• Title/Summary/Keyword: DPPH 소거능

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Antioxidant Activities of Young and Mature Fruit in Three Asian Pear Cultivars (동양배 품종별 유과기와 수확기 과실의 항산화 활성)

  • Park, Yeon-Ok;Choi, Jang-Jeon;Choi, Jin-Ho;Kim, Myung-Su;Yim, Sun-Hee;Lee, Han-Chan
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.208-213
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    • 2012
  • This study was performed to compare antioxidant activities of 80% EtOH extracts from young and mature fruit of three pear cultivars ('Wonhwang', 'Whangkeumbae', and 'Chuwhangbae') classified by three parts (peel, core, and flesh) of mature fruit. The total phenolics compound and flavonoid contents of 80% EtOH extracts from young fruit were 1.5-2.5 times higher than the mature fruit with great cultivar difference. In particular, the contents of those compounds were highest in 'Chuwhangbae' pear. The total phenolics compound and flavonoid contents of 80% EtOH extracts from mature fruit were the most in the peel, core, and flesh respectively. DPPH radical scavenging and $ABTS^+$ radical scavenging of 80% EtOH extracts from the young fruit were higher than the mature fruit of 'Chuwhangbae' pear. DPPH radical scavenging and ABTS+ radical scavenging of 80% EtOH extracts from mature fruit were the highest in the peel, core, and flesh respectively.

한국 전통 한 그릇 음식(비빔밥) 및 그 재료들의 DPPH 수소공여능과 아질산염 소거능

  • 구성자;조연옥
    • Proceedings of the EASDL Conference
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    • 2003.04a
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    • pp.83-83
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    • 2003
  • Electron donating ability by DPPH and nitrite scavenge were measured in ethanol extracts from raw, simple cooked and seasoned materials of Bibimbab(mixed rice), Korean traditional one-dish meal. Ethanol extracts of raw frugrantedible wild aster showed the highest electron donating abilities(80.28%) by DPPH. When its seasoned materials were compared with raw and simple cooked materials, it showed the highest electron donating abilities by DPPH.(중략)

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Effects of Aralia continentalis Kitagawa on Antiplatelet and Antioxidative Activities. (독활의 항혈소판 및 항산화 효과)

  • Yang, Seun-Ah;Im, Nam-Kyung;Jhee, Kwang-Hwan;Lee, In-Seon
    • Journal of Life Science
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    • v.18 no.3
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    • pp.357-362
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    • 2008
  • The dried roots of Aralia continentalis Kitagawa is known to have the potential for anti-inflammation and anti-rheumatic, but their effects on thrombosis are not clear. In this study, we evaluated the anti-platelet and antioxidative activities of A. continentalis Kitagawa. Methanol extract and its various fractions of A. continentalis Kitagawa inhibited ADP-induced platelet aggregation, and EtOAc fraction showed strongest inhibition in a concentration-dependent manner with a $IC_{50}$ value of 217.7 ${\mu}g/ml$. Moreover, the EtOAc fraction contained 77.7% of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 43.1% of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity at 0.1 mg/ml. In addition, the methanol and EtOAc fraction dose-dependently inhibited thrombin-stimulated platelet adhesion to collagen or fibrinogen. Collectively, these results suggest that the polyphenol-rich EtOAc fraction from A. continentalis Kitagawa can reduce platelet hyperactivation by scavenging free radicals. Thus, the EtOAc fraction of A. continentalis Kitagawa is a potential source for inhibition of platelet-dependent thrombosis.

