• 한국약용작물학회지
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• 제24권6호
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• pp.464-470
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• 2016

Background: Valeriana fauriei (Valerianaceae) has been used to as a traditional medicine to treat a variety of symptoms, including headache, insomnia, hypertension, and menstrual irregularity. However, the present study investigates the species' antioxidant activity and its inhibition of oxidative DNA damage, which have yet to be studied. Methods and Results: The antioxidant activity was assessed using radical scavenging assays with 1,1-diphenyl-2-picryl hydrazyl (DPPH) and, 2, 2'-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and a reducing power assay. The total phenol content was also analyzed, and phenolic compounds were detected using HPLC/UV, whereas the inhibitory effect of Valeriana fauriei on oxidative DNA damage was measured using ${\phi}-174$ RF I plasmid DNA cleavage assay. The DPPH and ABTS radical scavenging activity were $75.17{\pm}3.55%$ and $95.83{\pm}0.63%$, repectively, and the reducing power was $93.14{\pm}1.74$ at $200{\mu}g/m{\ell}$. The total phenol content was $10.24{\pm}0.04mg/g$, whereas chlorogenic acid, catechin, caffeic acid and epicatechin were identified using HPLC/UV, and the ${\phi}-174$ RF I plasmid DNA cleavage assay indicated that V. fauriei provided protection against oxidative damage. Conclusions: The results of the present study suggest that V. fauriei has powerful antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. The species, therefore, provides a valuable resource for the development of natural pharmaceutical to treat aging, cancer, and degenerative diseases.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.118-118
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• 2019

Abeliophyllum distichum Nakai belonging to Oleaceae is only species in Korean endemic genus, Abeliophyllum. Abeliophyllum distichum (AD) is divided into various types according to flower shape and color. AD is known to have various colors such as white, pink, and ivory. Recently, light yellow flowers have been registered as new varieties (Okhwang 1ho). To date, various ecological and morphological studies on AD have been carried out, but no studies have been made on the phytochemicals and activities according to various traits. In this study, we analyzed the phytochemicals and antioxidative activities of from four kinds of flowers (white, pink, ivory, light yellow) in full bloom. The contents of phytochemicals such as chlorogenic acid, Hirsutrin, Rutin, Acteoside and Isoacteoside were analyzed by HPLC. Antioxidant activity was evaluated by DPPH, ABTS. As a result, the content of each substance varied according to the flower color. These results will provide basic data for evaluating the usefulness of genetic resources in Korea and developing new functional materials in preparation for the Nagoya Protocol.

• 한국식품과학회지
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• 제51권3호
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• pp.207-213
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• 2019

This study was conducted to investigate the antioxidant contents and activities of twelve vegetable sprouts (broccoli, red radish, radish, mizuna, kale, taatsai, pak choi, Chinese cabbage, turnip, rapeseed, chicory, and alfalfa). The total flavonoid contents of the broccoli, red radish, and radish sprout were $25.36{\pm}0.13$, $25.26{\pm}1.80$, and $25.16{\pm}1.25mg$ CE/100 g FW, respectively, and were significantly higher than those of the other tested vegetables. Radish sprouts had the highest total phenolic content (112.42 mg GAE/100 g FW), followed by red radish and broccoli sprouts. The main polyphenols in the vegetable sprouts were epicatechin and chlorogenic acid, but they varied across sprout varieties. The correlation between total flavonoids and total phenolics for the 12 vegetable sprouts was very high (r=0.926). The total antioxidant activity (DPPH and ABTS radical scavenging activities) was also highly correlated with total flavonoids and total phenolics.

