• 사상체질의학회지
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• 제1권1호
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• pp.125-138
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• 1989

In order to investigate the effect of Row ginseng (Ra. G.; from $K{\acute{u}}msan$ province, Korea), White ginseng (W.G.; from $K{\acute{u}}msan$ province, Korea), and Red ginseng (Re. G.; form $K{\acute{u}}msan$ province, Korea) on immune response, the author used ICR mice having a body weight of about 20g as experimental animals dividing them into four groups-Saline, Ra. G, W.G., Re. G group. Delayed type hypersensitivity(DTH) and rosette forming cells(RFC) for cell-mediated immune response, hemagglutinin(HA) titers, hemolysin (HL) titers for humoral immune response were measured at 24 hours after challenge, The results were summarized as follows: 1) DTH was increased in all of the treated group as compared with the Saline group, with statistical significance(W.G.> Re. G. > Ra. G.). 2) RFC was increased in all of the treated group as compared with the Saline group, with statistical significance(W.G. > Re. G. > Ra. G.). 3) HA titer was increased in all of the treated group as compared with the Saline group, with statistical significance (Re. G.> W. G.> Ra. G.). 4) HL titer was increased in all of the treated group as compared with the Saline group, with statistical significance (Re. G. > W. G.> Ra. G.). Through the experimental study in ICR mice, these findings suggest that Raw ginseng, White ginseng and Red ginseng enhance both cell-mediated and humoral immune response with statistical significance.

• Journal of Acupuncture Research
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• 제18권2호
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• pp.136-149
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• 2001

Objetives : This study was done to evaluate the antiallegic effect of medicinal acupuncture solution of Acanthopanax senticosus(Rupr. et Maxim;abbreviated as ASMA), studies were done experimentally. Methods : For this aim MTT assay, anaphylaxis reaction, DTH, histamine release, IgE production and vascular permability was evaluated. Results : ASMA didn't show an effective cytotoxicity on the mouse lung fibroblast. It insignifcantly suppressed histamine release from mast cells induced by compound 48/80, while it significantly reduced IgE production and SRBC-challenged DTH(Delayed type hypersensitivity). ASMA effectively suppressed anaphylactic reaction induced by compound 48/80. However, it didn't inhibit vascular permeability and contact dermatitis significantly. Conclusion:Medicinal acupucture solution had antialletgic effects. Acanthopanax senticosus medical acupuncture depress the allergy reaction. Especially in anaphylactic type, the effect was good.

• Journal of Preventive Medicine and Public Health
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• 제28권1호
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• pp.27-42
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• 1995

수은이 마우스의 세포성 및 체액성 면역반응에 미치는 영향을 밝히고자 실험을 통해 얻은 결과를 다음과 같이 요약하는 바이다. $10^{-2}mM$이상의 농도에서 수은은 시험관내의 마우스 비장세포에 대한 명백한 독성 작용(toxic effect)을 나타냈다. 수은에 의한 마우스 생존을(survival rate)은 0.8mg(per 100g body weight) 이하의 농도에서는 100%생존율을 나타냈으며, 0.9mg 이상의 농도에서는 5일 이내에 100% 치사율을 나타냈다. Subtoxic 농도의 수은 투여에 따른 Arthus 및 DTH반응의 결과는 $Arthus(T_3)$반응에 있어서 수은 투여군과 대조군 사이에 유의한 차이가 나타나지 않았던 반면, $T_{24},\;T_{48}$의 DTH반응은 수은 투여군이 대조군에 비하여 억제되었다. 수은 투여 마우스의 면양적혈구(SRBC)에 대한 항체반응을 측정한 결과 면양적혈구에 대한 총응집소가는 수은 투여 농도에 의존적으로 대조군의 값에 비해 억제되었다. 시험관 내에서 마이토젠에 대한 비장세포의 증식 반응은 수은 농도에 의존적으로 억제되었다. 수은 투여군의 IgE 생산은 농도 증가에 따라 $1.7\sim2.0$배의 수준으로 증가하였으며, $IgG_1$의 경우도 증가의 경향을 보였으나 대조군에 비해 큰 차는 아니었다. 이상의 결과를 살펴볼 때 수은 중독시 출현할 수 있는 면역반응의 변화로서, 초기에는 세포성 면역반응의 감소가 현저할 것으로 사료되나 중독이 진행됨에 따라 Ig농도의 상승을 제외한 체액성 면역반응의 전반적인 감소가 수반될 것으로 예측된다.

