose and fructose contents showed no meaningful tendency. There was a general tendency for GABA, alanine, glutamic acid, asparagine, aspartic acid, and lysine contents in the pigmented rice to increase under the influence of germination periods. Aspartic acid and asparagine contents were shown to decrease, compared with that in pre-germinated normal rice. In all varieties, lysine was detected from the second germination day onwards, and on the $6^{th}$ day after germination, its contents were 6.24 mg/100 g and 15.58 mg/100 g in normal rice and pigmented rice, respectively.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1092-1096
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• 1998

This work was to study the possibility of using germinated colored rice as a raw material for sikhe. Colored rice was soaked in water at $15^{\circ}C$ for 2 days and then germinated at 15, 20, 25 and $30^{\circ}C$ for upto 10 days. The higher the germination temperature, the higher the germination speed, the rate of increase in ${\alpha}-amylase$ activity, and the rate of increase in extract and its sugar content. The viscosity of extract rapidly decreased first and then slightly increased during germination. The germination speed of colored rice was lower than that of brown rice at $25^{\circ}C$. The germinated colored rice can be used for sikhe not as a saccharifying agent but as a substitute for white rice for the nutritional purpose.

• Mycobiology
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• v.40 no.2
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• pp.111-116
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• 2012

Ten strains of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were evaluated to find the most effective strain for optimization studies. The first criterion tested for strain selection was the mortality (> 50%) of Spodoptera litura larvae after inoculation of the fungus for 4 days. Results on several bioassays revealed that B. bassiana BNBCRC showed the most virulence on mortality S. litura larvae (80% mortality). B. bassiana BNBCRC also showed the highest germination rate (72.22%). However, its conidia yield ($7.2{\times}10^8$ conidia/mL) was lower than those of B. bassiana B 14841 ($8.3{\times}10^8$ conidia/mL) and M. anisopliae M6 ($8.2{\times}10^8$ conidia/mL). The highest accumulative radial growth was obtained from the strain B14841 (37.10 mm/day) while the strain BNBCRC showed moderate radial growth (24.40 mm/day). M. anisopliae M6 possessed the highest protease activity (145.00 mU/mL) while M. anisopliae M8 possessed the highest chitinase activity (20.00 mU/mL) during 96~144 hr cultivation. Amongst these criteria, selection based on virulence and germination rate lead to the selection of B. bassiana BNBCRC. B. bassiana B14841 would be selected if based on growth rate while M. anisopliae M6 and M8 possessed the highest enzyme activities.

• Korean Journal of Human Ecology
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• v.14 no.3
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• pp.485-489
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• 2005

The purpose of this study is to investigate changes of isoflavone and nutrients during germination of soybean varieties and to determine the optimal germination condition for soybean processing. After 5 days' germination, water content of the soybeans increased in the order of Poongsannamoolkong> Sinpaldal#2> Black#1> Jinpumkong. Ash content was higher in the order of Poongsannamoolkong> Sinpaldal#2> Jinpumkong> black#1 soybean. All varieties of the soybean samples increased in ash content during germination. Crude fat content was higher in the order of Poongsannamoolkong> Black#1> Jinpumkong> Shinpaldalkong. Crude protein content was higher in the order of Shinpaldalkong> Black#1> Jinpumkong> Poongsannamoolkong. Polypeptide subunits separated from the SDS-PAGE of the entire proteins were showing comparatively high level in the range of 2-16KD in Jinpumkong and 2-21KD in Poongsannamoolkong till the first day of germination, 2-25KD in Sinpaldal#2 till the second day, and 6-27KD in Black#l till the third day. Changes of total isoflavone contents during germination showed different patterns in soybean varieties: Jinpumkong and Black#1 had the highest total isoflavone content on the fourth day of germination, Poongsannamoolkong and Sinpaldal#2 on the first and the second day of germination, respectively.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.101-106
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• 1997

This study was carried out to examine the possibility of using brown rice or paddy as raw materials for sikhe. Brown rice and paddy were soaked in water at $15^{\circ}C$ for 2 days and then germinated at $15,\;20,\;25,\;30^{\circ}C$ for upto 10 days. The higher the germination temperature, the higher the germination speed, the increase of ${\alpha}-amylase$ activity, and the increase of extract amount and its sugar content. The viscosity of extract rapidly decreased first and then slightly increased during germination. The activities of ${\alpha}-amylase$ of germinated brown rice and paddy were much smaller than those of germinated barley; however, the extract amount and its sugar content of germinated brown rice and paddy were similar to those of germinated barley. The germinated brown rice can be used for sikhe not as a saccharifying agent but as a substitute for white rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.123-137
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• 2015

This study was carried out to investigate the effect of pre-germination soaking on germination in 90 Korean soybean varieties and identification of protein in seeds of 7 soybean varieties. The results obtained that germination rate of soybean seeds was decreased as amount of soaking water and soaking duration in number of days. Difference in germination rate of soybean seeds was significant at three days soaking with water volume of 90 ml. Water absorption of seeds was rapidly increased during the first 6 hours, followed by slow increase until 24 hours and then decreased 24 to 48 hours after soaking soybean varieties for bean sprout soaked the lowest amount of water, while soybean varieties for cooking with rice showed the lowest seed water content. Dissolved oxygen (DO) in soaking water was rapidly decreased during the first 3 hours after soaking, and then slowly decreased. Soybean varieties for vegetable and early maturity showed the lowest DO during early soaking periods, but showed higher DO after 24 hours than other groups of soybean varieties. Electrical conductivity and Total Dissolved Solid (TDS) were increased as number of soaking days increased. Soybean varieties for vegetable and early maturity showed the highest electrical conductivity and TDS, followed by those for sauce and paste or cooking with rice, while showed the lowest electrical conductivity and TDS, varieties for bean sprout. Among 90 Korean soybean varieties, varieties which showed the highest germination rate were Jangsu-kong for sauce and paste, Sobaegnamul-kong for bean sprout, Seonheuk-kong for cooking with rice, Seunnokkong for vegetable and early maturity. On the ather hand varieties which showed the lowest germination rate were Iksan and Songhak-kong for sauce and paste, Pangsa-kong for bean sprout, Jinyeul-kong for cooking with rice, Sinlok-kong for vegetable and early maturity. Germination rates of soybean seeds were higher when electrical conductivity, TDS and water absorption of seeds were lower. There were negative correlations between electrical conductivity, TDS and water absorption of seeds and germination rate, while there were positive correlations among electrical conductivity, TDS and soybean seed weight.