• 한국식품과학회지
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• 제52권6호
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• pp.604-609
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• 2020

This study analyzed the antioxidant contents and activities of the pedicel and sepals from 12 colored cherry tomatoes ('Green Joy', 'TY Item', 'Dotori Red TY', 'TY Sispen', 'KT Orange TY', 'White Joy TY', 'Dotori Norang TY', 'Beta Tiny', 'Blacklin', 'KT Red TY', 'KT Norang TY', 'Black Joy 200') for their potential use as bioactive ingredients. 'Green Joy' had a significantly higher content of total flavonoids (92.55±3.20 mg CE/100 g FW), total phenolics (261.94±8.32 mg GAE/100 g FW), and total antioxidant activity than the other varieties. The main polyphenols were rutin, chlorogenic acid, and methyl gallate. For all 12 samples, the total flavonoids content was highly correlated with the total phenolics content and the total antioxidant activities. Non-edible parts of cherry tomato have high potential as functional food materials because they contain similar or more antioxidants and antioxidant properties than the pulp of tomato and cherry tomato or other fruits.

• Food Science and Biotechnology
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• 제15권5호
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• pp.728-734
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• 2006

The tannins represent a highly heterogeneous group of water-soluble plant polyphenols that may play an important role in antimutagenic and antioxidant properties. We investigated the antioxidant function of tannic acid in comparison to other phenolic compounds including catechin, chlorogenic acid, cinnamic acid, ellagic acid, and gallic acid for their ability to scavenge several stable radicals and reactive oxygen species (ROS) such as ${\bullet}DPPH^+$, ${\bullet}ABTS^+$, hydrogen peroxide, hydroxyl radical, and superoxide radical. The ability of tannic acid to decrease paraquat-induced lipid oxidation in mouse liver and lung through its antioxidant properties was also assessed. The results showed that almost all the tested compounds have stable radical scavenging activity except cinnamic acid. Tannic acid, gallic acid, and ellagic acid demonstrated remarkable ROS scavenging properties toward $H_2O_2$, ${\bullet}OH^-$, ${\bullet}O_2^-$ and especially only tannic acid could inhibit paraquat-induced lipid peroxidation effectively in mouse liver and lung. Based on these results, it appears that increased number of galloyl and ortho-hydroxyl groups enhances the antioxidant activity of phenolic compounds and tannic acid is evaluated as the most effective antioxidant among all the tested compounds. These results suggest that the tannins, especially tannic acid, can be used as therapeutic agent for various diseases caused by ROS.

• 동의생리병리학회지
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• 제18권2호
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• pp.517-521
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• 2004

The total phenolic contents and antioxidative activities of the ethanol and methanol extracts from 9 Korean medicinal herbs were tested for the application of skin care products such as soaf and lotion. For the evaluation of antioxidative activity of the extract the method of electron donating ability with resolution reagent DPPH(1 ,1-diphenyl-2-picryl hydrazyl) and TBARS (Thiobarbituric acid reactive substances) value were used in comparison with α-tocopherol and BHA. As the results, the total phenolic contents of the extracts calculated as chlorogenic acid were ranged from 0.063-0.124 mg/ml. All the medicinal plant extracts showed some appreciable antioxidative effects. Among them, Zanthoxylum piperitum, Cinnamomum cassia and Caesalpinia sappan were shown to have relatively high antioxidative effects comparable with BHA and α-tocopherol..

• 한국자원식물학회지
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• 제31권6호
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• pp.704-713
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• 2018

Roasting has revealed coffee's potentials as a good source of bioactive compounds. This study was done to investigate the quantitative presence and activity of bioactive compounds including caffeine, chlorogenic acid (CGA), amino acids, and antioxidant capacity on Coffea arabica L. (Guatemala finca San Sebastian) and C. robusta L. (India Azad Hind). Analysis was performed on Green Bean (GB) Medium-Light (ML), Medium (ME) and Medium-Dark (MD) samples of both varieties. From the results, caffeine content was highest in ME samples of both varieties. GB samples of both varieties had high CGA content which decreased after increasing roasting time and temperature. Most amino acids in GB samples was highest, however, glutamic acid, valine, tyrosine, isoleucine, leucine and phenylalanine had highest quantitative increase in ME samples for both varieties. $IC_{50}$ of DPPH and ABTS radical scavenging activity was highest in ML samples of both varieties. $IC_{50}$ of reducing power and total phenolic content was highest in GB sample of both varieties but decreased after increasing roasting conditions. Generally Robusta had the highest quantity of bioactive compounds and antioxidant activity. From this study, the optimal roasting condition for coffee is ME above which there is a significant reduction of bioactive compounds and antioxidant activity.