• 대한미생물학회지
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• 제14권1호
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• pp.71-78
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• 1979

Phytohemagglutinia(PHA) 단독투여(單獨投與) 및 PHA와 Cyclophosphamide(CY)의 동시투여(同時投與)가 마우스의 세포성(細胞性) 및 체액성면역(體液性免疫)에 미치는 영향(影響)을 알아보고저 면양적혈구(緬羊赤血球)(SRBC)로 마우스를 면역(免疫)하기 전에 PHA 및 CY를 투여(投與)하여 말초혈액(末梢血液)의 총(總) 백혈구수(白血球數)와 백혈구백분율검사(白血球百分率檢査), SRBC로 유도(誘導)한 족척종창반응검사(足蹠腫脹反應檢査) 및 비장세포(脾臟細胞)의 rosette 형성검사(形成檢査)를 실시(實施)하였으며 아울러 SRBC에 대(對)한 응집소항체가(凝集素抗體價) 및 용혈소항체가(溶血素抗體價)를 측정(測定)하였다. 그 결과(結果) PHA를 투여(投與)한 마우스군(群)의 면역반응(免疫反應)은 대체(大體)로 감소(減少)되었다. CY만을 투여(投與)한 마우스군(群)의 체액성면역반응(體液性免疫反應)은 감소(減少)되었으나 세포성(細胞性) 면역반응(免疫反應)은 증가(增加)되었다. 이에 반(反)하여 CY 투여전(投與前)에 PHA로 마우스를 전처리(前處理)한 경우 체액성면역반응(體液性免疫反應) 뿐만 아니라 세포성(細胞性) 면역반응(免疫反應)도 감소(減少)되었는데 이 면역반응(免疫反應)의 감소(減少)는 PHA를 CY 투여(投與) 5일전(日前)에 전처리(前處理)한 마우스군(群)에서 가장 현저(顯著)하였다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.171-172
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• 2002

Immune responses to infectious microbes and foreign antigens are regulated by a series of interactions among T cells, B cells, and antigen-presenting cells (APCs) such as macrophages (M$\square$) and dendritic cells (DCs). The inverse relationship between antibody production and cell-mediated immune responses such as delayed type hypersensitivity (DTH) was experimentally manipulated by varying the dose, route of administration, and form of antigen used to immunize animals. (omitted)

• 약학회지
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• 제31권6호
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• pp.355-360
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• 1987

The immunopotenciating effects of ethanol extract, butanol fraction and petroleum ether extract of Korean Ginseng on the immunotoxicity of mitomycin C were investigated in mice. A single administration of mitomycin C induced an apparent but relatively transient reduction of spleen weight, hemagglutination titer, Arthus reaction, RFC and natural killer cell activity. Ethanol extract of Ginseng significantly restored spleen weight, HA titer, RFC and natural killer cell activity. Butanol fraction of Ginseng significantly restored HA titer, RFC and natural killer cell activity. Petroleum ether extract of Ginseng very significantly restored spleen weight, Arthus reaction, DTH, RFC and natural killer cell activity.

• Journal of Acupuncture Research
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• 제18권4호
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• pp.125-142
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• 2001

Objective : The purpose of this study is to observe the effect of Aqua-acupuncture with Stephania Tetrandra Solution (ST-AS) on arthritis. Methods : The author performde several experimental items : that isgene expression and secretion of IL-$1{\beta}$, IL-6, TNF-${\alpha}$, MMP-2, production of ROS, paw thickness, DTH, weight of spleen, expression of CD4+, CD8+, CD19+ in the spleen, production of IL-6, TNF-${\alpha}$, examination of histology. Results : The obstain results are summarized as follows. 1. IL-$1{\beta}$, IL-6, TNF-${\alpha}$ gene expression of hFLS were significantly inhibited in treatmentgroup, and gene expression of MMP-2 was not inhibited in treatmentgroup. 2. The secretion amount of IL-$1{\beta}$, IL-6, TNF-${\alpha}$ were significantly inhibited in treatmentgroup. 3. Expression of P-38 MAP kinase and production of ROS were inhibited in treatmentgroup. 4. Treatmentgroup were significantly inhibited the incidence of arthritis, hind paw edema, the index of arthritis and DTH of CIA (collagen II-induced arthritis) mice. 5. Treatmentgroup were significantly decreased splenetic weight and the number of CD4+, CD8+, CD19+ activated cells and secretion aroout of IL-6, TNF-${\alpha}$ of CIA (collagen II-induced arthritis) mice.. 6. Treatmentgroup were expressed form of new bone, synoviumin, new margine in histology imperison to controlgroup. Conclusions : Taking all these observations into account, ST-AS injection is considered to be effective in treating arthritis and put to practical use in future arthritis clinic.