Antioxidant Effect of Assai Palm Methanolic Extract (Assai 열매 메탄올 추출물의 항산화 효과)

  • Choi, Chul-Yung;Degrandi, Isolde Hoerbe;Cho, Sung-Hwan
    • Food Science and Preservation
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    • v.18 no.6
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    • pp.967-972
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    • 2011
  • The methanolic product of assai(Euterpe oleracea Mart) scavenged the intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. The radical-scavenging activity of assai palm methanolic extract protected the viability of peritoneal macrophage cells exposed to hydrogen peroxide($H_2O_2$). Furthermore, the extract reduced the apoptotic-cell formation induced by $H_2O_2$, as demonstrated by the decrease in the number of hypodiploid cells and in the apoptotic-cell body formation. These results indicate that assai palm methanolic extract has radical-scavenging activity and ameliorates $H_2O_2$-induced cytotoxicity.

Study on Biological Activities of Extracts for Cosmeceutical Development from Lagerstroemia indica L. Branch (화장품 개발을 위한 배롱나무(Lagerstroemia indica Linnaeus) 가지 추출물의 생리활성에 관한 연구)

  • Lee, Byung-Guen;Kim, Jong-Hyeop;Ham, Sang-Gyeong;Lee, Chang-Eeon
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.29-34
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    • 2014
  • The aim of the study was to examine the cosmetic and biological activity of Lagerstroemia indica L. and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. Lagerstroemia indica L. branch was extracted with 70% acetone in water. In the result of DPPH (1,1-diphenyl-2-picryl-hydrazyl) scavenging radical activity, acetone extract of Lagerstroemia indica L. branch were higher than 73% at the 50 ppm concentration. ABTS radical cation decolorization activity by acetone extract were higher than 78% at the 50 ppm. Both examine of DPPH and ABTS showed high antioxidative activities at the 50 ppm. In the result of nitrite scavenging ability, acetone extract were higher than 63% at the 50 ppm. Collagenase inhibition activity by extract were higher than 85% at the 50 ppm. Extract is showed high collagenase inhibition more than comparison group EGCG at all concentration. These results suggest that Lagerstroemia indica L. has a great potential as a cosmeceutical raw material as well as anti-oxidant and anti-inflammatory and collagenase inhibition activity.

Optimization of Blanching Process of Cirsium setidens by Response Surface Methodology and Influence of Blanching on Antioxidant Capacity (표면 반응 분석법에 의한 곤드레 블랜칭 최적 공정 확립 및 항산화 능에 미치는 영향)

  • Jo, Hyeon Seon;Ha, Yoo Jin;Kim, Yeon Tae;Kang, Gil Nam;Yoo, Sun Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.4
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    • pp.777-787
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    • 2016
  • The purpose of this research was the optimization of Cirsium setidens blanched at various conditions by response surface methodology. Conducted to investigate the change of Cirsium setidens DPPH radical scavenging activity, total phenolic compounds and flavonoids during the blanching processes. With two parameters such as blanching time and temperature, response surface methodology and central composite design was used to study the combined effect of blanching time (70 to 170 seconds) and blanching temperature (70 to $99^{\circ}C$). The changes of DPPH radical scavenging activity, phenolic compounds, and flavonoids on blanching process conditions such as temperature and time were evaluated. After blanching, total phenolic compound was determined from 13.00 to 35.48 mg/ml. total flavonoids was determined from 2.31 to 8.38 mg/ml. DPPH radical scavenging activity was determined from 42.10 to 67.14 %. The optimum conditions were determined to be blanching temperature of $85^{\circ}C$ and blanching time of 150 sec.

Antioxidant and α-glucosidase inhibitory effects of ethanolic extract of Ainsliaea acerifolia and organic solvent-soluble fractions (단풍취 추출물 및 분획물의 항산화 및 α-glucosidase 저해 활성 평가)