• 대한임상검사과학회지
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• 제47권4호
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• pp.194-202
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• 2015

신령버섯(Agaricus brasilliensis)는 식용과 약용으로 사용되고 있는 담자균류에 속하는 버섯이다. 본 연구에서는 신령버섯의 자실체를 메탄올과 열수를 이용해 추출한 물질의 항산화, 항당뇨 및 nitric oxide 생성 저해 효과에 대한 실험을 수행하였다. 고속액체 크로마토그래피를 이용해 이 버섯 자실체 추출물의 페놀성 화합물을 분석한 결과 gallic acid, procatechuic acid, chlorogenic acid, (-)-epicatechin, vanillin, rutin hydrate, naringin, quercetin, formononetin, biochanin-A 등 총 10종류의 페놀성 화합물이 확인되었다. 항산화 효과 실험에서 메탄올 추출물의 DPPH radical 소거능은 양성대조군으로 사용한 BHT에 비해 낮았지만 비교적 효과가 우수하였으며, 철 이온 제거 항산화 효과는 메탄올 추출물이 양성대조군인 BHT에 비해 모든 실험 농도에서 유의성 있게 높았다. 환원력의 경우 이 버섯의 추출물은 양성대조군에 비해 모두 모든 농도에서 효과가 낮게 나타났다. 항당뇨 실험에서 ${\alpha}$-glucosidase에 대한 메탄올추출물의 저해효과는 1.0 mg/mL와 2.0 mg/mL의 농도에서 각각 54.48%와 78.43%를 보여 양성대조군인 acarbose의 51.77%과 81.81%에 비해 조금 낮았다. 염증저해 효과 실험에서 RAW 264.7 대식세포가 배양되고 있는 배지에 신령버섯 자실체의 메탄올과 열수추출물을 각각 전 처리 한 후 염증 매개 물질인 LPS를 투여하여 메탄올과 열수 추출물의 NO 생성 저해효과를 조사한 결과 추출물의 농도가 증가함에 따라 대식세포에 생성된 NO의 양이 감소하는 경향을 나타내어 높은 농도에서 저해효과가 우수한 것으로 나타났다. 따라서 신령버섯의 자실체에는 항산화, 항당뇨, 및 항염증 효과를 나타내는 천연물질이 함유되어 있어서 이를 섭취할 경우 건강에 유용한 것으로 생각되었다.

• Applied Biological Chemistry
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• 제48권2호
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• pp.178-182
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• 2005

Yerusalem sage를 물과 에탄올로 추출하여 생리 기능성을 알아보았다. 추출물의 총 페놀 함량은 물 추출물이 9.4 mg/g, 알코올 추출물이 11.1 mg/g로 나타났고 추출물의 항산화 효과는 DPPH가 물 추출물과 알코올 추출물 각각 97.6%, 87.2%로 나타났고, antioxidant protection factor는 각각 1.1, 1.6으로 알콜 추출물이 물 추출물보다 높게 나타났다. ABTS는 물 추출물과 알코올 추출물에서 각각 96.8%, 91.4%로 높게 나타났으며 TBARS 값은 $0.9{\times}10^{-3}\;{\mu}M,\;0.3{\times}10^{-3}\;{\mu}M$로 대조구 $1.1{\times}10^{-3}\;{\mu}M$에 비해 malonaldehyde와 thiobarbituric acid가 반응하여 생성되는 복합체 TBARS값이 낮아 추출물이 지방 산화에 영향을 주는 것을 알 수 있었다. Helicobacter pylori균의 항균활성 측정을 위하여 알콜 추출물의 phenolic compound 함량을 $50\;{\mu}g$에서 $200\;{\mu}g$까지 $50\;{\mu}g$씩 disc paper에 증가시켜 첨가한 결과 저해환의 직경이 첨가량이 증가할수록 9 mm, 10 mm, 12 mm, 15 mm로 크게 나타났다. HPLC로 항산화 및 항균력을 가진 페놀산의 존재유무를 분석한 결과 물과 알코올 추출물에서 chlorogenic acid함량이 가장 많은 것으로 나타났다. 이 결과로 Yerusalem sage는 인공 합성 항산화제가 가지는 단점을 보완할 수 있는 천연 항산화제 및 위 십이지장 계통의 질환을 예방하는 기능을 겸비한 기능성 물질로 개발이 기대되었다.

