• Journal of Food Hygiene and Safety
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• v.26 no.3
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• pp.203-208
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• 2011

This study was to evaluate the efficacy of sanitizer concentrations and treatment time against two major toad-borne pathogenic microorganisms such as Escherichia coli and Staphylococcus aureus on a stainless steel surface. As a result, stainless steel, treated with 100 ppm of chlorine showed reduction of E. coli(1.56, 1.49, 1.95 log cfu/25 $cm^2$) and S. aureus(0.49, 0.88, 1.27 log cfu/25 $cm^2$) after 0, 5 and 10 min, but none was not detected in treatment with 200 ppm. The population of E. coli(0.73, 0.90, 1.55 log cfu/25 $cm^2$) and S. aureus(0.37, 1.00, 1.45 log cfu/25 $cm^2$) reduced in 35.5% ethanol treated group, but none was not detected in treatment with 70%. The population was reduced E coli(0.28, 0.64, 1.07 cfu/25 $cm^2$) and S. aureus(0.53, 0.87, 0.99 log cfu/25 $cm^2$) by treatment with 45.5 ppm of hydrogen peroxide, but none was not detected in treatment with 91 ppm. Quarternary ammonium compound with 100 ppm was reduced E. coli(0.82, 1.62, 1.71 log cfu/25 $cm^2$) and S. aureus(0.46, 0.93, 1.38 log cfu/25 $cm^2$), but none was not detected in treatment with 200 ppm. Predictive models of sterilization for all 4 disinfectants were suitable to use with $r^2$ value of higher than 0.94. These models may be of use to food services and manufacture of safe products by controlling E. coli and S. aureus without the need for further detection of the organisms.

• Journal of Mushroom
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• v.14 no.4
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• pp.220-224
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• 2016

Chronic inflammation, which results from continuous exposure to antigens, is one of major reasons for tissue damage and diseases such as rheumatoid arthritis and type 2 diabetes. In this study, we investigated the anti-inflammatory effects of extracts (hexane, $CHCl_3$, MeOH, $MeOH/H_2O$, and $H_2O$) from GW10-45, which is our new cultivar of an edible mushroom Pleurotus ferulae (ASI 2803 and ASI 2778), in RAW264.7 murine macrophages. None of the extracts showed cytotoxicity in RAW264.7 cells and the hexane, CHCl and H extracts reduced nitric oxide (NO) production, an important inflammatory marker, in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Particularly, the extract (CG45) inhibited NO production more than the other extracts did. To elucidate the effects of CG45 on molecular targets involved in pro-inflammatory responses, we performed western blot analysis. Expression of inducible nitric oxide (iNOS) significantly decreased in LPS and CG45 co-incubated cells compared to that in LPS only-treated cells. Additionally, another protein thatplays a critical role in inflammation, was down-regulated in cells treated with both LPS and CG45. In the nuclear factor $(NF)-{\kappa}B$ pathway, phosphorylation of $I{\kappa}B{\alpha}$ decreased in RAW264.7 cells treated with both LPS and CG45. Furthermore, CG45 inhibited the phosphorylation of $NF-{\kappa}B$ in LPS-stimulated RAW264.7 cells. Conclusively, CG45 could suppress pro-inflammatory responses in LPS-stimulated RAW264.7 cells by down-regulating not only the phosphorylation of $NF-{\kappa}B$ and $I{\kappa}B{\alpha}$ but also the expression of iNOS and COX-2 without any cytotoxicity.

• Journal of Food Hygiene and Safety
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• v.30 no.1
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• pp.43-50
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• 2015