  • Lee, Eun-Woo;Kim, Taewan;Kim, Hyun-Seok;Park, Youn-Moon;Kim, Seong-Ho;Im, Moo-Hyeog;Kwak, Jae Hoon;Kim, Tae Hoon
    • Food Science and Preservation
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    • v.22 no.2
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    • pp.275-280
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    • 2015
  • Among the naturally occurring antioxidants, polyphenols are widely distributed in various fruits, vegetables, wines, juices, and plant-based dietary sources and divided into several subclasses that included phenolic acid, flavonoids, stilbenes, and lignans. As part of our continuing search for bioactive food ingredients, the antioxidant and ${\alpha}$-glucosidase inhibitory activities of the aqueous ethanolic extract from the aerial parts of Ainsliaea acerifolia were investigated in vitro. The antioxidant properties were evaluated via radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. In addition, the anti-diabetic effect of A. acerifolia extracts was tested via ${\alpha}$-glucosidase inhibitory assay. Furthermore, the total phenolic contents were determined using a spectrophotometric method. All the tested samples showed dose-dependent radical scavenging and ${\alpha}$-glucosidase inhibitory activities. In particularly, the ${\alpha}$-glucosidase inhibitory and radical scavenging properties of the ethyl acetate (EtOAc)-soluble portion from the aerial parts of the A. acerifolia were higher than those of the other solvent-soluble portions. These results suggest that A. acerifolia could be considered a new potential source of natural antioxidants and antidiabetic ingredients. More systematic investigation of the aerial parts of A. acerifolia will be performed for the further development of anti-oxidative and antidiabetic drugs.

Antioxidative Activity of Methanolic Extracts from Seaweeds (해조류 메탄올 추출물의 항산화효과)

  • Kim, Byoung-Mok;Jun, Joon-Young;Park, Yeung-Beom;Jeong, In-Hak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.8
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    • pp.1097-1101
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    • 2006
  • In this study, seven species of seaweeds (Sargassum horneri, Grateloupia filicina, Kjellmaniella crassifolia, Porphyra tenera, Ecklonia stolonifera, Scytosiphon lomentaria, Agarum cribrosum) from Gangwon coast were collected for antioxidative assays. The methanol soluble and insoluble (water soluble) fraction which were prepared from 75% methanol extraction were assayed by DPPH method and deoxyribose assay. The antioxidative activity of methanol soluble fraction of Agarum, Scytosiphon and Sargassum reached to 26.60%, 24.28% and 23.40%, respectively. The antioxidative activities of methanol insoluble fractions of the Kjellmaniella and Porphyra were assayed to 29.70% and 21.01% which were higher than methanol soluble fractions with the values of 9.26% and 8.66%, respectively. The results from DPPH assay and deoxyribose assay showed the methanol soluble fractions of Agarum and Sargassum have strong antioxidative activity. However the methanol insoluble (water soluble) fractions of seaweeds did not show any meaningful antioxidant activity excepted Kjellmaniella and Porphyra.

Antioxidative Activities and Inhibitory Effects on Tyrosinase and Elastase by Water Extracts of Allium sp. (Allium 속 식물 물 추출물의 항산화 활성과 Tyrosinase 및 Elastase 저해 효과)

  • Jia, Yuan;Yang, Ming;Park, Inshik
    • The Korean Journal of Food And Nutrition
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    • v.28 no.2
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    • pp.247-252
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    • 2015
  • Three different Allium plants, including wild garlic (Allium monanthum Max; AMM), onion (Allium cepa L.; ACL) and green onion (Allium fistulosum L; AFL), have been widely used as spicy food in daily life. This study was attempted to investigate the properties of antioxidant, whitening and anti-wrinkle activities in water extracts in Allium plants. The antioxidant, whitening and anti-Wrinkle activities were evaluated by performing DPPH and ABTS radical scavenging activities, inhibitory effects on tyrosinase, and elastase, respectively. The water extract (10 mg/mL) of onion, wild garlic and green onion exhibited DPPH radical scavenging activities of 28.46%, 28.45%, and 15.91%, respectively. The inhibitory effects of tyrosinase activities by wild garlic, green onion, and onion were increased by heating the extracts at $90^{\circ}C$. Additionally, the wild garlic extract showed higher elastase inhibitory activity than those of other plant extracts. These results suggest that water extracts of Allium plants may be useful as potential sources of beauty foods due to higher antioxidant, anti-melanin and anti-wrinkle activities.