• 한국식품조리과학회지
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• 제21권5호
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• pp.709-716
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• 2005

본 실험은 떫은감을 7, 15, 23, 31시간으로 가열하여 제조한 농축액의 물리화학적 특성과 항산화능, tannin함량을 비교 분석하였고 떫은감 농축액의 조리적성을 제시하기 위해 관능평가를 실시하였으며 그 결과를 요약하면 다음과 같다. 떫은감 농축액의 수분함량은 각각 48.26, 43.16, 36.57, 33.24%로 농축시간이 길어질수록 농축액의 수분함량은 감소하였다. 가열농축에 의해 조단백질, 조섬유, 조회분 함량은 모두 증가하였다. 비타민 C 함량은 열처리에 의해 많은 양이 감소하였으며 농축시간이 길어질수록 감소의 폭이 컸으나 유의적인 차이는 없었다. 농축시간별 당도는 각각 45, 52, 63, $70^{\circ}Brix$였으며 농축시간이 길어질수록 당도와 점도가 증가하였다. pH는 떫은감의 경우 5.41이었으며 23시간 농축액의 pH가 4.71로 가장 낮았다. 농축시간이 길어질수록 명도와 적색도, 황색도가 유의적으로 감소하였다. 유리당은 fructose, glucose, sucrose는 농축시간이 길어질수록 함량이 증가하였으며 maltose는 떫은감이 가장 높았고 농축시간이 길어질수록 감소하였다. 전자공여능은 떫은감과 농축액 모두 92% 이상으로 측정되어 대조구로 사용한 기존의 항산화제인 BHA 0.0002% 첨가구의 전자공여능인 43.3%보다 항산화력이 2배 이상 우수한 것으로 나타났다. 떫은감 농축액의 경우 모든 페놀성 물질의 함량이 증가한 것으로 나타났으며 총페놀성 물질 중 chlorogenic acid가 주를 이루고 있었고 그 다음으로 flavanol tannin(catechin)함량이 높았다. 감농축액에 대한 관능평가는 전반적인 기호도(overall-acceptability)에서 23시간 농축액이 6.83으로 가장 좋게 평가되었고 그 다음으로 31, 15, 7시간 순으로 좋게 평가되었다. 31시간 농축액의 경우에 단맛은 가장 강하면서 쓴맛, 떫은맛, 신맛이 가장 적고 부드럽게 평가되었으나 상대적으로 색과 향이 매우 강해서 기호도가 약간 감소하였다. 반면 23시간 농축액의 경우는 단맛, 쓴맛, 떫은맛, 신맛에 대한 평가가 31시간 농축액과 유의적인 차이가 없었고 상대적으로 색과 향을 적당하게 평가함으로써 전반적인 기호도에서 가장 좋게 평가된 것으로 사료된다. 또한 23시간의 가열 농축은 31시간에 비해 경제적인 면에서도 바람직할 것으로 사료된다. 결론적으로 떫은감을 가열 농축하여 제조한 23시간 농축액은 자연적인 강한 단맛을 제공할 수 있고 동시에 항산화성과 탄닌성분을 많이 함유함으로써 식품조리와 식품가공에 유용하게 활용될 수 있을 것으로 사료된다.