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http://dx.doi.org/10.5352/JLS.2008.18.3.357

Effects of Aralia continentalis Kitagawa on Antiplatelet and Antioxidative Activities.  

Yang, Seun-Ah (The Center for Traditional Microorganism Resources (TMR), Keimyung University)
Im, Nam-Kyung (Department of Food Science and Technology, Keimyung University)
Jhee, Kwang-Hwan (Department of Applied Chemistry, Kumoh National Institute of Technology)
Lee, In-Seon (The Center for Traditional Microorganism Resources (TMR), Keimyung University)
Publication Information
Journal of Life Science / v.18, no.3, 2008 , pp. 357-362 More about this Journal
Abstract
The dried roots of Aralia continentalis Kitagawa is known to have the potential for anti-inflammation and anti-rheumatic, but their effects on thrombosis are not clear. In this study, we evaluated the anti-platelet and antioxidative activities of A. continentalis Kitagawa. Methanol extract and its various fractions of A. continentalis Kitagawa inhibited ADP-induced platelet aggregation, and EtOAc fraction showed strongest inhibition in a concentration-dependent manner with a $IC_{50}$ value of 217.7 ${\mu}g/ml$. Moreover, the EtOAc fraction contained 77.7% of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 43.1% of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity at 0.1 mg/ml. In addition, the methanol and EtOAc fraction dose-dependently inhibited thrombin-stimulated platelet adhesion to collagen or fibrinogen. Collectively, these results suggest that the polyphenol-rich EtOAc fraction from A. continentalis Kitagawa can reduce platelet hyperactivation by scavenging free radicals. Thus, the EtOAc fraction of A. continentalis Kitagawa is a potential source for inhibition of platelet-dependent thrombosis.
Keywords
Aralia continentalis Kitagawa; platelet aggregation; platelet adhesion; antioxidant;
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