• Natural Product Sciences
• /
• 제8권1호
• /
• pp.26-29
• /
• 2002

The MeOH extract of Sorbus commixta (Rosaceae) exhibited strong DPPH radical scavenging activity, and through activity-guided fractionation two antioxidant compounds were isolated and identified as $catechin-7-O-{\beta}-D-xylopyranoside$ (1) and $catechin-7-O-\;{\beta}-D-apiofuranoside$ (2) by physicochemical and spectrometric methods. To evaluate the antioxidant effect of these compounds, some in vitro tests, such as the DPPH radical scavenging activity test, the superoxide radical scavenging activity test and the lipid peroxidation inhibitory activity test, were performed. Compounds 1 and 2 showed stronger activities than both a-tocopherol and butylated hydroxy anisole (BHA) in each assay.

• 한국식품영양학회지
• /
• 제20권4호
• /
• pp.341-348
• /
• 2007

The antioxidative effect and antimutagenic capacity in ethanol extracts of Lentinus edodes were studied for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food by employing biological and biochemical assay. The $IC_{50}$ of MDA with BSA conjugation reaction, lipid peroxidation and scavenging effect on DPPH radical in ethanol extracts of Lentinus edodes showed 74.58 mg/assay, 5.747 mg/assay and 0.939 mg/assay respectively. So, the most effective antioxidative capacity in ethanol extracts of Lentinus edodes was the scavenging effect on DPPH radical, among the method used this study. The indirect and direct antimutagenic effects of ethanol extracts of Lentinus edodes were examined by Ames test using Salmonella typimurium TA98 and TA100. The inhibition rates on indirect mutagenicity mediated by 2-anthramine showed 91.67% in the Salmonella typimurium TA98 and 96.60% in the Salmonella typimurium TA100. The inhibitory effect on direct mutagenicity mediated by sodium azide in Salmonella typimurium TA100 was 22.83%. and mediated by 2-nitrofluorene in Salmonella typimurium TA98 was 5.34%. This data indicates that ethanol extracts of Lentinus edodes have more effective effects on indirect mutagenicity than direct mutagenicity. From this result, it believed to have a possible antioxidative and antimutagenic capacities, and taken for the candidate of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food.

• 생명과학회지
• /
• 제27권1호
• /
• pp.44-49
• /
• 2017

본 연구에서는 일반적으로 분리 및 분석에 가장 빈번히 사용되고 있는 C18 column과 UV 검출기가 장착된 액체크로마토그래피(HPLC)와 항산화 활성 측정에 사용되는 1, 1-diphenyl-2-picryl hydrazyl (DPPH) 라디칼 소거능 측정 방법을 결합한 HPLC-DPPH 동시 측정법의 최적화와 유용성 확인을 약용식물의 추출물을 대상으로 실시하였다. 최종적으로 적용된 HPLC-DPPH 동시 측정법의 유용성은 갈근, 건강, 유근피, 모과, 황기 등 5종 약용식물의 열수추출물과 대조군으로서 ascorbic acid의 라디칼 소거능을 측정하여 확인하였다. HPLC-DPPH 동시 측정에 앞서 추출액 중 고형분 함량을 refractometer를 사용하여 측정함으로써 추출 수율에 따른 활성 차이를 보정할 수 있도록 하였다. 갈근, 모과, 유근피 열수추출물의 라디칼 소거능이 대조군으로 사용된 ascorbic acid와 비교하여 7.77%, 4.71%, 4.19%로서 다른 열수추출물보다 상대적으로 높은 것으로 확인되었다. 이와 같은 측정법은 실제 활성 성분의 분리 및 분석에 있어서 불필요한 시간 및 시약의 낭비를 줄일 수 있는 유용한 수단이 될 수 있을 것으로 판단된다.

