• Title/Summary/Keyword: Dao

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Effect of 'DaoCao' on the inflammatory cytokines in Human Jurkat cell and THP-1 cell (나초(糯草) 에탄올추출물이 Human 유래 Jurkat 세포와 THP-1 세포의 알러지 및 염증 사이토카인에 미치는 영향)

  • LEE, Young Keun;KIM, Cheong Taek;ROH, Seong Su;CHOI, Hak Joo
    • The Korea Journal of Herbology
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    • v.30 no.5
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    • pp.45-49
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    • 2015
  • Objectives : The aim of this study is to investigate anti-inflammatory activity using various extracts of rice straw (DaoCao) extract (RS).Methods : To investigate the anti-inflammatory effect of RS, we examined the effect of RS on cytokines production on THP-1 cell. Cells were cultured in incubator (37℃, CO25%, 0.5% FBS-RPMI, 1X106cells/ml). One hour after,Dermatophagoides pteronissinus(Dp., 10 ug/ml) was treated into cell and at 6 hour after, each different concentrations(0.1, 1 and 10 ug/ml) of RS were treated. The cells were incubated for 16 hours and harvest the supernatant. The levels of IL-4, IL-5, IL-6, IL-8, MCP-1 and TNF-αwere determined using a commercially available ELISA kit.Results : We investigated whether RS has the inhibition of inflammatory response in Jurkat cells and THP-1 cells. RS suppressed secretion of IL-4, IL-5, and TNF-αinduced by house dust mites in Jurkat cells. It showed significant effects for all concentrations. RS suppressed the increased expression of IL-6, IL-8 and MCP-1 after treatment with mite in THP-1 cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property.Conclusions : RS showed significant biological activities with anti-inflammatory in the human T cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property. In terms of Korean traditional medicine, we expect the results to contribute to building of EBM (Evidence-Based Medicine).

Effect of Strontium on Polyamine Synthesis and Diamine Oxidase during the Germination of Mung Bean (Vigna radiata L.) (숙주의 발아과정 동안 폴리아민 생합성과 Diamine 산화효소에 대한 스트론티움 효과)

  • Kim, Tae-Wan;Kwon, Young-Up;Yun, Seung-Gil
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.6
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    • pp.437-444
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    • 2003
  • Objective of this experiment was to investigate the role of strontium in intracellular processes in mung bean (Vigna radiata L.). Diamine oxidase (DAO) induction by $Sr^{2+}$ appeared to a decrease in putrescine levels correspondently. DAO activities in the hypocotyls were in a range of 0.5 to $1.8unit{\cdot}mg^{-1}\;protein{\cdot}min^{-1}$. The decrease in Put levels in the cotyledons might be partly resulted from Put degradation by DAO. It was observed that the accumulation of spermidine and spermine by $Sr^{2+}$ was in the range of 1 mM to 10 mM. Spermidine levels were 2 to 3 fold higher than in the absence of strontium. The increase in polyamine levels was observed not only on a basis of g fresh weight but also a RNA basis. These results demonstrated that the inhibitory action of $Sr^{2+}$ may be closely related with polyamine metabolism as well as diamine oxidation and polyamine accumulation.

The study of the Huang-Lao philosophy in the Wenzi (『문자(文子)』의 황노사상(黃老思想)에 대한 연구)

  • Kim, Yea-ho
    • The Journal of Korean Philosophical History
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    • no.39
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    • pp.169-194
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    • 2013
  • The Wenzi is the material criticized about the relation bamboo-slip literature and current literatures in the academic world. If is true or not, because we don't know the author and the records accurately. In this study, to sublate the researching method of HuangLao philosophy that vogues the boundary between Zajia, on the basis of volumes with bamboo-slip literature and the present literatures shared, I discuss the peculiarity of Wenzi HuangLao philosophy, comparing Dao-theory of Daoist. The Wenzi is HuangLao philosophy of Rujia pattern that doesn't understand as the Dao-Fa school. And its origin is Laozi philosophy. It accepts Zujia philosophy, putting first in importance about Rujia philosophy. In this study, the origin of the Wenzi is Laozi philosophy theoretically, but I discuss that the Wenzi is the early literature forming Rujia pattern HuangLao philosophy. And the Wenzi tries to combine the theory of Rujia in the social political category.

A Study on the theory of Mind in LüZuqian(呂祖謙) philosophy (여조겸(呂祖謙) 심론(心論) 연구(硏究) : 여조겸과 주희의 사상적 대립과 절충)