In this study, single PCR and multiplex PCR tests were examined for identification of four types of squid species (giant squid, cuttlefish, octopus, beka squid) purchased from fish market as well as aquatic processed products in Busan. To design the specific primers against each species, the nucleotide sequences of the mitochondrial 16s rRNA gene of Architeuthis dux, Todarodes pacificus, Enteroctopus dofleini, Enteroctopus megalocyathus, Uroteuthis chinensis, Uroteuthis duvauceli, Uroteuthis edulis groups were analyzed for the identification of each species registered in the GeneBank (www.ncbi.nlm.nih.gov) and have been used for comparative analysis. In order to obtain the size variation of amplified fragments on multiplex PCR, we designed KOJ-F, OJ-F, OCT-F, HAN-F, ALLR primers for each species. The optimal PCR conditions and primers were selected for four types of squid species to determine target base sequences in its PCR products. In the case of single PCR, giant squid was only amplified by KOJ-F/ALLR primer; cuttlefish was only amplified by OJ-F/ALLR primer; octopus was only amplified by OCT-F/ALLR primer; and beka squid was only amplified by HAN-F/ALLR primer. For multiplex PCR, the mixture of four kinds of genomic DNA (giant squid, cuttlefish, octopus, beka squid) been prepared as a template and used together with the mixture of KOJ-F/OJ-F/OCT-F/HAN-F/ALLR primers in the reaction. By the multiplex PCR, it is confirmed that four samples are correspond to multiple simultaneous amplicon. Finally, we validated the established methods of multiplex PCR in the aquatic processed products. Although the mitochondrial 16s rRNA primers used in this study was useful as a marker for detection of each species among them, the study indicated that the established multiplex PCR method can be more useful tool for monitoring the processed products.

• Journal of the Korean Applied Science and Technology
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• v.37 no.4
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• pp.870-877
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• 2020

The purpose of this study is to compare and analyze the effects of stepping-in-place tempo and roundhouse kick types on response time. Fifteen males participated in this experiment. All have over ten years of experience and hold a forth dan(degree) black belt in taekwondo. The task is when the participants are doing stepping in place they respond to the light stimulus as fast as they can do roundhouse kick. Five different stepping in place tempos (100, 120, 140, 160, and 180 bpm) and four different types of roundhouse kick(front leg body roundhouse kick, front leg head roundhouse kick, back leg body roundhouse kick, and back leg head roundhouse kick) were used. Three measurements were taken for each of the different combinations of conditions for a total of 60 measurements. For data analysis, two-way ANOVA with repeated measures was used and pair-wise comparisons were performed using bonferroni statistics. The results show that there was significant difference interaction effect between stepping in place tempo and roundhouse kick type in the response time. And, there were significant difference in main effect of response time in accordance with stepping in place tempo and roundhouse kick type. The response time of roundhouse kick was the fastest at 160 bpm of stepping in place tempo, but there was no significant difference between 140 and 160 bpm. Front leg body roundhouse kick was the fastest. And, the response time was the fastest when front leg body roundhouse kicked at 140 bpm of stepping in place tempo. Stepping in place tempo between 140 and 160 bpm is the most effective to optimize the response time. And, More effective response time was front leg roundhouse kick as compared with back leg roundhouse kick and, body roundhouse kick as compared with head roundhouse kick. The findings in this study will provide useful information for performance improvement and will help with strategy for taekwondo competition.

• Journal of Nutrition and Health
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• v.43 no.6
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• pp.597-606
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• 2010

This study investigates the change of level of psychosocial factors of decisional balance, processes of change, and self-efficacy after nutrition education on vegetable and fruit intake of children in pre-action stages. Forty-seven 5th grade elementary school children in Yeongi-Gun, Chungnam-Do were categorized into pre-action stages from a total of 71 children by four-stage questions, and then finally into two stages of precontemplation and contemplation & preparation. After 6 weeks nutrition education, recognition of both of pros and cons of vegetable intake were improved in the two groups of precontemplation and contemplation & preparation. In fruits intake, however, recognition of pros was not changed after nutrition education in either stage, but recognition of cons was improved only in the precontemplation stage. However, no difference in any psychosocial variables was found between precontemplation stage and contemplation & preparation stage. After the nutrition education, the score of consciousness raising of vegetable intake belonging to cognitive process of change increased in the subjects of the precontemplation stage, wheres the scores of dramatic relief, self-reevaluation, environment reevaluation and social liberation increased in the group of contemplation & preparation. In fruit intake, however, no change was found except in the environment reevaluation in the precontemplation group. The behavioral process of change of vegetable and fruit intake did not show any change in either group after nutrition education. Self-efficacy scores of vegetable intake of keeping the foods at hand, eating the recommended number of servings and making time to eat increased in the precontemplation group, while in the contemplation & preparation group, self efficacy scores of shopping for a variety of vegetables, instead of keeping the foods at hands, as well as the scores of the rest two items of eating the recommended number of servings and making time to eat increased after nutrition education. However, in case of fruits intake, no difference was shown in the precontemplation stage except an item of keeping the foods at hand. The results indicated that the stage of change was progressed in the pre-action stage after nutrition education and the scores of many psychosocial factors increased in both pre-action stages. It is suggested that the recognition of cons as well as the cognitive process of change and self-efficacy could be best used as indicators of the assessment of nutrition education program for vegetable intake.