• 대한화장품학회지
• /
• 제32권1호
• /
• pp.23-28
• /
• 2006

본 연구는 택사의 화장품 원료로서의 특성을 알아보기 위하여 화장품의 기능들인 항산화, 미백, 세포 손상 회복 및 항염증과 관련된 다양한 실험을 실시하였다. 30, 70, 100% MeOH 용매로 추출한 택사 추출물들은 DPPH법으로 실시한 free radical scavenging assay에서 좋은 활성을 보여주었고, tyrosinase 활성 억제 시험에서도 0.5% 이상의 농도에서 농도 의존적인 활성을 보여주었다. Human fibroblast를 사용한 proliferation assay (MTT assay)에서 각 용매 추출물들은 별다른 효과를 보여주지 못했고 0.05% 이하의 농도에서는 세포 독성으로부터 안전하다는 것을 알 수 있었다. Bl6 melanocyte를 사용한 melanin 생성 억제시험에서 각 용매별 추출물은 독성으로부터 안전한 0.05% 이하의 농도에서 melanin 생성을 40% 이상 억제하는 높은 활성을 보여주었다. 이후 우리는 택사 추출물의 MPLC분리 분획을 실시하여 세 가지 분획을 얻었으며 이들을 대상으로 세포 손상 회복시험과 melanin생성 억제 시험, 염증인자 생성 억제 시험을 실시하였다. 그 결과 분획물들 중 3번 분획물이 세포 손상 회복을 30% 이상 올려주는 좋은 결과를 보여주었고, melanin 생성 억제 시험과 COX-2 생성억제에서도 주목할만한 결과를 보여주었다.

• 대한약침학회지
• /
• 제9권3호통권21호
• /
• pp.97-104
• /
• 2006

Objectives : This study was conducted to compare antibacterial activities and free radical scavenging activity between the Bee Venom and Sweet Bee Venom in which the allergy-causing enzyme is removed. Methods : To evaluate antibacterial activities of the test samples, gram negative E. coli and gram positive St. aureus were compared using the paper disc method. For comparison of the antioxidant effects, DPPH(1,1-diphenyl-2picrylhydrazyl) free radical scavenging assay and Thiobarbituric Acid Reactive Substances(TBARS) assay were conducted. Results : 1. Antibacterial activity against gram negative E. coli was greater in the Sweet Bee Venom group than the Bee Venom group. 2. Antibacterial activity against gram positive St. aureus was similar between the Bee Venom and Sweet Bee Venom groups. 3. DPPH free radical scavenging activity of the Bee Venom group showed 2.8 times stronger than that of the Sweet Bee Venom group. 4. Inhibition of lipid peroxidation of the Bee Venom group showed 782 times greater than that of the Sweet Bee Venom group. Conclusions : The Bee Venom group showed outstanding antibacterial activity against gram positive St. aureus, and allergen-removed Sweet Bee Venom group showed outstanding antibacterial activity against both gram negative E. coli and gram positive St. aureus. For antioxidant effects, the Bee Venom was superior over the Sweet Bee Venom and the superiority was far more apparent for lipid peroxidation.

• Preventive Nutrition and Food Science
• /
• 제11권3호
• /
• pp.198-203
• /
• 2006

The flowers of Chrysanthemum morifolium (Compositae) were investigated for their biofunctional components. Antioxidant compounds were identified from the flower of Chrysanthemum morifolium, through activity-guided fractionation and repeated column chromatographic separations. The structures of the isolated compounds were characterized as chlorogenic acid (1), luteolin (2) and quercitrin (3) by the analysis of physico-chemical and spectral data. Their antioxidant effects were evaluated by assaying for 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity and inhibition of xanthine/xanthine oxidase activity.

• 대한의생명과학회지
• /
• 제26권4호
• /
• pp.296-301
• /
• 2020

The purpose of this study was performed to evaluate antioxidant activity of the Artemisia asiatica Nakai and Moringa oleifera Lam mixture extract. Mixture extracts were manufactured by concentration and compared with a single extract (only the Artemisia asiatica Nakai mixture and only the Moringa oleifera Lam mixture). The experiments conducted Total polyphenol measurements, Total flavonoid measurements, DPPH radical scavenging activty, ABTS radical scavenging activty and LDH assay. The LDH assay assessment shows that all extracts are cells compared to controls. The toxicity was weak. Finally, The antioxidant capacity was rated higher than mixture extract of a single extract. Also, the optimized mixture was determined AM5 (Artemisia asiatica Nakai mixture: Moringa oleifera Lam mixture = 3:1). For extracts of AM5, Total phenol and flavonoid contents were 271.769±18.087 mg/g and 45.384±5.026 mg/g. and DPPH and ABTS scavenging activity were 70.8±6.496% and 77.1±9.634%. Therefore, it is expected that the value of the extract will increase as it increases its antioxidant activity if it is manufactured according to the appropriate ratio.

