Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Immuno-potentiating Activities of Enzymatic Hydrolysate of Japanese Mud Shrimp Upogebia major

쏙(Upogebia major) 효소가수분해물의 면역증강 효과

  • Lee, Ji-Hyun (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Yang, Ji-Eun (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Song, Jae-Hee (Tidal Flat Research Center, National Institute of Fisheries Science) ;
  • Maeng, Sang-Hyun (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Kim, So-Yeon (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Yoon, Na-Young (Food Safety and Processing Research Division, National Institute of Fisheries Science)
  • 이지현 (국립수산과학원 식품위생가공과) ;
  • 양지은 (국립수산과학원 식품위생가공과) ;
  • 송재희 (서해수산연구소 갯벌연구센터) ;
  • 맹상현 (국립수산과학원 식품위생가공과) ;
  • 김소연 (국립수산과학원 식품위생가공과) ;
  • 윤나영 (국립수산과학원 식품위생가공과)
  • Received : 2018.02.06
  • Accepted : 2018.03.13
  • Published : 2018.04.30
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Abstract

This study investigated the immuno-potentiating activities of Japanese mud shrimp Upogebia major. We examined the effects of enzymatic hydrolysate from U. major on the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) and on the expression of pro-inflammation cytokines including $TNF-{\alpha}$, IL-6 and $IL-1{\beta}$ in RAW 264.7 cells. The treatment of six enzymatic hydrolysates of U. major (alcalase, ${\alpha}$-chymotrypsin [${\alpha}-Chy$], trypsin, pepsin, neutrase, protamex) significantly increased the production of NO in RAW 264.7 cells, with ${\alpha}-Chy$ having the greatest effect. This hydrolysate was fractionated by two ultrafiltration membranes at 3 and 10 kDa to created three fractions (below 3 kDa, between 3 and 10 kDa, and above 10 kDa). Of these, the <3 kDa and >10 kDa fractions showed significant increases in NO production. These two fractions also induced $PGE_2$ production in RAW 264.7 cells and showed significant increases in the expression of all cytokines studied. These results suggest that enzymatic hydrolysate from U. major is a potentially useful food material with immune-potentiating effects.

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References

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