• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.4
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• pp.470-475
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• 2006

In order to elucidate the differences of protein profiles among soybean cultivars, the protein composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 was analyzed by total nitrogen measurement, amino acid analysis and PAGE/densitometry. There were no statistically significant differences in the levels of any amino acid, including aromatic amino acids, between glyphosale-tolerant soybean and the conventional soybean WS82. In the extraction of protein, the SDS/buffer system was more efficient than the defatting/water system. The SDS-PAGE/densitometry analysis showed that there was a similar profile of proteins among cultivars, although the amount of total protein ranged from 380.2 mg/g to 423.9 mg/g. In addition, there was no discernable difference of protein profile between glyphosate- tolerant soybean (total protein amount, 380.2 mg/g) and the conventional soybean WS82 (390.2 mg/g), although the amount of ${\beta}$-conglycinin (55 kDa) was lower in glyphosate-tolerant soybean. Meanwhile, the amount of 25 kDa protein was greater in domestic soybean cultivars than imported ones. Thus, normal PAGE/ densitometry method would be useful to analyze the difference in protein profiles of soybean proteins, and furthermore Evaluate the protein profile of proteins between GMO and conventional soybean.

• Journal of Life Science
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• v.14 no.3
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• pp.501-508
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• 2004

A bacterial strain CJ-3 which produced chitinase was isolated from Korean traditional soy sauce. Using 16S rDNA analysis, the strain CJ-3 was identified as Bacillus atrophaeus. The approximate molecular weight of the putative chitinase enzyme was 31.0 kDa and the enzyme activity was remarkably induced by addition of colloidal chitin (0.5, 1.0, 2.0%). The antioxidant activity was increased 53% by the browning reaction products of B. atrophneus CJ-3. Escherichia. coli lipopolysaccharides (LPS)-induced production of nitric oxide(NO) was reduced up to 45% by the browning reaction product in RAW264.7 macrophage. Inhibition of cell viability in the presence of LPS was recovered to normal level by the browning reaction product. These results suggest that browning reaction of B. atrophaeus CJ-3 plays an important role for activation of immune system. B. atrophaeus CJ-3 exhibited optimum temperature and pH of 37$^{\circ}C$ and pH 7.0∼8.0, respectively. The major intracelluar free amino acid was determined to be glutamate.

• Restorative Dentistry and Endodontics
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• v.46 no.1
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• pp.11.1-11.11
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• 2021

Objectives: The aim of this study was to compare smear layer removal by conventional application (CA), passive ultrasonic irrigation (PUI), EasyClean (EC), and XP-Endo Finisher (XPF), using 17% ethylenediaminetetraacetic acid (EDTA) after chemomechanical preparation, as evaluated with scanning electron microscopy (SEM). Materials and Methods: Forty-five single-rooted human mandibular premolars were selected for this study. After chemomechanical preparation, the teeth were randomly divided into 5 groups according to the protocol for smear layer removal, as follows: G1 (control): CA of distilled water; G2 (CA): CA of 17% EDTA; G3 (PUI): 17% EDTA activated by PUI; G4 (EC): 17% EDTA activated by EC; and G5 (XPF): 17% EDTA activated by XPF. SEM images (×1,000) were obtained from each root third and scored by 3 examiners. Data were evaluated using the Kruskal-Wallis and Dunn tests (p < 0.05). Results: In the apical third, there were no statistically significant differences among the groups (p > 0.05). In the cervical and middle thirds, the experimental groups performed better than the control group (p < 0.05); however, G2 presented better results than G3, G4, and G5 (p < 0.05), which showed no differences among one another (p > 0.05). Conclusions: No irrigation method was able to completely remove the smear layer, especially in the apical third. Using CA for the chelating solution performed better than any form of activation.

• The Korean Journal of Nuclear Medicine
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• v.30 no.3
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• pp.332-337
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• 1996

Purpose : It has been known that the activity of extracellular matrix degradative enzymes such as collagenase correlate well with the metastatic potential of various tumor cells in experimental study. This study was aimed at comparing the activities of type IV collagenase with bone scan findings in patients with breast cancer. Materials and Methods : We retrospectively correlated bone scan findings with the results of immunohistochemical staining for 92kDa, 72kDa type IV collagenase in 28, and 30 patients with metastatic breast cancer, respectively, as well as 23, and 27 patients with primary breast cancer, respectively. The immunohistochemical staining was performed with tissue specimens obtained from primary or metastatic breast tumor lesions. The amounts of the enzyme were graded from 0 to 4 and scored by multiplication with the percentage of tumor cells. The confidence of bone scan interpretation for metastasis was also scored from 1 to 5 with increasing probability. Results : There was a significant difference in enzyme scores between patients with and without metastasis. In patients with primary breast cancer group, the frequency of patients with enzyme score of less than 170 were 96%(26/27) and 100%(26/26) with 92kDa and 72kDa collagenase, respectively. In contrast, in patients with metastatic breast cancer group, the frequency of patients with enzyme score of more than 200 were 93%(28/30) and 87%(26/30) with 92kDa and 72kDa collagenase, respectively. All patients with each enzyme score of less than 170 show no active bony metastasis, however, there were variable bone scan findings in patients with each enzyme score of more than 200. Conclusion : Bone scan is useful to confirm, localize or follow up of bony metastasis in patients with each enzyme scores of more than 200. Acitve metastatic lesions were hardly seen on the bone scintigraphy in patients with collagenase scores of less than 170.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.4
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• pp.282-291
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• 2006