  • Yeon, Jae-heum
    • The Journal of Korean Philosophical History
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    • no.38
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    • pp.63-96
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    • 2013
  • $L{\ddot{u}}Zuqian$(呂祖謙) was one of the famous philosophers during the Southern Song period, and is called one of DongNamSanXian(東南三賢) together with ZhuXi(朱熹) and ZhangShi(張?). With his profound knowledge transmitted and uphold by JiaXue(家學), $L{\ddot{u}}Zuqian$(呂祖謙) established his learning system, interacting with scholars of those days. Principally, $L{\ddot{u}}Zuqian$(呂祖謙)'s XinLun(心論) was based on Mengzi(孟子)' theory of LiangXin(良心) and BenXin(本心). $L{\ddot{u}}Zuqian$(呂祖謙) explained the meaning of such a conscience through ChuXin(初心) and Inner NeiXin(內心). According to $L{\ddot{u}}Zuqian$(呂祖謙), ChuXin(初心) arouses when one encounters external things, and this one's intention enables us to make the right judgments over the outside objects. NeiXin(內心) means LiangXin(良心) and BenXin(本心) that recovered the ability of moral awareness. The important significances of $L{\ddot{u}}Zuqian$(呂祖謙)'s XinLun(心論) are XinWai WuDao(心外無道), and XinWaiWuTian(心外無天). Through these, $L{\ddot{u}}Zuqian$(呂祖謙) emphasized that Tian(天), Dao(道), and Li(理) are one. $L{\ddot{u}}Zuqian$(呂祖謙) arranged a meeting of EHuSi(鵝湖寺), and exerted efforts to negotiate the academic differences between ZhuXi(朱熹) and LuJiuyuan(陸九淵). However, compared with LuJiuyuan(陸九淵) who asserted FaMingBenXin(發明本心), $L{\ddot{u}}Zuqian$(呂祖謙) put the emphasis on DaoWenXue(道問學) with self-awareness of conscience. Meanwhile, $L{\ddot{u}}Zuqian$(呂祖謙) valued much of Jing(敬) like ZhuXi(朱熹). But, to $L{\ddot{u}}Zuqian$(呂祖謙), Jing(敬) meant WuJianDuan(無間斷) of ChunYiBuZa(純一不雜) DaoDeXin(道德心), and implied the same as Cheng(誠). $L{\ddot{u}}Zuqian$(呂祖謙) stressed the reading and pursuit of study, however, he also asserted that Li(理) could be understood and realized through self-awareness of one's mind and its reflection, and working without interruption. $L{\ddot{u}}Zuqian$(呂祖謙)'s academic tradition of the XinXueDe XueFeng(心學的 學風), which reveals through compromise and confrontation with ZhuXi(朱熹), can be said that it will have a significant meaning of idealism of dispute in the Southern Song period.

Jo Jeongsan's Religious Activity in the Context of the Social Role of Religion: Focusing on Propagatory Works and Soteriological Aims ('구세제민(救世濟民)'을 통해 본 조정산(趙鼎山)의 포교공부 일고찰 - 강증산 성사(聖師)와의 양산도(兩山道)의 원리와 관련하여 -)

  • Ko Nam-sik
    • Journal of the Daesoon Academy of Sciences
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    • v.47
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    • pp.203-239
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    • 2023
  • Jo Jeongsan's religious activity undertaken to benefit society can be organized into two categories. First, attention can be paid to Jo Jeongsan's religious activity of establishing religious order by enshrining Kang Jeungsan as the God of Ninth Heaven and making the Great Dao of Heaven and Earth the basic idea inspiring the social activity of his order. This was completed through the 50 years of propagation work carried out by Jo Joengsan in accordance with Kang Jeungsa's purpose of saving the world and its inhabitants. Second, his practice of social work in the field of people's lives based on the idea of the Dao can also be observed. This religious activity of Jo Jeongsan which began in 1909 was the sacred manifestation of his will to realize Kang Jeungsan's purpose of saving the world and people, and it was also a practice of spreading virtue throughout the world. In addition, Park Wudang carried out Sihak and Sibeop Gongbu (two varieties of holy work) for the cultivation of Dao trainees, and those methods came from the systematic cultivation practice established by Jo Jeongsan. Regarding this, we can refer to Kang Jeungsan's saying, "As the Dao shall dwell in the 12,000 peaks of Geumgang Mountain, the same number of sages who were enlightened to the Dao will be born into the world." The perfected state of human maturity or the emergence of people who are enlightened to the Dao would be the completion of the idea of saving the world and its inhabitants. Therefore, the holy works of Sihak and Sibeop that are now being carried out can be seen as the continuance of the pursuit of saving the world and humankind, as it was continually upheld by Park Wudang's predecessors, Jo Jeongsan and Kang Jeungsan.

Control of histamine-forming bacteria by probiotic lactic acid bacteria isolated from fish intestine (생선 내장으로부터 분리된 프로바이오틱 유산균에 의한 히스타민 생산균의 제어)

  • Lim, Eun-Seo;Lee, Nahm-Gull
    • Korean Journal of Microbiology
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    • v.52 no.3
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    • pp.352-364
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    • 2016
  • In this study, we examined in vitro the potential probiotic properties of lactic acid bacteria (LAB) obtained from the fish intestine and their ability to degrade histamine through the production of diamine oxidase (DAO) enzymes and bacteriocin. Among 97 LAB strains isolated from the intestine of croaker, flatfish, pollack, and rockfish, CIL08, CIL16, FIL20, FIL31, PIL45, PIL49, PIL52, and RIL60 isolates exhibited excellent survival rates under simulated gastrointestinal tract conditions, high adhesion ability to HT-29 epithelial cells, and resistance to the antibiotics such as amoxicillin, ampicillin, erythromycin, penicillin G, streptomycin, tetracycline, or vancomycin. In addition, these strains did not produce histamine in decarboxylating broth containing histidine. In particular, 4 strains (CIL08, FIL20, PIL52, and RIL60) that may produce DAO were significantly able to degrade histamine. The bacteriocins produced by FIL20, FIL31, and PIL52 LAB inhibited the growth and histamine production of Enterococcus aerogenes CIH05, Serratia marcescens CIH09, Enterococcus faecalis FIH11, Pediococcus halophilus FIH15, Lactobacillus sakei PIH16, Enterococcus faecium PIH19, Leuconostoc mesenteroides RIH25, or Aeromonas hydrophilia RIH28. Histamine-producing strains isolated from fish intestine were found to reduce histamine accumulation during co-culture with CIL08, FIL20, PIL52, and RIL60 LAB showing histamine degradation or bacteriocin production ability. The probiotic strains preventing histamine formation were identified as Pediococcus pentosaceus CIL08, Lactobacillus plantarum FIL20, Lactobacillus paracasei FIL31, Lactobacillus sakei PIL52, and Leuconostoc mesenteroides RIL60 with high similarity based on 16S rRNA gene sequencing.