• Restorative Dentistry and Endodontics
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• v.32 no.5
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• pp.419-425
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• 2007

The aim of this study was to evaluate the radiopacity and cytotoxicity of three resin-based (AH 26, EZ fill and AD Seal), a zinc oxide-eugenol-based (ZOB Seal), and a calcium hydroxide-based (Sealapex) root canal sealers. Specimens, 10 mm in diameter and 1 mm in thickness, were radiographed simultaneously with an aluminum step wedge using occlusal films, according to ISO 6876/2001 standards. Radiographs were digitized, and the radiopacity of sealers was compared to the different thicknesses of the aluminum step wedge, using the Scion image software. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of each material was determined in immortalized human periodontal ligament (IPDL) cells. The results demonstrated that EZ fill was the most radiopaque sealer, while Sealapex was the least radiopaque (p < 0.05). AH 26, AD Seal and ZOB Seal presented intermediate radiopacity values. All the materials evaluated, except for Sealapex, presented the minimum radiopacity required by ISO standards. The cell viabilities of resin-based root canal sealers were statistically higher than that of other type of root canal sealers through the all experimental time. Further, EZ fill showed statistically lower cell viability in 24 and 48 hours compared to AD Seal and in 72 hours compared to all other resin-based root canal sealers. However, there was no correlation between the radiopacity and cytotoxicity of three resin-based root canals sealers (p > 0.05). These results indicate that resin-based root canal sealer is more biocompatible and has advantage in terms of radiopacity.

• The KIPS Transactions:PartD
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• v.14D no.6
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• pp.573-584
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• 2007

As multimedia information recently increases fast, various types of retrieval of multimedia data are becoming issues of great importance. For the efficient multimedia data processing, semantics based retrieval techniques are required that can extract the meaning contents of multimedia data. Existing retrieval methods of multimedia data are annotation-based retrieval, feature-based retrieval and annotation and feature integration based retrieval. These systems take annotator a lot of efforts and time and we should perform complicated calculation for feature extraction. In addition. created data have shortcomings that we should go through static search that do not change. Also, user-friendly and semantic searching techniques are not supported. This paper proposes to develop S-MARS(Semantic Metadata-based Multimedia Annotation and Retrieval System) which can represent and extract multimedia data efficiently using MPEG-7. The system provides a graphical user interface for annotating, searching, and browsing multimedia data. It is implemented on the basis of the semantic metadata model to represent multimedia information. The semantic metadata about multimedia data is organized on the basis of multimedia description schema using XML schema that basically comply with the MPEG-7 standard. In conclusion. the proposed scheme can be easily implemented on any multimedia platforms supporting XML technology. It can be utilized to enable efficient semantic metadata sharing between systems, and it will contribute to improving the retrieval correctness and the user's satisfaction on embedding based multimedia retrieval algorithm method.

• Journal of dental hygiene science
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• v.14 no.2
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• pp.158-166
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• 2014

This study is trying to grasp the stress of the male high school students and the correlation between the stress according to the oral health important cognitive and self-rated oral health status and number of brushing, emphasizing the need for the education of oral health important, providing the basic data in order to accomplish correctly until the enhance of oral health-related quality of the oral health correct behavior. From May to July 2013, a self administered survey was conducted by the selected by convenience sampling from subjects of two high school located in Chungcheongnam-do 1, 2 grade. The SPSS PASW Statistics 18.0 and Amos 5.0 program had been used for the statistical data analysis. The study results were as follow: 1) Among five areas of stress, the stress of school life was the highest as 2.11 points and the stress of home problem was the lowest as 1.51 points; 2) The significance analysis results between the five areas of stress according to the stress of latent variable and the oral health-related quality of life all showed the significant difference (p<0.001). 3) Oral health-related quality of life was higher as oral health important and self-rated oral health status positive. Furthermore oral health-related quality of life was higher as number of brushing increased; 4) Fit Measures test result of stress, academic level, and family economic level model all showed more than 0.9 in goodness of fit index (GFI), adjusted GFI, normed fit index and root mean square residual and root mean square error of approximation values is all estimated less than 0.1, so it showed good model. From this study, it can be concluded that there is the correlation between stress and oral health-related quality of life.