Effects of the molecular weight and type of chitosans on shelf-life of Makkulli were evaluated during 18 days of storage at $25^{\circ}C$. Two types of chitosans were studied: ${\alpha}$-chitosans with 11 different molecular weights (water-soluble, Mw = 1, 8, 22, 43, 67 and 616 kDa; acid-soluble, Mw = 282, 440, 746, 1,110 and 2,025 kDa) and ${\beta}$-chitosan (acid-soluble, Mw = 577 kDa). Acid-soluble chitosans were applied as a form of chitosan-ascorbate. All chitosans were added to Makkulli at 0.002% concentration, the optimum concentration established in a preliminary test. Among 12 chitosans, the ${\alpha}$-chitosans with 22 and 440 kDa exhibited stronger antimicrobial effects than did other ${\alpha}$- and ${\beta}$-chitosans. The results for pH, acidity, alcohol concentration, viable cell counts, and sensory evaluation suggested that addition of ${\alpha}$-chitosans with 22 and 440 kDa increased the shelf-life of Makkulli by almost 1 week at $25^{\circ}C$ compared with that of control (without chitosan) and other chitosan-added groups. Extension of Makkulli shelf-life by 1 week is fairly significant in view of the magnitude of the total amount of Makkulli produced in Korea.

• Food Science of Animal Resources
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• v.30 no.2
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• pp.269-276
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• 2010

The objective of this study was to determine the effects of residual nitrite contents, chitosan with different molecular weight and nitrite addition on emulsified sausage during cold storage. Six types of sausages were evaluated: control, 0.5% 50 kDa chitosan (T1), 0.5% 200 kDa chitosan (T2), 150 ppm nitrite (T3), 0.5% 50 kDa chitosan+150 ppm nitrite (T4), and 0.5% 200 kDa chitosan+150 ppm nitrite (T5). Each type of sausage was tested in triplicate and assigned to one of four storage periods: 0, 10, 20 and 30 days. As the storage time increased, the presence of chitosan and nitrite resulted in decreased residual nitrite value and increased pH (in control and T2), TBARS (thiobarbituric acid reactive substance) values, and total plate counts (TPC). Values for pH, TBARS, residual nitrite and total plate counts decreased significantly in response to the addition of chitosan and nitrite relative to the control (p<0.05). T5 was redder than the control (higher CIE$a^*$) at 30 d; however, no difference in the CIE $L^*$ and $b^*$ values was observed. T5 was significantly (p<0.05) more effective at delaying lipid oxidation when compared to the other treatment groups. T5 presented TPC that was significantly lower (p<0.05) than the other groups after three days of storage. In addition, the use of chitosan and nitrite in combination had much better antioxidant and antimicrobial effectiveness than other treatment groups. In conclusion, this study demonstrates that the addition of 0.5% 200 kDa chitosan and 150 ppm nitrite in combination with emulsified sausages tended to improve antioxidative and antimicrobial effects during storage when compared to other treatment groups.

• Journal of Korea Soil Environment Society
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• v.5 no.1
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• pp.33-43
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• 2000

