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http://dx.doi.org/10.6116/kjh.2015.30.5.45.

Effect of 'DaoCao' on the inflammatory cytokines in Human Jurkat cell and THP-1 cell  

LEE, Young Keun (Department of Cosmetic Science, Daejeon Health Science College)
KIM, Cheong Taek (RNS, Ltd.)
ROH, Seong Su (College of Korean Medicine, Daegu Hanny university)
CHOI, Hak Joo (Traditional and Biomedical Research Center(TBRC), Daejeon University)
Publication Information
The Korea Journal of Herbology / v.30, no.5, 2015 , pp. 45-49 More about this Journal
Abstract
Objectives : The aim of this study is to investigate anti-inflammatory activity using various extracts of rice straw (DaoCao) extract (RS).Methods : To investigate the anti-inflammatory effect of RS, we examined the effect of RS on cytokines production on THP-1 cell. Cells were cultured in incubator (37℃, CO25%, 0.5% FBS-RPMI, 1X106cells/ml). One hour after,Dermatophagoides pteronissinus(Dp., 10 ug/ml) was treated into cell and at 6 hour after, each different concentrations(0.1, 1 and 10 ug/ml) of RS were treated. The cells were incubated for 16 hours and harvest the supernatant. The levels of IL-4, IL-5, IL-6, IL-8, MCP-1 and TNF-αwere determined using a commercially available ELISA kit.Results : We investigated whether RS has the inhibition of inflammatory response in Jurkat cells and THP-1 cells. RS suppressed secretion of IL-4, IL-5, and TNF-αinduced by house dust mites in Jurkat cells. It showed significant effects for all concentrations. RS suppressed the increased expression of IL-6, IL-8 and MCP-1 after treatment with mite in THP-1 cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property.Conclusions : RS showed significant biological activities with anti-inflammatory in the human T cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property. In terms of Korean traditional medicine, we expect the results to contribute to building of EBM (Evidence-Based Medicine).
Keywords
Rise straw extract; DaoCao; anti-inflammatory; allergy; Dermatophagoides pteronissinus;
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Times Cited By KSCI : 2  (Citation Analysis)
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