• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.730-736
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• 1995

The solubilization and proteolytic process of $\delta $-endotoxin was analvsed to compare the biochemical property of the toxin isolated from B. thuringiensis subsp. sotto. The purified crystals were dissolved in 50 mM carbonate buffer containing 10 mM dithiothreitol at pH 10 for various times. The electrophoretic pattern showed that a rapid disappearance of 138 kDa protein band. This disappearance of protein with high molecular weight was accompanied by the appearance of new protein fragment with 104 kDa, 60 kDa, and 25 kDa. For proteolvtic processing, the soluble crystals were digested with trypsin for various times. The soluble crystal protein of 104 kDa was completely disappeared. However, the protein fragment of 60 kDa and 25 kDa still remained after complete proteolysis. The comparative immunoblot analysis showed that the antiserum against intact crystals showed strong immunoreactivity to the homologous inclusion protein of 138 kDa, 104 kDa, and 25 kDa, and to the intact spores of 221 kDa and 138 kDa, but not to the vegetative cell homogenate. The sera against crystals and spores had no immunoreactivity to the vegetative cell homogenate.

• Journal of Life Science
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• v.25 no.7
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• pp.765-772
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• 2015

The aim of this study was to determine the effect of decursin (D) and decursinol angelate (DA) against bisphenol A (BPA) toxicity in a rat two-generation study. Adult rats were divided into the following three groups: (1) control, (2) BPA, and (3) BPA+D/DA. The D and DA treatment of F0 parents increased the terminal body weight and relative adult organ weights (testes, kidneys, spleen, and liver) when compared with the BPA group. A significant decrease in sperm count was found in the BPA+D/DA (7.69%) and BPA (64.70%, p<0.01) groups, when compared with the sperm count in the control group. No offspring were obtained in the F1 generation of the BPA (50 mg/kg/day) group, but the addition of D/DA in the BPA+D/DA group significantly restored fertility (55.78%) and gestation indices (98.87%) in the F1 generation. No significant differences were found in the fertility index between the control (75.02%) and the BPA+D/DA (78.11%) groups in the two-generation study, when compared with the one-generation study. The viability ratio during lactation in the D/DA group was also similar to that of the control group. These data indicate that D/DA (50 mg/kg/day) administered over two generations causes significant positive changes in reproductive or developmental parameters.

• Bulletin of the Korean Space Science Society
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• 2000.04a
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• pp.39-39
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• 2000

No Abstract, See Full Text

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.2
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• pp.135-141
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• 2018

This study investigated the immuno-potentiating activities of Japanese mud shrimp Upogebia major. We examined the effects of enzymatic hydrolysate from U. major on the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) and on the expression of pro-inflammation cytokines including $TNF-{\alpha}$, IL-6 and $IL-1{\beta}$ in RAW 264.7 cells. The treatment of six enzymatic hydrolysates of U. major (alcalase, ${\alpha}$-chymotrypsin [${\alpha}-Chy$], trypsin, pepsin, neutrase, protamex) significantly increased the production of NO in RAW 264.7 cells, with ${\alpha}-Chy$ having the greatest effect. This hydrolysate was fractionated by two ultrafiltration membranes at 3 and 10 kDa to created three fractions (below 3 kDa, between 3 and 10 kDa, and above 10 kDa). Of these, the <3 kDa and >10 kDa fractions showed significant increases in NO production. These two fractions also induced $PGE_2$ production in RAW 264.7 cells and showed significant increases in the expression of all cytokines studied. These results suggest that enzymatic hydrolysate from U. major is a potentially useful food material with immune-potentiating effects.

• Molecular & Cellular Toxicology
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• v.5 no.2
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• pp.153-159
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• 2009

In this study, we assessed the acute and subacute toxicity of Angelica gigas Nakai (A. gigas Nakai) extracts, which are comprised of decursin and decursinol angelate (D/DA) in rats. For the oral acute toxicity test, Sprague-Dawley (SD) male and female rats were gavaged with two doses of D/DA (200 and 2,000 mg/kg body weight) and then observed for any toxic symptoms for 2 weeks. The LD$_{50}$ value for the rats was greater than 2,000 mg/kg body weight for both male and female rats, which indicates that there were no toxic symptoms induced by doses of up to 2,000 mg/kg body weight. For the subacute toxicity study, rats were treated with D/DA at doses of 2 and 20 mg/kg body weight once a day for 30 days. There were no significant changes in body weight and food intake observed during the subacute toxicity study. In addition, no differences were observed between the control and treated groups when urinalysis was conducted or when hematology and biochemical parameters were evaluated. Finally, histopathological examination of the organs did not reveal any lesions in the control or treated groups. Taken together, these findings indicate that D/DA is safe and non-toxic.

• Biomolecules & Therapeutics
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• v.2 no.3
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• pp.247-255
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• 1994

As a series of safety studies of DA-3030, a new rhO-CSF, its local irritancy was examined in the rabbits after the following treatment; application into the conjunctival sac of the eye(single), subcutaneous injection(single), intramuscular injection(single), and intravenous injection(8-day repeated). In addition, paravenous irritation of DA-3030 was investigated in mice. The results obtained were as follows. 1. In the result of ocular irritation test, 0.03% solution of DA-3030 could be considered as a non-irritating material. 2. The local irritation of DA-3030 by an injection of 0.5mι of its solution subcutaneously or intramuscularly was negligible and not so much different from that of saline. 3. In the vascular irritancy test, macro- and microscopic observations revealed that the irritating activity of DA-3030 in blood vessels was not different from that of saline when they were injected once a day into vein retroauricularis of rabbits for 8 days.4. The paravenous administration of DA-3030 did not induce any abnormal changes at injection sites except mild swelling in 1 mouse at 3 hours after injection which was thought to be due to slow absorption. The above-mentioned results suggest that DA-3030 has no irritating activity when injected through intravenous or subcutaneous route for clinical practice as 0.03% solution.

