• 생명과학회지
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• 제22권2호
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• pp.156-160
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• 2012

수증기증류법으로 얻은 제주산 배초향의 정유는 DPPH radical 소거활성이 현저하게 뛰어나는 것을 확인할 수가 있었다. 제주산 배초향의 유효성분을 확인하기 위해 정유성분을 GC-MS로 분석한 결과 각 성분의 retention time및 mass spectral data의 표준 물질과의 비교에 의해서 Pulegone, L-menthone, DL-limonene, Isopulegone, beta-caryophyllene, beta-myrcene, estragole. 등의 정유성분이 확인되었다. 이 연구에서 밝혀진 여러 성분들은 천연유기합성물로 항산화 활성이 잘 알려져 있어 이들이 그 유효성분으로 추측된다. 연구결과 제주산 배초향 정유는 식품 보존제 및 항산화 대체 물질로 개발이 적합하다.

• Ocean and Polar Research
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• 제34권4호
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• pp.445-451
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• 2012

The EtOH extracts of red seaweeds (Symphyocladia latiuscula, Chondrus ocellatus and Carpopeltis affinis) and solvent partitioned fractions were investigated for their 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effects and the total phenolic contents were correlated with ABTS and DPPH radical scavenging activities. The EtOH extracts and their solvent partitioned fractions exhibited strong ABTS and DPPH radical scavenging activities. Among the solvent partitioned fractions obtained from n-Hexane (HX), methylenchloride (MC), ethylacetate (EA), and buthanol (BuOH), the HX fraction from C. affinis showed higher radical scavenging activities than other fractions. Total phenolic contents showed significant correlation ($r^2$ = 0.709) with ABTS radical scavenging activity. The results of this study suggest that the strong radical scavenging activity of HX fraction from C. affinis is a promising natural antioxidant for healthcare products.

• Natural Product Sciences
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• 제17권4호
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• pp.267-272
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• 2011

In our search for bioactive phenolics from plants, the culms of Phyllostachys bambusoides has been selected for investigation of anti-cariogenic and 1,1-diphenylpicrylhydrazyl (DPPH) radical scavenging agents based on the initial screening results. Fractionation process of n-hexane and $CHCl_3$ extracts afforded four phenolic constituents, ferulic acid (1), vanillin (2), coniferaldehyde (3), and coniferyl alcohol (4) as guided by their DPPH free radical scavenging activities. Additionally, activity-guided fractionation of EtOAc extract with anti-cariogenic activity has resulted in the isolation of coniferaldehyde (3), 2,6-dimethoxy-p-benzoquinone (5), p-methoxycinnamic acid (6), (${\pm}$)-balanophonin (7), and 6-methoxychromanone (8). The structures of 1 - 8 were determined by spectroscopic data interpretation, and also by comparison of their data with the published values. Phenolic compounds 1 - 4 exhibited similar DPPH radical scavenging activities compared with the synthetic antioxidant, butylated hydroxytoluene (BHT), and compounds 3 and 5 - 8 showed significant antibacterial activity against cariogenic oral streptococci, Streptococcus mutans and S. sobrinus.

• 대한한방소아과학회지
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• 제24권2호
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• pp.126-136
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• 2010

Objectives The purpose of this study was to investigate the antiallergic and antioxidative effects of Chaenomeles Sinensis (CS). Methods CS pretreatments inhibited anti-DNP IgE in RBL-2H3 mast cells for an hour. we measured cell viability, $\beta$-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, and IL-4, TNF-$\alpha$ mRNA expression CS pretreatments inhibited DNP-HSA($10\;{\mu}g/ml$) for ten minutes, we measured Dicholrodihydrofluorescein(DCF) and DPPH radical-scavenging activity in 0.5 mM 1,1-diphenyl-2-picrydrazyl(DPPH) radical solution, 0.1ml, 99% ethanol 0.8ml, and CS 0.1 ml mixed solution. Results 0, 1, 2, 3, 4, 5 mg/ml CS treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, CS treatments also decreased IL-4, TNF-$\alpha$ mRNA expression in RBL-2H3 cells. CS treatments inhibited reactive oxygen species(ROS) and DPPH radical-scavenging activity. Conclusions These results suggest that CS may be useful for the prevention or treatment of allergic disease.

• 한국작물학회지
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• 제50권spc1호
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• pp.216-219
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• 2005

This objective of this study was to determined the activity of antioxidants of DPPH, SOD and total phenolic compounds in mushroom(P. eryngii spp.) extract. The antioxidant acitivity using DPPH showed that strain No. 2007$(31.78\%)$ and strain No.2014$(27.45\%)$ were the highest DPPH inhibition. Among the thirteen mushroom examined, strain No. 2014$(58.30\%)$ and strain No. 2010$(57.19\%)$ had the highest SOD activity. The highest total phenol content were in strain No.2013$(1465.03{\mu}g/g)$ and strain No.2005$(1401.09{\mu}g/g)$. Our study suggest that it may be possible to improve mushroom with high natural antioxidant.