• 대한의생명과학회지
• /
• 제14권3호
• /
• pp.193-198
• /
• 2008

In order to evaluate on the effect of kaempferol on the cytotoxicity of oxygen tree radicals, XTT assay was performed to determine the cell viability after skin fibroblasts derived from human (Detroit 51) that were treated with various concentrations of hydrogen peroxide $(H_2O_2)$. And also, the effect of kaempferol on the cytotoxicity induced by H202 that was examined by cell viability, lactate dehydrogenase (LDH) activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in these cultures. $H_2O_2$ decreased cell viability in dose-dependent manner in these cultures and the $XTT_{90}\;and\;XTT_{50}$ values were determined at concentration of $35{\mu}M\;and\;90{\mu}M$ of $H_2O_2$ after skin fibroblasts derived from human were treated with $15{\sim}90{\mu}M$ of $H_2O_2$ for 6 hours, respectively. $H_2O_2$ was highly toxic on cultured skin fibroblasts derived from human by toxic criteria of Brenfreund and Puerner (1984). In the protective effect of kaempferol on $H_2O_2$-induced cytotoxicity, kaempferol increased DPPH radical scavenging activity and significantly decreased LDH activity. From these results, it is suggested that oxygen tree radical, $H_2O_2$, was highly toxic on cultured skin fibroblasts derived from human, and also kaempferol of flavonoid showed the protection on $H_2O_2$-induced cytotoxicity.

• 한국자원식물학회지
• /
• 제29권6호
• /
• pp.704-711
• /
• 2016

Tomatoes are considered as one of the main components of daily meals in most households. Thus, it is important to invest in studies enhancing their yield and nutritional value. The study evaluated the effect of wood vinegar (WV) on tomato under greenhouse conditions. Data on fruit number, fruit weight, and plant height were recorded. Total phenolic content (TPC) and antioxidant activity of tomato were determined using the Folin-Ciocalteu reagent and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, respectively. FRR-CF+500x-WV significantly increased the fruit number (86.11%) and fruit weight (81.78%) over the control. Results were comparable to HRR-CF+250x-WV, indicating that conventional fertilizer and WV may have synergistic effect on each other. TPC and scavenging effect on DPPH radical was improved by 250x-WV application. The increase in the phenolic compounds can be attributed to WV as a direct source or as a factor triggering the plants to produce more secondary metabolites with the purpose of increasing natural defenses. The significant effect obtained by applying the full recommended rate of conventional fertilizer on the carotenoid content was due to the availability of the major nutrients needed by the plant. Correlation analysis showed that carotenoids have negative correlation with TPC and DPPH. However, TPC and DPPH showed that these are positively correlated with each other. Tomatoes are excellent source of antioxidants associated with the reduction of some human diseases and improved health. The results provided evidence that WV alone and/or its combination with conventional fertilizers has favorable effects on the quality of tomato.

• Korean Journal of Acupuncture
• /
• 제25권4호
• /
• pp.89-104
• /
• 2008

Objectives : This study was undertaken to determine the in vitro antioxidant activity of the extract of Chilgitang herb-acupuncture solution (CHAS). Methods : The radical scavenging capacity was tested by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hypoxanthine-xanthine oxidase system, DCFH-DA assay, nitric oxide and peroxynitrite generating system. In addition, antioxidant activity on copper and AAPH mediated human low-density lipoprotein (LDL) oxidation was measured by using TBARS assay and relative electrophoretic mobility assay. The amount of total phenolic compounds was assayed by the Folin-Ciocalteu method. Results : CHAS revealed a potent scavenging activity on DPPH radical(82%), superoxide anions(73%), hydroxyl radical(63%), nitric oxide (99%) and peroxynitrite (99%). Moreover, CHAS showed a strong inhibitory effect (59%) on $FeCl_2$-ascorbic acid induced lipid peroxidation of rat liver homogenate. CHAS also markedly inhibited copper(81%) and AAPH(56%)-mediated LDL oxidation, and effectively suppressed the electrophoretic mobility during exposure of human LDL to copper ions. CHAS (82 mg/g) contained higher concentration of total phenolic compounds than that of water extract (45 mg/g) obtained from Chilgitang. Conclusions : These results indicate that CHAS may protect against ROS- or RNS involved diseases, including cardiovascular diseases.