• Journal of Technologic Dentistry
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• v.29 no.1
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• pp.103-119
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• 2007

The survey on dental health condition and eating habit of local residents in Daegu and Kyungbuk had been conducted. Through the analysis of influence of dental health condition on eating habit I could get the following result. The number of subjects of survey is 630. The period of survey is from June 2006 to September 2006. 1. General quality of subjects is that forty-four point four percent of subjects are male and fiftyfive pointsix percent are female. The age of thirty point one percent of subjects is between 20 and 29. Fifty-six point two percent of subjects are married. Fifty-one point four percent of subjects have above bachelor degree. Monthly income of forty-four point eight percent of subjects is less than one million won. Twenty-four point four percent of subjects are students. Fifty-five point seven percent of subjects are living in big cities. 2. Forty-seven point seven percent of male and fifty-one point eight percent of female answered their subjective dental health condition is bad. Marital status, age and academic background have relationship with answer. Forty-three point nine percent of married subjects and forty-one point five percent of divorced or bereaved subjects said they have bad dental health condition. The older he is or the lower academic background he has subjects think they have bad dental health condition. Forty-seven point four percent of non-educated subjects answered their dental health condition is bad. Forty-six percent of self-employed subjects and subjects who live in the country have tendency to think their dental health condition is bad. 3. About eating habit knowledge, male's knowledge of 2.03$\pm$0.20 is lower than female's of 2.08$\pm$0.21. This shows there is statistically significant difference(p<0.01). 4. There is significant relationship between subjective dental health condition and health condition of subjective. This means subject who has better health condition has also better dental health condition(37.5%). About subjective dental health condition, subject who eats restoratives has worse dental health condition. This shows there is a relationship between dental health condition and eating restoratives(p=0.004) and subject who works out steadily has better dental condition. 5. About relation between dental health condition and eating habit, subject who eats vegetables has worse dental health condition by fifty point seven percent. As cross tabulation result, p-value is 0.002. In level of significance of 0.05, there is statistically significant difference in eating habit. 6. It seems that eating habit has an effect on marital status, age, academic background, income and also on dental prosthetic treatment situation. Many subjects think their dental health condition is bad. About eating habit, subjects who eat meat have better dental health condition. Subjects who drink green tea and fruit juice has better dental health condition than who drink coffee and Balanced diet is good for dental health condition. As eating habit is important for developing dental health, government should make a proper program. Dental health education program especially for elderly, low-eduacted, residents in the country and poor people should be developed. Government, dental health organization, dental health specialist, associated research institution and people work in the press should be concerned and devote to improve quality of life. Primary prevention education will help for dental health.

• Journal of agricultural medicine and community health
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• v.24 no.1
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• pp.49-63
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• 1999

The objective of the study was to examine and compare health behavior between rural area and urban area in Soonchun city. Data were collected through personal interviews from 25, April to 30, May in 1998. Questions were asked to the rural area residents(n=399) and urban area residents(n=149) about their health behaviors, including such as self-recognition of health status, health related behaviors(smoking, drinking, eating habit, and exercising), status of disease and prevention, and utilization of hospital. As we examine the demographic characteristics, rural area residents were more aged(p<0.001) than urban area residents. And the urban residents had higher education(p<0.01), higher income(p<0.01) and higher health care cost(p<0.01) than rural residents. There were difference in health status existed between rural and urban residents. Rural residents had poorer health status(p<0.01) than urban residents, and however urban residents had more anxiety about their health(p<0.01) than rural residents. Comparison of the health related behavior between rural and urban area residents, rural residents were more likely to smoke(p<0.05), less intake of milk(p<0.01), do not exercise(p<0.01), and less try to lose their weight(p<0.01) than urban residents. Rural resident used to suffer from chronic diseases than urban residents(p<0.01). Consideration of health care need for rural residents are required due to the results shown as above. Therefore, the health care center, where most of the rural residents depend on for their treatment and prevention of disease, should make inquiries about resident's health care need and evaluate the important information sources for construction of a health care information system.