• 한국식품저장유통학회지
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• 제18권6호
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• pp.967-972
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• 2011

Assai 열매추출물의 자유 라디칼 소거능을 측정하기 위하여 DPPH를 이용한 자유 라디칼 소거능 실험을 수행하여 항산화 효과를 측정한 결과, assai열매추출물의 농도가 높을수록 DPPH활성이 뛰어남을 알 수 있었고, ROS를 이용하여 항산화 효과를 확인하였다. 배양된 대식세포에 assai열매추출물을 농도별로 첨가한 결과, 농도가 높을수록 과산화수소에 의해 유도된 산화적 자극이 감소하였다. 또한 세포 생존에 미치는 영향을 알고자 assai열매추출물을 농도별로 첨가하여 24시간후 세포의 형태변화를 MTT assay로 실시한 결과, 산화적 자극에 의해 발생한 세포 손상이 assai 열매추출물의 농도가 높아질수록 감소하는 것이 확인되었다. 따라서 assai열매추출물은 천연 항산화제로서의 가능성을 보였다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 춘계학술대회
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• pp.829-832
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• 2014

본 연구는 발효 홍국마로부터 에탄올 추출물 농도에 따른 생리활성 효과를 조사하기 위하여 실시하였다. 발효 홍국마 에탄올 추출물로부터 색소, DPPH radical 소거활성, 환원력 및 monacolin K 함량을 조사하였다. 그 결과, 기질로서 장마를 Monascus sp. MK805 균주로 발효하였을 때 홍국색소는 29.6(황색), 15.1(오렌지색), 20.4(적색), DPPH radical 소거활성은 72.8%, 환원력은 1.74 및 monacolin K 함량은 480.6 mg/kg으로 각각 조사되었다. 이처럼 발효 홍국마는 항산화 및 항고지혈증 관련 기능성 식품 및 의약품 소재로의 이용 기능성이 높은 것으로 사료된다.

• 대한의생명과학회지
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• 제14권1호
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• pp.33-38
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• 2008

In order to examine oxidative stress of reactive oxygen species and the antioxidant effect of Citri Reticulatae Pericarpium (CRP) extract, human skin melanoma cells were treated with various concentrations of hydrogen peroxide ($H_2O_2$). Antioxidant effect of CRP extract on $H_2O_2$-induced cytotoxicity, cell viability, DPPH-radical scavenging activity and superoxide dismutase (SOD)-like activity. In this study, $H_2O_2$ decreased cell viability of cultured human skin melanoma cells in dose- and time-dependent manners, and then, midcytotoxicity value (MCV) was determined at $60\;{\mu}M$ after human skin melanoma cells were cultured for 5 hours in the media containing $20{\sim}60\;{\mu}M$ of $H_2O_2$, respectively. The $H_2O_2$ was on cultured human skin melanoma cells because MCV of $H_2O_2$ was lower than $100\;{\mu}M$. In the antioxidant effect of CRP extract, CRP extract increased cell viability DPPH-radical scavenging activity and SOD-like activity. From these results, it is suggested that $H_2O_2$ was very toxic on cultured human skin melanoma cells. And also, CRP extract has the antioxidant effect on $H_2O_2$-induced cytotoxicity.

• 생약학회지
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• 제33권3호통권130호
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• pp.219-223
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• 2002

In order to screen new radical scavenging principle which is expected to be antiaging drug lead, we have isolated 160 strains of the marine-derived fungi and investigated 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity for their acetone extracts. The significant activities (>50% Inhibition) were observed in 8 strains of fungi (MFA006, MFA0l4, MFA040, MFA133, MFA139, MFA143, MFA148, MFA153), and among them, MFA153 (Aspergillus parasiticus) showed the most significant radical scavenging activity. The active components were purified by assay-guided isolation to yield two known benzyl alcohols, l53B3 (1) and l53B4 (2), and their structures were determined by physicochemical evidence. Two compounds (1,2) showed the significant radical scavenging activity with $IC_{50}$ values of 0.6 and $1.4{\mu}M$ against DPPH, respectively.

• 동의생리병리학회지
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• 제24권2호
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• pp.236-241
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• 2010

The purpose of this study was to investigate the mechanism of ethanol extract of Agrimonia pilosa Ledeb. (EAP)-reduced melanogenesis and diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity. Agrimonia pilosa Ledeb., a perennial herbaceous plant, has been used as an antihemorrhagic, anthelminntic, and antiinflammatory agents in Chinese herbal medicine. In the present study, we observed that melanin synthesis and tyrosinase activity of B16F10 cells were significantly decreased by EAP. However, EAP could not suppress tyrosinase activity in the cell-free system, whereas kojic acid directly inhibited tyrosinase activity. Furthermore, EAP decreased the protein expression of tyrosinase, tyrosinase-related prootein 1 (TRP-1), and tyrosinase-related prootein 2 (TRP-2). EAP scavenged DPPH radical up to 41% with 100 ${\mu}g/m{\ell}$ concentration. These results suggest that the hypopigmentary effect of EPA was due to regulation of tyrosinase protein.