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http://dx.doi.org/10.5657/KFAS.2018.0135

Immuno-potentiating Activities of Enzymatic Hydrolysate of Japanese Mud Shrimp Upogebia major  

Lee, Ji-Hyun (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Yang, Ji-Eun (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Song, Jae-Hee (Tidal Flat Research Center, National Institute of Fisheries Science)
Maeng, Sang-Hyun (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Kim, So-Yeon (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Yoon, Na-Young (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.2, 2018 , pp. 135-141 More about this Journal
Abstract
This study investigated the immuno-potentiating activities of Japanese mud shrimp Upogebia major. We examined the effects of enzymatic hydrolysate from U. major on the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) and on the expression of pro-inflammation cytokines including $TNF-{\alpha}$, IL-6 and $IL-1{\beta}$ in RAW 264.7 cells. The treatment of six enzymatic hydrolysates of U. major (alcalase, ${\alpha}$-chymotrypsin [${\alpha}-Chy$], trypsin, pepsin, neutrase, protamex) significantly increased the production of NO in RAW 264.7 cells, with ${\alpha}-Chy$ having the greatest effect. This hydrolysate was fractionated by two ultrafiltration membranes at 3 and 10 kDa to created three fractions (below 3 kDa, between 3 and 10 kDa, and above 10 kDa). Of these, the <3 kDa and >10 kDa fractions showed significant increases in NO production. These two fractions also induced $PGE_2$ production in RAW 264.7 cells and showed significant increases in the expression of all cytokines studied. These results suggest that enzymatic hydrolysate from U. major is a potentially useful food material with immune-potentiating effects.
Keywords
Upogebia major; Enzymatic hydrolysate; RAW 264.7 cells; Nitric Oxide; Cytokine;
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