• 대한미생물학회지
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• 제20권1호
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• pp.45-53
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• 1985

This study was undertaken to assess the susceptibility of pigmented and nonpigmented strains of Serratia marcescens to antibiotics and human sera, and the effect of culture filtrates from pigmented and nonpigmented of Serratia marcescens on humoral and cellular immune responses in mice to thymus-dependent and indepependent antigens. Humoral immune response was measured by hemagglutinin (HA) and hemolysin (HE) to sheep red blood cell (SRBC), and Arthus or antibody response to polyvinylpyrrolidone (PVP). The cellular immune response was measured by delayed-type hypersensitivity (DTH) determined by footpad swelling reactin to SRBC. The resistance of pigmented strains of Serratia marcescens to the bactericidal action of heat inactivated human serum was insignificantly greater than that of nonpigmented strains. However, the pigmented strains were significantly more resistant to the bactericidal action of heat-untreated human serum than that of nonpigmented strains. The clinical isolates of Serratia marcestens was also tested for their resistance to several antibiotics. There was no difference between the pigmented and non-pigmented strains in the resistance to carbenicillin. However, nonpigmented strains were more resistant to gentamicin, kanamycin and tobramycin than the pigmented strains. The intraperitoneal administration of culture filtrates from the pigmented or nonpigmented strains into mice caused enhancemented of antibody response to SRBC or PVP, and of DTH to SRBC. Besides, their enhancement of immune responses was more prominent when culture filtrate from the pigmented strains was administered.

• 약학회지
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• 제40권2호
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• pp.202-211
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• 1996

Experiments were performed on male Sprague-Dawley rats to investigate the immunobiological effects on route of administration of amygdalin(AM). Rats were administered orally at 12.5, 25, or 50mg/kg/day of AM or injected wtih 25,50, or 100mg/kg/day of AM intravenously for 2 weeks. Rats were immunized and challenged with sheep red blood cells(SRBC). The results of this study were summarized as follows;(1) In oral administration of AM, body weight gains were significantly increased by 50mg/kg AM as compared with controls, the relative weights of liver and thymus also were significantly increased by 12.5 and 25mg/kg AM. However, 2-mercaptoethanol-resistant hemagglutination titier (2-MER HA), Plaque forming cells (PFC) and rosette forming cells (RFC) were non-dose dependently decreased. Phagocytic activity and delayed-type hypersensitivity (DTH) reaction also were significantly decreased by 50mg/kg AM. (2) In intravenous injection of AM, body weight gains, hemagglutination titer (HA), 2MER-HA, DTH reaction, PFC, RFC and circulating leukocytes were not influenced by AM. However, the relative weights of liver, spleen and thymus were significantly enhanced 100mg/kg AM. These results indicated that oral administration of AM non-dose dependently suppresses humoral and cell-mediated immunity in SD rats, and that intravenous injection of AM is unaffected humoral and cell-mediated immunity, however, the high dose of it significantly enhances phagocytic activity.

• 대한간호학회지
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• 제23권4호
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• pp.528-543
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• 1993

Vitamin E, which has its advocates in the treatment of diabetes mellitus. autoimmune disease, cancer and peripheral vascular and thromboembolic disease, has now been alleged to have a powerful antioxident effect and to affect various biological activities such as fertility factor, inhibition of human platelet aggregation and stabilization of biological membranes. The present study was designed to test whether vitamin I(alpha-tocopherol) can : (1) enhance the hemagglutinin response to sheep red blood cells (SRBC), (2) modulate Arthus and delayed type hypersensitivity(DTH) to SRBC and contact hypersensitivity to dinitrofluorobenzene (DNFB). (3) enhance the mitogenic response of murine splenocyte, (4) decrease the recovery of Cryptococcus neoformans from brain, lung, liver, spleen and kidney of infected mice and (5) have an inhibitory or enhancing effect on the induction of active systemic anaphylaxis(ASA) induced by chicken-gamma globulin (CGG) in mice. Mice were given either intramuscular injections of 0.3ml (300mg) of vitamin I before immunization or were infection for 10 consecutive days or were given by vitamin I esophageal intubation, 0.1ml(100mg), for 20 days before sacrifice for the mitogenic response experiments. It was found that vitamin E treated mice showed a significant enhancement in hemagglutinin response, Arthus reaction and DTH to SRBC and contact hypersensitivity to DNFB. There was no significant difference in the mitogenic response to phytohemagglutinin(PHA), but the response to concanavalin A(ConA) or pokeweed mitogem(PWM) was increased in vitamin E-treated mice. Interestingly, the vitamin E administration before C. neoformans infection decreased significantly the recovery of C. neoformans from brain lung, liver, spleen and kidney of the infected mice as compared with that of the control mice, strongly suggesting that vitamin E pretreatment may increase the resistance of mice to the fungal infection. Unexpectedly, vitamin E administration enhanced the production of CGG -induced ASA. Taken together, it can be concluded that vitamin I administration may in-crease the humoral and cellular immune response and resistance. to C. neoformans infection, but enhance the induction of ASA to CGG. Further studies are necessary to clarify the underlying mechanism accounting for these effects.