• Bulletin of the Korean Mathematical Society
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• v.60 no.5
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• pp.1265-1280
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• 2023

The aim of this paper is to investigate Betchov-Da Rios equation by using null Cartan, pseudo null and partially null curve in Minkowski spacetime. Time derivative formulas of frame of s parameter null Cartan, pseudo null and partially null curve are examined, respectively. By using the obtained derivative formulas, new results are given about the solution of Betchov-Da Rios equation. The differential geometric properties of these solutions are obtained with respect to Lorentzian causal character of s parameter curve. For a solution of Betchov-Da Rios equation, it is seen that null Cartan s parameter curves are space curves in three-dimensional Minkowski space. Then all points of the soliton surface are flat points of the surface for null Cartan and partially null curve. Thus, it is seen from the results obtained that there is no surface corresponding to the solution of Betchov-Da Rios equation by using the pseudo null s parameter curve.

• Iberoamérica
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• v.11 no.1
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• pp.39-70
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• 2009

Este estudo tem por objetivo analisar, do ponto de vista da alquimia, O Alquimia, considerado uma das obras mais representativas do escritor brasileiro Paulo Coelho. Para tanto, começamos a observar as características da alquimia à qual o próprio autor disse que dedicava 11 anos para estudá-la por volta da década de 1970 quando o mundo estava sofrendo grandes mudanças sociais. Desta observação, entendemos que, sendo uma projeção do 'inconsciente' conforme diz C. G. Jung, a essência da alquimia consiste resumidamente nos seguintes conceitos: união de elementos opostos, transformação e salvação. A partir daí, tentamos estudar como esses coneitos estão diluídos na estrutura de O Alquimia e como a experiência do Paulo Coelho com a alquimia interioriza-se no mundo literário dele. E entendemos que os conceitos simbólicos da alquimia e as mensagens que a experiência do escritor com a alquimia configura em suas obras literárias compartilham a quase mesma dimensão semântica. Percebemos também que em alguns aspectos as idéias do escritor consolidadas em torno da sua pesquisa da alquimia vão às vezes além dos conceitos genéricos da alquimia, atingindo assim uma dimensão bastante peculiar e fundamental para compreender melhor o seu universo literário: para ele que se declara católico, Deus não é católico nem islâmico, nem budista, nem hinduista. Por exemplo, ele diz que Deus é um só e é que o Seu nome varia dependendo da religião, porque cada religião precisa escolher um nome para chamá-lo.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.387-393
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• 2011

We analyzed the particle size distributions of three commercially available Devarda's alloy (DA) products, tested the nitrate recoveries of each particle size category, and examined the amounts of DA required for 100% recovery by varying $NO_3$-N concentration from 0.5 to 10 mg. We observed that use of DA coarser than 200 mesh resulted in poor analytical recovery (<80%). While the tested alloys were considered to be fine enough (>90% of the particles were less than 100 mesh), the recovery dramatically declined from 80% to 10% in a high concentration range (4 to 10 mg N). Satisfactory recovery was obtained by increasing the amount of finer DA (less than 300 or 450 mesh). However, there was no quantitative relationship between the amount of fine DA and nitrate recovered. Generally, the amount of nitrate reduced per unit DA decreased as the recovery efficiency declined. These results suggest that a sufficient amount of DA must be determined based on particle size distribution, and that treatment of at least two levels of DA and comparison of the subsequent change in nitrate recovery is required for soils containing high levels of nitrate. In addition, further studies are encouraged to account for the observed stoichiometric dis-equivalence of recovered nitrate N per unit mass of DA.

• Toxicological Research
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• v.14 no.2
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• pp.193-203
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• 1998

The toxicity of DA-125. a new anthracycline anticancer agent, on the male reproductive system was studied in Sprague-Dawley rats. Forty male rats were rando$m\ell$y assigned to Jour groups with ten rats in each group and given single intraveneous doses of DA-125 at dose levels of 0. 12.5. 25. and 50 mg/kg body weight. On day 56 after treatment the animals were allowed to mate. and their male reproductive Junctions and organs were examined in detail. Copulated females were sacrificed on day 20 of gestation for examination of embryo-fetal development. One out of ten rats in the 50 mg/kg group died on day 12 after treatment. Clinical signs such as emaciation. sedation, anorexia. swelling. dark material around eye. alopecia. and diarrhea were observed in the 25 and/or 50 mg/kg groups. Reduction in the body weight gain. decrease in the absolute weights of testes. epididymis and seminal vesicles. and/or decrease in the number of testicular sperm heads were also found. Although histopathological changes such as atrophy of seminiferous tubules. loss or decrease of spermatogenic cells. exfoliation of spermatogenic cells. vacuolization of Sertoli cells. decrease of sperm. and/or increase of necrotic spermatogenic cells in epididymal ducts were observed. no adverse effects on the motility and morphology of epididymal sperm. copulation index. fertility index. and embryo-fetal development were detected in the 25 and 50 mg/kg groups. There were no evidences of male reproductive toxicity in the 12.5 mg/kg group. These results show that single intravenouse doses of DA-125 produce significant dose-related testicular atrophy. histopathological changes. and oligozoospermia in rats and $LD_{10}$ for DA-125 appears to be 50 mg/kg body weight.