For the consideration of phytoremediation, TEX>$Cd^{2+}$ and Pb$^{2+}$ were analysed in the soil of the habitats and the leaf stem and root of Persicaria thunbergii in the different localities of Bong-Dong river In the soil and plant samples of research areas, TEX>$Cd^{2+}$ was not detected but, $Pb^{2+}$ detected as follows; about 7.5~15.5$mu\textrm{g}$/g in the soil of habitats, about 11.7~18.4 $mu\textrm{g}$/g in the leaf, about 7.~15.5$mu\textrm{g}$/g in the stem and about 89.1~193.6$\mu\textrm{g}$/g in the root of P. thunebrgii and the correlation coefficient value between the $Pb^{2+}$ contents in soil and P. thunbergii was 0.814(>t12, 0.01). After P thunbergii was treated with Cd(NO$_3$)$_2$and Pb(NO$_3$)$_2$of 5 and 10mM, the bioaccumulation of TEX>$Cd^{2+}$ and $Pb^{2+}$ in the leaf of plant, the remaining mass of heavy metals and the variation of pH in the soil, and the increasing rate(%) of phytochelatin in plant were examined. The concentrations of TEX>$Cd^{2+}$and $Pb^{2+}$ in the leaf as follows, in the case of TEX>$Cd^{2+}$ about 0.82~2.79$\mu\textrm{g}$/g and in $Pb^{2+}$, about 2.87~8.08$\mu\textrm{g}$/g. The remaining mass of heavy metals and the variation of pH in the cultured soil decreased as follows; about 77.1% and pH6.39 in TEX>$Cd^{2+}$ 5mM, about 90.2% and pH5.79 in TEX>$Cd^{2+}$ 10mM, about 81.1% and pH6.00 in $Pb^{2+}$ 5mM and about 85.7% and pH5.80 in Pb$^{2+}$ 10mM. The phytochelatin were increased in plant samples treated with 10mM Cd(NO$_3$)$_2$and Pb(NO$_3$)$_2$as follows; about 259% by TEX>$Cd^{2+}$ and about 305% by Pb$^{2+}$ be compared with control. and the molecular weight(da) of these phytochelatins were estimated about 4,300~8,600da in the case of the treatment of TEX>$Cd^{2+}$ and about 3,200~9,700 in $Pb^{2+}$./TEX>.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.3
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• pp.213-223
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• 2022

In this study, the anti-inflammatory and antioxidant effects of aerial parts of Rumex acetosa L. extract were confirmed to prevent various inflammatory diseases and skin aging caused by excessive oxidative stress. As a result of ABTS assay, it was confirmed that the radical scavenging ability increased in a concentration-dependent manner. ROS inhibitory ability was confirmed through DCF-DA assay, and concentration-dependent inhibition of ROS production was confirmed. The effect of inhibiting cell nuclear damage according to ROS was confirmed through DAPI staining. In addition, it was confirmed that the mRNA expression levels of iNOS and COX-2 were inhibited in a concentration-dependent manner through qPCR. As a result of confirming the protein levels of iNOS and COX-2 by western blotting, iNOS was significantly decreased at all concentrations, and COX-2 was significantly decreased at 800 ㎍/mL. The inhibitory effect on the production of NO generated by iNOS was confirmed by NO assay, and NO was decreased in a concentration-dependent manner. In addition, phosphorylation of ERK and JNK in the MAPKs signaling pathway were inhibited. Therefore, Rumex acetosa L. has the potential to be used as an anti-inflammatory and antioxidant cosmetic raw material by showing anti-inflammatory and antioxidant effects through the MAPKs pathway.

• Journal of Microbiology and Biotechnology
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• v.7 no.5
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• pp.305-309
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• 1997

Bacillus stearothermophilus No. 236, an effective xylanolytic bacterium, produced an extracellular carboxymethyl cellulase when the strain was grown on xylan. The carboxymethyl cellulase was purified to homogeneity as judged by SDS-PAGE and zymogram, The carboxymethyl cellulase had a pI of 4.0, and a molecular mass of 95 kDa. The highest level of enzyme activity was observed at pH 6.5 and $60^{\circ}C$. The $K_m$, and $V_{max}$ values of the enzyme to carboxymethyl cellulose were 20.8 mg/ml and $0.63 {\mu}mole$/min/mg protein, respectively, The enzyme was found to act also on filter paper and xylan as well as carboxymethyl cellulose. Therefore, it is expected that this xylanolytic strain isolated from soil could be efficiently used for xylan biodegradation.

• Proceedings of the Korean Society of Computer Information Conference
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• 2023.01a
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• pp.243-246
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• 2023

'서비스를 이용하겠다고 예약을 했지만, 취소나 연락 없이 장소에 나타나지 않는 행위'를 가리키는 일명 노쇼(No-Show, 예약부도)가 사회적 문제로 떠오르고 있다. 이는 음식점을 비롯하여 열차, 고속버스, 병원, 미용실 등 예약 시스템이 갖춰진 사회 곳곳에서 발생하여 사회적·경제적 손실을 일으키고 있다. 그리고 이러한 노쇼는 자영업자의 경제적 손실로 직결되어 이들의 생존을 위협하기에 이르렀다. 음식점의 경우 노쇼로 인해 식재료 손실과 함께 해당 테이블에 다른 고객의 예약을 받지 못하는 2차 피해 발생이 가능하다는 점에서 타 업종에 비해 리스크 부담이 상당하다. 본 연구는 기존 식당 예약 웹 어플리케이션의 경제성·편의성·접근성을 취하는 동시에, 예약금 제도를 통해 노쇼의 비중을 줄이고, 노쇼가 발생한 고객 현황을 계측하여 관리할 수 있는 관리자 권한의 기능을 제공해 손실을 최소화하도록 돕는 웹 어플리케이션을 개발하는 것에 목적을 두고 있다.